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The map label for this gene is cobB

Identifier: 23100614

GI number: 23100614

Start: 3290867

End: 3291577

Strand: Direct

Name: cobB

Synonym: OB3159

Alternate gene names: 23100614

Gene position: 3290867-3291577 (Clockwise)

Preceding gene: 23100612

Following gene: 23100616

Centisome position: 90.64

GC content: 40.51

Gene sequence:

>711_bases
GTGATAAAAGATTGGCTGCAAGAATCTAATTATACCGTTATATTTACTGGGGCTGGCATGTCCACCGAGAGTGGTCTGCC
GGATTTCCGATCTGCTAACACAGGGCTTTGGAAACAGCACGATCCTAGTAAAATTGCTAGTATAGATACACTGAATAATA
ATGTTGAAACATTTATTGACTTTTATCGAGAACGTGTATTGAAAGTAAAAGAATATGGTCCGCATCAGGGGCATTACATC
TTGGCTGAATGGGAAAAGCAAGGCCTTGTCCATTCCATTGTTACACAGAACGTCGACGGATTTCATCAGGCTTCCGGCAG
TAAAATCGTACATGAATTACATGGCACACTGCAAAAGCTACACTGCCAAAGTTGTGGAAAAGAATATAGCAGCAAAGAAT
ATGTAGAGAATGAATATCATTGCGATTGTGGTGGCGTATTACGTCCTTCCATTATTCTGTTTGGTGAGATGCTACCTCAA
GAAGCTTTTCAAACTGCATTTAACGACGCAGAGAAAGCAGATTTATTTGTCGTACTCGGCTCATCATTGACAGTATCGCC
AGCGAATCAAATCCCATTAATCGCAAAAGAAAATGGAGCGAAACTTGTTATCGTTAATCAAGACCCTACACCGTATGATC
AATATGCTGACATGACCATTTCTGATCAGAAAATAGGTGAATTTCTTCGCTCCATTTCCAATGAGGGGTAA

Upstream 100 bases:

>100_bases
TCTATATTTTTAATAACATATCCCCATTTTTCTGTCAATATCGTTGGCGTTTGAAACAGGTTAGCCTGTATAATAGATGC
CACTATTAGGAGGGATAAAA

Downstream 100 bases:

>100_bases
GGACTAGCCTTATCCCTCACTTTTTGCAACAGTTGATAAAGTCACGATTTTAAAAATCTCGCTTCTTCCTTAACAAATTA
AAAGCCGAACTAAATTACCA

Product: NAD-dependent deacetylase

Products: NA

Alternate protein names: Regulatory protein SIR2 homolog

Number of amino acids: Translated: 236; Mature: 236

Protein sequence:

>236_residues
MIKDWLQESNYTVIFTGAGMSTESGLPDFRSANTGLWKQHDPSKIASIDTLNNNVETFIDFYRERVLKVKEYGPHQGHYI
LAEWEKQGLVHSIVTQNVDGFHQASGSKIVHELHGTLQKLHCQSCGKEYSSKEYVENEYHCDCGGVLRPSIILFGEMLPQ
EAFQTAFNDAEKADLFVVLGSSLTVSPANQIPLIAKENGAKLVIVNQDPTPYDQYADMTISDQKIGEFLRSISNEG

Sequences:

>Translated_236_residues
MIKDWLQESNYTVIFTGAGMSTESGLPDFRSANTGLWKQHDPSKIASIDTLNNNVETFIDFYRERVLKVKEYGPHQGHYI
LAEWEKQGLVHSIVTQNVDGFHQASGSKIVHELHGTLQKLHCQSCGKEYSSKEYVENEYHCDCGGVLRPSIILFGEMLPQ
EAFQTAFNDAEKADLFVVLGSSLTVSPANQIPLIAKENGAKLVIVNQDPTPYDQYADMTISDQKIGEFLRSISNEG
>Mature_236_residues
MIKDWLQESNYTVIFTGAGMSTESGLPDFRSANTGLWKQHDPSKIASIDTLNNNVETFIDFYRERVLKVKEYGPHQGHYI
LAEWEKQGLVHSIVTQNVDGFHQASGSKIVHELHGTLQKLHCQSCGKEYSSKEYVENEYHCDCGGVLRPSIILFGEMLPQ
EAFQTAFNDAEKADLFVVLGSSLTVSPANQIPLIAKENGAKLVIVNQDPTPYDQYADMTISDQKIGEFLRSISNEG

Specific function: Modulates the activities of several enzymes which are inactive in their acetylated form

COG id: COG0846

COG function: function code K; NAD-dependent protein deacetylases, SIR2 family

Gene ontology:

Cell location: Cytoplasm (Probable)

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 deacetylase sirtuin-type domain

Homologues:

Organism=Homo sapiens, GI300797577, Length=230, Percent_Identity=33.0434782608696, Blast_Score=113, Evalue=1e-25,
Organism=Homo sapiens, GI6912662, Length=267, Percent_Identity=27.7153558052434, Blast_Score=112, Evalue=4e-25,
Organism=Homo sapiens, GI300795542, Length=223, Percent_Identity=32.7354260089686, Blast_Score=112, Evalue=4e-25,
Organism=Homo sapiens, GI6912660, Length=212, Percent_Identity=34.4339622641509, Blast_Score=110, Evalue=2e-24,
Organism=Homo sapiens, GI13787215, Length=241, Percent_Identity=31.1203319502075, Blast_Score=109, Evalue=2e-24,
Organism=Homo sapiens, GI6912664, Length=241, Percent_Identity=31.1203319502075, Blast_Score=109, Evalue=2e-24,
Organism=Homo sapiens, GI63054862, Length=208, Percent_Identity=34.6153846153846, Blast_Score=107, Evalue=7e-24,
Organism=Homo sapiens, GI300797597, Length=215, Percent_Identity=32.5581395348837, Blast_Score=102, Evalue=4e-22,
Organism=Homo sapiens, GI13775602, Length=214, Percent_Identity=31.3084112149533, Blast_Score=101, Evalue=5e-22,
Organism=Homo sapiens, GI13775600, Length=214, Percent_Identity=31.3084112149533, Blast_Score=100, Evalue=9e-22,
Organism=Homo sapiens, GI300797705, Length=193, Percent_Identity=32.1243523316062, Blast_Score=97, Evalue=2e-20,
Organism=Homo sapiens, GI7657575, Length=226, Percent_Identity=26.5486725663717, Blast_Score=95, Evalue=5e-20,
Organism=Homo sapiens, GI7706712, Length=246, Percent_Identity=29.2682926829268, Blast_Score=81, Evalue=7e-16,
Organism=Homo sapiens, GI215982798, Length=176, Percent_Identity=24.4318181818182, Blast_Score=67, Evalue=1e-11,
Organism=Escherichia coli, GI308199517, Length=210, Percent_Identity=33.8095238095238, Blast_Score=103, Evalue=1e-23,
Organism=Caenorhabditis elegans, GI17541892, Length=223, Percent_Identity=32.2869955156951, Blast_Score=119, Evalue=2e-27,
Organism=Caenorhabditis elegans, GI17567771, Length=259, Percent_Identity=27.7992277992278, Blast_Score=105, Evalue=2e-23,
Organism=Caenorhabditis elegans, GI71990482, Length=259, Percent_Identity=27.4131274131274, Blast_Score=102, Evalue=2e-22,
Organism=Caenorhabditis elegans, GI71990487, Length=261, Percent_Identity=27.2030651340996, Blast_Score=97, Evalue=8e-21,
Organism=Caenorhabditis elegans, GI17505510, Length=238, Percent_Identity=27.3109243697479, Blast_Score=75, Evalue=3e-14,
Organism=Saccharomyces cerevisiae, GI6325242, Length=214, Percent_Identity=30.8411214953271, Blast_Score=103, Evalue=2e-23,
Organism=Drosophila melanogaster, GI24648389, Length=191, Percent_Identity=32.9842931937173, Blast_Score=105, Evalue=3e-23,
Organism=Drosophila melanogaster, GI24645650, Length=238, Percent_Identity=28.5714285714286, Blast_Score=96, Evalue=2e-20,
Organism=Drosophila melanogaster, GI28571445, Length=255, Percent_Identity=30.1960784313725, Blast_Score=91, Evalue=6e-19,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NPD_OCEIH (Q8ELR0)

Other databases:

- EMBL:   BA000028
- RefSeq:   NP_694081.1
- ProteinModelPortal:   Q8ELR0
- SMR:   Q8ELR0
- GeneID:   1018091
- GenomeReviews:   BA000028_GR
- KEGG:   oih:OB3159
- NMPDR:   fig|221109.1.peg.3162
- HOGENOM:   HBG641281
- OMA:   MSTESGV
- ProtClustDB:   PRK00481
- BioCyc:   OIHE221109:OB3159-MONOMER
- GO:   GO:0005737
- HAMAP:   MF_01121
- InterPro:   IPR003000
- PANTHER:   PTHR11085

Pfam domain/function: PF02146 SIR2

EC number: 3.5.1.- [C]

Molecular weight: Translated: 26388; Mature: 26388

Theoretical pI: Translated: 4.96; Mature: 4.96

Prosite motif: PS50305 SIRTUIN

Important sites: ACT_SITE 114-114

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.7 %Cys     (Translated Protein)
1.7 %Met     (Translated Protein)
3.4 %Cys+Met (Translated Protein)
1.7 %Cys     (Mature Protein)
1.7 %Met     (Mature Protein)
3.4 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MIKDWLQESNYTVIFTGAGMSTESGLPDFRSANTGLWKQHDPSKIASIDTLNNNVETFID
CCCHHHHCCCCEEEEECCCCCCCCCCCCCCCCCCCCCCCCCCHHHHHHHHCCCCHHHHHH
FYRERVLKVKEYGPHQGHYILAEWEKQGLVHSIVTQNVDGFHQASGSKIVHELHGTLQKL
HHHHHHHHHHHCCCCCCCEEEEECCCCCHHHHHHHCCCCCHHHCCCHHHHHHHHHHHHHH
HCQSCGKEYSSKEYVENEYHCDCGGVLRPSIILFGEMLPQEAFQTAFNDAEKADLFVVLG
HHHHHHHHCCCHHHHCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHCCCCCCEEEEEEC
SSLTVSPANQIPLIAKENGAKLVIVNQDPTPYDQYADMTISDQKIGEFLRSISNEG
CCEEECCCCCCCEEEECCCCEEEEECCCCCCHHHHCCCEECHHHHHHHHHHHCCCC
>Mature Secondary Structure
MIKDWLQESNYTVIFTGAGMSTESGLPDFRSANTGLWKQHDPSKIASIDTLNNNVETFID
CCCHHHHCCCCEEEEECCCCCCCCCCCCCCCCCCCCCCCCCCHHHHHHHHCCCCHHHHHH
FYRERVLKVKEYGPHQGHYILAEWEKQGLVHSIVTQNVDGFHQASGSKIVHELHGTLQKL
HHHHHHHHHHHCCCCCCCEEEEECCCCCHHHHHHHCCCCCHHHCCCHHHHHHHHHHHHHH
HCQSCGKEYSSKEYVENEYHCDCGGVLRPSIILFGEMLPQEAFQTAFNDAEKADLFVVLG
HHHHHHHHCCCHHHHCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHCCCCCCEEEEEEC
SSLTVSPANQIPLIAKENGAKLVIVNQDPTPYDQYADMTISDQKIGEFLRSISNEG
CCEEECCCCCCCEEEECCCCEEEEECCCCCCHHHHCCCEECHHHHHHHHHHHCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: DNA [C]

Specific reaction: Protein + DNA = Protein-DNA [C]

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 12235376