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The map label for this gene is alr1

Identifier: 23100604

GI number: 23100604

Start: 3280705

End: 3281835

Strand: Reverse

Name: alr1

Synonym: OB3149

Alternate gene names: 23100604

Gene position: 3281835-3280705 (Counterclockwise)

Preceding gene: 23100605

Following gene: 23100603

Centisome position: 90.4

GC content: 40.23

Gene sequence:

>1131_bases
ATGAAAGTAACGAGTTATCGCGATGCATGGGTGGAAGTTTCCTTAGATGCACTAACTTATAATGTACGTCAATTTAAGAA
TCATTTACAGGAAAAGTCAAGGTTGATGGCAGTTGTAAAAGCAGATGGTTATGGTCACGGGGCCGTTCCGATAGCGCGGA
GATCCTTAGAAGCAGGGGCGGAATATCTTGGAGTAGCTTTTATAGATGAGGCACTTCAGATTCGGGAAGCTGGAATATTA
GCTCCAATCTTATTACTTGGTTTTACACCAAGCTACGCTGTAAGAGAAGCTGTTAGACATGACCTCACACTCACCGTATA
TTCGACGGAAACACTGGAAGCGATAAAGGATGCAGCTCGATCTTTAGATAAAAAGGCAAAAATACATATAAAAGTGGATA
GTGGTATGAGTCGGATCGGGTTAAGATCCTCTGAGGAGGTCATCTCTCTAATGACATCACTACAAACGGAAGAAATTGAA
GTAGAAGGGATTTTCACTCATTTTGCGGATGCTGATAATGATGAGAGTGATGCGTATACATATAAACAATTTGAAACATT
TCAAGGGATTATGGAAGCGTTAGAAAATGAAGGTTATTCGATACCAATCAAGCATTGCTGTAATAGTGCAGCAACGATAG
CATTTCCTGACATGCATATGGATATGGTTCGTGTTGGCATTAGTTTATACGGGTTATACCCTGGACAACATTTAAAGGAA
ATATTGTCCTTACAGCAAGTAATGAGCTTTAAAGCAAAGCCTGTGCTCATAAAAGATGTTCCCCCTCATCAACCAATTAG
CTATGGATTAACGTATGAAACAAAGGAAACGTCCAAGATCGCAACACTTCCTATAGGCTATGCGGATGGTTTTTCCCGTT
TGTTATCAAATGTAGGTCATGTGACTGTTCATGGAACAGCGTGTCCCATTGTAGGGAGAATTTGTATGGATCAGTCTATG
ATAGATGTGTCTGAAGTTCAAACGGATGTAACTAAAGACGATATCGTAACCATTTTTGGTGATGAAAATGCGGGGTATAT
ACCGCTCGACGTTGTTGCAGAGCAAATGCAAACGATCCATTATGAAACAGTGTGTCTTATTGGAAAAAGAGTACCAAGAC
ATTACGTGTAG

Upstream 100 bases:

>100_bases
GTTGAAACGATGTTAATAGTTCTCCTATAATTGGTTTAATAATGCTGAAGTTATACAGTTGTTTCATATTAGAGAATGGA
TGTAGTAGGAGGAACAGATG

Downstream 100 bases:

>100_bases
AATAATTTTTCCTTGCTTTCTAGTCGGTCTGAGAGACAGCTGGAAAGTATAGCATTTTAGAAAAGCTTCAATTCTTAAGA
AAAAAATTTACTCAACTAAA

Product: alanine racemase

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 376; Mature: 376

Protein sequence:

>376_residues
MKVTSYRDAWVEVSLDALTYNVRQFKNHLQEKSRLMAVVKADGYGHGAVPIARRSLEAGAEYLGVAFIDEALQIREAGIL
APILLLGFTPSYAVREAVRHDLTLTVYSTETLEAIKDAARSLDKKAKIHIKVDSGMSRIGLRSSEEVISLMTSLQTEEIE
VEGIFTHFADADNDESDAYTYKQFETFQGIMEALENEGYSIPIKHCCNSAATIAFPDMHMDMVRVGISLYGLYPGQHLKE
ILSLQQVMSFKAKPVLIKDVPPHQPISYGLTYETKETSKIATLPIGYADGFSRLLSNVGHVTVHGTACPIVGRICMDQSM
IDVSEVQTDVTKDDIVTIFGDENAGYIPLDVVAEQMQTIHYETVCLIGKRVPRHYV

Sequences:

>Translated_376_residues
MKVTSYRDAWVEVSLDALTYNVRQFKNHLQEKSRLMAVVKADGYGHGAVPIARRSLEAGAEYLGVAFIDEALQIREAGIL
APILLLGFTPSYAVREAVRHDLTLTVYSTETLEAIKDAARSLDKKAKIHIKVDSGMSRIGLRSSEEVISLMTSLQTEEIE
VEGIFTHFADADNDESDAYTYKQFETFQGIMEALENEGYSIPIKHCCNSAATIAFPDMHMDMVRVGISLYGLYPGQHLKE
ILSLQQVMSFKAKPVLIKDVPPHQPISYGLTYETKETSKIATLPIGYADGFSRLLSNVGHVTVHGTACPIVGRICMDQSM
IDVSEVQTDVTKDDIVTIFGDENAGYIPLDVVAEQMQTIHYETVCLIGKRVPRHYV
>Mature_376_residues
MKVTSYRDAWVEVSLDALTYNVRQFKNHLQEKSRLMAVVKADGYGHGAVPIARRSLEAGAEYLGVAFIDEALQIREAGIL
APILLLGFTPSYAVREAVRHDLTLTVYSTETLEAIKDAARSLDKKAKIHIKVDSGMSRIGLRSSEEVISLMTSLQTEEIE
VEGIFTHFADADNDESDAYTYKQFETFQGIMEALENEGYSIPIKHCCNSAATIAFPDMHMDMVRVGISLYGLYPGQHLKE
ILSLQQVMSFKAKPVLIKDVPPHQPISYGLTYETKETSKIATLPIGYADGFSRLLSNVGHVTVHGTACPIVGRICMDQSM
IDVSEVQTDVTKDDIVTIFGDENAGYIPLDVVAEQMQTIHYETVCLIGKRVPRHYV

Specific function: Provides the D-alanine required for cell wall biosynthesis

COG id: COG0787

COG function: function code M; Alanine racemase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the alanine racemase family

Homologues:

Organism=Escherichia coli, GI1790487, Length=373, Percent_Identity=30.2949061662198, Blast_Score=160, Evalue=1e-40,
Organism=Escherichia coli, GI1787439, Length=374, Percent_Identity=30.2139037433155, Blast_Score=139, Evalue=3e-34,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): ALR1_OCEIH (Q8ELR9)

Other databases:

- EMBL:   BA000028
- RefSeq:   NP_694071.1
- ProteinModelPortal:   Q8ELR9
- SMR:   Q8ELR9
- GeneID:   1018093
- GenomeReviews:   BA000028_GR
- KEGG:   oih:OB3149
- NMPDR:   fig|221109.1.peg.3152
- HOGENOM:   HBG712172
- OMA:   SVELWGD
- ProtClustDB:   CLSK302699
- BioCyc:   OIHE221109:OB3149-MONOMER
- BRENDA:   5.1.1.1
- HAMAP:   MF_01201
- InterPro:   IPR000821
- InterPro:   IPR009006
- InterPro:   IPR011079
- InterPro:   IPR001608
- InterPro:   IPR020622
- Gene3D:   G3DSA:2.40.37.10
- PRINTS:   PR00992
- TIGRFAMs:   TIGR00492

Pfam domain/function: PF00842 Ala_racemase_C; PF01168 Ala_racemase_N; SSF50621 Racem_decarbox_C

EC number: =5.1.1.1

Molecular weight: Translated: 41741; Mature: 41741

Theoretical pI: Translated: 5.26; Mature: 5.26

Prosite motif: PS00395 ALANINE_RACEMASE

Important sites: ACT_SITE 40-40 ACT_SITE 268-268

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.3 %Cys     (Translated Protein)
3.2 %Met     (Translated Protein)
4.5 %Cys+Met (Translated Protein)
1.3 %Cys     (Mature Protein)
3.2 %Met     (Mature Protein)
4.5 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MKVTSYRDAWVEVSLDALTYNVRQFKNHLQEKSRLMAVVKADGYGHGAVPIARRSLEAGA
CCCCCCCCCEEEEEHHHHHHHHHHHHHHHHHHHHEEEEEEECCCCCCCCHHHHHHHHHHH
EYLGVAFIDEALQIREAGILAPILLLGFTPSYAVREAVRHDLTLTVYSTETLEAIKDAAR
HHHHHHHHHHHHHHHHCCHHHHHHHHCCCCCHHHHHHHHCCEEEEEEEHHHHHHHHHHHH
SLDKKAKIHIKVDSGMSRIGLRSSEEVISLMTSLQTEEIEVEGIFTHFADADNDESDAYT
CCCCCEEEEEEECCCCHHCCCCCHHHHHHHHHHCCCCEEEEEEEEEECCCCCCCCCCCHH
YKQFETFQGIMEALENEGYSIPIKHCCNSAATIAFPDMHMDMVRVGISLYGLYPGQHLKE
HHHHHHHHHHHHHHHCCCCEEEHHHHCCCCCEEEECCHHHHHHHHHHHHEECCCHHHHHH
ILSLQQVMSFKAKPVLIKDVPPHQPISYGLTYETKETSKIATLPIGYADGFSRLLSNVGH
HHHHHHHHHCCCCCEEEECCCCCCCCEECCEEECCCCCEEEEEECCHHHHHHHHHHCCCE
VTVHGTACPIVGRICMDQSMIDVSEVQTDVTKDDIVTIFGDENAGYIPLDVVAEQMQTIH
EEEECCCCHHHHHHHHCCCCCCHHHHHHCCCCCCEEEEEECCCCCEEHHHHHHHHHHHHH
YETVCLIGKRVPRHYV
HHHHHHHHCCCCCCCC
>Mature Secondary Structure
MKVTSYRDAWVEVSLDALTYNVRQFKNHLQEKSRLMAVVKADGYGHGAVPIARRSLEAGA
CCCCCCCCCEEEEEHHHHHHHHHHHHHHHHHHHHEEEEEEECCCCCCCCHHHHHHHHHHH
EYLGVAFIDEALQIREAGILAPILLLGFTPSYAVREAVRHDLTLTVYSTETLEAIKDAAR
HHHHHHHHHHHHHHHHCCHHHHHHHHCCCCCHHHHHHHHCCEEEEEEEHHHHHHHHHHHH
SLDKKAKIHIKVDSGMSRIGLRSSEEVISLMTSLQTEEIEVEGIFTHFADADNDESDAYT
CCCCCEEEEEEECCCCHHCCCCCHHHHHHHHHHCCCCEEEEEEEEEECCCCCCCCCCCHH
YKQFETFQGIMEALENEGYSIPIKHCCNSAATIAFPDMHMDMVRVGISLYGLYPGQHLKE
HHHHHHHHHHHHHHHCCCCEEEHHHHCCCCCEEEECCHHHHHHHHHHHHEECCCHHHHHH
ILSLQQVMSFKAKPVLIKDVPPHQPISYGLTYETKETSKIATLPIGYADGFSRLLSNVGH
HHHHHHHHHCCCCCEEEECCCCCCCCEECCEEECCCCCEEEEEECCHHHHHHHHHHCCCE
VTVHGTACPIVGRICMDQSMIDVSEVQTDVTKDDIVTIFGDENAGYIPLDVVAEQMQTIH
EEEECCCCHHHHHHHHCCCCCCHHHHHHCCCCCCEEEEEECCCCCEEHHHHHHHHHHHHH
YETVCLIGKRVPRHYV
HHHHHHHHCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 12235376