Click here to switch to the map view.

The map label for this gene is dacC [H]

Identifier: 23100600

GI number: 23100600

Start: 3277004

End: 3278530

Strand: Reverse

Name: dacC [H]

Synonym: OB3145

Alternate gene names: 23100600

Gene position: 3278530-3277004 (Counterclockwise)

Preceding gene: 23100601

Following gene: 23100599

Centisome position: 90.3

GC content: 38.38

Gene sequence:

>1527_bases
ATGAATCGTAAAGCCTTTGTTAGTAAGTTAGTCGTTCTTGTTGGATTAATTGCCATAATTAGTGGGTTGTTATTATTTTA
TCCACATCTTGAAACAAAGATTGACGCCACAGACGAGACGGACATTACACAACTAGAAGATGCAACCTTAGAAGAAAAGT
TAGCTGTCATTTTAGATAACCCTAATTTAGAAGGTGGAATAACTGGGGTTAGCGTGATGGATGCTGCGAATGGTGATCAA
CTATTCTCTAGAAATATTGATATTCGATTACATCCTGCATCAAATATGAAAGTCCTAACTGCTGTATCAGCATTGGAAAC
GCTAGGTCCTGACCATCGGTTTACAACGGAAGTACTGACGGATGGAAAAATACGTGGAAAAGTTCTTCACGGAGATGTAT
ATGTTAAAGGGAAGGGAGATCCTACCTTACTACAATCTGATCTGGAAGCATTCGCTAAGGAGTTAAAGAAACAGGGCATT
CATAAAATTAAAGGTGATTTAGTTGGCGATGATACTTGGTATGATGATGTTCGTCTTTCCCAAGATTTAAACTGGTCCGA
TGAACCGTTCTTCACTGGGGCACAAGTTTCTGCATTAACTATGTCACCAGATGACGATTATGATGCAGGAACCGTTATTG
TGGAGGTCAGACCAAGTGAAAATGGAAAGAAAGCAGAAGTAACATTAACTCCTGAGAATAATTATATATCAATTGAGAAT
AAAACGAAGATGGTCGCTAACGGAGAAGAAAAAGACATTTCAATTGAACGACAACATGGGAATAATACAATTGTAATTGA
AGGAGAAATGCCAAAAGACGGCTCGTTATCCCGGTCTTGGGCATCTGTTTGGGAGCCCACCAATTACGTTATTAGCGTGT
TTAGACAAACTTTAGAAGAGGAAGGCATAACTTTTATAGGGAAATCCAAGGATCGTTTTGAAGAAACTCCTTCTTCTGCT
GAAATACGTGTTGCTAAAGAATCGATGCCGTTAAAAGAACTATTAATACCATTTATGAAATTAAGTAATAATGGACATGG
GGAAACGCTAGTAAAAGAAATGGGAAAAGTGGTTGAGGACGATGGTAGTTGGGATGAAGGATTAGTAGTAATGGAAGATG
TTCTAGGTGAATTGGGGTTGAACATGGATACTATATTATTACGAGATGGTTCAGGAATGTCGCATAAGAATTTTATTCCT
GCGGTAGAACTATCGGAGCTTCTGTATGTAATTCAAGAGAAAGAATGGTTCCCTTCTTTTCAATCTGCTTTACCATTAGC
TGGACATCCTGAAAGAATGGTTGGTGGAACATTGAGGAATCGGATGACAAATAGTCCTGCAACGGAAAATGCAATGGCAA
AAACGGGATCGATTACAGGAGTTTCTACATTATCCGGTTATGTAACAACGGCTGATGGAAAGGAAGTTGTTTTCTCTATA
CTCATCAATAACTATCTTGGATCAAGCTCTACAATGAGGGGTATTGAAGACAGTATTGTAGATACGATAGCAGGTCATGA
ATTTTAA

Upstream 100 bases:

>100_bases
TATTTCTAGTTCTAAAGAATACATTTACTAAGAAAAGCTATTTGTAAATTGGAAAATGTTGTATAATTATTAGAATATTA
AAAAAGAGAGGTGTGTGTCC

Downstream 100 bases:

>100_bases
AATAATATATTAAGATTGTTACTGAGCAAGTGGAATAGTAGCCTTTTAAGGATACATATTTCATTTGCTTTTTTTTTGAG
TCATTACATTAGTCCATTTC

Product: D-alanyl-D-alanine carboxypeptidase

Products: NA

Alternate protein names: DD-carboxypeptidase; DD-peptidase; Penicillin-binding protein 4a; PBP-4a [H]

Number of amino acids: Translated: 508; Mature: 508

Protein sequence:

>508_residues
MNRKAFVSKLVVLVGLIAIISGLLLFYPHLETKIDATDETDITQLEDATLEEKLAVILDNPNLEGGITGVSVMDAANGDQ
LFSRNIDIRLHPASNMKVLTAVSALETLGPDHRFTTEVLTDGKIRGKVLHGDVYVKGKGDPTLLQSDLEAFAKELKKQGI
HKIKGDLVGDDTWYDDVRLSQDLNWSDEPFFTGAQVSALTMSPDDDYDAGTVIVEVRPSENGKKAEVTLTPENNYISIEN
KTKMVANGEEKDISIERQHGNNTIVIEGEMPKDGSLSRSWASVWEPTNYVISVFRQTLEEEGITFIGKSKDRFEETPSSA
EIRVAKESMPLKELLIPFMKLSNNGHGETLVKEMGKVVEDDGSWDEGLVVMEDVLGELGLNMDTILLRDGSGMSHKNFIP
AVELSELLYVIQEKEWFPSFQSALPLAGHPERMVGGTLRNRMTNSPATENAMAKTGSITGVSTLSGYVTTADGKEVVFSI
LINNYLGSSSTMRGIEDSIVDTIAGHEF

Sequences:

>Translated_508_residues
MNRKAFVSKLVVLVGLIAIISGLLLFYPHLETKIDATDETDITQLEDATLEEKLAVILDNPNLEGGITGVSVMDAANGDQ
LFSRNIDIRLHPASNMKVLTAVSALETLGPDHRFTTEVLTDGKIRGKVLHGDVYVKGKGDPTLLQSDLEAFAKELKKQGI
HKIKGDLVGDDTWYDDVRLSQDLNWSDEPFFTGAQVSALTMSPDDDYDAGTVIVEVRPSENGKKAEVTLTPENNYISIEN
KTKMVANGEEKDISIERQHGNNTIVIEGEMPKDGSLSRSWASVWEPTNYVISVFRQTLEEEGITFIGKSKDRFEETPSSA
EIRVAKESMPLKELLIPFMKLSNNGHGETLVKEMGKVVEDDGSWDEGLVVMEDVLGELGLNMDTILLRDGSGMSHKNFIP
AVELSELLYVIQEKEWFPSFQSALPLAGHPERMVGGTLRNRMTNSPATENAMAKTGSITGVSTLSGYVTTADGKEVVFSI
LINNYLGSSSTMRGIEDSIVDTIAGHEF
>Mature_508_residues
MNRKAFVSKLVVLVGLIAIISGLLLFYPHLETKIDATDETDITQLEDATLEEKLAVILDNPNLEGGITGVSVMDAANGDQ
LFSRNIDIRLHPASNMKVLTAVSALETLGPDHRFTTEVLTDGKIRGKVLHGDVYVKGKGDPTLLQSDLEAFAKELKKQGI
HKIKGDLVGDDTWYDDVRLSQDLNWSDEPFFTGAQVSALTMSPDDDYDAGTVIVEVRPSENGKKAEVTLTPENNYISIEN
KTKMVANGEEKDISIERQHGNNTIVIEGEMPKDGSLSRSWASVWEPTNYVISVFRQTLEEEGITFIGKSKDRFEETPSSA
EIRVAKESMPLKELLIPFMKLSNNGHGETLVKEMGKVVEDDGSWDEGLVVMEDVLGELGLNMDTILLRDGSGMSHKNFIP
AVELSELLYVIQEKEWFPSFQSALPLAGHPERMVGGTLRNRMTNSPATENAMAKTGSITGVSTLSGYVTTADGKEVVFSI
LINNYLGSSSTMRGIEDSIVDTIAGHEF

Specific function: Catalyzes DD-carboxypeptidase and transpeptidation reactions [H]

COG id: COG2027

COG function: function code M; D-alanyl-D-alanine carboxypeptidase (penicillin-binding protein 4)

Gene ontology:

Cell location: Secreted (Probable). Note=May be anchored in the membrane via a C-terminal amphiphatic alpha helix [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the peptidase S13 family [H]

Homologues:

Organism=Escherichia coli, GI1789573, Length=401, Percent_Identity=27.1820448877805, Blast_Score=138, Evalue=7e-34,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR012338
- InterPro:   IPR000667 [H]

Pfam domain/function: PF02113 Peptidase_S13 [H]

EC number: =3.4.16.4 [H]

Molecular weight: Translated: 55714; Mature: 55714

Theoretical pI: Translated: 4.42; Mature: 4.42

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.0 %Cys     (Translated Protein)
3.1 %Met     (Translated Protein)
3.1 %Cys+Met (Translated Protein)
0.0 %Cys     (Mature Protein)
3.1 %Met     (Mature Protein)
3.1 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MNRKAFVSKLVVLVGLIAIISGLLLFYPHLETKIDATDETDITQLEDATLEEKLAVILDN
CCHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCEECCCCCCCCHHHHHHHHCCCEEEEEEC
PNLEGGITGVSVMDAANGDQLFSRNIDIRLHPASNMKVLTAVSALETLGPDHRFTTEVLT
CCCCCCCCCEEEEECCCCCHHHHCCCCEEEECCCCCCHHHHHHHHHHCCCCCCEEHHHHC
DGKIRGKVLHGDVYVKGKGDPTLLQSDLEAFAKELKKQGIHKIKGDLVGDDTWYDDVRLS
CCCEEEEEEEEEEEEECCCCHHHHHHHHHHHHHHHHHCCHHHHCCCCCCCCCCCCCEEEC
QDLNWSDEPFFTGAQVSALTMSPDDDYDAGTVIVEVRPSENGKKAEVTLTPENNYISIEN
CCCCCCCCCCCCCCEEEEEEECCCCCCCCCEEEEEEECCCCCCEEEEEECCCCCEEEECC
KTKMVANGEEKDISIERQHGNNTIVIEGEMPKDGSLSRSWASVWEPTNYVISVFRQTLEE
CEEEEECCCCCCCEEEEECCCCEEEEECCCCCCCCCCCHHHHHCCCHHHHHHHHHHHHHH
EGITFIGKSKDRFEETPSSAEIRVAKESMPLKELLIPFMKLSNNGHGETLVKEMGKVVED
CCCEEEECCHHHHHCCCCCCEEEEEHHCCCHHHHHHHHHHHCCCCCCHHHHHHHHHHHCC
DGSWDEGLVVMEDVLGELGLNMDTILLRDGSGMSHKNFIPAVELSELLYVIQEKEWFPSF
CCCCCCCHHHHHHHHHHHCCCEEEEEEECCCCCCCCCCCCHHHHHHHHHHHHHHHCCCCH
QSALPLAGHPERMVGGTLRNRMTNSPATENAMAKTGSITGVSTLSGYVTTADGKEVVFSI
HHCCCCCCCCHHHHHHHHHHHCCCCCCCCCHHHHCCCCCEEHHCCCEEEECCCHHHHHHH
LINNYLGSSSTMRGIEDSIVDTIAGHEF
HHHHHCCCCHHHCCHHHHHHHHHCCCCC
>Mature Secondary Structure
MNRKAFVSKLVVLVGLIAIISGLLLFYPHLETKIDATDETDITQLEDATLEEKLAVILDN
CCHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCEECCCCCCCCHHHHHHHHCCCEEEEEEC
PNLEGGITGVSVMDAANGDQLFSRNIDIRLHPASNMKVLTAVSALETLGPDHRFTTEVLT
CCCCCCCCCEEEEECCCCCHHHHCCCCEEEECCCCCCHHHHHHHHHHCCCCCCEEHHHHC
DGKIRGKVLHGDVYVKGKGDPTLLQSDLEAFAKELKKQGIHKIKGDLVGDDTWYDDVRLS
CCCEEEEEEEEEEEEECCCCHHHHHHHHHHHHHHHHHCCHHHHCCCCCCCCCCCCCEEEC
QDLNWSDEPFFTGAQVSALTMSPDDDYDAGTVIVEVRPSENGKKAEVTLTPENNYISIEN
CCCCCCCCCCCCCCEEEEEEECCCCCCCCCEEEEEEECCCCCCEEEEEECCCCCEEEECC
KTKMVANGEEKDISIERQHGNNTIVIEGEMPKDGSLSRSWASVWEPTNYVISVFRQTLEE
CEEEEECCCCCCCEEEEECCCCEEEEECCCCCCCCCCCHHHHHCCCHHHHHHHHHHHHHH
EGITFIGKSKDRFEETPSSAEIRVAKESMPLKELLIPFMKLSNNGHGETLVKEMGKVVED
CCCEEEECCHHHHHCCCCCCEEEEEHHCCCHHHHHHHHHHHCCCCCCHHHHHHHHHHHCC
DGSWDEGLVVMEDVLGELGLNMDTILLRDGSGMSHKNFIPAVELSELLYVIQEKEWFPSF
CCCCCCCHHHHHHHHHHHCCCEEEEEEECCCCCCCCCCCCHHHHHHHHHHHHHHHCCCCH
QSALPLAGHPERMVGGTLRNRMTNSPATENAMAKTGSITGVSTLSGYVTTADGKEVVFSI
HHCCCCCCCCHHHHHHHHHHHCCCCCCCCCHHHHCCCCCEEHHCCCEEEECCCHHHHHHH
LINNYLGSSSTMRGIEDSIVDTIAGHEF
HHHHHCCCCHHHCCHHHHHHHHHCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 6.0

TargetDB status: NA

Availability: NA

References: 7711903; 9384377 [H]