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The map label for this gene is yfkO [H]

Identifier: 23100581

GI number: 23100581

Start: 3259373

End: 3260041

Strand: Reverse

Name: yfkO [H]

Synonym: OB3126

Alternate gene names: 23100581

Gene position: 3260041-3259373 (Counterclockwise)

Preceding gene: 23100582

Following gene: 23100578

Centisome position: 89.8

GC content: 36.92

Gene sequence:

>669_bases
ATGACGGATAAGGCGAAAAAGAAACAAGAGATTTTAGAGGCTTTTCAATTTAGACATGCTACAAAAGAATACGATGGTAG
CAAGAAGATTGAAGACGAAGATTTTAAATTTATATTGGAAACAGGTAGGTTATCACCAAGCTCACTCGGTCTTGAACCTT
GGAAATTTTTAATCGTGCAAAGTTCAGATTTAAGAGAGAAATTAATGCAACTTGCTTCAGGAGCGGAACGACAACTTAGT
GGAGCGAGTCATTTTGTAATTATTCTTGCGCGAGTAGATGTTCGATATGATTCCGACTATGTGGCTAATCATTTAAGAAC
AGTTCAACAAATGCCTGATGAAGTTGCTGATATGCTTTTAAAAGGGATAAAGACATTTCAAGAAAACTTCTTGCAGTTAT
TTAATGATCGTACTTTATTTGATTGGTCATCAAAACAGACATATATCGCATTAGCGAATATGATGACAGCTGCTGCCCAG
ATTGGAATTGATTCTACACCAATAGAAGGATTCATCTATAAGGATGTGCATAACCTACTTGAAAGTGAAGGTTTACTAGA
AGACGGACGATATCAGCCTTCCGTCATGGCGGCTTTTGGATATCGTAAGGAAGAACCGAGCCGACCGAAAACAAGAAAAA
ATTTAAATGATATTGTGGAATGGGTGTAA

Upstream 100 bases:

>100_bases
TATAGTTTTTTTATTATAGTTGGCAACGAAGTGTACAATTATTTGTTCACAATTAGATGAAATGATAATATGTACGACAT
GCTTAGGAAAGGGGTATTCT

Downstream 100 bases:

>100_bases
TTCAAACGATAAAAAAAACAAACAAAAAGCAAAACAGCGTGTGAGTGGACTCGTCTGTTTTGCTTTTTGTTAAGGGCTGT
CAAGAACCTACATCCACCAG

Product: NAD(P)H nitroreductase

Products: NAD(P)(+); @TETR01.txt*5,6,7,8-Tetrahydropteridine!. [C]

Alternate protein names: NA

Number of amino acids: Translated: 222; Mature: 221

Protein sequence:

>222_residues
MTDKAKKKQEILEAFQFRHATKEYDGSKKIEDEDFKFILETGRLSPSSLGLEPWKFLIVQSSDLREKLMQLASGAERQLS
GASHFVIILARVDVRYDSDYVANHLRTVQQMPDEVADMLLKGIKTFQENFLQLFNDRTLFDWSSKQTYIALANMMTAAAQ
IGIDSTPIEGFIYKDVHNLLESEGLLEDGRYQPSVMAAFGYRKEEPSRPKTRKNLNDIVEWV

Sequences:

>Translated_222_residues
MTDKAKKKQEILEAFQFRHATKEYDGSKKIEDEDFKFILETGRLSPSSLGLEPWKFLIVQSSDLREKLMQLASGAERQLS
GASHFVIILARVDVRYDSDYVANHLRTVQQMPDEVADMLLKGIKTFQENFLQLFNDRTLFDWSSKQTYIALANMMTAAAQ
IGIDSTPIEGFIYKDVHNLLESEGLLEDGRYQPSVMAAFGYRKEEPSRPKTRKNLNDIVEWV
>Mature_221_residues
TDKAKKKQEILEAFQFRHATKEYDGSKKIEDEDFKFILETGRLSPSSLGLEPWKFLIVQSSDLREKLMQLASGAERQLSG
ASHFVIILARVDVRYDSDYVANHLRTVQQMPDEVADMLLKGIKTFQENFLQLFNDRTLFDWSSKQTYIALANMMTAAAQI
GIDSTPIEGFIYKDVHNLLESEGLLEDGRYQPSVMAAFGYRKEEPSRPKTRKNLNDIVEWV

Specific function: Reduction Of A Variety Of Nitroaromatic Compounds Using NADH (And To Lesser Extent Nadph) As Source Of Reducing Equivalents; Two Electrons Are Transferred. Capable Of Reducing Nitrofurazone, Quinones And The Anti-Tumor Agent Cb1954 (5- (Aziridin-1-Yl)-2,4

COG id: COG0778

COG function: function code C; Nitroreductase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the nitroreductase family [H]

Homologues:

Organism=Escherichia coli, GI1786792, Length=208, Percent_Identity=28.8461538461538, Blast_Score=76, Evalue=2e-15,

Paralogues:

None

Copy number: 260 Molecules/Cell In: Growth Phase, Minimal Media (Based on E. coli). 20 Molecules/Cell In: Stationary Phase, Rich Media (Based on E. coli). [C]

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR000415 [H]

Pfam domain/function: PF00881 Nitroreductase [H]

EC number: 1.-.-.- [C]

Molecular weight: Translated: 25520; Mature: 25389

Theoretical pI: Translated: 5.42; Mature: 5.42

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.0 %Cys     (Translated Protein)
3.2 %Met     (Translated Protein)
3.2 %Cys+Met (Translated Protein)
0.0 %Cys     (Mature Protein)
2.7 %Met     (Mature Protein)
2.7 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MTDKAKKKQEILEAFQFRHATKEYDGSKKIEDEDFKFILETGRLSPSSLGLEPWKFLIVQ
CCCHHHHHHHHHHHHHHHHHHHHCCCCCCCCCHHHHHHHHHCCCCCCCCCCCCEEEEEEC
SSDLREKLMQLASGAERQLSGASHFVIILARVDVRYDSDYVANHLRTVQQMPDEVADMLL
CHHHHHHHHHHHCCHHHHHCCCCEEEEEEEEECCCCCHHHHHHHHHHHHHCHHHHHHHHH
KGIKTFQENFLQLFNDRTLFDWSSKQTYIALANMMTAAAQIGIDSTPIEGFIYKDVHNLL
HHHHHHHHHHHHHHCCCCEEECCCCCHHHHHHHHHHHHHHHCCCCCCCCHHHHHHHHHHH
ESEGLLEDGRYQPSVMAAFGYRKEEPSRPKTRKNLNDIVEWV
HHCCCCCCCCCCCHHHHHHCCCCCCCCCCHHHHHHHHHHHCC
>Mature Secondary Structure 
TDKAKKKQEILEAFQFRHATKEYDGSKKIEDEDFKFILETGRLSPSSLGLEPWKFLIVQ
CCHHHHHHHHHHHHHHHHHHHHCCCCCCCCCHHHHHHHHHCCCCCCCCCCCCEEEEEEC
SSDLREKLMQLASGAERQLSGASHFVIILARVDVRYDSDYVANHLRTVQQMPDEVADMLL
CHHHHHHHHHHHCCHHHHHCCCCEEEEEEEEECCCCCHHHHHHHHHHHHHCHHHHHHHHH
KGIKTFQENFLQLFNDRTLFDWSSKQTYIALANMMTAAAQIGIDSTPIEGFIYKDVHNLL
HHHHHHHHHHHHHHCCCCEEECCCCCHHHHHHHHHHHHHHHCCCCCCCCHHHHHHHHHHH
ESEGLLEDGRYQPSVMAAFGYRKEEPSRPKTRKNLNDIVEWV
HHCCCCCCCCCCCHHHHHHCCCCCCCCCCHHHHHHHHHHHCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: FMN. [C]

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NAD(P)H; 6,7-Dihydropteridine [C]

Specific reaction: NAD(P)H + 6,7-Dihydropteridine = NAD(P)(+) + @TETR01.txt*5,6,7,8-Tetrahydropteridine!. [C]

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 9384377 [H]