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The map label for this gene is yugJ [H]

Identifier: 23100514

GI number: 23100514

Start: 3176390

End: 3177559

Strand: Reverse

Name: yugJ [H]

Synonym: OB3059

Alternate gene names: 23100514

Gene position: 3177559-3176390 (Counterclockwise)

Preceding gene: 23100515

Following gene: 23100512

Centisome position: 87.52

GC content: 37.86

Gene sequence:

>1170_bases
ATGGATAATTTTGTGTATCAAAATCCGACCAAACTAATTTTTGGTAAAGGACAGATTGAACAACTTTCAAAACAGGTTGA
ACCTTTTGGAAAACGTGTATTGATTGTTTATGGCGGAGGAAGTATAAAGCGTAATGGTATTTATGATAATGTTCTTGCCG
AACTTGGAAAAATAGAAGCAGCTGTATTTGAATTATCAGGTGTTGAGCCAAACCCACGAATTTCTACTGTTCGAAAAGGT
GTAGAAATTTGTCGTCAAGAATCCATTGATTTTATATTAGCAGTCGGTGGCGGCAGTACGATTGATTGTACGAAAGCAAT
TGCAGTAGGTGCAGTAACTGAGGCTGATATGTGGGATGTTGTTACGAAAAAAGAAAAGGCAGAAGGTGCTTTGCCGTTTG
GTACAATACTTACATTAGCAGCAACTGGTTCTGAAATGAATGCTGGATCGGTTATAACGAATTGGGAAACCAATGAAAAA
CATGGTTGGGGCTCTCCATACTCATTTCCGAAGTTTTCTATATTAGACCCTGTTCATACATTTTCTGTGCCTCGTGATCA
AACGATATACGGTATTGTTGATATGATGTCTCACGTATTAGAGCATTATTTCCATCATACAACAAATACCCCAATACAAG
ACCGCTGGTGCGAAGGGATATTATTAACAATGATAGATACTGGACCTAAACTATTAGACGATCCAGAAAACTATGAGTAT
CGATCAACAGCTATGTTAGGTGGAACTTTAGCATTAAATAACATGCTGAATTTAGGCTATCATGGTGACTGGGCAACCCA
TAATTTAGAGCATGCGGTTTCAGCTGTGCATGATATTCCGCATGGGGGTGGTTTGGCAATCTTATTCCCTCAGTGGATGC
GACATGTATTAGATGAAGAGAATGCACCACGCTTTAAACAATTAGCAGTAAGAGTATTTGATGTAGCTGAACAAGGTAAG
TCAGATAAAGAAGTTGCACTTGAAGGTATTGATAAGCTTCAAGAATTTTGGTCAAGCATTGGCGCTCCAACTAAGTTAAG
TGATTATGATATTAATGAAGGTTCTGTCGAAGAAATGGCAGATAAAACGGTAATCGCTAGACCAGAATTTGGTAATTATA
AAAAGCTAACTAGACAAGATTCTGTCGATATTTATACGGCAAGTTTGTAA

Upstream 100 bases:

>100_bases
AGCAAGAGCAACAGTTGAAGTTAGTCGTTTGCCGAAGGACGTATTAATTGAAATGGACCTTGTTGCATACGTGAAATAGG
TAAAGTAGAGGAGGAATAGT

Downstream 100 bases:

>100_bases
TTTCCCGGGAAATAAATTGTCGTATTAAAAAAGAGCTTGTCGTGTATTATTCGTACACGGCAAGCTCTTCTTTAATTTGC
TTACGTATTTTATCTAAACA

Product: NADH-dependent butanol dehydrogenase

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 389; Mature: 389

Protein sequence:

>389_residues
MDNFVYQNPTKLIFGKGQIEQLSKQVEPFGKRVLIVYGGGSIKRNGIYDNVLAELGKIEAAVFELSGVEPNPRISTVRKG
VEICRQESIDFILAVGGGSTIDCTKAIAVGAVTEADMWDVVTKKEKAEGALPFGTILTLAATGSEMNAGSVITNWETNEK
HGWGSPYSFPKFSILDPVHTFSVPRDQTIYGIVDMMSHVLEHYFHHTTNTPIQDRWCEGILLTMIDTGPKLLDDPENYEY
RSTAMLGGTLALNNMLNLGYHGDWATHNLEHAVSAVHDIPHGGGLAILFPQWMRHVLDEENAPRFKQLAVRVFDVAEQGK
SDKEVALEGIDKLQEFWSSIGAPTKLSDYDINEGSVEEMADKTVIARPEFGNYKKLTRQDSVDIYTASL

Sequences:

>Translated_389_residues
MDNFVYQNPTKLIFGKGQIEQLSKQVEPFGKRVLIVYGGGSIKRNGIYDNVLAELGKIEAAVFELSGVEPNPRISTVRKG
VEICRQESIDFILAVGGGSTIDCTKAIAVGAVTEADMWDVVTKKEKAEGALPFGTILTLAATGSEMNAGSVITNWETNEK
HGWGSPYSFPKFSILDPVHTFSVPRDQTIYGIVDMMSHVLEHYFHHTTNTPIQDRWCEGILLTMIDTGPKLLDDPENYEY
RSTAMLGGTLALNNMLNLGYHGDWATHNLEHAVSAVHDIPHGGGLAILFPQWMRHVLDEENAPRFKQLAVRVFDVAEQGK
SDKEVALEGIDKLQEFWSSIGAPTKLSDYDINEGSVEEMADKTVIARPEFGNYKKLTRQDSVDIYTASL
>Mature_389_residues
MDNFVYQNPTKLIFGKGQIEQLSKQVEPFGKRVLIVYGGGSIKRNGIYDNVLAELGKIEAAVFELSGVEPNPRISTVRKG
VEICRQESIDFILAVGGGSTIDCTKAIAVGAVTEADMWDVVTKKEKAEGALPFGTILTLAATGSEMNAGSVITNWETNEK
HGWGSPYSFPKFSILDPVHTFSVPRDQTIYGIVDMMSHVLEHYFHHTTNTPIQDRWCEGILLTMIDTGPKLLDDPENYEY
RSTAMLGGTLALNNMLNLGYHGDWATHNLEHAVSAVHDIPHGGGLAILFPQWMRHVLDEENAPRFKQLAVRVFDVAEQGK
SDKEVALEGIDKLQEFWSSIGAPTKLSDYDINEGSVEEMADKTVIARPEFGNYKKLTRQDSVDIYTASL

Specific function: Unknown

COG id: COG1979

COG function: function code C; Uncharacterized oxidoreductases, Fe-dependent alcohol dehydrogenase family

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the iron-containing alcohol dehydrogenase family [H]

Homologues:

Organism=Escherichia coli, GI1789386, Length=365, Percent_Identity=41.0958904109589, Blast_Score=277, Evalue=7e-76,
Organism=Escherichia coli, GI48994951, Length=358, Percent_Identity=27.6536312849162, Blast_Score=115, Evalue=3e-27,
Organism=Escherichia coli, GI1789163, Length=387, Percent_Identity=23.2558139534884, Blast_Score=100, Evalue=2e-22,
Organism=Escherichia coli, GI87082107, Length=346, Percent_Identity=23.9884393063584, Blast_Score=83, Evalue=3e-17,
Organism=Escherichia coli, GI1787493, Length=344, Percent_Identity=23.546511627907, Blast_Score=73, Evalue=3e-14,
Organism=Caenorhabditis elegans, GI17537053, Length=292, Percent_Identity=26.027397260274, Blast_Score=88, Evalue=6e-18,
Organism=Saccharomyces cerevisiae, GI6321181, Length=384, Percent_Identity=25.78125, Blast_Score=100, Evalue=7e-22,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR001670
- InterPro:   IPR018211 [H]

Pfam domain/function: PF00465 Fe-ADH [H]

EC number: 1.1.-.- [C]

Molecular weight: Translated: 43037; Mature: 43037

Theoretical pI: Translated: 4.89; Mature: 4.89

Prosite motif: PS00913 ADH_IRON_1 ; PS00060 ADH_IRON_2

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.8 %Cys     (Translated Protein)
2.6 %Met     (Translated Protein)
3.3 %Cys+Met (Translated Protein)
0.8 %Cys     (Mature Protein)
2.6 %Met     (Mature Protein)
3.3 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MDNFVYQNPTKLIFGKGQIEQLSKQVEPFGKRVLIVYGGGSIKRNGIYDNVLAELGKIEA
CCCCCCCCCCEEEECCCHHHHHHHHHCCCCCEEEEEECCCCEECCCCHHHHHHHHHHHHE
AVFELSGVEPNPRISTVRKGVEICRQESIDFILAVGGGSTIDCTKAIAVGAVTEADMWDV
EEEEECCCCCCCHHHHHHHHHHHHHHCCCCEEEEECCCCCCHHHHHHHHCCCCCCHHHHH
VTKKEKAEGALPFGTILTLAATGSEMNAGSVITNWETNEKHGWGSPYSFPKFSILDPVHT
HHHHHHCCCCCCHHHHHEEEECCCCCCCCCEEECCCCCCCCCCCCCCCCCCCCCCCCHHH
FSVPRDQTIYGIVDMMSHVLEHYFHHTTNTPIQDRWCEGILLTMIDTGPKLLDDPENYEY
CCCCCCCHHHHHHHHHHHHHHHHHHCCCCCCHHHHHHCCEEEEEECCCCCCCCCCCCCCC
RSTAMLGGTLALNNMLNLGYHGDWATHNLEHAVSAVHDIPHGGGLAILFPQWMRHVLDEE
HHHHHCCCHHHHHHHHHCCCCCCCHHCHHHHHHHHHHHCCCCCCEEEEHHHHHHHHHCCC
NAPRFKQLAVRVFDVAEQGKSDKEVALEGIDKLQEFWSSIGAPTKLSDYDINEGSVEEMA
CCCHHHHHHHHHHHHHHCCCCCHHHHHHHHHHHHHHHHHHCCCCCCCCCCCCCCCHHHHH
DKTVIARPEFGNYKKLTRQDSVDIYTASL
CCEEEECCCCCCHHHHHCCCCCCEEEECC
>Mature Secondary Structure
MDNFVYQNPTKLIFGKGQIEQLSKQVEPFGKRVLIVYGGGSIKRNGIYDNVLAELGKIEA
CCCCCCCCCCEEEECCCHHHHHHHHHCCCCCEEEEEECCCCEECCCCHHHHHHHHHHHHE
AVFELSGVEPNPRISTVRKGVEICRQESIDFILAVGGGSTIDCTKAIAVGAVTEADMWDV
EEEEECCCCCCCHHHHHHHHHHHHHHCCCCEEEEECCCCCCHHHHHHHHCCCCCCHHHHH
VTKKEKAEGALPFGTILTLAATGSEMNAGSVITNWETNEKHGWGSPYSFPKFSILDPVHT
HHHHHHCCCCCCHHHHHEEEECCCCCCCCCEEECCCCCCCCCCCCCCCCCCCCCCCCHHH
FSVPRDQTIYGIVDMMSHVLEHYFHHTTNTPIQDRWCEGILLTMIDTGPKLLDDPENYEY
CCCCCCCHHHHHHHHHHHHHHHHHHCCCCCCHHHHHHCCEEEEEECCCCCCCCCCCCCCC
RSTAMLGGTLALNNMLNLGYHGDWATHNLEHAVSAVHDIPHGGGLAILFPQWMRHVLDEE
HHHHHCCCHHHHHHHHHCCCCCCCHHCHHHHHHHHHHHCCCCCCEEEEHHHHHHHHHCCC
NAPRFKQLAVRVFDVAEQGKSDKEVALEGIDKLQEFWSSIGAPTKLSDYDINEGSVEEMA
CCCHHHHHHHHHHHHHHCCCCCHHHHHHHHHHHHHHHHHHCCCCCCCCCCCCCCCHHHHH
DKTVIARPEFGNYKKLTRQDSVDIYTASL
CCEEEECCCCCCHHHHHCCCCCCEEEECC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 9274030; 9384377 [H]