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The map label for this gene is kbl [C]

Identifier: 23100509

GI number: 23100509

Start: 3170281

End: 3171471

Strand: Reverse

Name: kbl [C]

Synonym: OB3054

Alternate gene names: 23100509

Gene position: 3171471-3170281 (Counterclockwise)

Preceding gene: 23100511

Following gene: 23100508

Centisome position: 87.36

GC content: 35.68

Gene sequence:

>1191_bases
ATGACAAGCAGTAAATTAGATCAATTTTTACAAGAAAACATTTCAGATTTGAAAGATCGCGGACTATATAATGAAATTGA
TACAGTACAGGGTGCAAATGGACCTGAGATAACAATTGAAGGAAAAAAACTAATCAACCTATCTTCAAATAATTATCTAG
GACTTGCAACCAATAATGAAATGAAAGAAATAGCAAAAAAAGCAATAGATTCTCACGGTGTCGGAGCAGGGGCTGTAAGA
ACCATTAATGGTACTTTAGATTTACATATTGAGTTAGAAAAGAAAATTGCTCAATTCAAAGGAACAGAAGCAGCAATTGC
TTACCAATCTGGCTTTAACTGTAATATGGCGGCAATTTCTGCTGTTATGGATAAAAATGATGCCATTTTGTCTGATTCAC
TTAATCATGCATCTATTATAGATGGCTGTCGTTTATCAAAGGCGAAAATCATTCGATTTGAACATTCTGATATGAATGAT
TTACGTCAGAAAGCGAAAGAAGCAGTAGAGTCCGGTCAATATAATAAGATTATGGTAATTACAGATGGGGTATTCTCAAT
GGATGGGGATATTGCAAAACTTCCTGAGATTGTAGACATTGCGGAAGAATTTGACTTGATTACGTATGTAGATGATGCGC
ATGGATCCGGTGTTACTGGAGATGGTGCAGGTACGGTTAAACATTTTGGCTTGCAAGATAAAGTAGATATGCAAATGGGA
ACTTTATCAAAAGCAATTGGAGTTATCGGTGGATATGTAGCTGGAAAGCAATCATTAATTGATTGGCTAAAAGTACGTTC
ACGTCCATTCTTATTCTCTACGGCAGTATCCCCAGCGGATGCAGTTGCAAGTAAAAGAGCAATTGAAATGTTAATGGAAA
GCACTGAACTAAACGAAAAATTATGGGAAAACGGTGACTACCTAAAACAAGGTCTTAAAGAATTAGGTTTTGATATTGGG
GAAAGTGAAACTCCAATTACTCCTTGTATTATTGGAGATGAGAAAAAAGCACAAGAATTTAGTAAGCGTCTATATGAGGA
AGGCGTATATGCAAAATCTATCGTATTTCCAACTGTACCAAGAGGAACCGGTAGAGTAAGAAATATGCCTACTGCAGCGC
ATACGAAAGAAATGCTTGATGATGCAATTGCCATTTACAAAAAAATTGGACAAGAAATGAAACTTATATAA

Upstream 100 bases:

>100_bases
ATAGACAGTAACTATAGGGCATAATTAAACATTTTAATCGGTTTATGGTAAACTTAGAGAGGTTGATAAATTCTCTCACT
CTTAAGGAGGATGATTTAGT

Downstream 100 bases:

>100_bases
TGAATGCCAAGTAAGAGGAGATTTTTACTATGAAAAAGATTTTAGTTACAGGAGCCTTAGGACAAATCGGCTCCGAGTTA
ACAGGTATGCTCCGTCGAAT

Product: 2-amino-3-ketobutyrate coenzyme A ligase

Products: NA

Alternate protein names: AONS/AKB ligase; 7-keto-8-amino-pelargonic acid synthase; 7-KAP synthase; KAPA synthase; 8-amino-7-ketopelargonate synthase; Alpha-oxoamine synthase; L-alanine--pimeloyl-CoA ligase

Number of amino acids: Translated: 396; Mature: 395

Protein sequence:

>396_residues
MTSSKLDQFLQENISDLKDRGLYNEIDTVQGANGPEITIEGKKLINLSSNNYLGLATNNEMKEIAKKAIDSHGVGAGAVR
TINGTLDLHIELEKKIAQFKGTEAAIAYQSGFNCNMAAISAVMDKNDAILSDSLNHASIIDGCRLSKAKIIRFEHSDMND
LRQKAKEAVESGQYNKIMVITDGVFSMDGDIAKLPEIVDIAEEFDLITYVDDAHGSGVTGDGAGTVKHFGLQDKVDMQMG
TLSKAIGVIGGYVAGKQSLIDWLKVRSRPFLFSTAVSPADAVASKRAIEMLMESTELNEKLWENGDYLKQGLKELGFDIG
ESETPITPCIIGDEKKAQEFSKRLYEEGVYAKSIVFPTVPRGTGRVRNMPTAAHTKEMLDDAIAIYKKIGQEMKLI

Sequences:

>Translated_396_residues
MTSSKLDQFLQENISDLKDRGLYNEIDTVQGANGPEITIEGKKLINLSSNNYLGLATNNEMKEIAKKAIDSHGVGAGAVR
TINGTLDLHIELEKKIAQFKGTEAAIAYQSGFNCNMAAISAVMDKNDAILSDSLNHASIIDGCRLSKAKIIRFEHSDMND
LRQKAKEAVESGQYNKIMVITDGVFSMDGDIAKLPEIVDIAEEFDLITYVDDAHGSGVTGDGAGTVKHFGLQDKVDMQMG
TLSKAIGVIGGYVAGKQSLIDWLKVRSRPFLFSTAVSPADAVASKRAIEMLMESTELNEKLWENGDYLKQGLKELGFDIG
ESETPITPCIIGDEKKAQEFSKRLYEEGVYAKSIVFPTVPRGTGRVRNMPTAAHTKEMLDDAIAIYKKIGQEMKLI
>Mature_395_residues
TSSKLDQFLQENISDLKDRGLYNEIDTVQGANGPEITIEGKKLINLSSNNYLGLATNNEMKEIAKKAIDSHGVGAGAVRT
INGTLDLHIELEKKIAQFKGTEAAIAYQSGFNCNMAAISAVMDKNDAILSDSLNHASIIDGCRLSKAKIIRFEHSDMNDL
RQKAKEAVESGQYNKIMVITDGVFSMDGDIAKLPEIVDIAEEFDLITYVDDAHGSGVTGDGAGTVKHFGLQDKVDMQMGT
LSKAIGVIGGYVAGKQSLIDWLKVRSRPFLFSTAVSPADAVASKRAIEMLMESTELNEKLWENGDYLKQGLKELGFDIGE
SETPITPCIIGDEKKAQEFSKRLYEEGVYAKSIVFPTVPRGTGRVRNMPTAAHTKEMLDDAIAIYKKIGQEMKLI

Specific function: Catalyzes the decarboxylative condensation of pimeloyl- CoA and L-alanine to produce 8-amino-7-oxononanoate (AON), coenzyme A and/or converts 2-amino-3-ketobutyrate to glycine and acetyl-CoA

COG id: COG0156

COG function: function code H; 7-keto-8-aminopelargonate synthetase and related enzymes

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the class-II pyridoxal-phosphate-dependent aminotransferase family

Homologues:

Organism=Homo sapiens, GI7657118, Length=393, Percent_Identity=34.3511450381679, Blast_Score=254, Evalue=8e-68,
Organism=Homo sapiens, GI284448556, Length=351, Percent_Identity=36.4672364672365, Blast_Score=246, Evalue=3e-65,
Organism=Homo sapiens, GI4758668, Length=371, Percent_Identity=37.1967654986523, Blast_Score=238, Evalue=6e-63,
Organism=Homo sapiens, GI119220554, Length=370, Percent_Identity=34.3243243243243, Blast_Score=225, Evalue=6e-59,
Organism=Homo sapiens, GI4502025, Length=415, Percent_Identity=30.8433734939759, Blast_Score=191, Evalue=1e-48,
Organism=Homo sapiens, GI40316939, Length=415, Percent_Identity=30.8433734939759, Blast_Score=191, Evalue=1e-48,
Organism=Homo sapiens, GI83977442, Length=392, Percent_Identity=29.0816326530612, Blast_Score=187, Evalue=1e-47,
Organism=Homo sapiens, GI83977440, Length=392, Percent_Identity=29.0816326530612, Blast_Score=187, Evalue=1e-47,
Organism=Homo sapiens, GI83977444, Length=392, Percent_Identity=29.0816326530612, Blast_Score=187, Evalue=1e-47,
Organism=Homo sapiens, GI5454084, Length=393, Percent_Identity=27.4809160305344, Blast_Score=142, Evalue=5e-34,
Organism=Escherichia coli, GI1790046, Length=395, Percent_Identity=40.253164556962, Blast_Score=317, Evalue=9e-88,
Organism=Escherichia coli, GI1786993, Length=355, Percent_Identity=33.8028169014084, Blast_Score=188, Evalue=6e-49,
Organism=Caenorhabditis elegans, GI71994529, Length=393, Percent_Identity=38.9312977099237, Blast_Score=285, Evalue=2e-77,
Organism=Caenorhabditis elegans, GI32566772, Length=366, Percent_Identity=32.2404371584699, Blast_Score=207, Evalue=7e-54,
Organism=Caenorhabditis elegans, GI17560914, Length=363, Percent_Identity=32.7823691460055, Blast_Score=200, Evalue=1e-51,
Organism=Caenorhabditis elegans, GI17560912, Length=363, Percent_Identity=32.7823691460055, Blast_Score=200, Evalue=1e-51,
Organism=Caenorhabditis elegans, GI71982625, Length=277, Percent_Identity=28.5198555956679, Blast_Score=144, Evalue=1e-34,
Organism=Caenorhabditis elegans, GI71982617, Length=277, Percent_Identity=28.5198555956679, Blast_Score=144, Evalue=1e-34,
Organism=Saccharomyces cerevisiae, GI6320267, Length=391, Percent_Identity=31.9693094629156, Blast_Score=201, Evalue=2e-52,
Organism=Saccharomyces cerevisiae, GI6320438, Length=382, Percent_Identity=31.151832460733, Blast_Score=189, Evalue=6e-49,
Organism=Saccharomyces cerevisiae, GI6323954, Length=260, Percent_Identity=30.3846153846154, Blast_Score=138, Evalue=2e-33,
Organism=Drosophila melanogaster, GI24662918, Length=397, Percent_Identity=38.0352644836272, Blast_Score=299, Evalue=3e-81,
Organism=Drosophila melanogaster, GI17137420, Length=360, Percent_Identity=36.1111111111111, Blast_Score=201, Evalue=5e-52,
Organism=Drosophila melanogaster, GI17136286, Length=370, Percent_Identity=31.6216216216216, Blast_Score=189, Evalue=4e-48,
Organism=Drosophila melanogaster, GI24653280, Length=292, Percent_Identity=28.4246575342466, Blast_Score=146, Evalue=2e-35,
Organism=Drosophila melanogaster, GI24653276, Length=292, Percent_Identity=28.4246575342466, Blast_Score=146, Evalue=2e-35,
Organism=Drosophila melanogaster, GI24653278, Length=292, Percent_Identity=28.4246575342466, Blast_Score=146, Evalue=2e-35,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): BIKB_OCEIH (Q8EM07)

Other databases:

- EMBL:   BA000028
- RefSeq:   NP_693976.1
- ProteinModelPortal:   Q8EM07
- SMR:   Q8EM07
- GeneID:   1017886
- GenomeReviews:   BA000028_GR
- KEGG:   oih:OB3054
- NMPDR:   fig|221109.1.peg.3056
- HOGENOM:   HBG649839
- OMA:   MELHVEL
- ProtClustDB:   PRK06939
- BioCyc:   OIHE221109:OB3054-MONOMER
- BRENDA:   2.3.1.29
- InterPro:   IPR001917
- InterPro:   IPR004839
- InterPro:   IPR010962
- InterPro:   IPR015424
- InterPro:   IPR015421
- InterPro:   IPR015422
- Gene3D:   G3DSA:3.40.640.10
- Gene3D:   G3DSA:3.90.1150.10
- PANTHER:   PTHR13693:SF15
- TIGRFAMs:   TIGR01825

Pfam domain/function: PF00155 Aminotran_1_2; SSF53383 PyrdxlP-dep_Trfase_major

EC number: =2.3.1.29; =2.3.1.47

Molecular weight: Translated: 43237; Mature: 43106

Theoretical pI: Translated: 5.02; Mature: 5.02

Prosite motif: PS00599 AA_TRANSFER_CLASS_2

Important sites: BINDING 136-136 BINDING 186-186 BINDING 358-358

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.8 %Cys     (Translated Protein)
3.5 %Met     (Translated Protein)
4.3 %Cys+Met (Translated Protein)
0.8 %Cys     (Mature Protein)
3.3 %Met     (Mature Protein)
4.1 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MTSSKLDQFLQENISDLKDRGLYNEIDTVQGANGPEITIEGKKLINLSSNNYLGLATNNE
CCHHHHHHHHHHHHHHHHHCCCHHHHHHCCCCCCCEEEEECCEEEEECCCCEEEEECCHH
MKEIAKKAIDSHGVGAGAVRTINGTLDLHIELEKKIAQFKGTEAAIAYQSGFNCNMAAIS
HHHHHHHHHHCCCCCCCEEEEECCEEEEEEEHHHHHHHHCCCCEEEEECCCCCCCHHHHH
AVMDKNDAILSDSLNHASIIDGCRLSKAKIIRFEHSDMNDLRQKAKEAVESGQYNKIMVI
HHHCCCCCHHHCCCCHHHHHHCCCCCCEEEEEEECCCHHHHHHHHHHHHHCCCCCEEEEE
TDGVFSMDGDIAKLPEIVDIAEEFDLITYVDDAHGSGVTGDGAGTVKHFGLQDKVDMQMG
ECCCCCCCCCHHHHHHHHHHHHHCCEEEEEECCCCCCCCCCCCCCHHHCCCCCHHHHHHH
TLSKAIGVIGGYVAGKQSLIDWLKVRSRPFLFSTAVSPADAVASKRAIEMLMESTELNEK
HHHHHHHHHHHHHHCHHHHHHHHHHCCCCEEEEECCCCHHHHHHHHHHHHHHHHHHHHHH
LWENGDYLKQGLKELGFDIGESETPITPCIIGDEKKAQEFSKRLYEEGVYAKSIVFPTVP
HHCCCHHHHHHHHHHCCCCCCCCCCCCCEEECCHHHHHHHHHHHHHCCCCCCEEECCCCC
RGTGRVRNMPTAAHTKEMLDDAIAIYKKIGQEMKLI
CCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHCCC
>Mature Secondary Structure 
TSSKLDQFLQENISDLKDRGLYNEIDTVQGANGPEITIEGKKLINLSSNNYLGLATNNE
CHHHHHHHHHHHHHHHHHCCCHHHHHHCCCCCCCEEEEECCEEEEECCCCEEEEECCHH
MKEIAKKAIDSHGVGAGAVRTINGTLDLHIELEKKIAQFKGTEAAIAYQSGFNCNMAAIS
HHHHHHHHHHCCCCCCCEEEEECCEEEEEEEHHHHHHHHCCCCEEEEECCCCCCCHHHHH
AVMDKNDAILSDSLNHASIIDGCRLSKAKIIRFEHSDMNDLRQKAKEAVESGQYNKIMVI
HHHCCCCCHHHCCCCHHHHHHCCCCCCEEEEEEECCCHHHHHHHHHHHHHCCCCCEEEEE
TDGVFSMDGDIAKLPEIVDIAEEFDLITYVDDAHGSGVTGDGAGTVKHFGLQDKVDMQMG
ECCCCCCCCCHHHHHHHHHHHHHCCEEEEEECCCCCCCCCCCCCCHHHCCCCCHHHHHHH
TLSKAIGVIGGYVAGKQSLIDWLKVRSRPFLFSTAVSPADAVASKRAIEMLMESTELNEK
HHHHHHHHHHHHHHCHHHHHHHHHHCCCCEEEEECCCCHHHHHHHHHHHHHHHHHHHHHH
LWENGDYLKQGLKELGFDIGESETPITPCIIGDEKKAQEFSKRLYEEGVYAKSIVFPTVP
HHCCCHHHHHHHHHHCCCCCCCCCCCCCEEECCHHHHHHHHHHHHHCCCCCCEEECCCCC
RGTGRVRNMPTAAHTKEMLDDAIAIYKKIGQEMKLI
CCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 12235376