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The map label for this gene is mdeA [H]

Identifier: 23100503

GI number: 23100503

Start: 3162285

End: 3163955

Strand: Direct

Name: mdeA [H]

Synonym: OB3048

Alternate gene names: 23100503

Gene position: 3162285-3163955 (Clockwise)

Preceding gene: 23100502

Following gene: 23100504

Centisome position: 87.1

GC content: 39.14

Gene sequence:

>1671_bases
ATGTCTAAATTTCAATCTTTGCAAGCAGAAACAATCTTACTTCATGGAGGACAGGAACCAGACCCATCAACTGGTTCACG
TGCAGTTCCAATTTATCAAACTACGTCCTATGTGTTCCGAGATACCGAACATGCACAAAATTTATTTGGTTTAGCAGAAC
CGGGAAATATTTATTCACGAATTATGAATCCAACTGTAGATGCTTTTGAACAGCGAATCACTCAGCTTGAAGATGGTATT
GGTGCAGTTGCTACCTCTTCTGGTATGGCGGCAATTACGTTAGCGATTCTTAATATTGCTGGTTCTGGAGATGAAATTGT
TGCTGACAGCAACTTATACGGTGGTACGTTCAATCTATTTGCTAACACCCTCCCTCGTTACGGAATTAATGTGAAGTTTG
TGGATGGAACAGATCCTCAAGCGATTGATGATGCTATTACGGATAAGACGAAAGCAGTTTTCGGTGAAATTATTACGAAT
CCTAGCCTCTATGTATTTGATATCGAAAAAGTTTCTGAAGTAGCACATAAGCATGGTGTACCGTTAATTATTGATAATAC
CTTTGCTACACCTTATGTAACAAAGCCATTAACTTGGGGTGCAGATATTGTTGTTCACTCTGCAACAAAATGGATTGGAG
GACATGGAACAACCATTGGTGGTGTCGTGGTTGATAGTGGTCGATTTGATTGGAATAACGATCGTTTCCAAGACTTTGCA
GAACCTGATGAAAGTTATAATGGATTAAAATACGTTGACCTTGGGGCAGCAGCATACATTACAAAACTTAGAGTGCAATT
ATTACGTGATATTGGAGCTTCACTAAGCCCACAAAATGCTTTTCTGCTATTGCAAGGATTGGAGACATTACACTTACGCA
TTAAACAACATACCGATAATGCATTGCAATTAGCGAAATATTTACAAGCACATCCTGCTGTCGAATGGGTGAACTATCCA
GGATTAGAAGAGCATCCATCTCATGAACTGGCAGGTAAATATTTGAAACAAAGCTTCGGATCTATTATCACTTTTGGAAT
ATCTGGTGGTCGAGAAGCGGGACGAACATTAATAGATAATGTTTCATTGTGGTCACATGTTGCAAATGTAGGAGATGCAA
AATCCCTTATTATTCATCCGGCATCTACCACGCATCAACAATTAAACGAAGATGACTTAGCATTAAGTGGCGTAACTGAA
GAATTAGTACGATTATCCGTAGGTCTGGAGTCCATCGATGATATTATCGCTGATCTTGACCAAGCAATTGAGAAAGCTAC
TGGTAAATCACATACAAATAAACCGACCCAAGAAGAAATTGCAGTACAATGGTTACTTCACTCTCCATTCGATCGAAGCA
ATGGAGTGAGAAAAAAAGTAATAGCTACACTTCAAATAGACCAAGATCAACTAGCACCACTTGAAACGCTAGGGTATGAA
GTAGTACCAGTGACATCGTTACGTGATCTAAATACAGAGGTTGATGCTATCTGGTTTGATGAGACAGTCCAATCTATCGA
GGGAGAAGTACCGACAGATACCATTGTATTTGCATCGGATAGACAGTCCACTGCATTAAAAGATGCTATTGTAATAACCA
ATCAATCTCCAGCAAAAATCGCACTACAACTTCGTAATGGTGAAGAGTCAAGACAACCAATTAAGATATAA

Upstream 100 bases:

>100_bases
GGTTCGGTTTTAAACAGACAAAGTCCTCTTCTTATGAAGGGGGCTTTTTTTAATCCTTCTCTTATTACTTTAAAAATAAT
AAATTCAAGGAGGAAACACG

Downstream 100 bases:

>100_bases
TAACAAACAAAATGAGTTTACATTTCATTTTATTTGGGTAATAAAATATATATAAATAAAGTAGCCTTCCATAATGAAGG
CTATTTTTTTTGAAAATAAC

Product: O-acetylhomoserine sulfhydrylase

Products: NA

Alternate protein names: L-methioninase [H]

Number of amino acids: Translated: 556; Mature: 555

Protein sequence:

>556_residues
MSKFQSLQAETILLHGGQEPDPSTGSRAVPIYQTTSYVFRDTEHAQNLFGLAEPGNIYSRIMNPTVDAFEQRITQLEDGI
GAVATSSGMAAITLAILNIAGSGDEIVADSNLYGGTFNLFANTLPRYGINVKFVDGTDPQAIDDAITDKTKAVFGEIITN
PSLYVFDIEKVSEVAHKHGVPLIIDNTFATPYVTKPLTWGADIVVHSATKWIGGHGTTIGGVVVDSGRFDWNNDRFQDFA
EPDESYNGLKYVDLGAAAYITKLRVQLLRDIGASLSPQNAFLLLQGLETLHLRIKQHTDNALQLAKYLQAHPAVEWVNYP
GLEEHPSHELAGKYLKQSFGSIITFGISGGREAGRTLIDNVSLWSHVANVGDAKSLIIHPASTTHQQLNEDDLALSGVTE
ELVRLSVGLESIDDIIADLDQAIEKATGKSHTNKPTQEEIAVQWLLHSPFDRSNGVRKKVIATLQIDQDQLAPLETLGYE
VVPVTSLRDLNTEVDAIWFDETVQSIEGEVPTDTIVFASDRQSTALKDAIVITNQSPAKIALQLRNGEESRQPIKI

Sequences:

>Translated_556_residues
MSKFQSLQAETILLHGGQEPDPSTGSRAVPIYQTTSYVFRDTEHAQNLFGLAEPGNIYSRIMNPTVDAFEQRITQLEDGI
GAVATSSGMAAITLAILNIAGSGDEIVADSNLYGGTFNLFANTLPRYGINVKFVDGTDPQAIDDAITDKTKAVFGEIITN
PSLYVFDIEKVSEVAHKHGVPLIIDNTFATPYVTKPLTWGADIVVHSATKWIGGHGTTIGGVVVDSGRFDWNNDRFQDFA
EPDESYNGLKYVDLGAAAYITKLRVQLLRDIGASLSPQNAFLLLQGLETLHLRIKQHTDNALQLAKYLQAHPAVEWVNYP
GLEEHPSHELAGKYLKQSFGSIITFGISGGREAGRTLIDNVSLWSHVANVGDAKSLIIHPASTTHQQLNEDDLALSGVTE
ELVRLSVGLESIDDIIADLDQAIEKATGKSHTNKPTQEEIAVQWLLHSPFDRSNGVRKKVIATLQIDQDQLAPLETLGYE
VVPVTSLRDLNTEVDAIWFDETVQSIEGEVPTDTIVFASDRQSTALKDAIVITNQSPAKIALQLRNGEESRQPIKI
>Mature_555_residues
SKFQSLQAETILLHGGQEPDPSTGSRAVPIYQTTSYVFRDTEHAQNLFGLAEPGNIYSRIMNPTVDAFEQRITQLEDGIG
AVATSSGMAAITLAILNIAGSGDEIVADSNLYGGTFNLFANTLPRYGINVKFVDGTDPQAIDDAITDKTKAVFGEIITNP
SLYVFDIEKVSEVAHKHGVPLIIDNTFATPYVTKPLTWGADIVVHSATKWIGGHGTTIGGVVVDSGRFDWNNDRFQDFAE
PDESYNGLKYVDLGAAAYITKLRVQLLRDIGASLSPQNAFLLLQGLETLHLRIKQHTDNALQLAKYLQAHPAVEWVNYPG
LEEHPSHELAGKYLKQSFGSIITFGISGGREAGRTLIDNVSLWSHVANVGDAKSLIIHPASTTHQQLNEDDLALSGVTEE
LVRLSVGLESIDDIIADLDQAIEKATGKSHTNKPTQEEIAVQWLLHSPFDRSNGVRKKVIATLQIDQDQLAPLETLGYEV
VPVTSLRDLNTEVDAIWFDETVQSIEGEVPTDTIVFASDRQSTALKDAIVITNQSPAKIALQLRNGEESRQPIKI

Specific function: Methionine biosynthesis; second step. [C]

COG id: COG2873

COG function: function code E; O-acetylhomoserine sulfhydrylase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the trans-sulfuration enzymes family [H]

Homologues:

Organism=Homo sapiens, GI21361334, Length=427, Percent_Identity=33.9578454332553, Blast_Score=219, Evalue=5e-57,
Organism=Homo sapiens, GI299473759, Length=427, Percent_Identity=32.0843091334895, Blast_Score=181, Evalue=2e-45,
Organism=Homo sapiens, GI25453487, Length=426, Percent_Identity=29.3427230046948, Blast_Score=160, Evalue=3e-39,
Organism=Escherichia coli, GI1790375, Length=417, Percent_Identity=33.3333333333333, Blast_Score=222, Evalue=5e-59,
Organism=Escherichia coli, GI1789383, Length=427, Percent_Identity=26.6978922716628, Blast_Score=124, Evalue=2e-29,
Organism=Caenorhabditis elegans, GI71980708, Length=433, Percent_Identity=34.4110854503464, Blast_Score=238, Evalue=7e-63,
Organism=Caenorhabditis elegans, GI17538055, Length=423, Percent_Identity=35.6973995271868, Blast_Score=217, Evalue=1e-56,
Organism=Caenorhabditis elegans, GI17560056, Length=420, Percent_Identity=36.1904761904762, Blast_Score=209, Evalue=4e-54,
Organism=Saccharomyces cerevisiae, GI6323333, Length=431, Percent_Identity=50.8120649651972, Blast_Score=396, Evalue=1e-111,
Organism=Saccharomyces cerevisiae, GI6319307, Length=434, Percent_Identity=34.5622119815668, Blast_Score=205, Evalue=2e-53,
Organism=Saccharomyces cerevisiae, GI6321170, Length=324, Percent_Identity=28.0864197530864, Blast_Score=128, Evalue=2e-30,
Organism=Saccharomyces cerevisiae, GI6321254, Length=447, Percent_Identity=26.3982102908277, Blast_Score=120, Evalue=5e-28,
Organism=Saccharomyces cerevisiae, GI6321904, Length=426, Percent_Identity=26.2910798122066, Blast_Score=110, Evalue=6e-25,
Organism=Drosophila melanogaster, GI19922554, Length=421, Percent_Identity=33.4916864608076, Blast_Score=213, Evalue=4e-55,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR000277
- InterPro:   IPR006237
- InterPro:   IPR015424
- InterPro:   IPR015421
- InterPro:   IPR015422 [H]

Pfam domain/function: PF01053 Cys_Met_Meta_PP [H]

EC number: =4.4.1.11 [H]

Molecular weight: Translated: 60673; Mature: 60542

Theoretical pI: Translated: 4.61; Mature: 4.61

Prosite motif: PS00868 CYS_MET_METAB_PP

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.0 %Cys     (Translated Protein)
0.5 %Met     (Translated Protein)
0.5 %Cys+Met (Translated Protein)
0.0 %Cys     (Mature Protein)
0.4 %Met     (Mature Protein)
0.4 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MSKFQSLQAETILLHGGQEPDPSTGSRAVPIYQTTSYVFRDTEHAQNLFGLAEPGNIYSR
CCHHHHCCCCEEEEECCCCCCCCCCCEEEEEEECCHHHCCCCHHHHHHCCCCCCCHHHHH
IMNPTVDAFEQRITQLEDGIGAVATSSGMAAITLAILNIAGSGDEIVADSNLYGGTFNLF
HHCCCHHHHHHHHHHHHCCCCCEECCCCHHHEEEEHHHCCCCCCCEEECCCCCCCHHHHH
ANTLPRYGINVKFVDGTDPQAIDDAITDKTKAVFGEIITNPSLYVFDIEKVSEVAHKHGV
HHCCCCCCCEEEEECCCCCHHHHHHHHHHHHHHHHHHHCCCCEEEEEHHHHHHHHHHCCC
PLIIDNTFATPYVTKPLTWGADIVVHSATKWIGGHGTTIGGVVVDSGRFDWNNDRFQDFA
CEEEECCCCCCCCCCCCCCCCCEEEECCHHHCCCCCCEECEEEEECCCCCCCCCHHHHHC
EPDESYNGLKYVDLGAAAYITKLRVQLLRDIGASLSPQNAFLLLQGLETLHLRIKQHTDN
CCCCCCCCEEEEECCHHHHHHHHHHHHHHHHCCCCCCCCCEEEECCHHHHEEHHHHCCCH
ALQLAKYLQAHPAVEWVNYPGLEEHPSHELAGKYLKQSFGSIITFGISGGREAGRTLIDN
HHHHHHHHHHCCCEEECCCCCCCCCCCHHHHHHHHHHHCCCEEEEECCCCHHHHHHHHHH
VSLWSHVANVGDAKSLIIHPASTTHQQLNEDDLALSGVTEELVRLSVGLESIDDIIADLD
HHHHHHHHCCCCCCEEEEECCCCHHHHCCCCCHHHHHHHHHHHHHHHCHHHHHHHHHHHH
QAIEKATGKSHTNKPTQEEIAVQWLLHSPFDRSNGVRKKVIATLQIDQDQLAPLETLGYE
HHHHHHCCCCCCCCCCHHHHHHHHHHCCCCCCCCCCHHHEEEEEEECHHHCCCHHHCCEE
VVPVTSLRDLNTEVDAIWFDETVQSIEGEVPTDTIVFASDRQSTALKDAIVITNQSPAKI
EEEECCHHHCCCCCEEEEHHHHHHHHCCCCCCCEEEEECCCCCHHHCCEEEEECCCCCEE
ALQLRNGEESRQPIKI
EEEEECCCCCCCCCCC
>Mature Secondary Structure 
SKFQSLQAETILLHGGQEPDPSTGSRAVPIYQTTSYVFRDTEHAQNLFGLAEPGNIYSR
CHHHHCCCCEEEEECCCCCCCCCCCEEEEEEECCHHHCCCCHHHHHHCCCCCCCHHHHH
IMNPTVDAFEQRITQLEDGIGAVATSSGMAAITLAILNIAGSGDEIVADSNLYGGTFNLF
HHCCCHHHHHHHHHHHHCCCCCEECCCCHHHEEEEHHHCCCCCCCEEECCCCCCCHHHHH
ANTLPRYGINVKFVDGTDPQAIDDAITDKTKAVFGEIITNPSLYVFDIEKVSEVAHKHGV
HHCCCCCCCEEEEECCCCCHHHHHHHHHHHHHHHHHHHCCCCEEEEEHHHHHHHHHHCCC
PLIIDNTFATPYVTKPLTWGADIVVHSATKWIGGHGTTIGGVVVDSGRFDWNNDRFQDFA
CEEEECCCCCCCCCCCCCCCCCEEEECCHHHCCCCCCEECEEEEECCCCCCCCCHHHHHC
EPDESYNGLKYVDLGAAAYITKLRVQLLRDIGASLSPQNAFLLLQGLETLHLRIKQHTDN
CCCCCCCCEEEEECCHHHHHHHHHHHHHHHHCCCCCCCCCEEEECCHHHHEEHHHHCCCH
ALQLAKYLQAHPAVEWVNYPGLEEHPSHELAGKYLKQSFGSIITFGISGGREAGRTLIDN
HHHHHHHHHHCCCEEECCCCCCCCCCCHHHHHHHHHHHCCCEEEEECCCCHHHHHHHHHH
VSLWSHVANVGDAKSLIIHPASTTHQQLNEDDLALSGVTEELVRLSVGLESIDDIIADLD
HHHHHHHHCCCCCCEEEEECCCCHHHHCCCCCHHHHHHHHHHHHHHHCHHHHHHHHHHHH
QAIEKATGKSHTNKPTQEEIAVQWLLHSPFDRSNGVRKKVIATLQIDQDQLAPLETLGYE
HHHHHHCCCCCCCCCCHHHHHHHHHHCCCCCCCCCCHHHEEEEEEECHHHCCCHHHCCEE
VVPVTSLRDLNTEVDAIWFDETVQSIEGEVPTDTIVFASDRQSTALKDAIVITNQSPAKI
EEEECCHHHCCCCCEEEEHHHHHHHHCCCCCCCEEEEECCCCCHHHCCEEEEECCCCCEE
ALQLRNGEESRQPIKI
EEEEECCCCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 8586629; 9190812; 3365412 [H]