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The map label for this gene is cwlJ

Identifier: 23100479

GI number: 23100479

Start: 3136579

End: 3137004

Strand: Direct

Name: cwlJ

Synonym: OB3024

Alternate gene names: 23100479

Gene position: 3136579-3137004 (Clockwise)

Preceding gene: 23100478

Following gene: 23100480

Centisome position: 86.39

GC content: 42.49

Gene sequence:

>426_bases
ATGGCCGTTGTGAAACATAACGAGGCAGAAGTAGAATTACTTGCCCGTCTCATGCGTGCTGAAGCTGAGGGTGATGGTGA
CTTAGGAATGTTGATGGTTGGAAACACCGGTATTAATAGGGTGATTGCAAGTTGTTTAGATTTCCCAAACATCCGCACTA
TAACCAATATGGTTTATCAAAGTCCCGGTGGATTTGAAGCAACACAGAAAGGCTATTTTTATCAAAGAGCACGTCAATCT
GAAATACGTCTTGCTCGAAGGGTGATACAAGGAGAAAGGCAACATCCTGCCTCAAATTCGTTATGGTTTTTTATGCCTGA
AGGCGCATGTCCGGAACAATGGTATGGTCAGTGGAATGTTGGTAGATATAAGGCTCATTGTTTTTATGCACCAACTGCTG
CAGATTGTCCAGAAGTATTTCGTTAA

Upstream 100 bases:

>100_bases
GGAAAGTCTTTTTTTATTGTCATTTTATTCGAAATACAGGCATAAAAATAAATATTGAGATCGACATTTTAGATGAGAAA
TATGGAAATGAGGTATCTCT

Downstream 100 bases:

>100_bases
GCGAGCTTATTTAGATTCGCTAAATCTATGAACAGATTAGGAGGGAACATCCATGAGTGAAAATGAAAACCAGTCCAACT
ATCAAAACTATCCTTATCAA

Product: cell wall hydrolase

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 141; Mature: 140

Protein sequence:

>141_residues
MAVVKHNEAEVELLARLMRAEAEGDGDLGMLMVGNTGINRVIASCLDFPNIRTITNMVYQSPGGFEATQKGYFYQRARQS
EIRLARRVIQGERQHPASNSLWFFMPEGACPEQWYGQWNVGRYKAHCFYAPTAADCPEVFR

Sequences:

>Translated_141_residues
MAVVKHNEAEVELLARLMRAEAEGDGDLGMLMVGNTGINRVIASCLDFPNIRTITNMVYQSPGGFEATQKGYFYQRARQS
EIRLARRVIQGERQHPASNSLWFFMPEGACPEQWYGQWNVGRYKAHCFYAPTAADCPEVFR
>Mature_140_residues
AVVKHNEAEVELLARLMRAEAEGDGDLGMLMVGNTGINRVIASCLDFPNIRTITNMVYQSPGGFEATQKGYFYQRARQSE
IRLARRVIQGERQHPASNSLWFFMPEGACPEQWYGQWNVGRYKAHCFYAPTAADCPEVFR

Specific function: Probable spore cortex-lytic enzyme involved in the depolymerization of cortex peptidoglycan during germination

COG id: COG3773

COG function: function code M; Cell wall hydrolyses involved in spore germination

Gene ontology:

Cell location: Secreted (Potential)

Metaboloic importance: NA

Operon status: Not Known

Operon components: None

Similarity: Belongs to the CwlJ family

Homologues:

None

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): CWLJ_OCEIH (P59104)

Other databases:

- EMBL:   BA000028
- RefSeq:   NP_693946.1
- GeneID:   1017903
- GenomeReviews:   BA000028_GR
- KEGG:   oih:OB3024
- NMPDR:   fig|221109.1.peg.3026
- HOGENOM:   HBG313941
- OMA:   YKSHCFF
- ProtClustDB:   CLSK873716
- BioCyc:   OIHE221109:OB3024-MONOMER
- InterPro:   IPR011105

Pfam domain/function: PF07486 Hydrolase_2

EC number: 3.5.1.28

Molecular weight: Translated: 16008; Mature: 15877

Theoretical pI: Translated: 7.38; Mature: 7.38

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

2.8 %Cys     (Translated Protein)
4.3 %Met     (Translated Protein)
7.1 %Cys+Met (Translated Protein)
2.9 %Cys     (Mature Protein)
3.6 %Met     (Mature Protein)
6.4 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MAVVKHNEAEVELLARLMRAEAEGDGDLGMLMVGNTGINRVIASCLDFPNIRTITNMVYQ
CCCCCCCHHHHHHHHHHHHHCCCCCCCEEEEEECCCCHHHHHHHHHCCCCHHHHHHHHHC
SPGGFEATQKGYFYQRARQSEIRLARRVIQGERQHPASNSLWFFMPEGACPEQWYGQWNV
CCCCCCCCCCCHHHHHHHHHHHHHHHHHHHCCCCCCCCCCEEEEECCCCCCHHHCCCCCC
GRYKAHCFYAPTAADCPEVFR
CEEEEEEEECCCCCCCHHHCC
>Mature Secondary Structure 
AVVKHNEAEVELLARLMRAEAEGDGDLGMLMVGNTGINRVIASCLDFPNIRTITNMVYQ
CCCCCCHHHHHHHHHHHHHCCCCCCCEEEEEECCCCHHHHHHHHHCCCCHHHHHHHHHC
SPGGFEATQKGYFYQRARQSEIRLARRVIQGERQHPASNSLWFFMPEGACPEQWYGQWNV
CCCCCCCCCCCHHHHHHHHHHHHHHHHHHHCCCCCCCCCCEEEEECCCCCCHHHCCCCCC
GRYKAHCFYAPTAADCPEVFR
CEEEEEEEECCCCCCCHHHCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 12235376