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The map label for this gene is pbpG [H]

Identifier: 23100472

GI number: 23100472

Start: 3128306

End: 3130333

Strand: Direct

Name: pbpG [H]

Synonym: OB3017

Alternate gene names: 23100472

Gene position: 3128306-3130333 (Clockwise)

Preceding gene: 23100444

Following gene: 23100476

Centisome position: 86.17

GC content: 36.74

Gene sequence:

>2028_bases
ATGATGAAGCAATCGAAAATTTGGAAAAGAATAAGAACCATTATATGGGTAATCGTTGGTTTAGTTATAACGGCTATACT
AGGTACATATATCATTTCTTATCTACTCGGACCACCAGAATTAGCTGGTAAACAACCAACAACCGTCTACGATCGATATG
ATGAAAAAATTGGAGAAGAACGCTTGTTAAACTCCATGGAATGGGTCCCGCTTGAAGATATGAACAAAAATATCATCCAA
GCCACCTTACTTATCGAAGATCAGCAGTTTTTTGAACATAATGGATTTAATTATCAACGAATCGCAAAAGCGATTTGGAC
AAATATAACGCATGGTTCCTTAAAAGAAGGAGCTAGTACAATAACACAACAATATGCAAGGAATCTTTATCTCTCTCCTG
AAAAAAAATGGTCTCGAAAAATACAGGAGGCTTTTTATGCTATACGTTTAGAAATGTTTTACTCAAAAGAAGAGATTTTA
GAAGGATATCTAAATACCATCTATTACGGACATGGCGCTTATGGAATTGAAGCTGCTAGTCGTTATTTTTTTGATACGAC
AGCAACAGATTTATCATTAGCGCAAGCAGCAATGCTAGCAGGAATTCCTAAAGGACCCACCTATTATTCTCCATTAAATA
ATGAAAAATTGGCTAATGAACGTCAACAACTTATATTATCTGAAATGCTTCAACATGGTGTGATTACACAAGAGGGGTAC
AATCGTGCAACCCAAGAAAAATTAATCTATGCTGAAACTGCTGCAGAGGAAGAAGTTGCACCATACTTCTCCGATGAAGC
GGTTTTAGAAGCTGCTGAACTATTAGACATTGCTACTGAAGAAATACAATCAGGTGGATATGAATTATATACTACATTAG
ATCTATCTCATCAATCAGAAATGGATGAGGCAGTATCCAAGAATATGAATGGTTCAGAGGATTTAGAAACAGCTGGGGTG
TCTATTAACCCTAAAACTGGAGAAATTACTGCCATGACTGGCGGAAAAAATTACCTAACCAGTAGTTATAATCGATCTAC
CAAATCAAAAAGAATGGCAGGTTCTTCTTTCAAACCAATCCTTTATTATGAAGCTCTCGAGAATGGTTATACACCAAGTA
CTCGATTGGTGAGCGAGGCGACGACATTTACTTTAGAAGATGGGACAACCTATCAGCCTGGAAATTTTAACGGCTATTAC
GCGAATGGCCCAATTACTCTAGCACAAGCGATTGCACTTTCTGATAATATCTATGCAGTAAAGACTAATTTGTATCTTGG
GCCGAAAAATCTAGTGGAAACAGCTAAAGATTTTGGTATTACAAGTGAACTTGATGCTGTACCTTCCTTAGCTTTAGGTA
CTTCATCTATTAGCGTCTTAGAATTAACGCAAGCTTATGGCAGGATAGCTAACGGTGGAAAAGAAATTGAACCCTATACC
ATACGAAAAATAATCGATAAAAAAGGAAATACAGTTTATGAGCATGATAATGATAAATTAGATCAAGTTTTAGACGAGAA
AAATACGTTTATACTTACTCAATTATTGACCGGTATGTTTGATGAAGAATTAAATAATTATACAAGTGTTACTGGTTCGA
CGATTAATCATCAACTTTCCAACATTTATGCTGGTAAATCAGGTACGACAGATTACGATAGCTGGATGGTAGGTTATAGT
CCGGAGGTTGTTACTGGTATATGGGTAGGTTATGATGATCACCGTAAAATAACGCAAGTTCAAGAAACTACTTTCTCTAA
ACAAATCTGGGCTAATTATATGGAAGCTATTCACGAACCATCAAGCAAACACACATTTGCGATGCCTTCTGGGGTAATCG
GCTTACCAATTGATCCTGTGAGCGGAAAGAGAGCTACATCCTCCTGTGGCCAAAGTCGTATTATGTTTTTTGAGAAAGGT
ACAGAACCACTCGGCTACTGTACAGATAGAAATCATCCCGAAGATCTAGAGGAAGAAGAAAATGATCAGAAGGGCTTCAT
GAAAAAAATCTTTGATGAATTCTTCTAG

Upstream 100 bases:

>100_bases
AATACTATTCGTTAGTGCAAGAATTTTCGAACAAACGTTTATATTCATGATTCCTTTCCAATAATGGTTATATAACGCTT
GAAAAGTAATGGAGACGAAT

Downstream 100 bases:

>100_bases
GTTACATTAGAATTCACACAAAAAAACAGCCAGTATCGAGGGTATCGATAGCTGGCTGTTTTTTTGTCCTAGTAAACATG
AGTGAGCCCATGTATTCAAT

Product: penicillin-binding protein

Products: NA

Alternate protein names: PBP-2D; PBP2d [H]

Number of amino acids: Translated: 675; Mature: 675

Protein sequence:

>675_residues
MMKQSKIWKRIRTIIWVIVGLVITAILGTYIISYLLGPPELAGKQPTTVYDRYDEKIGEERLLNSMEWVPLEDMNKNIIQ
ATLLIEDQQFFEHNGFNYQRIAKAIWTNITHGSLKEGASTITQQYARNLYLSPEKKWSRKIQEAFYAIRLEMFYSKEEIL
EGYLNTIYYGHGAYGIEAASRYFFDTTATDLSLAQAAMLAGIPKGPTYYSPLNNEKLANERQQLILSEMLQHGVITQEGY
NRATQEKLIYAETAAEEEVAPYFSDEAVLEAAELLDIATEEIQSGGYELYTTLDLSHQSEMDEAVSKNMNGSEDLETAGV
SINPKTGEITAMTGGKNYLTSSYNRSTKSKRMAGSSFKPILYYEALENGYTPSTRLVSEATTFTLEDGTTYQPGNFNGYY
ANGPITLAQAIALSDNIYAVKTNLYLGPKNLVETAKDFGITSELDAVPSLALGTSSISVLELTQAYGRIANGGKEIEPYT
IRKIIDKKGNTVYEHDNDKLDQVLDEKNTFILTQLLTGMFDEELNNYTSVTGSTINHQLSNIYAGKSGTTDYDSWMVGYS
PEVVTGIWVGYDDHRKITQVQETTFSKQIWANYMEAIHEPSSKHTFAMPSGVIGLPIDPVSGKRATSSCGQSRIMFFEKG
TEPLGYCTDRNHPEDLEEEENDQKGFMKKIFDEFF

Sequences:

>Translated_675_residues
MMKQSKIWKRIRTIIWVIVGLVITAILGTYIISYLLGPPELAGKQPTTVYDRYDEKIGEERLLNSMEWVPLEDMNKNIIQ
ATLLIEDQQFFEHNGFNYQRIAKAIWTNITHGSLKEGASTITQQYARNLYLSPEKKWSRKIQEAFYAIRLEMFYSKEEIL
EGYLNTIYYGHGAYGIEAASRYFFDTTATDLSLAQAAMLAGIPKGPTYYSPLNNEKLANERQQLILSEMLQHGVITQEGY
NRATQEKLIYAETAAEEEVAPYFSDEAVLEAAELLDIATEEIQSGGYELYTTLDLSHQSEMDEAVSKNMNGSEDLETAGV
SINPKTGEITAMTGGKNYLTSSYNRSTKSKRMAGSSFKPILYYEALENGYTPSTRLVSEATTFTLEDGTTYQPGNFNGYY
ANGPITLAQAIALSDNIYAVKTNLYLGPKNLVETAKDFGITSELDAVPSLALGTSSISVLELTQAYGRIANGGKEIEPYT
IRKIIDKKGNTVYEHDNDKLDQVLDEKNTFILTQLLTGMFDEELNNYTSVTGSTINHQLSNIYAGKSGTTDYDSWMVGYS
PEVVTGIWVGYDDHRKITQVQETTFSKQIWANYMEAIHEPSSKHTFAMPSGVIGLPIDPVSGKRATSSCGQSRIMFFEKG
TEPLGYCTDRNHPEDLEEEENDQKGFMKKIFDEFF
>Mature_675_residues
MMKQSKIWKRIRTIIWVIVGLVITAILGTYIISYLLGPPELAGKQPTTVYDRYDEKIGEERLLNSMEWVPLEDMNKNIIQ
ATLLIEDQQFFEHNGFNYQRIAKAIWTNITHGSLKEGASTITQQYARNLYLSPEKKWSRKIQEAFYAIRLEMFYSKEEIL
EGYLNTIYYGHGAYGIEAASRYFFDTTATDLSLAQAAMLAGIPKGPTYYSPLNNEKLANERQQLILSEMLQHGVITQEGY
NRATQEKLIYAETAAEEEVAPYFSDEAVLEAAELLDIATEEIQSGGYELYTTLDLSHQSEMDEAVSKNMNGSEDLETAGV
SINPKTGEITAMTGGKNYLTSSYNRSTKSKRMAGSSFKPILYYEALENGYTPSTRLVSEATTFTLEDGTTYQPGNFNGYY
ANGPITLAQAIALSDNIYAVKTNLYLGPKNLVETAKDFGITSELDAVPSLALGTSSISVLELTQAYGRIANGGKEIEPYT
IRKIIDKKGNTVYEHDNDKLDQVLDEKNTFILTQLLTGMFDEELNNYTSVTGSTINHQLSNIYAGKSGTTDYDSWMVGYS
PEVVTGIWVGYDDHRKITQVQETTFSKQIWANYMEAIHEPSSKHTFAMPSGVIGLPIDPVSGKRATSSCGQSRIMFFEKG
TEPLGYCTDRNHPEDLEEEENDQKGFMKKIFDEFF

Specific function: Involved in the polymerization and cross-linking of spore peptidoglycan. May be required for synthesis of the spore germ cell wall, the first layer of peptidoglycan synthesized on the surface of the inner forespore membrane [H]

COG id: COG0744

COG function: function code M; Membrane carboxypeptidase (penicillin-binding protein)

Gene ontology:

Cell location: Cell membrane; Single-pass membrane protein [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: In the C-terminal section; belongs to the transpeptidase family [H]

Homologues:

Organism=Escherichia coli, GI1786343, Length=554, Percent_Identity=29.4223826714801, Blast_Score=216, Evalue=4e-57,
Organism=Escherichia coli, GI87082258, Length=269, Percent_Identity=37.546468401487, Blast_Score=166, Evalue=5e-42,
Organism=Escherichia coli, GI1788867, Length=517, Percent_Identity=27.0793036750484, Blast_Score=154, Evalue=2e-38,
Organism=Escherichia coli, GI1789601, Length=176, Percent_Identity=35.2272727272727, Blast_Score=108, Evalue=1e-24,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR012338
- InterPro:   IPR001264
- InterPro:   IPR011816
- InterPro:   IPR001460 [H]

Pfam domain/function: PF00912 Transgly; PF00905 Transpeptidase [H]

EC number: 2.4.1.129; 3.4.-.- [C]

Molecular weight: Translated: 75779; Mature: 75779

Theoretical pI: Translated: 4.56; Mature: 4.56

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.3 %Cys     (Translated Protein)
2.5 %Met     (Translated Protein)
2.8 %Cys+Met (Translated Protein)
0.3 %Cys     (Mature Protein)
2.5 %Met     (Mature Protein)
2.8 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MMKQSKIWKRIRTIIWVIVGLVITAILGTYIISYLLGPPELAGKQPTTVYDRYDEKIGEE
CCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCHHHCCCCCCHHHHHHHHHHCHH
RLLNSMEWVPLEDMNKNIIQATLLIEDQQFFEHNGFNYQRIAKAIWTNITHGSLKEGAST
HHHHCCCCCCHHHCCCCCEEEEEEECHHHHHHCCCCCHHHHHHHHHHCCCCCHHHHHHHH
ITQQYARNLYLSPEKKWSRKIQEAFYAIRLEMFYSKEEILEGYLNTIYYGHGAYGIEAAS
HHHHHHHCCCCCCHHHHHHHHHHHHHHHHHHHHHCHHHHHHHHHHHEEECCCCCCHHHHH
RYFFDTTATDLSLAQAAMLAGIPKGPTYYSPLNNEKLANERQQLILSEMLQHGVITQEGY
HHEECCCHHHHHHHHHHHHHCCCCCCCCCCCCCCHHHHHHHHHHHHHHHHHCCCCCCCCC
NRATQEKLIYAETAAEEEVAPYFSDEAVLEAAELLDIATEEIQSGGYELYTTLDLSHQSE
CHHHHHHEEEEHHCCCHHCCCCCCCHHHHHHHHHHHHHHHHHHCCCEEEEEEEECCCHHH
MDEAVSKNMNGSEDLETAGVSINPKTGEITAMTGGKNYLTSSYNRSTKSKRMAGSSFKPI
HHHHHHCCCCCCCCHHHCCCEECCCCCEEEEEECCCHHHCCCCCCCHHHHHHCCCCCCCE
LYYEALENGYTPSTRLVSEATTFTLEDGTTYQPGNFNGYYANGPITLAQAIALSDNIYAV
EEHHHHHCCCCCCHHHHHHCEEEEECCCCEECCCCCCCEEECCCCHHHHHHHHCCCEEEE
KTNLYLGPKNLVETAKDFGITSELDAVPSLALGTSSISVLELTQAYGRIANGGKEIEPYT
EEEEEECCHHHHHHHHHCCCCHHHHHCCCHHCCCCCHHHHHHHHHHHHHHCCCCCCCCHH
IRKIIDKKGNTVYEHDNDKLDQVLDEKNTFILTQLLTGMFDEELNNYTSVTGSTINHQLS
HHHHHHCCCCEEEECCCHHHHHHHCCCCCCHHHHHHHHHHHHHHHHHHCCCCCHHHHHHH
NIYAGKSGTTDYDSWMVGYSPEVVTGIWVGYDDHRKITQVQETTFSKQIWANYMEAIHEP
HHCCCCCCCCCCCCEEECCCCCEEEEEEECCCCCHHHHHHHHHHHHHHHHHHHHHHHCCC
SSKHTFAMPSGVIGLPIDPVSGKRATSSCGQSRIMFFEKGTEPLGYCTDRNHPEDLEEEE
CCCCEEECCCCEEECCCCCCCCCCHHHHCCCCEEEEEECCCCCCCCCCCCCCCCCCHHHC
NDQKGFMKKIFDEFF
CHHHHHHHHHHHHCC
>Mature Secondary Structure
MMKQSKIWKRIRTIIWVIVGLVITAILGTYIISYLLGPPELAGKQPTTVYDRYDEKIGEE
CCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCHHHCCCCCCHHHHHHHHHHCHH
RLLNSMEWVPLEDMNKNIIQATLLIEDQQFFEHNGFNYQRIAKAIWTNITHGSLKEGAST
HHHHCCCCCCHHHCCCCCEEEEEEECHHHHHHCCCCCHHHHHHHHHHCCCCCHHHHHHHH
ITQQYARNLYLSPEKKWSRKIQEAFYAIRLEMFYSKEEILEGYLNTIYYGHGAYGIEAAS
HHHHHHHCCCCCCHHHHHHHHHHHHHHHHHHHHHCHHHHHHHHHHHEEECCCCCCHHHHH
RYFFDTTATDLSLAQAAMLAGIPKGPTYYSPLNNEKLANERQQLILSEMLQHGVITQEGY
HHEECCCHHHHHHHHHHHHHCCCCCCCCCCCCCCHHHHHHHHHHHHHHHHHCCCCCCCCC
NRATQEKLIYAETAAEEEVAPYFSDEAVLEAAELLDIATEEIQSGGYELYTTLDLSHQSE
CHHHHHHEEEEHHCCCHHCCCCCCCHHHHHHHHHHHHHHHHHHCCCEEEEEEEECCCHHH
MDEAVSKNMNGSEDLETAGVSINPKTGEITAMTGGKNYLTSSYNRSTKSKRMAGSSFKPI
HHHHHHCCCCCCCCHHHCCCEECCCCCEEEEEECCCHHHCCCCCCCHHHHHHCCCCCCCE
LYYEALENGYTPSTRLVSEATTFTLEDGTTYQPGNFNGYYANGPITLAQAIALSDNIYAV
EEHHHHHCCCCCCHHHHHHCEEEEECCCCEECCCCCCCEEECCCCHHHHHHHHCCCEEEE
KTNLYLGPKNLVETAKDFGITSELDAVPSLALGTSSISVLELTQAYGRIANGGKEIEPYT
EEEEEECCHHHHHHHHHCCCCHHHHHCCCHHCCCCCHHHHHHHHHHHHHHCCCCCCCCHH
IRKIIDKKGNTVYEHDNDKLDQVLDEKNTFILTQLLTGMFDEELNNYTSVTGSTINHQLS
HHHHHHCCCCEEEECCCHHHHHHHCCCCCCHHHHHHHHHHHHHHHHHHCCCCCHHHHHHH
NIYAGKSGTTDYDSWMVGYSPEVVTGIWVGYDDHRKITQVQETTFSKQIWANYMEAIHEP
HHCCCCCCCCCCCCEEECCCCCEEEEEEECCCCCHHHHHHHHHHHHHHHHHHHHHHHCCC
SSKHTFAMPSGVIGLPIDPVSGKRATSSCGQSRIMFFEKGTEPLGYCTDRNHPEDLEEEE
CCCCEEECCCCEEECCCCCCCCCCHHHHCCCCEEEEEECCCCCCCCCCCCCCCCCCHHHC
NDQKGFMKKIFDEFF
CHHHHHHHHHHHHCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: Transferases; Acyltransferases; Aminoacyltransferases [C]

Inhibitor: NA

Structure determination priority: 6.0

TargetDB status: NA

Availability: NA

References: 9353933; 9384377 [H]