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The map label for this gene is mccB [H]

Identifier: 23100404

GI number: 23100404

Start: 3055701

End: 3056906

Strand: Reverse

Name: mccB [H]

Synonym: OB2949

Alternate gene names: 23100404

Gene position: 3056906-3055701 (Counterclockwise)

Preceding gene: 23100405

Following gene: 23100403

Centisome position: 84.2

GC content: 39.55

Gene sequence:

>1206_bases
ATGACAGATAAGAAGAAAGTAAACGTAGGTACAGAAGCAGTTTGGGCGGGAGAAAAGGAATATCTTGTACATGGAGCAAC
GCAAGTACCTGTTGTCCTTAGTGTTGCCTACAATTATGACGATATGGATGAATGGTATGATGTAGCAATTGGAAAAAAGA
AGGGGCATATTTATGGACGAAACACAAACCCAACAGTTCAAGCTTTTGAAGATAAAGTGAAGCAGTTAGAAGGAGCAGAG
GCTGCTACTAGTTTTTCTACAGGAATGGCAGCAATAAGTAATACACTCTCTACGTTTTTATTACCAGGAGATCGCATTGT
TTCGATTAAAGACACGTATGGTGGAACGAATAAAATATTTACTGAATTTCTTCCTCGTCAGCAAGTAGAAGTAACGTTAT
GTGAGACTGGAGATCATGAACAAATTGAAAAAGAAGTGAATAAAGGCTGTAAGATTCTATATTTAGAATCACCAACAAAT
CCTACTGTGAAAATTACCGATCTGAAGAAAATGGCGGAAGTAGGAAAACGAGCAGGAGCCATTGTGATCGTCGATAACAC
GTTCGGTACTCCAATAAATCAAAATCCATTAGCACTTGGAGCCGATTTGGTTATTCATAGTGCTACAAAATTTCTAGGTG
GTCATGCAGATGCGTTAGGAGGAGTAGTTTGTGGCCCTGAAGATTATGTAGAGCAAATTTACCATTTTCGTGAAATAAAT
GGAGCAACAATGGATCCAATGGCTGCTTATTTAACATTACGTGGCATGAAGACATTGCATCTACGGGTAAGGGAACAATC
ATTTAATGCAATGCAATTAGCAAAGTTCCTACAGCAGCAAGATATTGTAGAAGAAGTGTTTTACCCAGGATTAACGACAC
ATCCGAATCATGATATTGCAAAAGAACAAATGAAAGACTTTGGCGGTATGTTAAGTTTTTCAGTAAAAGGCGGCGTGGAC
ACGGTAAGAGAATTGTTACCGAAGCTGCAGTATGCACATCGAGCAGCAAATCTAGGTGCAGTGGAAACAACAGTCGGCCC
TGCACGTACAACAAGTCATGTCGAATGTACACCAGAGGAACGGGCAGCAATGGGAATTCCAGAAGGGCTTATTCGCGTGT
CCTGTGGTATTGAATATATTGATGATATATTGGAAGACTTTGCTCAAGCATTTGCTCATATAGAAAATAGCTTAGAAATT
TCTTAG

Upstream 100 bases:

>100_bases
GATGGGTTTGCTAACCTAGATTTTGTCGGTTAGGAATAAAAGTATATGGTGGCGTGGATGCAAGTATCGTAACAAACAAT
TTAAAAAAGGAAGTGATTAT

Downstream 100 bases:

>100_bases
CAGAACGAGGTTGGGGCAAAACACCAATGGTTTAGTCAAATGATGAGTGATCGATAGGATAAGCATAAGATGCTCTTAAA
GGTGCCGTTGGATAAGCGCA

Product: cystathionine gamma-synthase

Products: NA

Alternate protein names: Gamma-cystathionase; Homocysteine gamma-lyase [H]

Number of amino acids: Translated: 401; Mature: 400

Protein sequence:

>401_residues
MTDKKKVNVGTEAVWAGEKEYLVHGATQVPVVLSVAYNYDDMDEWYDVAIGKKKGHIYGRNTNPTVQAFEDKVKQLEGAE
AATSFSTGMAAISNTLSTFLLPGDRIVSIKDTYGGTNKIFTEFLPRQQVEVTLCETGDHEQIEKEVNKGCKILYLESPTN
PTVKITDLKKMAEVGKRAGAIVIVDNTFGTPINQNPLALGADLVIHSATKFLGGHADALGGVVCGPEDYVEQIYHFREIN
GATMDPMAAYLTLRGMKTLHLRVREQSFNAMQLAKFLQQQDIVEEVFYPGLTTHPNHDIAKEQMKDFGGMLSFSVKGGVD
TVRELLPKLQYAHRAANLGAVETTVGPARTTSHVECTPEERAAMGIPEGLIRVSCGIEYIDDILEDFAQAFAHIENSLEI
S

Sequences:

>Translated_401_residues
MTDKKKVNVGTEAVWAGEKEYLVHGATQVPVVLSVAYNYDDMDEWYDVAIGKKKGHIYGRNTNPTVQAFEDKVKQLEGAE
AATSFSTGMAAISNTLSTFLLPGDRIVSIKDTYGGTNKIFTEFLPRQQVEVTLCETGDHEQIEKEVNKGCKILYLESPTN
PTVKITDLKKMAEVGKRAGAIVIVDNTFGTPINQNPLALGADLVIHSATKFLGGHADALGGVVCGPEDYVEQIYHFREIN
GATMDPMAAYLTLRGMKTLHLRVREQSFNAMQLAKFLQQQDIVEEVFYPGLTTHPNHDIAKEQMKDFGGMLSFSVKGGVD
TVRELLPKLQYAHRAANLGAVETTVGPARTTSHVECTPEERAAMGIPEGLIRVSCGIEYIDDILEDFAQAFAHIENSLEI
S
>Mature_400_residues
TDKKKVNVGTEAVWAGEKEYLVHGATQVPVVLSVAYNYDDMDEWYDVAIGKKKGHIYGRNTNPTVQAFEDKVKQLEGAEA
ATSFSTGMAAISNTLSTFLLPGDRIVSIKDTYGGTNKIFTEFLPRQQVEVTLCETGDHEQIEKEVNKGCKILYLESPTNP
TVKITDLKKMAEVGKRAGAIVIVDNTFGTPINQNPLALGADLVIHSATKFLGGHADALGGVVCGPEDYVEQIYHFREING
ATMDPMAAYLTLRGMKTLHLRVREQSFNAMQLAKFLQQQDIVEEVFYPGLTTHPNHDIAKEQMKDFGGMLSFSVKGGVDT
VRELLPKLQYAHRAANLGAVETTVGPARTTSHVECTPEERAAMGIPEGLIRVSCGIEYIDDILEDFAQAFAHIENSLEIS

Specific function: Catalyzes the conversion of cystathionine to cysteine, and homocysteine to sulfide [H]

COG id: COG0626

COG function: function code E; Cystathionine beta-lyases/cystathionine gamma-synthases

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the trans-sulfuration enzymes family [H]

Homologues:

Organism=Homo sapiens, GI21361334, Length=372, Percent_Identity=33.0645161290323, Blast_Score=216, Evalue=2e-56,
Organism=Homo sapiens, GI299473759, Length=372, Percent_Identity=29.5698924731183, Blast_Score=175, Evalue=8e-44,
Organism=Homo sapiens, GI25453487, Length=371, Percent_Identity=30.9973045822102, Blast_Score=162, Evalue=4e-40,
Organism=Escherichia coli, GI1790375, Length=392, Percent_Identity=36.734693877551, Blast_Score=258, Evalue=5e-70,
Organism=Escherichia coli, GI1789383, Length=407, Percent_Identity=26.044226044226, Blast_Score=139, Evalue=4e-34,
Organism=Caenorhabditis elegans, GI17538055, Length=374, Percent_Identity=35.8288770053476, Blast_Score=237, Evalue=8e-63,
Organism=Caenorhabditis elegans, GI17560056, Length=375, Percent_Identity=36, Blast_Score=231, Evalue=6e-61,
Organism=Caenorhabditis elegans, GI71980708, Length=368, Percent_Identity=33.695652173913, Blast_Score=211, Evalue=4e-55,
Organism=Saccharomyces cerevisiae, GI6319307, Length=406, Percent_Identity=36.9458128078818, Blast_Score=240, Evalue=2e-64,
Organism=Saccharomyces cerevisiae, GI6323333, Length=415, Percent_Identity=33.4939759036145, Blast_Score=189, Evalue=1e-48,
Organism=Saccharomyces cerevisiae, GI6321254, Length=395, Percent_Identity=29.873417721519, Blast_Score=156, Evalue=4e-39,
Organism=Saccharomyces cerevisiae, GI6321170, Length=295, Percent_Identity=30.1694915254237, Blast_Score=137, Evalue=3e-33,
Organism=Saccharomyces cerevisiae, GI6321904, Length=381, Percent_Identity=28.0839895013123, Blast_Score=134, Evalue=3e-32,
Organism=Drosophila melanogaster, GI19922554, Length=365, Percent_Identity=33.6986301369863, Blast_Score=223, Evalue=2e-58,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR000277
- InterPro:   IPR015424
- InterPro:   IPR015421
- InterPro:   IPR015422 [H]

Pfam domain/function: PF01053 Cys_Met_Meta_PP [H]

EC number: =4.4.1.1; =4.4.1.2 [H]

Molecular weight: Translated: 44016; Mature: 43884

Theoretical pI: Translated: 5.08; Mature: 5.08

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.2 %Cys     (Translated Protein)
2.7 %Met     (Translated Protein)
4.0 %Cys+Met (Translated Protein)
1.2 %Cys     (Mature Protein)
2.5 %Met     (Mature Protein)
3.8 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MTDKKKVNVGTEAVWAGEKEYLVHGATQVPVVLSVAYNYDDMDEWYDVAIGKKKGHIYGR
CCCCCEECCCCCEEECCCHHEEEECCCCCCEEEEEEECCCCHHHHHHHHCCCCCCEEECC
NTNPTVQAFEDKVKQLEGAEAATSFSTGMAAISNTLSTFLLPGDRIVSIKDTYGGTNKIF
CCCCHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHHCCCCCEEEEEECCCCCCHHHH
TEFLPRQQVEVTLCETGDHEQIEKEVNKGCKILYLESPTNPTVKITDLKKMAEVGKRAGA
HHHCCCCCEEEEEECCCCHHHHHHHHCCCCEEEEEECCCCCCEEEHHHHHHHHHHHHCCE
IVIVDNTFGTPINQNPLALGADLVIHSATKFLGGHADALGGVVCGPEDYVEQIYHFREIN
EEEEECCCCCCCCCCCCCCCHHHHHHHHHHHHCCCHHHHCCEEECCHHHHHHHHHHHHCC
GATMDPMAAYLTLRGMKTLHLRVREQSFNAMQLAKFLQQQDIVEEVFYPGLTTHPNHDIA
CCCCCHHHHHHHHCCCEEEEEEEECCCCCHHHHHHHHHHHHHHHHHHCCCCCCCCCCHHH
KEQMKDFGGMLSFSVKGGVDTVRELLPKLQYAHRAANLGAVETTVGPARTTSHVECTPEE
HHHHHHHCCEEEEEECCCHHHHHHHHHHHHHHHHHCCCCCEECCCCCCCCCCCEECCCHH
RAAMGIPEGLIRVSCGIEYIDDILEDFAQAFAHIENSLEIS
HHHCCCCHHHHEECCCHHHHHHHHHHHHHHHHHHHCCCCCC
>Mature Secondary Structure 
TDKKKVNVGTEAVWAGEKEYLVHGATQVPVVLSVAYNYDDMDEWYDVAIGKKKGHIYGR
CCCCEECCCCCEEECCCHHEEEECCCCCCEEEEEEECCCCHHHHHHHHCCCCCCEEECC
NTNPTVQAFEDKVKQLEGAEAATSFSTGMAAISNTLSTFLLPGDRIVSIKDTYGGTNKIF
CCCCHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHHCCCCCEEEEEECCCCCCHHHH
TEFLPRQQVEVTLCETGDHEQIEKEVNKGCKILYLESPTNPTVKITDLKKMAEVGKRAGA
HHHCCCCCEEEEEECCCCHHHHHHHHCCCCEEEEEECCCCCCEEEHHHHHHHHHHHHCCE
IVIVDNTFGTPINQNPLALGADLVIHSATKFLGGHADALGGVVCGPEDYVEQIYHFREIN
EEEEECCCCCCCCCCCCCCCHHHHHHHHHHHHCCCHHHHCCEEECCHHHHHHHHHHHHCC
GATMDPMAAYLTLRGMKTLHLRVREQSFNAMQLAKFLQQQDIVEEVFYPGLTTHPNHDIA
CCCCCHHHHHHHHCCCEEEEEEEECCCCCHHHHHHHHHHHHHHHHHHCCCCCCCCCCHHH
KEQMKDFGGMLSFSVKGGVDTVRELLPKLQYAHRAANLGAVETTVGPARTTSHVECTPEE
HHHHHHHCCEEEEEECCCHHHHHHHHHHHHHHHHHCCCCCEECCCCCCCCCCCEECCCHH
RAAMGIPEGLIRVSCGIEYIDDILEDFAQAFAHIENSLEIS
HHHCCCCHHHHEECCCHHHHHHHHHHHHHHHHHHHCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 9308178; 9384377 [H]