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The map label for this gene is cphA [H]

Identifier: 23100370

GI number: 23100370

Start: 3008744

End: 3010189

Strand: Reverse

Name: cphA [H]

Synonym: OB2915

Alternate gene names: 23100370

Gene position: 3010189-3008744 (Counterclockwise)

Preceding gene: 23100372

Following gene: 23100369

Centisome position: 82.91

GC content: 33.4

Gene sequence:

>1446_bases
ATGAAATCTCAAGAAGTGGTACTTCTTCCACACTTAAATAAAAGAGTAGTAAAAGATATAACAGGATTTAAGTTATGTTC
ATATTTAGTTGCATTAGAAGGTTGGAGAAGAGGATTAAACCTAACTTGGTATAATGATGAAACAAGTAAATGTAAGTTAG
ATCGCTTGAATAGTAGTACACATGGAAAGTTTTATTCATTAAGTGATGGTAGTAAAACGCATTATTTTTTCCGTTCTAGA
GGCGATAAAGTAACTAATAAAGCAGTTAGAATTTGTCAGGATAAGGAAGAAACGAAATCATATCTGCAGAAAAGCAATGT
ACCAATTCCCCTAGGAAAAGTATTAGAGGAAGATAATGATATCATAAACTATGCCAATGAAATAGATTATCCAGTAATTA
TAAAACCTCTCAAAGGAAGTATGGGTAAAGGTGTTTATACAAATATTAATTCAGAAGAAGAATTAAAGGGTATATTGAAA
GAATTAAGGTCTAAATTTTCCCACAAGGAATATTTGGTTGAAAAACATTATCCAGGTGAAGAGTACCGTATTTATGTAGT
CGGGGATCAAGTGATTGGTGCTACGAACAGAAAGCCGGCCAATATTATTGGAGATGGAAAGCACACCGTGCAGAAACTAA
TTGATCTAAAGAATGAAGAGAGAAAGAAGAACCCGTATTTATCTCCTAAACCAATCGAGGCAGATTATGAGATAACATTT
ATGCTTGAAAGAGTCGGGTATGATTTAAATAGTGTGCCTGAAAAAGGGGAACAAGTAATTTTAAGAGAGAAAAGTAATCT
TTCTTCTGGTGGTGATCCCATTGAAGCTACTGATGAATTATCTCCATCTGTTAGGCAAATAGCGGTAGATGCTTTAAAGG
CGTTGCCTTCTATTCCTCATGGTGGTGTTGATATAATTGTAGATCCAAGCGATCCAAGTAATGGTGTTGTACTTGAAATC
AACGCAACTGCTGAAATTGGATTCCATCCATTTCCTTTAATTGGCGAATCAAAGGATGTACCAGCAGCAATAATTGATTA
TTATTTCCCGGAAACAAAAGGGAAATACAGATCACCATTTTATTTTGATTATCTTAGCTTATTAGAACCTTTAAAGAGTT
GGGCGGTTGAAGAATTAAAGGTTGTAGAAACACCCAAAAAGGACGTTTATGCTAGGAAGTATATAGTAACAGGTCAACTT
AACAAAGTTGGGTATATGACTTATATTAGAAGACAAGCTTTAAGAAGAAATCTTTATGGTTATGCTAAGAAAATAACGAA
GAATGAAGTAGAGATTTATCTTATTAGTGAAAACCAGAAATCAGTTGAAGATTTTAAGAAATTTGTTCAAAAAGGATCAA
GAAAATCGATCGTAAAACACATTGAAGATAAAGAAGTAGAGTTTTCTGGGAAACCTACAAAAGTTGGTTTCCGAATAATA
AGCTAA

Upstream 100 bases:

>100_bases
ATTCATTCCATTTTGTAGTTATAGCTTTTTATTTATAATGTATTTAAATATAATATATAGTATAGAATGTTTCAATTATA
AGAATTTGAGGAGAATGATA

Downstream 100 bases:

>100_bases
GAATAGGAAAGTGGGATTTATTCATGCAAGAAATTGGTTTTATGACAAACTTTAATCAGCCGACAGAATTAGCAACTATC
ACAGCACTGATTGCCAAATC

Product: hypothetical protein

Products: NA

Alternate protein names: Cyanophycin synthase [H]

Number of amino acids: Translated: 481; Mature: 481

Protein sequence:

>481_residues
MKSQEVVLLPHLNKRVVKDITGFKLCSYLVALEGWRRGLNLTWYNDETSKCKLDRLNSSTHGKFYSLSDGSKTHYFFRSR
GDKVTNKAVRICQDKEETKSYLQKSNVPIPLGKVLEEDNDIINYANEIDYPVIIKPLKGSMGKGVYTNINSEEELKGILK
ELRSKFSHKEYLVEKHYPGEEYRIYVVGDQVIGATNRKPANIIGDGKHTVQKLIDLKNEERKKNPYLSPKPIEADYEITF
MLERVGYDLNSVPEKGEQVILREKSNLSSGGDPIEATDELSPSVRQIAVDALKALPSIPHGGVDIIVDPSDPSNGVVLEI
NATAEIGFHPFPLIGESKDVPAAIIDYYFPETKGKYRSPFYFDYLSLLEPLKSWAVEELKVVETPKKDVYARKYIVTGQL
NKVGYMTYIRRQALRRNLYGYAKKITKNEVEIYLISENQKSVEDFKKFVQKGSRKSIVKHIEDKEVEFSGKPTKVGFRII
S

Sequences:

>Translated_481_residues
MKSQEVVLLPHLNKRVVKDITGFKLCSYLVALEGWRRGLNLTWYNDETSKCKLDRLNSSTHGKFYSLSDGSKTHYFFRSR
GDKVTNKAVRICQDKEETKSYLQKSNVPIPLGKVLEEDNDIINYANEIDYPVIIKPLKGSMGKGVYTNINSEEELKGILK
ELRSKFSHKEYLVEKHYPGEEYRIYVVGDQVIGATNRKPANIIGDGKHTVQKLIDLKNEERKKNPYLSPKPIEADYEITF
MLERVGYDLNSVPEKGEQVILREKSNLSSGGDPIEATDELSPSVRQIAVDALKALPSIPHGGVDIIVDPSDPSNGVVLEI
NATAEIGFHPFPLIGESKDVPAAIIDYYFPETKGKYRSPFYFDYLSLLEPLKSWAVEELKVVETPKKDVYARKYIVTGQL
NKVGYMTYIRRQALRRNLYGYAKKITKNEVEIYLISENQKSVEDFKKFVQKGSRKSIVKHIEDKEVEFSGKPTKVGFRII
S
>Mature_481_residues
MKSQEVVLLPHLNKRVVKDITGFKLCSYLVALEGWRRGLNLTWYNDETSKCKLDRLNSSTHGKFYSLSDGSKTHYFFRSR
GDKVTNKAVRICQDKEETKSYLQKSNVPIPLGKVLEEDNDIINYANEIDYPVIIKPLKGSMGKGVYTNINSEEELKGILK
ELRSKFSHKEYLVEKHYPGEEYRIYVVGDQVIGATNRKPANIIGDGKHTVQKLIDLKNEERKKNPYLSPKPIEADYEITF
MLERVGYDLNSVPEKGEQVILREKSNLSSGGDPIEATDELSPSVRQIAVDALKALPSIPHGGVDIIVDPSDPSNGVVLEI
NATAEIGFHPFPLIGESKDVPAAIIDYYFPETKGKYRSPFYFDYLSLLEPLKSWAVEELKVVETPKKDVYARKYIVTGQL
NKVGYMTYIRRQALRRNLYGYAKKITKNEVEIYLISENQKSVEDFKKFVQKGSRKSIVKHIEDKEVEFSGKPTKVGFRII
S

Specific function: Catalyzes the ATP-dependent polymerization of arginine and aspartate to multi-L-arginyl-poly-L-aspartic acid (cyanophycin; a water-insoluble reserve polymer) [H]

COG id: COG1181

COG function: function code M; D-alanine-D-alanine ligase and related ATP-grasp enzymes

Gene ontology:

Cell location: Cytoplasmic

Metaboloic importance: NA

Operon status: Not Known

Operon components: None

Similarity: Contains 1 ATP-grasp domain [H]

Homologues:

None

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR011761
- InterPro:   IPR013651
- InterPro:   IPR013815
- InterPro:   IPR013816
- InterPro:   IPR011810
- InterPro:   IPR004101
- InterPro:   IPR013221 [H]

Pfam domain/function: PF02875 Mur_ligase_C; PF08245 Mur_ligase_M; PF08443 RimK [H]

EC number: =6.3.2.29; =6.3.2.30 [H]

Molecular weight: Translated: 54790; Mature: 54790

Theoretical pI: Translated: 9.24; Mature: 9.24

Prosite motif: PS50975 ATP_GRASP ; PS51160 ACYLPHOSPHATASE_3

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.6 %Cys     (Translated Protein)
0.8 %Met     (Translated Protein)
1.5 %Cys+Met (Translated Protein)
0.6 %Cys     (Mature Protein)
0.8 %Met     (Mature Protein)
1.5 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MKSQEVVLLPHLNKRVVKDITGFKLCSYLVALEGWRRGLNLTWYNDETSKCKLDRLNSST
CCCCCEEECCCCCHHHHHHHHHHHHHHHHHHHHHHHCCCCEEEECCCCCCEEHHHCCCCC
HGKFYSLSDGSKTHYFFRSRGDKVTNKAVRICQDKEETKSYLQKSNVPIPLGKVLEEDND
CCCEEEECCCCCEEEEEECCCCHHHHHHHHHHCCHHHHHHHHHHCCCCCCHHHHHCCCCH
IINYANEIDYPVIIKPLKGSMGKGVYTNINSEEELKGILKELRSKFSHKEYLVEKHYPGE
HHHHHHHCCCCEEEEECCCCCCCCEEECCCCHHHHHHHHHHHHHHHCHHHHHHHCCCCCC
EYRIYVVGDQVIGATNRKPANIIGDGKHTVQKLIDLKNEERKKNPYLSPKPIEADYEITF
CEEEEEEECCEECCCCCCCCCEECCCHHHHHHHHHCCCHHHHCCCCCCCCCCCCCCEEEE
MLERVGYDLNSVPEKGEQVILREKSNLSSGGDPIEATDELSPSVRQIAVDALKALPSIPH
EEHHHCCCCCCCCCCCCEEEEEECCCCCCCCCCCCCCHHCCHHHHHHHHHHHHHCCCCCC
GGVDIIVDPSDPSNGVVLEINATAEIGFHPFPLIGESKDVPAAIIDYYFPETKGKYRSPF
CCEEEEECCCCCCCCEEEEEECCEEECCCCCCCCCCCCCCCHHHHHHHCCCCCCCCCCCH
YFDYLSLLEPLKSWAVEELKVVETPKKDVYARKYIVTGQLNKVGYMTYIRRQALRRNLYG
HHHHHHHHHHHHHHHHHHHHHHCCCCHHHHEEEEEEECCCCHHHHHHHHHHHHHHHHHHH
YAKKITKNEVEIYLISENQKSVEDFKKFVQKGSRKSIVKHIEDKEVEFSGKPTKVGFRII
HHHHHCCCCEEEEEECCCCHHHHHHHHHHHCCCHHHHHHHHCCCCEEECCCCCEECEEEE
S
C
>Mature Secondary Structure
MKSQEVVLLPHLNKRVVKDITGFKLCSYLVALEGWRRGLNLTWYNDETSKCKLDRLNSST
CCCCCEEECCCCCHHHHHHHHHHHHHHHHHHHHHHHCCCCEEEECCCCCCEEHHHCCCCC
HGKFYSLSDGSKTHYFFRSRGDKVTNKAVRICQDKEETKSYLQKSNVPIPLGKVLEEDND
CCCEEEECCCCCEEEEEECCCCHHHHHHHHHHCCHHHHHHHHHHCCCCCCHHHHHCCCCH
IINYANEIDYPVIIKPLKGSMGKGVYTNINSEEELKGILKELRSKFSHKEYLVEKHYPGE
HHHHHHHCCCCEEEEECCCCCCCCEEECCCCHHHHHHHHHHHHHHHCHHHHHHHCCCCCC
EYRIYVVGDQVIGATNRKPANIIGDGKHTVQKLIDLKNEERKKNPYLSPKPIEADYEITF
CEEEEEEECCEECCCCCCCCCEECCCHHHHHHHHHCCCHHHHCCCCCCCCCCCCCCEEEE
MLERVGYDLNSVPEKGEQVILREKSNLSSGGDPIEATDELSPSVRQIAVDALKALPSIPH
EEHHHCCCCCCCCCCCCEEEEEECCCCCCCCCCCCCCHHCCHHHHHHHHHHHHHCCCCCC
GGVDIIVDPSDPSNGVVLEINATAEIGFHPFPLIGESKDVPAAIIDYYFPETKGKYRSPF
CCEEEEECCCCCCCCEEEEEECCEEECCCCCCCCCCCCCCCHHHHHHHCCCCCCCCCCCH
YFDYLSLLEPLKSWAVEELKVVETPKKDVYARKYIVTGQLNKVGYMTYIRRQALRRNLYG
HHHHHHHHHHHHHHHHHHHHHHCCCCHHHHEEEEEEECCCCHHHHHHHHHHHHHHHHHHH
YAKKITKNEVEIYLISENQKSVEDFKKFVQKGSRKSIVKHIEDKEVEFSGKPTKVGFRII
HHHHHCCCCEEEEEECCCCHHHHHHHHHHHCCCHHHHHHHHCCCCEEECCCCCEECEEEE
S
C

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: NA