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The map label for this gene is capD [H]

Identifier: 23100351

GI number: 23100351

Start: 2983957

End: 2985783

Strand: Reverse

Name: capD [H]

Synonym: OB2896

Alternate gene names: 23100351

Gene position: 2985783-2983957 (Counterclockwise)

Preceding gene: 23100352

Following gene: 23100350

Centisome position: 82.24

GC content: 39.24

Gene sequence:

>1827_bases
ATGTCATATAAGCAAAGATTAGCATTGCTTATTCTTCTTGATTCGGCAATTGTCAGCACGGCAATTTTTATCTCGTTTTG
GGTTGTTTATCCATCCTATCCGGAGATATTACTATTTGAAGAATTAGGCTTAAGTGCACTTGCGTTGCTCGTATTCCATC
ATATTTATGCATTTATTTATAAGTTATATAACAAAGTATGGGCATATGCGAGTGTAAGAGAATTATTAGCGATTGTACAA
GCAGTGACACTAACTGTTTTATCAGTTGCAGTTGTGCAATTAATCGCAAATGGAATGATTTACCGGAGAGCACTAGTAAT
CACATGGTTATTACATATTGTATTAATCGGTGGATCTCGATTTATGTGGAGAGTTGTCCGAGATCGCTATATTAAGCAGA
ATGCAAATCAAAAACGCACGCTTATTATTGGTGCAGGATCTGCAGGAGCAATGATTGCAAGGCAACTTCAGAATGAACAT
CAATCAGAAATCTACCCAGTTGCATTTATAGATGATGATATAGCGAAACAGAAGATGGAAGTATATAATCTTCCTGTTGT
TGGCAGAGCAAGTGACATTCCCGAAAAAGTAAAGGATTTCAAAATTGAACATATTGTAATTGCGATTCCATCATTAAGAA
ACGGTGAACTTAAGAGAATTGTTGAGCTTTGTGGAGAGACGGAAGCAAAGGTTCAAATGATACCGAAAATAGAAGACTTA
ATGACTGGTAAAGTATCGGTCAGTCATTTGAAGAATGTAGAAGTAGAGGATCTCTTAGGAAGAGAACCTGTGAAACTAGA
CATTCAAGCAATATCGGAATATGTGACAGAAAATACGGTTATGGTAACAGGTGCTGGCGGTTCGATTGGGTCAGAAGTTT
GTCGACAAGTGATGCGATTTAAACCTCAGAAGATTTTACTAGTTGGGCATGGAGAATTTAGCATTTATAACATTGATATG
GAATTAAGAACCAACTATAAGGATTCTGAGATTGAAATTGTCCCACTTATTGGCGATGTTCAAGACCGAGATCGCATGTA
TCAAATTATGCAAGAACATCATCCAGCAGTTGTGTATCATGCGGCTGCACATAAACATGTTCCACTGATGGAATATAACC
CGCATGAGGCGATTAAGAATAACATTATCGGTACGAAAAATGTCGCAGAAGCAGCCGATGCATTTGGCGTCAGCACATTC
GTCATGGTATCTACCGATAAAGCAGTGAACCCAACCAATGTAATGGGGGCAAGCAAACGGATAGCAGAAATGGTCGTACA
AGACTTAGCATCGCGGAGCAGTACGAAATTTGTCGCAGTGAGATTTGGAAATGTACTGGGTAGTCGAGGAAGTGTGATCC
CGCTCTTTAAGAAGCAGATGGAACAAGGGGGACCTATTACGGTTACTCACCCAGAGATGACTCGGTACTTTATGACTATT
CCAGAAGCATCTCGATTGGTAGTACAAGCAGGGACCTTGGCAAGAGGCGGAGAAATCTTTGTGCTTGATATGGGAAATCC
AGTGAAAATTGCCGACCTCGCTCGAAATTTGATTCGCTTATCTGGATATAACGAAGACGAAATTGAAATTAAATATACAG
GAATCCGACCTGGAGAGAAAATGTTCGAGGAATTACTAGGTGAGGATGAAGTGTTCCCTGAACAGGTGTATGAAAAGATA
TACATTGGAAAAACGGTAGAAGTAGACCAGCAATTGATTACGAACCTATTAATCCATTTTGAAGGGTTCAATACACAAAC
CTTAAAAGATGAAATGATGAATATTGTCTTTGCAGAGCGTCGAATTTTGGAGACGATGAAGGCTTAA

Upstream 100 bases:

>100_bases
GGTAGAATATTTAAATAATCTGATTTCTGACTCTTTTATCGATAAGGAAAATAATGAATATAGTGTTATCTTATAGTTTC
TTAGAATGGAGTGTACATAC

Downstream 100 bases:

>100_bases
ATTTTAAACAGTTCATATAAATTATGATTCATATGATGAGGGGTAAGAAATGACTATAAACACAGTAGAATTTAAAAATT
CATTAGCTGACAGATTAACT

Product: capsular polysaccharide biosynthesis

Products: dTDP-4-dehydro-6-deoxy-D-glucose; H2O [C]

Alternate protein names: NA

Number of amino acids: Translated: 608; Mature: 607

Protein sequence:

>608_residues
MSYKQRLALLILLDSAIVSTAIFISFWVVYPSYPEILLFEELGLSALALLVFHHIYAFIYKLYNKVWAYASVRELLAIVQ
AVTLTVLSVAVVQLIANGMIYRRALVITWLLHIVLIGGSRFMWRVVRDRYIKQNANQKRTLIIGAGSAGAMIARQLQNEH
QSEIYPVAFIDDDIAKQKMEVYNLPVVGRASDIPEKVKDFKIEHIVIAIPSLRNGELKRIVELCGETEAKVQMIPKIEDL
MTGKVSVSHLKNVEVEDLLGREPVKLDIQAISEYVTENTVMVTGAGGSIGSEVCRQVMRFKPQKILLVGHGEFSIYNIDM
ELRTNYKDSEIEIVPLIGDVQDRDRMYQIMQEHHPAVVYHAAAHKHVPLMEYNPHEAIKNNIIGTKNVAEAADAFGVSTF
VMVSTDKAVNPTNVMGASKRIAEMVVQDLASRSSTKFVAVRFGNVLGSRGSVIPLFKKQMEQGGPITVTHPEMTRYFMTI
PEASRLVVQAGTLARGGEIFVLDMGNPVKIADLARNLIRLSGYNEDEIEIKYTGIRPGEKMFEELLGEDEVFPEQVYEKI
YIGKTVEVDQQLITNLLIHFEGFNTQTLKDEMMNIVFAERRILETMKA

Sequences:

>Translated_608_residues
MSYKQRLALLILLDSAIVSTAIFISFWVVYPSYPEILLFEELGLSALALLVFHHIYAFIYKLYNKVWAYASVRELLAIVQ
AVTLTVLSVAVVQLIANGMIYRRALVITWLLHIVLIGGSRFMWRVVRDRYIKQNANQKRTLIIGAGSAGAMIARQLQNEH
QSEIYPVAFIDDDIAKQKMEVYNLPVVGRASDIPEKVKDFKIEHIVIAIPSLRNGELKRIVELCGETEAKVQMIPKIEDL
MTGKVSVSHLKNVEVEDLLGREPVKLDIQAISEYVTENTVMVTGAGGSIGSEVCRQVMRFKPQKILLVGHGEFSIYNIDM
ELRTNYKDSEIEIVPLIGDVQDRDRMYQIMQEHHPAVVYHAAAHKHVPLMEYNPHEAIKNNIIGTKNVAEAADAFGVSTF
VMVSTDKAVNPTNVMGASKRIAEMVVQDLASRSSTKFVAVRFGNVLGSRGSVIPLFKKQMEQGGPITVTHPEMTRYFMTI
PEASRLVVQAGTLARGGEIFVLDMGNPVKIADLARNLIRLSGYNEDEIEIKYTGIRPGEKMFEELLGEDEVFPEQVYEKI
YIGKTVEVDQQLITNLLIHFEGFNTQTLKDEMMNIVFAERRILETMKA
>Mature_607_residues
SYKQRLALLILLDSAIVSTAIFISFWVVYPSYPEILLFEELGLSALALLVFHHIYAFIYKLYNKVWAYASVRELLAIVQA
VTLTVLSVAVVQLIANGMIYRRALVITWLLHIVLIGGSRFMWRVVRDRYIKQNANQKRTLIIGAGSAGAMIARQLQNEHQ
SEIYPVAFIDDDIAKQKMEVYNLPVVGRASDIPEKVKDFKIEHIVIAIPSLRNGELKRIVELCGETEAKVQMIPKIEDLM
TGKVSVSHLKNVEVEDLLGREPVKLDIQAISEYVTENTVMVTGAGGSIGSEVCRQVMRFKPQKILLVGHGEFSIYNIDME
LRTNYKDSEIEIVPLIGDVQDRDRMYQIMQEHHPAVVYHAAAHKHVPLMEYNPHEAIKNNIIGTKNVAEAADAFGVSTFV
MVSTDKAVNPTNVMGASKRIAEMVVQDLASRSSTKFVAVRFGNVLGSRGSVIPLFKKQMEQGGPITVTHPEMTRYFMTIP
EASRLVVQAGTLARGGEIFVLDMGNPVKIADLARNLIRLSGYNEDEIEIKYTGIRPGEKMFEELLGEDEVFPEQVYEKIY
IGKTVEVDQQLITNLLIHFEGFNTQTLKDEMMNIVFAERRILETMKA

Specific function: Required for the biosynthesis of type 1 capsular polysaccharide [H]

COG id: COG1086

COG function: function code MG; Predicted nucleoside-diphosphate sugar epimerases

Gene ontology:

Cell location: Cell membrane; Multi-pass membrane protein (Potential) [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the polysaccharide synthase family [H]

Homologues:

None

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR016040
- InterPro:   IPR003869 [H]

Pfam domain/function: PF02719 Polysacc_synt_2 [H]

EC number: 4.2.1.46 [C]

Molecular weight: Translated: 68446; Mature: 68315

Theoretical pI: Translated: 6.58; Mature: 6.58

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.3 %Cys     (Translated Protein)
3.9 %Met     (Translated Protein)
4.3 %Cys+Met (Translated Protein)
0.3 %Cys     (Mature Protein)
3.8 %Met     (Mature Protein)
4.1 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MSYKQRLALLILLDSAIVSTAIFISFWVVYPSYPEILLFEELGLSALALLVFHHIYAFIY
CCHHHHEEHHHHHHHHHHHHHHHHHHHHHCCCCCCEEEEHHHCHHHHHHHHHHHHHHHHH
KLYNKVWAYASVRELLAIVQAVTLTVLSVAVVQLIANGMIYRRALVITWLLHIVLIGGSR
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCHHHHHHHHHHHHHHHHHHCCCH
FMWRVVRDRYIKQNANQKRTLIIGAGSAGAMIARQLQNEHQSEIYPVAFIDDDIAKQKME
HHHHHHHHHHHHCCCCCCEEEEEECCCHHHHHHHHHHHHHHCCEEEEEEECCHHHHHHHH
VYNLPVVGRASDIPEKVKDFKIEHIVIAIPSLRNGELKRIVELCGETEAKVQMIPKIEDL
HHCCCCCCCCCCCHHHHHHCEEEEEEEECCCCCCCHHHHHHHHHCCCCCCEEECCCHHHH
MTGKVSVSHLKNVEVEDLLGREPVKLDIQAISEYVTENTVMVTGAGGSIGSEVCRQVMRF
HCCCEEHHHHCCCCHHHHHCCCCCEEEHHHHHHHHCCCEEEEEECCCCHHHHHHHHHHHC
KPQKILLVGHGEFSIYNIDMELRTNYKDSEIEIVPLIGDVQDRDRMYQIMQEHHPAVVYH
CCCEEEEEECCCEEEEEEEEEEECCCCCCCEEEEEEECCCCHHHHHHHHHHHCCCEEEEE
AAAHKHVPLMEYNPHEAIKNNIIGTKNVAEAADAFGVSTFVMVSTDKAVNPTNVMGASKR
ECCCCCCCCEECCCHHHHHHCCCCCCHHHHHHHHCCCEEEEEEECCCCCCCHHHCCHHHH
IAEMVVQDLASRSSTKFVAVRFGNVLGSRGSVIPLFKKQMEQGGPITVTHPEMTRYFMTI
HHHHHHHHHHCCCCCEEEEEEECHHHCCCCCEEHHHHHHHHCCCCEEEECHHHHHHEEEC
PEASRLVVQAGTLARGGEIFVLDMGNPVKIADLARNLIRLSGYNEDEIEIKYTGIRPGEK
CCHHEEEEEECCCCCCCEEEEEECCCCEEHHHHHHHHHHHCCCCCCCEEEEEECCCCHHH
MFEELLGEDEVFPEQVYEKIYIGKTVEVDQQLITNLLIHFEGFNTQTLKDEMMNIVFAER
HHHHHCCCCCCCHHHHHHHHHCCCCCHHHHHHHHHHHHHCCCCCCHHHHHHHHHHHHHHH
RILETMKA
HHHHHHCC
>Mature Secondary Structure 
SYKQRLALLILLDSAIVSTAIFISFWVVYPSYPEILLFEELGLSALALLVFHHIYAFIY
CHHHHEEHHHHHHHHHHHHHHHHHHHHHCCCCCCEEEEHHHCHHHHHHHHHHHHHHHHH
KLYNKVWAYASVRELLAIVQAVTLTVLSVAVVQLIANGMIYRRALVITWLLHIVLIGGSR
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCHHHHHHHHHHHHHHHHHHCCCH
FMWRVVRDRYIKQNANQKRTLIIGAGSAGAMIARQLQNEHQSEIYPVAFIDDDIAKQKME
HHHHHHHHHHHHCCCCCCEEEEEECCCHHHHHHHHHHHHHHCCEEEEEEECCHHHHHHHH
VYNLPVVGRASDIPEKVKDFKIEHIVIAIPSLRNGELKRIVELCGETEAKVQMIPKIEDL
HHCCCCCCCCCCCHHHHHHCEEEEEEEECCCCCCCHHHHHHHHHCCCCCCEEECCCHHHH
MTGKVSVSHLKNVEVEDLLGREPVKLDIQAISEYVTENTVMVTGAGGSIGSEVCRQVMRF
HCCCEEHHHHCCCCHHHHHCCCCCEEEHHHHHHHHCCCEEEEEECCCCHHHHHHHHHHHC
KPQKILLVGHGEFSIYNIDMELRTNYKDSEIEIVPLIGDVQDRDRMYQIMQEHHPAVVYH
CCCEEEEEECCCEEEEEEEEEEECCCCCCCEEEEEEECCCCHHHHHHHHHHHCCCEEEEE
AAAHKHVPLMEYNPHEAIKNNIIGTKNVAEAADAFGVSTFVMVSTDKAVNPTNVMGASKR
ECCCCCCCCEECCCHHHHHHCCCCCCHHHHHHHHCCCEEEEEEECCCCCCCHHHCCHHHH
IAEMVVQDLASRSSTKFVAVRFGNVLGSRGSVIPLFKKQMEQGGPITVTHPEMTRYFMTI
HHHHHHHHHHCCCCCEEEEEEECHHHCCCCCEEHHHHHHHHCCCCEEEECHHHHHHEEEC
PEASRLVVQAGTLARGGEIFVLDMGNPVKIADLARNLIRLSGYNEDEIEIKYTGIRPGEK
CCHHEEEEEECCCCCCCEEEEEECCCCEEHHHHHHHHHHHCCCCCCCEEEEEECCCCHHH
MFEELLGEDEVFPEQVYEKIYIGKTVEVDQQLITNLLIHFEGFNTQTLKDEMMNIVFAER
HHHHHCCCCCCCHHHHHHHHHCCCCCHHHHHHHHHHHHHCCCCCCHHHHHHHHHHHHHHH
RILETMKA
HHHHHHCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NAD. [C]

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): 0.093 {dTDPglucose}} [C]

Substrates: dTDPglucose [C]

Specific reaction: dTDPglucose --> dTDP-4-dehydro-6-deoxy-D-glucose + H2O [C]

General reaction: Elimination (of H2O C-O bond cleavage [C]

Inhibitor: p-Chloromercuribenzoate; TMP [C]

Structure determination priority: 6.0

TargetDB status: NA

Availability: NA

References: 7961465 [H]