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The map label for this gene is bphC [H]

Identifier: 23100323

GI number: 23100323

Start: 2952654

End: 2953637

Strand: Reverse

Name: bphC [H]

Synonym: OB2868

Alternate gene names: 23100323

Gene position: 2953637-2952654 (Counterclockwise)

Preceding gene: 23100324

Following gene: 23100322

Centisome position: 81.36

GC content: 39.63

Gene sequence:

>984_bases
ATGAACTTTGATATTAAACGTACTGGACGTGCTGTACTTCATGTTACAGATTTAGACAAATCAAGAGAGTTTTATGATGC
ACTTGGCTTAATCGAAACGGAATCAGATGATGAGAACATTTTCTATCGCGCAATTGAAGATCATAATCATCATTGCCTTT
GGTTAAAAAAGAAACCAGAAGCAGCTGTAGAAGTAATTAGCTATCGAGTCAATGCAGACGAAGACCTTGAGGAATTAGCA
GCATTCTTTCAGAGTCAAGGAACAGAAGTAAAGTGGATGGAAAAAGGTACCCAAAAAGGAATTGGACGTACCATTCGCAT
TCAAGATATTTCTGGAATGCCTGTTGAATTCTATTTTGAAATGGATAAAGTAGAGCGCATGCTGCAGCGTTTTGACATTC
ATGTAGGTGCAAAAGTGCAGCGTATCGATCACTTCAACTGTGCAGTACCTGATGTACAAAAAGCTTATGACTATTATATA
AAAGAGCTTGGCTTTGCTTGCTCAGAGTACACTACTTCTAAAGGAGAAGATAATATTTGGGCAGCATGGTTACACCGAAA
ACCTGGCGTGCATGACACAGCTTTCATGAACAGTGTAGGACCACGACTTCACCATATTGGTTTCTGGTTAAAAGACCCAA
TGAGCCTAATCGATGGATGTGACTACCTAGCTGCAAAAGGATTATCTAAAACAATTGAGCGAGGACCAGGACGCCATGGG
TTGTCTAATGCATTCTTCTTATATTTAAGAGATCCAGACGGACACCGTATCGAATTGTATAATGGCGATTATTACACTGG
GGATTCTGATTTTGATCCAATTGAATGGGATTTAGATGATCCTCAACGTCAAACTTTCTGGGGACATGAAGCGCCAGATA
GTTGGTTTGACGAGGCTTCGACATTCCTGAATGTTTATACAGGGGAAAAAATTGACACCAAACAGCCAACATTAGAAAAA
AGAAAACCAAGCACCGTAATATAA

Upstream 100 bases:

>100_bases
TTGAATTCTATACCGAGCAAAAAATTATCCATGTAGCCATTGGTGATCATCATATACCGCAGTTTGGAAAGTAATGTCTA
AAATGGAGAGGGGAAACAAG

Downstream 100 bases:

>100_bases
ATAATCTATATATTTAGCAATCTAACAGGAGGTTATATATAAAATGAGTACGAAATACGATGCTATAAAAAAAAGATTGA
GAGGTTCAATCGCACCGGTA

Product: 3,4-dihydroxyphenylacetate 2,3-dioxygenase

Products: NA

Alternate protein names: Mn(II)-dependent 2,3-dihydroxybiphenyl 1,2-dioxygenase; Mn(II)-dependent 23OHBP oxygenase [H]

Number of amino acids: Translated: 327; Mature: 327

Protein sequence:

>327_residues
MNFDIKRTGRAVLHVTDLDKSREFYDALGLIETESDDENIFYRAIEDHNHHCLWLKKKPEAAVEVISYRVNADEDLEELA
AFFQSQGTEVKWMEKGTQKGIGRTIRIQDISGMPVEFYFEMDKVERMLQRFDIHVGAKVQRIDHFNCAVPDVQKAYDYYI
KELGFACSEYTTSKGEDNIWAAWLHRKPGVHDTAFMNSVGPRLHHIGFWLKDPMSLIDGCDYLAAKGLSKTIERGPGRHG
LSNAFFLYLRDPDGHRIELYNGDYYTGDSDFDPIEWDLDDPQRQTFWGHEAPDSWFDEASTFLNVYTGEKIDTKQPTLEK
RKPSTVI

Sequences:

>Translated_327_residues
MNFDIKRTGRAVLHVTDLDKSREFYDALGLIETESDDENIFYRAIEDHNHHCLWLKKKPEAAVEVISYRVNADEDLEELA
AFFQSQGTEVKWMEKGTQKGIGRTIRIQDISGMPVEFYFEMDKVERMLQRFDIHVGAKVQRIDHFNCAVPDVQKAYDYYI
KELGFACSEYTTSKGEDNIWAAWLHRKPGVHDTAFMNSVGPRLHHIGFWLKDPMSLIDGCDYLAAKGLSKTIERGPGRHG
LSNAFFLYLRDPDGHRIELYNGDYYTGDSDFDPIEWDLDDPQRQTFWGHEAPDSWFDEASTFLNVYTGEKIDTKQPTLEK
RKPSTVI
>Mature_327_residues
MNFDIKRTGRAVLHVTDLDKSREFYDALGLIETESDDENIFYRAIEDHNHHCLWLKKKPEAAVEVISYRVNADEDLEELA
AFFQSQGTEVKWMEKGTQKGIGRTIRIQDISGMPVEFYFEMDKVERMLQRFDIHVGAKVQRIDHFNCAVPDVQKAYDYYI
KELGFACSEYTTSKGEDNIWAAWLHRKPGVHDTAFMNSVGPRLHHIGFWLKDPMSLIDGCDYLAAKGLSKTIERGPGRHG
LSNAFFLYLRDPDGHRIELYNGDYYTGDSDFDPIEWDLDDPQRQTFWGHEAPDSWFDEASTFLNVYTGEKIDTKQPTLEK
RKPSTVI

Specific function: Catalyzes the meta-cleavage of the hydroxylated biphenyl ring. The enzyme can oxidize a wide range of substrates, and the substrate preference order is 2,3-dihydroxybiphenyl > 3- methylcatechol > catechol > 4-methylcatechol > 4-chlorocatechol [H]

COG id: NA

COG function: NA

Gene ontology:

Cell location: Cytoplasmic

Metaboloic importance: NA

Operon status: Not Known

Operon components: None

Similarity: Belongs to the extradiol ring-cleavage dioxygenase family [H]

Homologues:

None

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR004360 [H]

Pfam domain/function: PF00903 Glyoxalase [H]

EC number: =1.13.11.39 [H]

Molecular weight: Translated: 37865; Mature: 37865

Theoretical pI: Translated: 4.98; Mature: 4.98

Prosite motif: PS00082 EXTRADIOL_DIOXYGENAS

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.2 %Cys     (Translated Protein)
2.1 %Met     (Translated Protein)
3.4 %Cys+Met (Translated Protein)
1.2 %Cys     (Mature Protein)
2.1 %Met     (Mature Protein)
3.4 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MNFDIKRTGRAVLHVTDLDKSREFYDALGLIETESDDENIFYRAIEDHNHHCLWLKKKPE
CCCCCCCCCCEEEEEECCCCHHHHHHHHCCEECCCCCCCEEEEEEECCCCEEEEEECCCH
AAVEVISYRVNADEDLEELAAFFQSQGTEVKWMEKGTQKGIGRTIRIQDISGMPVEFYFE
HHHHHHHEECCCCCCHHHHHHHHHCCCCEEEEHHHHHHHCCCCEEEEEECCCCCEEEEEE
MDKVERMLQRFDIHVGAKVQRIDHFNCAVPDVQKAYDYYIKELGFACSEYTTSKGEDNIW
HHHHHHHHHHHCCCCCCEEEEECCCCCCCCHHHHHHHHHHHHHCCHHHHHCCCCCCCCEE
AAWLHRKPGVHDTAFMNSVGPRLHHIGFWLKDPMSLIDGCDYLAAKGLSKTIERGPGRHG
EEEECCCCCCCHHHHHHHCCCHHHHHHHHHCCHHHHHCCCHHHHHHHHHHHHHCCCCCCC
LSNAFFLYLRDPDGHRIELYNGDYYTGDSDFDPIEWDLDDPQRQTFWGHEAPDSWFDEAS
CCCEEEEEEECCCCCEEEEECCCEECCCCCCCCEECCCCCCHHHCCCCCCCCCHHHHHHH
TFLNVYTGEKIDTKQPTLEKRKPSTVI
HEEEEECCCCCCCCCCCHHHCCCCCCC
>Mature Secondary Structure
MNFDIKRTGRAVLHVTDLDKSREFYDALGLIETESDDENIFYRAIEDHNHHCLWLKKKPE
CCCCCCCCCCEEEEEECCCCHHHHHHHHCCEECCCCCCCEEEEEEECCCCEEEEEECCCH
AAVEVISYRVNADEDLEELAAFFQSQGTEVKWMEKGTQKGIGRTIRIQDISGMPVEFYFE
HHHHHHHEECCCCCCHHHHHHHHHCCCCEEEEHHHHHHHCCCCEEEEEECCCCCEEEEEE
MDKVERMLQRFDIHVGAKVQRIDHFNCAVPDVQKAYDYYIKELGFACSEYTTSKGEDNIW
HHHHHHHHHHHCCCCCCEEEEECCCCCCCCHHHHHHHHHHHHHCCHHHHHCCCCCCCCEE
AAWLHRKPGVHDTAFMNSVGPRLHHIGFWLKDPMSLIDGCDYLAAKGLSKTIERGPGRHG
EEEECCCCCCCHHHHHHHCCCHHHHHHHHHCCHHHHHCCCHHHHHHHHHHHHHCCCCCCC
LSNAFFLYLRDPDGHRIELYNGDYYTGDSDFDPIEWDLDDPQRQTFWGHEAPDSWFDEAS
CCCEEEEEEECCCCCEEEEECCCEECCCCCCCCEECCCCCCHHHCCCCCCCCCHHHHHHH
TFLNVYTGEKIDTKQPTLEKRKPSTVI
HEEEEECCCCCCCCCCCHHHCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 12672826 [H]