Click here to switch to the map view.

The map label for this gene is yiaO [H]

Identifier: 23100317

GI number: 23100317

Start: 2946835

End: 2947908

Strand: Reverse

Name: yiaO [H]

Synonym: OB2862

Alternate gene names: 23100317

Gene position: 2947908-2946835 (Counterclockwise)

Preceding gene: 23100318

Following gene: 23100316

Centisome position: 81.2

GC content: 38.64

Gene sequence:

>1074_bases
ATGCAAAACAGTTACATCAGAAATTGTGTTCTCTTAATCATGTCTTTATTTATCATATTGGTTTTAACATCCTGTGCCAA
TAAACCATCATCCAAACCAGGGGATTCAGTCGAGGAAAACCATAACCTAATAGTTTCTCACTTTCAGCCTGGAAATCATC
CAATTCAAACCAAAATCCTTGATGGACTTGCTGATGATTTGAATGAACAAACAGATGGAAGCATAACAATGGAATTTTAT
CCATCCAATACACTCGGTGATGCCGGATCACAATATGATATGGCTGTAACAAGAGAAGCAGATATTGCTTTAAGTGTACA
TGGTTACTCGCCTGGAAGATTCCCACTTGTGACAATTATGGAACTACCATTTCTAGCGGAATCTGCGGAACATGCTTCGG
AAATTCTAATGACCTTATATGAAGAGTTTCCGGAAATGCAGGAGGAGCATGCAGATACGTATCCATTATTTTTGTTCTCG
GCTGAACCTGCCCAGATTATAAGTAAAGATTTTAGAATTGAAACACCTGAAGATATAAAAGGTTTACGTGTACGATCCCC
ATCTCCAATGGCAAATAATATTATTGAGACGTTAGGAGCGACACCAGTCTCTATGCCGATGGGAGATGTTTATGAATCGT
TAGAAAGAGGTGTAATCGATGCTGCAGTCGTACCTTTTGAATCCCTTTATAATTATAGTTTCCATGAGGTAACAAATTAT
ATTACAATGGGTGATTTCTCTGCTACACCATTTTTTAGTGTGATGAGCAAGAAAACCTATGAAAATTTCAGTGACGACGA
TCAATTCGTACTAGATAATCTAATAGGGAAAGAACTGGCAAAAGAAGCTGGAAGTATCTATGATGTGGACGGGAAAAAAG
GCTTTGAAACAGCAATTGAAAGCGGGGCAGAGGTGATTGAATTAGAAGGAGACGCTTTAATTCAATGGAAAAAAGCATTA
GAACCGGTGGTTAATGAGTGGATTGAAGAAAGAACAGCAGAAGGCTACCCTGCACAAGAAATCTATGACAGGGCAATGGA
ATTGAAAGAGGAACTCAGTGATAGAGAGGAGTAG

Upstream 100 bases:

>100_bases
CTTTAAATTGTAAGCGATTACTATAAAGTGTTTGCTAACTAGATAAAAGAGTGAACGATTAAATTGAGAGATGTGATTGA
TGAAGGAAAGGAGAAAACTT

Downstream 100 bases:

>100_bases
TAAGGTGGAGAGGTTTATATCAAAATTAAATGTAGGGTTACTTTATATTGCCCAAGCTGTTTTAATTATTATGGTATTCT
TAGTCACCGCAGATGTATTA

Product: hypothetical protein

Products: NA

Alternate protein names: 2,3-DKG-binding protein; Extracytoplasmic solute receptor protein yiaO [H]

Number of amino acids: Translated: 357; Mature: 357

Protein sequence:

>357_residues
MQNSYIRNCVLLIMSLFIILVLTSCANKPSSKPGDSVEENHNLIVSHFQPGNHPIQTKILDGLADDLNEQTDGSITMEFY
PSNTLGDAGSQYDMAVTREADIALSVHGYSPGRFPLVTIMELPFLAESAEHASEILMTLYEEFPEMQEEHADTYPLFLFS
AEPAQIISKDFRIETPEDIKGLRVRSPSPMANNIIETLGATPVSMPMGDVYESLERGVIDAAVVPFESLYNYSFHEVTNY
ITMGDFSATPFFSVMSKKTYENFSDDDQFVLDNLIGKELAKEAGSIYDVDGKKGFETAIESGAEVIELEGDALIQWKKAL
EPVVNEWIEERTAEGYPAQEIYDRAMELKEELSDREE

Sequences:

>Translated_357_residues
MQNSYIRNCVLLIMSLFIILVLTSCANKPSSKPGDSVEENHNLIVSHFQPGNHPIQTKILDGLADDLNEQTDGSITMEFY
PSNTLGDAGSQYDMAVTREADIALSVHGYSPGRFPLVTIMELPFLAESAEHASEILMTLYEEFPEMQEEHADTYPLFLFS
AEPAQIISKDFRIETPEDIKGLRVRSPSPMANNIIETLGATPVSMPMGDVYESLERGVIDAAVVPFESLYNYSFHEVTNY
ITMGDFSATPFFSVMSKKTYENFSDDDQFVLDNLIGKELAKEAGSIYDVDGKKGFETAIESGAEVIELEGDALIQWKKAL
EPVVNEWIEERTAEGYPAQEIYDRAMELKEELSDREE
>Mature_357_residues
MQNSYIRNCVLLIMSLFIILVLTSCANKPSSKPGDSVEENHNLIVSHFQPGNHPIQTKILDGLADDLNEQTDGSITMEFY
PSNTLGDAGSQYDMAVTREADIALSVHGYSPGRFPLVTIMELPFLAESAEHASEILMTLYEEFPEMQEEHADTYPLFLFS
AEPAQIISKDFRIETPEDIKGLRVRSPSPMANNIIETLGATPVSMPMGDVYESLERGVIDAAVVPFESLYNYSFHEVTNY
ITMGDFSATPFFSVMSKKTYENFSDDDQFVLDNLIGKELAKEAGSIYDVDGKKGFETAIESGAEVIELEGDALIQWKKAL
EPVVNEWIEERTAEGYPAQEIYDRAMELKEELSDREE

Specific function: Part of the tripartite ATP-independent periplasmic (TRAP) transport system yiaMNO involved in the uptake of 2,3- diketo-L-gulonate. This protein specifically binds 2,3-diketo-L- gulonate. Is not able to bind either L-ascorbate or dehydroascorbate [H]

COG id: COG1638

COG function: function code G; TRAP-type C4-dicarboxylate transport system, periplasmic component

Gene ontology:

Cell location: Periplasm [H]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the bacterial solute-binding protein 7 family [H]

Homologues:

Organism=Escherichia coli, GI1790004, Length=247, Percent_Identity=26.3157894736842, Blast_Score=90, Evalue=2e-19,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR018389
- InterPro:   IPR004682 [H]

Pfam domain/function: PF03480 SBP_bac_7 [H]

EC number: NA

Molecular weight: Translated: 39935; Mature: 39935

Theoretical pI: Translated: 4.01; Mature: 4.01

Prosite motif: PS00013 PROKAR_LIPOPROTEIN

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.6 %Cys     (Translated Protein)
3.6 %Met     (Translated Protein)
4.2 %Cys+Met (Translated Protein)
0.6 %Cys     (Mature Protein)
3.6 %Met     (Mature Protein)
4.2 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MQNSYIRNCVLLIMSLFIILVLTSCANKPSSKPGDSVEENHNLIVSHFQPGNHPIQTKIL
CCCHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCCCCCCCEEEEEECCCCCCHHHHHH
DGLADDLNEQTDGSITMEFYPSNTLGDAGSQYDMAVTREADIALSVHGYSPGRFPLVTIM
HHHHHHHCCCCCCCEEEEEECCCCCCCCCCCCCEEEEECCCEEEEEECCCCCCCHHHHHH
ELPFLAESAEHASEILMTLYEEFPEMQEEHADTYPLFLFSAEPAQIISKDFRIETPEDIK
HCCHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCEEEEECCCHHHHHHHCCCCCCCHHCC
GLRVRSPSPMANNIIETLGATPVSMPMGDVYESLERGVIDAAVVPFESLYNYSFHEVTNY
CCEECCCCCHHHHHHHHHCCCCCCCCHHHHHHHHHCCCHHHHHHCHHHHHCCCHHHHHCE
ITMGDFSATPFFSVMSKKTYENFSDDDQFVLDNLIGKELAKEAGSIYDVDGKKGFETAIE
EEECCCCCCHHHHHHHHHHHCCCCCCHHHHHHHHHHHHHHHHCCCEEECCCCCHHHHHHH
SGAEVIELEGDALIQWKKALEPVVNEWIEERTAEGYPAQEIYDRAMELKEELSDREE
CCCEEEEECCCHHHHHHHHHHHHHHHHHHHHCCCCCCHHHHHHHHHHHHHHHHCCCC
>Mature Secondary Structure
MQNSYIRNCVLLIMSLFIILVLTSCANKPSSKPGDSVEENHNLIVSHFQPGNHPIQTKIL
CCCHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCCCCCCCEEEEEECCCCCCHHHHHH
DGLADDLNEQTDGSITMEFYPSNTLGDAGSQYDMAVTREADIALSVHGYSPGRFPLVTIM
HHHHHHHCCCCCCCEEEEEECCCCCCCCCCCCCEEEEECCCEEEEEECCCCCCCHHHHHH
ELPFLAESAEHASEILMTLYEEFPEMQEEHADTYPLFLFSAEPAQIISKDFRIETPEDIK
HCCHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCEEEEECCCHHHHHHHCCCCCCCHHCC
GLRVRSPSPMANNIIETLGATPVSMPMGDVYESLERGVIDAAVVPFESLYNYSFHEVTNY
CCEECCCCCHHHHHHHHHCCCCCCCCHHHHHHHHHCCCHHHHHHCHHHHHCCCHHHHHCE
ITMGDFSATPFFSVMSKKTYENFSDDDQFVLDNLIGKELAKEAGSIYDVDGKKGFETAIE
EEECCCCCCHHHHHHHHHHHCCCCCCHHHHHHHHHHHHHHHHCCCEEECCCCCHHHHHHH
SGAEVIELEGDALIQWKKALEPVVNEWIEERTAEGYPAQEIYDRAMELKEELSDREE
CCCEEEEECCCHHHHHHHHHHHHHHHHHHHHCCCCCCHHHHHHHHHHHHHHHHCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 8041620; 9278503 [H]