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The map label for this gene is azoB [H]

Identifier: 23100308

GI number: 23100308

Start: 2937741

End: 2938586

Strand: Direct

Name: azoB [H]

Synonym: OB2853

Alternate gene names: 23100308

Gene position: 2937741-2938586 (Clockwise)

Preceding gene: 23100290

Following gene: 23100309

Centisome position: 80.92

GC content: 34.16

Gene sequence:

>846_bases
ATGAAAATTTTAGTAACAGGTTTCACTGGAAAAGTTGGTCTACAAGTTGCTAACTATTTAAATGCTAAAGATATTCCGAT
AGTATGTGCGGTTCGGAATGTTGAAAAGGCCAAAGCCAAATTTGGTGATGAATATGAATACGTCAACTTGGATTTTTCAG
ATTCAAGCACGTTTGATAATGCGCTCGAAAATGTAGATCGTATTTTTTTAATGTACCCACCAGGTGAAAACCTCCTATTT
GATACCTTTCTAGAAAAAGCCAAAGATAAAGGAATTAAACACATTACCTATTTATCGCTAAAAGACGTGCAATTCATGCC
ATTTATCCACCATTATAAAATGGAAAAGCTAATAAAAAGCAGCAAGATACCTTATACATTTGTTCGCGCTGGTTATTTCA
TGCAAAATTTAAATGACTTTCTTCGCAAGGAAATCCAAGAAAGACAGCGTATCTTTGTTCCAGCAGGCAAAGGAAAAACA
AGCTTTGTTGACACAAGAGATCTTGCTGAAGTCTCTGCATTATCTTTGATTGATGAAGTGAAGCATAGAAACCAAATTTA
TGTCATCACAGGAAATAAAGCCCTTGATTTTTTTGAGGTTGCAAATATAATGACTGAAATTATTGGAGTGAAAATTGAAT
ACACAAACCCTTCAGTTAAAGAGTTTAATAACTATATGAGTGAAATAGGCGTAGAAGAAGGATTTGTGAATGTTGTCGTT
GGTGTCCATATGCCAACAAAACTTGGACTTGCAAAAGGAATCAAGCATGATTATGAGAAAGTAACAGGCAAAAAGCCTAC
AAATATTGGACAATACATTATGGATTTCAAAGAGAATTGGATTTAG

Upstream 100 bases:

>100_bases
AATTTTGAATAATAACAAACAGCTAGTATGTGATTGAACACACTGTATTTTTAAAATCAAATACTTGTTATGAAATAGAA
TGTTTTGTGAGGAGTCAGTA

Downstream 100 bases:

>100_bases
GAAGGTTTACAGGAAATTCAATAATCGGGCGTCTTATATGGAAAAATCAAGGGTGCAACACTAGGTTGCACCTTGTTGCA
CCTATAACTATCTTTACTAT

Product: hypothetical protein

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 281; Mature: 281

Protein sequence:

>281_residues
MKILVTGFTGKVGLQVANYLNAKDIPIVCAVRNVEKAKAKFGDEYEYVNLDFSDSSTFDNALENVDRIFLMYPPGENLLF
DTFLEKAKDKGIKHITYLSLKDVQFMPFIHHYKMEKLIKSSKIPYTFVRAGYFMQNLNDFLRKEIQERQRIFVPAGKGKT
SFVDTRDLAEVSALSLIDEVKHRNQIYVITGNKALDFFEVANIMTEIIGVKIEYTNPSVKEFNNYMSEIGVEEGFVNVVV
GVHMPTKLGLAKGIKHDYEKVTGKKPTNIGQYIMDFKENWI

Sequences:

>Translated_281_residues
MKILVTGFTGKVGLQVANYLNAKDIPIVCAVRNVEKAKAKFGDEYEYVNLDFSDSSTFDNALENVDRIFLMYPPGENLLF
DTFLEKAKDKGIKHITYLSLKDVQFMPFIHHYKMEKLIKSSKIPYTFVRAGYFMQNLNDFLRKEIQERQRIFVPAGKGKT
SFVDTRDLAEVSALSLIDEVKHRNQIYVITGNKALDFFEVANIMTEIIGVKIEYTNPSVKEFNNYMSEIGVEEGFVNVVV
GVHMPTKLGLAKGIKHDYEKVTGKKPTNIGQYIMDFKENWI
>Mature_281_residues
MKILVTGFTGKVGLQVANYLNAKDIPIVCAVRNVEKAKAKFGDEYEYVNLDFSDSSTFDNALENVDRIFLMYPPGENLLF
DTFLEKAKDKGIKHITYLSLKDVQFMPFIHHYKMEKLIKSSKIPYTFVRAGYFMQNLNDFLRKEIQERQRIFVPAGKGKT
SFVDTRDLAEVSALSLIDEVKHRNQIYVITGNKALDFFEVANIMTEIIGVKIEYTNPSVKEFNNYMSEIGVEEGFVNVVV
GVHMPTKLGLAKGIKHDYEKVTGKKPTNIGQYIMDFKENWI

Specific function: Catalyzes the reductive cleavage of azo bond in aromatic azo compounds to the corresponding amines. Uses preferentially NADPH rather than NADH as an electron donor for its activity. The enzyme reductively cleaved Orange II and carboxy-Orange II, and can a

COG id: COG0702

COG function: function code MG; Predicted nucleoside-diphosphate-sugar epimerases

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the NmrA-type oxidoreductase family. Azoreductase type 3 subfamily [H]

Homologues:

Organism=Escherichia coli, GI1790656, Length=277, Percent_Identity=25.9927797833935, Blast_Score=83, Evalue=2e-17,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR016040
- InterPro:   IPR008030 [H]

Pfam domain/function: PF05368 NmrA [H]

EC number: NA

Molecular weight: Translated: 32184; Mature: 32184

Theoretical pI: Translated: 8.34; Mature: 8.34

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.4 %Cys     (Translated Protein)
3.2 %Met     (Translated Protein)
3.6 %Cys+Met (Translated Protein)
0.4 %Cys     (Mature Protein)
3.2 %Met     (Mature Protein)
3.6 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MKILVTGFTGKVGLQVANYLNAKDIPIVCAVRNVEKAKAKFGDEYEYVNLDFSDSSTFDN
CEEEEEECCCHHHHHHHHHCCCCCCEEEEEECCHHHHHHHCCCCEEEEEEECCCCCHHHH
ALENVDRIFLMYPPGENLLFDTFLEKAKDKGIKHITYLSLKDVQFMPFIHHYKMEKLIKS
HHHHHCEEEEEECCCCCHHHHHHHHHHHHCCCCEEEEEEECCCCHHHHHHHHHHHHHHHH
SKIPYTFVRAGYFMQNLNDFLRKEIQERQRIFVPAGKGKTSFVDTRDLAEVSALSLIDEV
CCCCCHHHHHHHHHHHHHHHHHHHHHHHHEEEEECCCCCCCCCCCHHHHHHHHHHHHHHH
KHRNQIYVITGNKALDFFEVANIMTEIIGVKIEYTNPSVKEFNNYMSEIGVEEGFVNVVV
HHCCEEEEEECCCCHHHHHHHHHHHHHHCEEEEECCCCHHHHHHHHHHCCCCCCEEEEEE
GVHMPTKLGLAKGIKHDYEKVTGKKPTNIGQYIMDFKENWI
EECCCHHHHHHHHHHHHHHHHCCCCCCCHHHHHHHHHHCCC
>Mature Secondary Structure
MKILVTGFTGKVGLQVANYLNAKDIPIVCAVRNVEKAKAKFGDEYEYVNLDFSDSSTFDN
CEEEEEECCCHHHHHHHHHCCCCCCEEEEEECCHHHHHHHCCCCEEEEEEECCCCCHHHH
ALENVDRIFLMYPPGENLLFDTFLEKAKDKGIKHITYLSLKDVQFMPFIHHYKMEKLIKS
HHHHHCEEEEEECCCCCHHHHHHHHHHHHCCCCEEEEEEECCCCHHHHHHHHHHHHHHHH
SKIPYTFVRAGYFMQNLNDFLRKEIQERQRIFVPAGKGKTSFVDTRDLAEVSALSLIDEV
CCCCCHHHHHHHHHHHHHHHHHHHHHHHHEEEEECCCCCCCCCCCHHHHHHHHHHHHHHH
KHRNQIYVITGNKALDFFEVANIMTEIIGVKIEYTNPSVKEFNNYMSEIGVEEGFVNVVV
HHCCEEEEEECCCCHHHHHHHHHHHHHHCEEEEECCCCHHHHHHHHHHCCCCCCEEEEEE
GVHMPTKLGLAKGIKHDYEKVTGKKPTNIGQYIMDFKENWI
EECCCHHHHHHHHHHHHHHHHCCCCCCCHHHHHHHHHHCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 12147495 [H]