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The map label for this gene is gatD [H]

Identifier: 23100205

GI number: 23100205

Start: 2820998

End: 2822047

Strand: Reverse

Name: gatD [H]

Synonym: OB2750

Alternate gene names: 23100205

Gene position: 2822047-2820998 (Counterclockwise)

Preceding gene: 23100206

Following gene: 23100204

Centisome position: 77.73

GC content: 40.67

Gene sequence:

>1050_bases
TTGGAAGCTTTGAATTTATATGGTATAGAGGATTTGCGTTACGAAGATACACCAAAGCCTATGATTGAAAAAGATGATGA
TGTCATTATTAAAGTAAAATCGGTAGGCATTTGTGGCTCGGATACTTCTCGATATAAAAAGCTAGGACCTTACGTGGAAG
GAATGACATTCGGTCACGAGTTTGCAGGTGAGGTAATAGAAGTAGGAAAAAGTGTCACAAATTTTAAATCAGGGGATCGA
GTTGTGGCCTGCCCTACTTTTTCATGTGGGAACTGTCATTACTGTAGGGAAGGACACCCAACTAGATGTGTGGAACTAAC
TGTAATTGGAGCAAGAAATCCAGGTGCTTATGCTGAGTATACGAAACTACCAGAAGGGCATGTGAAGATTTTGCCAGATT
CGATTGATGATGATACAGCAGCTCTGGTTGAGCCTTCCGCTGTAGTTGCGCATGGTTTTTACCGAGCATCAATTACACCA
GGTAGTTCGGTAGCGATCATGGGTGTCGGGAGTATAGGATTGTTAGCAGTTCAATGGGCTAAAATCTTCGGCGCTAGTAA
AGTGATTGCCATAGATATTGATGATCATAAATTAAAAATTGCTCAAGAACTTGGTGCGGATATTGTGGTAAATCCAATGA
AAGAAAATGCTGAGGGAATTGTAAAGCAACATACCGATGAGCTTGGTGTTGACCTTGCTGTGGAATCAGCCGGATCACCT
ATCACAAGTGAGCAAGTACTTGCTTTACCAAAAAAAGGTGGAGAAGTGGTTTTTCTTGGCATTCCTTATGGTGATATTAC
ACTAAAACGATATTATTTCGAAAAAATTGTTCGGAATGAACTAAGAATTCTTGGTTCATGGAATGCGTTGTCGGCACCTT
TTCCAGGGAAAGAGTGGAATGCAACTTTACATTACATGAGCACCGGCCAGTTAAATATAAAACCGATGATTTCTCATCGA
CTCGGACTGCAAGCTGGACCAGAAATCTTCCATCAAATTACCAATAAACTCTCCGATGCGGTAAAAGTAATCTTTCATCC
CGGTAAATAA

Upstream 100 bases:

>100_bases
TCAACTCAATCGTTGTTGGGATAGCAGCATACTTTCTAATCAAAAATAAAAAGAACTCAACCAAAGAATCGAATAACTAG
ATATAGAGAGGAGCTTTATC

Downstream 100 bases:

>100_bases
GGAACGAAGAGACTGGAGAATAACAACAAAAGTCAAGCGCCCGTTTTAAGGAAAGCCGACTAAGAACGCCTCGTCCTGTG
CCAACGTCGATATGACCCGG

Product: sorbitol dehydrogenase

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 349; Mature: 349

Protein sequence:

>349_residues
MEALNLYGIEDLRYEDTPKPMIEKDDDVIIKVKSVGICGSDTSRYKKLGPYVEGMTFGHEFAGEVIEVGKSVTNFKSGDR
VVACPTFSCGNCHYCREGHPTRCVELTVIGARNPGAYAEYTKLPEGHVKILPDSIDDDTAALVEPSAVVAHGFYRASITP
GSSVAIMGVGSIGLLAVQWAKIFGASKVIAIDIDDHKLKIAQELGADIVVNPMKENAEGIVKQHTDELGVDLAVESAGSP
ITSEQVLALPKKGGEVVFLGIPYGDITLKRYYFEKIVRNELRILGSWNALSAPFPGKEWNATLHYMSTGQLNIKPMISHR
LGLQAGPEIFHQITNKLSDAVKVIFHPGK

Sequences:

>Translated_349_residues
MEALNLYGIEDLRYEDTPKPMIEKDDDVIIKVKSVGICGSDTSRYKKLGPYVEGMTFGHEFAGEVIEVGKSVTNFKSGDR
VVACPTFSCGNCHYCREGHPTRCVELTVIGARNPGAYAEYTKLPEGHVKILPDSIDDDTAALVEPSAVVAHGFYRASITP
GSSVAIMGVGSIGLLAVQWAKIFGASKVIAIDIDDHKLKIAQELGADIVVNPMKENAEGIVKQHTDELGVDLAVESAGSP
ITSEQVLALPKKGGEVVFLGIPYGDITLKRYYFEKIVRNELRILGSWNALSAPFPGKEWNATLHYMSTGQLNIKPMISHR
LGLQAGPEIFHQITNKLSDAVKVIFHPGK
>Mature_349_residues
MEALNLYGIEDLRYEDTPKPMIEKDDDVIIKVKSVGICGSDTSRYKKLGPYVEGMTFGHEFAGEVIEVGKSVTNFKSGDR
VVACPTFSCGNCHYCREGHPTRCVELTVIGARNPGAYAEYTKLPEGHVKILPDSIDDDTAALVEPSAVVAHGFYRASITP
GSSVAIMGVGSIGLLAVQWAKIFGASKVIAIDIDDHKLKIAQELGADIVVNPMKENAEGIVKQHTDELGVDLAVESAGSP
ITSEQVLALPKKGGEVVFLGIPYGDITLKRYYFEKIVRNELRILGSWNALSAPFPGKEWNATLHYMSTGQLNIKPMISHR
LGLQAGPEIFHQITNKLSDAVKVIFHPGK

Specific function: Converts galactitol 1-phosphate to tagatose 6-phosphate [H]

COG id: COG1063

COG function: function code ER; Threonine dehydrogenase and related Zn-dependent dehydrogenases

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the zinc-containing alcohol dehydrogenase family [H]

Homologues:

Organism=Homo sapiens, GI156627571, Length=344, Percent_Identity=28.1976744186047, Blast_Score=138, Evalue=7e-33,
Organism=Homo sapiens, GI4501929, Length=271, Percent_Identity=30.2583025830258, Blast_Score=102, Evalue=7e-22,
Organism=Homo sapiens, GI156523966, Length=339, Percent_Identity=26.8436578171091, Blast_Score=100, Evalue=2e-21,
Organism=Homo sapiens, GI4501939, Length=342, Percent_Identity=26.9005847953216, Blast_Score=100, Evalue=2e-21,
Organism=Homo sapiens, GI34577061, Length=270, Percent_Identity=30.3703703703704, Blast_Score=97, Evalue=2e-20,
Organism=Homo sapiens, GI4501933, Length=273, Percent_Identity=30.03663003663, Blast_Score=96, Evalue=4e-20,
Organism=Homo sapiens, GI71565152, Length=367, Percent_Identity=25.3405994550409, Blast_Score=92, Evalue=7e-19,
Organism=Homo sapiens, GI71565154, Length=258, Percent_Identity=27.1317829457364, Blast_Score=82, Evalue=1e-15,
Organism=Homo sapiens, GI71743840, Length=211, Percent_Identity=26.0663507109005, Blast_Score=77, Evalue=2e-14,
Organism=Homo sapiens, GI262073058, Length=211, Percent_Identity=26.0663507109005, Blast_Score=77, Evalue=2e-14,
Organism=Homo sapiens, GI194239674, Length=272, Percent_Identity=27.5735294117647, Blast_Score=67, Evalue=3e-11,
Organism=Homo sapiens, GI13236495, Length=272, Percent_Identity=27.5735294117647, Blast_Score=67, Evalue=3e-11,
Organism=Escherichia coli, GI1788407, Length=309, Percent_Identity=33.9805825242718, Blast_Score=193, Evalue=1e-50,
Organism=Escherichia coli, GI1788073, Length=329, Percent_Identity=34.0425531914894, Blast_Score=190, Evalue=1e-49,
Organism=Escherichia coli, GI1790045, Length=334, Percent_Identity=30.8383233532934, Blast_Score=158, Evalue=5e-40,
Organism=Escherichia coli, GI1788075, Length=363, Percent_Identity=29.4765840220386, Blast_Score=149, Evalue=4e-37,
Organism=Escherichia coli, GI226510992, Length=304, Percent_Identity=31.9078947368421, Blast_Score=140, Evalue=1e-34,
Organism=Escherichia coli, GI1786825, Length=279, Percent_Identity=33.3333333333333, Blast_Score=139, Evalue=4e-34,
Organism=Escherichia coli, GI1787863, Length=303, Percent_Identity=29.042904290429, Blast_Score=125, Evalue=3e-30,
Organism=Escherichia coli, GI87082125, Length=353, Percent_Identity=27.1954674220963, Blast_Score=122, Evalue=3e-29,
Organism=Escherichia coli, GI1790718, Length=304, Percent_Identity=25.9868421052632, Blast_Score=119, Evalue=4e-28,
Organism=Escherichia coli, GI87081918, Length=267, Percent_Identity=33.3333333333333, Blast_Score=108, Evalue=4e-25,
Organism=Escherichia coli, GI1786552, Length=330, Percent_Identity=25.7575757575758, Blast_Score=80, Evalue=2e-16,
Organism=Escherichia coli, GI1787572, Length=292, Percent_Identity=25, Blast_Score=62, Evalue=8e-11,
Organism=Caenorhabditis elegans, GI17562876, Length=350, Percent_Identity=30.2857142857143, Blast_Score=151, Evalue=5e-37,
Organism=Caenorhabditis elegans, GI17562878, Length=364, Percent_Identity=29.3956043956044, Blast_Score=132, Evalue=3e-31,
Organism=Caenorhabditis elegans, GI71988145, Length=219, Percent_Identity=33.3333333333333, Blast_Score=91, Evalue=9e-19,
Organism=Caenorhabditis elegans, GI17562582, Length=261, Percent_Identity=29.1187739463602, Blast_Score=75, Evalue=5e-14,
Organism=Caenorhabditis elegans, GI17562584, Length=288, Percent_Identity=27.7777777777778, Blast_Score=75, Evalue=7e-14,
Organism=Saccharomyces cerevisiae, GI6322619, Length=358, Percent_Identity=27.6536312849162, Blast_Score=134, Evalue=2e-32,
Organism=Saccharomyces cerevisiae, GI6319955, Length=358, Percent_Identity=27.6536312849162, Blast_Score=134, Evalue=3e-32,
Organism=Saccharomyces cerevisiae, GI6323099, Length=335, Percent_Identity=28.955223880597, Blast_Score=131, Evalue=2e-31,
Organism=Saccharomyces cerevisiae, GI6319258, Length=289, Percent_Identity=32.5259515570934, Blast_Score=122, Evalue=1e-28,
Organism=Saccharomyces cerevisiae, GI6319257, Length=384, Percent_Identity=25.78125, Blast_Score=108, Evalue=1e-24,
Organism=Saccharomyces cerevisiae, GI6320033, Length=344, Percent_Identity=25, Blast_Score=95, Evalue=2e-20,
Organism=Saccharomyces cerevisiae, GI6319621, Length=328, Percent_Identity=26.5243902439024, Blast_Score=89, Evalue=1e-18,
Organism=Saccharomyces cerevisiae, GI6323729, Length=285, Percent_Identity=26.3157894736842, Blast_Score=87, Evalue=5e-18,
Organism=Saccharomyces cerevisiae, GI6324486, Length=282, Percent_Identity=27.3049645390071, Blast_Score=84, Evalue=4e-17,
Organism=Saccharomyces cerevisiae, GI6319949, Length=308, Percent_Identity=28.5714285714286, Blast_Score=81, Evalue=3e-16,
Organism=Saccharomyces cerevisiae, GI6323961, Length=329, Percent_Identity=25.531914893617, Blast_Score=80, Evalue=5e-16,
Organism=Saccharomyces cerevisiae, GI6319520, Length=258, Percent_Identity=27.906976744186, Blast_Score=79, Evalue=1e-15,
Organism=Saccharomyces cerevisiae, GI6323980, Length=287, Percent_Identity=28.9198606271777, Blast_Score=72, Evalue=2e-13,
Organism=Drosophila melanogaster, GI17737897, Length=351, Percent_Identity=32.1937321937322, Blast_Score=148, Evalue=5e-36,
Organism=Drosophila melanogaster, GI17137530, Length=350, Percent_Identity=31.7142857142857, Blast_Score=147, Evalue=1e-35,
Organism=Drosophila melanogaster, GI17737895, Length=300, Percent_Identity=26.6666666666667, Blast_Score=91, Evalue=1e-18,
Organism=Drosophila melanogaster, GI45550770, Length=171, Percent_Identity=33.3333333333333, Blast_Score=87, Evalue=1e-17,
Organism=Drosophila melanogaster, GI45551930, Length=171, Percent_Identity=33.3333333333333, Blast_Score=87, Evalue=1e-17,
Organism=Drosophila melanogaster, GI221457811, Length=171, Percent_Identity=33.3333333333333, Blast_Score=87, Evalue=1e-17,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR013149
- InterPro:   IPR013154
- InterPro:   IPR002085
- InterPro:   IPR002328
- InterPro:   IPR011032
- InterPro:   IPR016040 [H]

Pfam domain/function: PF08240 ADH_N; PF00107 ADH_zinc_N [H]

EC number: =1.1.1.251 [H]

Molecular weight: Translated: 37908; Mature: 37908

Theoretical pI: Translated: 6.29; Mature: 6.29

Prosite motif: PS00059 ADH_ZINC

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.7 %Cys     (Translated Protein)
2.0 %Met     (Translated Protein)
3.7 %Cys+Met (Translated Protein)
1.7 %Cys     (Mature Protein)
2.0 %Met     (Mature Protein)
3.7 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MEALNLYGIEDLRYEDTPKPMIEKDDDVIIKVKSVGICGSDTSRYKKLGPYVEGMTFGHE
CCCCEECCCCCCCCCCCCCCCCCCCCCEEEEEEEEEEECCCHHHHHHHCCHHCCEECCHH
FAGEVIEVGKSVTNFKSGDRVVACPTFSCGNCHYCREGHPTRCVELTVIGARNPGAYAEY
HHHHHHHHCCHHHCCCCCCEEEEECCCCCCCCCCCCCCCCCEEEEEEEEECCCCCCCHHH
TKLPEGHVKILPDSIDDDTAALVEPSAVVAHGFYRASITPGSSVAIMGVGSIGLLAVQWA
HCCCCCCEEEECCCCCCCCHHHCCCCHHEEECEEEEEECCCCCEEEEEECCHHHHHHHHH
KIFGASKVIAIDIDDHKLKIAQELGADIVVNPMKENAEGIVKQHTDELGVDLAVESAGSP
HHHCCCEEEEEEECCCHHHHHHHCCCCEEEECCCCCHHHHHHHHHHHCCCEEEEECCCCC
ITSEQVLALPKKGGEVVFLGIPYGDITLKRYYFEKIVRNELRILGSWNALSAPFPGKEWN
CCCCCEEEECCCCCEEEEEECCCCCEEHHHHHHHHHHHHHHEEEECCCCCCCCCCCCCCC
ATLHYMSTGQLNIKPMISHRLGLQAGPEIFHQITNKLSDAVKVIFHPGK
EEEEEEECCCEEEEEHHHHHCCCCCCHHHHHHHHHHHHHEEEEEECCCC
>Mature Secondary Structure
MEALNLYGIEDLRYEDTPKPMIEKDDDVIIKVKSVGICGSDTSRYKKLGPYVEGMTFGHE
CCCCEECCCCCCCCCCCCCCCCCCCCCEEEEEEEEEEECCCHHHHHHHCCHHCCEECCHH
FAGEVIEVGKSVTNFKSGDRVVACPTFSCGNCHYCREGHPTRCVELTVIGARNPGAYAEY
HHHHHHHHCCHHHCCCCCCEEEEECCCCCCCCCCCCCCCCCEEEEEEEEECCCCCCCHHH
TKLPEGHVKILPDSIDDDTAALVEPSAVVAHGFYRASITPGSSVAIMGVGSIGLLAVQWA
HCCCCCCEEEECCCCCCCCHHHCCCCHHEEECEEEEEECCCCCEEEEEECCHHHHHHHHH
KIFGASKVIAIDIDDHKLKIAQELGADIVVNPMKENAEGIVKQHTDELGVDLAVESAGSP
HHHCCCEEEEEEECCCHHHHHHHCCCCEEEECCCCCHHHHHHHHHHHCCCEEEEECCCCC
ITSEQVLALPKKGGEVVFLGIPYGDITLKRYYFEKIVRNELRILGSWNALSAPFPGKEWN
CCCCCEEEECCCCCEEEEEECCCCCEEHHHHHHHHHHHHHHEEEECCCCCCCCCCCCCCC
ATLHYMSTGQLNIKPMISHRLGLQAGPEIFHQITNKLSDAVKVIFHPGK
EEEEEEECCCEEEEEHHHHHCCCCCCHHHHHHHHHHHHHEEEEEECCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 11206551; 11258796 [H]