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The map label for this gene is kdgA [H]

Identifier: 23100158

GI number: 23100158

Start: 2773342

End: 2773974

Strand: Reverse

Name: kdgA [H]

Synonym: OB2703

Alternate gene names: 23100158

Gene position: 2773974-2773342 (Counterclockwise)

Preceding gene: 23100159

Following gene: 23100157

Centisome position: 76.41

GC content: 36.49

Gene sequence:

>633_bases
ATGTCAGTAATAGAAGGTATTCAAGAAGAAAAAGTAGTAGCAGTTATCCGAAAAGCAAATTCGGATAATATTGTACCTAT
TTTGGAAGCGTTAGCAAAAGGTGGGATTCATCATGTTGAAATAACAGCTGAAACCCCAAATGTAGAAAATGTAATTAAAA
CAGCAGTTCAAAACGTAAATGAAAAAATTCAAGTTGGTGCTGGTACAGTACTAGATCCAGAGACTGCGAGAACATTAATT
TTAGCTGGAGCAACGTTTATCGTATCTCCAACATTAAATATAAAAACAGTAGAAATGTGTCAACGGTATGGTGTAGAACA
TATTCCTGGTGTATTTACACCGACTGAAGTATTAACTGCCTATGAGCATGGTGCGAGAATGGTGAAAATTTTTCCTATCG
ATGCAGTCGGTCCAAACTATGTGAAAAATATTCGTGGGCCATTACCACATGTGAAAGCGATGGTAACTGGTGGAATTACC
TTGGATAATATCGGTGATTATTTGAAAGCAGGAAGTTTTGCTGTTGGAACAGGAAGTAGCTTAGTAAATGCTCCGTTACT
GCAAACAAAAGAAGATTATGATGAACTAACAGAACGTGCGAAACAATATATTCAAAGCGCAAAAGAAGTTTAG

Upstream 100 bases:

>100_bases
GATGCCATCGTGGTGTATTTATTAGATACGTATCCAGCTAACCGTACTGTGGAAAGTCTAAACCAAAAACATACTAATTT
AGAATAAGGTGGGATTATTT

Downstream 100 bases:

>100_bases
AAAAACAATTGCTAAAACACCAAGAGGGAGGAACATTTTATGAGAAAAAATAAAATCGCTTTCATAATGTTGGTTATCAG
TTTAATAGCTTTTGTACTTT

Product: 2-keto-3-deoxy-6-phospho-gluconate aldolase

Products: NA

Alternate protein names: 4-hydroxy-2-oxoglutarate aldolase; 2-keto-4-hydroxyglutarate aldolase; KHG-aldolase; 2-dehydro-3-deoxy-phosphogluconate aldolase; 2-keto-3-deoxy-6-phosphogluconate aldolase; KDPG-aldolase; Phospho-2-dehydro-3-deoxygluconate aldolase; Phospho-2-keto-3-deoxygluconate aldolase [H]

Number of amino acids: Translated: 210; Mature: 209

Protein sequence:

>210_residues
MSVIEGIQEEKVVAVIRKANSDNIVPILEALAKGGIHHVEITAETPNVENVIKTAVQNVNEKIQVGAGTVLDPETARTLI
LAGATFIVSPTLNIKTVEMCQRYGVEHIPGVFTPTEVLTAYEHGARMVKIFPIDAVGPNYVKNIRGPLPHVKAMVTGGIT
LDNIGDYLKAGSFAVGTGSSLVNAPLLQTKEDYDELTERAKQYIQSAKEV

Sequences:

>Translated_210_residues
MSVIEGIQEEKVVAVIRKANSDNIVPILEALAKGGIHHVEITAETPNVENVIKTAVQNVNEKIQVGAGTVLDPETARTLI
LAGATFIVSPTLNIKTVEMCQRYGVEHIPGVFTPTEVLTAYEHGARMVKIFPIDAVGPNYVKNIRGPLPHVKAMVTGGIT
LDNIGDYLKAGSFAVGTGSSLVNAPLLQTKEDYDELTERAKQYIQSAKEV
>Mature_209_residues
SVIEGIQEEKVVAVIRKANSDNIVPILEALAKGGIHHVEITAETPNVENVIKTAVQNVNEKIQVGAGTVLDPETARTLIL
AGATFIVSPTLNIKTVEMCQRYGVEHIPGVFTPTEVLTAYEHGARMVKIFPIDAVGPNYVKNIRGPLPHVKAMVTGGITL
DNIGDYLKAGSFAVGTGSSLVNAPLLQTKEDYDELTERAKQYIQSAKEV

Specific function: KEY ENZYME IN THE ENTNER-DOUDOROFF PATHWAY. PARTICIPATES IN THE REGULATION OF THE INTRACELLULAR LEVEL OF GLYOXYLATE. [C]

COG id: COG0800

COG function: function code G; 2-keto-3-deoxy-6-phosphogluconate aldolase

Gene ontology:

Cell location: Cytoplasm [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the KHG/KDPG aldolase family [H]

Homologues:

Organism=Escherichia coli, GI48994954, Length=199, Percent_Identity=31.1557788944724, Blast_Score=110, Evalue=6e-26,
Organism=Escherichia coli, GI1788156, Length=188, Percent_Identity=34.5744680851064, Blast_Score=100, Evalue=1e-22,

Paralogues:

None

Copy number: 2,000 Molecules/Cell In: Glucose minimal media [C]

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR000887
- InterPro:   IPR013785 [H]

Pfam domain/function: PF01081 Aldolase [H]

EC number: =4.1.3.16; =4.1.2.14 [H]

Molecular weight: Translated: 22585; Mature: 22454

Theoretical pI: Translated: 5.64; Mature: 5.64

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.5 %Cys     (Translated Protein)
1.9 %Met     (Translated Protein)
2.4 %Cys+Met (Translated Protein)
0.5 %Cys     (Mature Protein)
1.4 %Met     (Mature Protein)
1.9 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MSVIEGIQEEKVVAVIRKANSDNIVPILEALAKGGIHHVEITAETPNVENVIKTAVQNVN
CCHHCCHHHHHHHHHHHHCCCCCHHHHHHHHHHCCCEEEEEEECCCCHHHHHHHHHHHHC
EKIQVGAGTVLDPETARTLILAGATFIVSPTLNIKTVEMCQRYGVEHIPGVFTPTEVLTA
HHEEECCCCEECCHHHHEEEEECCEEEECCCCCHHHHHHHHHCCHHHCCCCCCHHHHHHH
YEHGARMVKIFPIDAVGPNYVKNIRGPLPHVKAMVTGGITLDNIGDYLKAGSFAVGTGSS
HHCCCEEEEEEEECCCCHHHHHHHCCCCCHHHHHHCCCCCHHHHHHHHHCCCEEECCCCH
LVNAPLLQTKEDYDELTERAKQYIQSAKEV
HHCCCCCCCHHHHHHHHHHHHHHHHHHHCC
>Mature Secondary Structure 
SVIEGIQEEKVVAVIRKANSDNIVPILEALAKGGIHHVEITAETPNVENVIKTAVQNVN
CHHCCHHHHHHHHHHHHCCCCCHHHHHHHHHHCCCEEEEEEECCCCHHHHHHHHHHHHC
EKIQVGAGTVLDPETARTLILAGATFIVSPTLNIKTVEMCQRYGVEHIPGVFTPTEVLTA
HHEEECCCCEECCHHHHEEEEECCEEEECCCCCHHHHHHHHHCCHHHCCCCCCHHHHHHH
YEHGARMVKIFPIDAVGPNYVKNIRGPLPHVKAMVTGGITLDNIGDYLKAGSFAVGTGSS
HHCCCEEEEEEEECCCCHHHHHHHCCCCCHHHHHHCCCCCHHHHHHHHHCCCEEECCCCH
LVNAPLLQTKEDYDELTERAKQYIQSAKEV
HHCCCCCCCHHHHHHHHHHHHHHHHHHHCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 8760912; 9384377 [H]