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The map label for this gene is purU [H]

Identifier: 23100148

GI number: 23100148

Start: 2764965

End: 2765867

Strand: Reverse

Name: purU [H]

Synonym: OB2693

Alternate gene names: 23100148

Gene position: 2765867-2764965 (Counterclockwise)

Preceding gene: 23100150

Following gene: 23100142

Centisome position: 76.18

GC content: 32.89

Gene sequence:

>903_bases
ATGTACCGATATTCAGAAAATAAATTAGATGTATTTCAAGAAAGGTATAAAGATAGAGCTCGACTATTGATAAAATGTCC
AGACAAACAAGGAATTGTCGCTGCAGTTTCAACATTTTTACATGAACAAGGAGCTAATATTGTAGCTTCAAATCAATATA
CTGTTGATCCAGAGGGTGGACAATTTTTTATGCGCTTAGAATTTGAAATCAAAGGTTTATCTGAACAAGTAGGGCAGTTA
AAGGAAAAGTTTCAAATTGTAGCTGAACATATGGATATGGAATGGCGAATTGCTATGATGAGCGAGCTTAAAAATGTAGC
TATCTTTGTTTCGAAAGAACCTCATTGCTTACTTGAATTGTTATGGGAATGGCAAAGTGGAGATTTATTAGCAAATATTA
AAGTAGTAATTAGTAATCATGAAACTGCAAGGGAAATGGTTGAAGCTGTTGGAATTCCTTTTTATCATATTCCAGTTACG
AAAGAACAAAAAAAAGAAGCAGAGGAAAAACAAAATCAAATCCTCAAAAAATATGATATCGAGCTGATTATATTAGCAAG
ATATATGCAAATATTGAGCCCTCATTTTGTAGAAAAATATGAGAGTAAAATTATAAATATTCATCATTCATTTTTACCTG
CTTTTATTGGAGCAAAACCGTATGAACGTGCATATGATCGAGGGGTCAAAATGATTGGTGCAACTTCACATTATGTAACA
AATGATTTAGATGAAGGGCCGATTATTGAACAAGATATTGATCGTGTCAATCATGAACAAGATGCAGCAGATTTAAAGAA
AATCGGGCAATCAATTGAGAGACGTGTTCTTGCACGAGCTGTAAAATGGCATTTAGAAGATCGTATTCTTGTGCATGGAA
ACAAGACGATTATTTTTGAATAA

Upstream 100 bases:

>100_bases
CTTTCGTACTTTAAAGTACTATAAAGTAAAACTATTTCAGTGTGATATTCATATTCGTTACATGCGGGTAAATAAAAGAC
GAGAGAAAGTTGTGATTTAT

Downstream 100 bases:

>100_bases
AGTAAAAGAACGAGCTTTAGGATGGAATCTTATGTTCATCCAGAGCTCGTTCTTAATATAATTGATGTAAAATCCGTATG
CGTTGTTTGATTTTCATTGC

Product: formyltetrahydrofolate deformylase

Products: NA

Alternate protein names: Formyl-FH(4) hydrolase [H]

Number of amino acids: Translated: 300; Mature: 300

Protein sequence:

>300_residues
MYRYSENKLDVFQERYKDRARLLIKCPDKQGIVAAVSTFLHEQGANIVASNQYTVDPEGGQFFMRLEFEIKGLSEQVGQL
KEKFQIVAEHMDMEWRIAMMSELKNVAIFVSKEPHCLLELLWEWQSGDLLANIKVVISNHETAREMVEAVGIPFYHIPVT
KEQKKEAEEKQNQILKKYDIELIILARYMQILSPHFVEKYESKIINIHHSFLPAFIGAKPYERAYDRGVKMIGATSHYVT
NDLDEGPIIEQDIDRVNHEQDAADLKKIGQSIERRVLARAVKWHLEDRILVHGNKTIIFE

Sequences:

>Translated_300_residues
MYRYSENKLDVFQERYKDRARLLIKCPDKQGIVAAVSTFLHEQGANIVASNQYTVDPEGGQFFMRLEFEIKGLSEQVGQL
KEKFQIVAEHMDMEWRIAMMSELKNVAIFVSKEPHCLLELLWEWQSGDLLANIKVVISNHETAREMVEAVGIPFYHIPVT
KEQKKEAEEKQNQILKKYDIELIILARYMQILSPHFVEKYESKIINIHHSFLPAFIGAKPYERAYDRGVKMIGATSHYVT
NDLDEGPIIEQDIDRVNHEQDAADLKKIGQSIERRVLARAVKWHLEDRILVHGNKTIIFE
>Mature_300_residues
MYRYSENKLDVFQERYKDRARLLIKCPDKQGIVAAVSTFLHEQGANIVASNQYTVDPEGGQFFMRLEFEIKGLSEQVGQL
KEKFQIVAEHMDMEWRIAMMSELKNVAIFVSKEPHCLLELLWEWQSGDLLANIKVVISNHETAREMVEAVGIPFYHIPVT
KEQKKEAEEKQNQILKKYDIELIILARYMQILSPHFVEKYESKIINIHHSFLPAFIGAKPYERAYDRGVKMIGATSHYVT
NDLDEGPIIEQDIDRVNHEQDAADLKKIGQSIERRVLARAVKWHLEDRILVHGNKTIIFE

Specific function: Produces Formate From Formyl-Tetrahydrofolate. Provides The Major Source Of Formate For The Purt-Dependent Synthesis Of 5'-Phosphoribosyl-N-Formylglycinamide (Fgar) During Aerobic Growth. Has A Role In Regulating The One-Carbon Pool. [C]

COG id: COG0788

COG function: function code F; Formyltetrahydrofolate hydrolase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 ACT domain [H]

Homologues:

Organism=Homo sapiens, GI4503915, Length=180, Percent_Identity=31.1111111111111, Blast_Score=88, Evalue=9e-18,
Organism=Homo sapiens, GI209869995, Length=180, Percent_Identity=31.1111111111111, Blast_Score=88, Evalue=9e-18,
Organism=Homo sapiens, GI209869993, Length=180, Percent_Identity=31.1111111111111, Blast_Score=88, Evalue=9e-18,
Organism=Escherichia coli, GI1787483, Length=274, Percent_Identity=41.970802919708, Blast_Score=225, Evalue=3e-60,
Organism=Escherichia coli, GI1788846, Length=193, Percent_Identity=25.9067357512953, Blast_Score=80, Evalue=1e-16,
Organism=Caenorhabditis elegans, GI17567511, Length=148, Percent_Identity=37.1621621621622, Blast_Score=100, Evalue=7e-22,
Organism=Drosophila melanogaster, GI24582400, Length=172, Percent_Identity=30.2325581395349, Blast_Score=66, Evalue=2e-11,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR002912
- InterPro:   IPR002376
- InterPro:   IPR004810 [H]

Pfam domain/function: PF01842 ACT; PF00551 Formyl_trans_N [H]

EC number: =3.5.1.10 [H]

Molecular weight: Translated: 34895; Mature: 34895

Theoretical pI: Translated: 6.38; Mature: 6.38

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.7 %Cys     (Translated Protein)
3.0 %Met     (Translated Protein)
3.7 %Cys+Met (Translated Protein)
0.7 %Cys     (Mature Protein)
3.0 %Met     (Mature Protein)
3.7 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MYRYSENKLDVFQERYKDRARLLIKCPDKQGIVAAVSTFLHEQGANIVASNQYTVDPEGG
CCCCCCCHHHHHHHHHHHHCEEEEECCCCCCHHHHHHHHHHHCCCCEEECCEEEECCCCC
QFFMRLEFEIKGLSEQVGQLKEKFQIVAEHMDMEWRIAMMSELKNVAIFVSKEPHCLLEL
EEEEEEEEEECCHHHHHHHHHHHHHHHHHHCCCHHHHHHHHHHCCEEEEEECCCHHHHHH
LWEWQSGDLLANIKVVISNHETAREMVEAVGIPFYHIPVTKEQKKEAEEKQNQILKKYDI
HHHCCCCCEEEEEEEEEECCHHHHHHHHHHCCCEEEECCCHHHHHHHHHHHHHHHHHHCC
ELIILARYMQILSPHFVEKYESKIINIHHSFLPAFIGAKPYERAYDRGVKMIGATSHYVT
HHHHHHHHHHHHCHHHHHHHHHHHHHHHHHHHHHHHCCCCHHHHHHCCCEEEECCHHHHC
NDLDEGPIIEQDIDRVNHEQDAADLKKIGQSIERRVLARAVKWHLEDRILVHGNKTIIFE
CCCCCCCCCHHHHHHCCCCCHHHHHHHHHHHHHHHHHHHHHHHHCCCEEEEECCEEEEEC
>Mature Secondary Structure
MYRYSENKLDVFQERYKDRARLLIKCPDKQGIVAAVSTFLHEQGANIVASNQYTVDPEGG
CCCCCCCHHHHHHHHHHHHCEEEEECCCCCCHHHHHHHHHHHCCCCEEECCEEEECCCCC
QFFMRLEFEIKGLSEQVGQLKEKFQIVAEHMDMEWRIAMMSELKNVAIFVSKEPHCLLEL
EEEEEEEEEECCHHHHHHHHHHHHHHHHHHCCCHHHHHHHHHHCCEEEEEECCCHHHHHH
LWEWQSGDLLANIKVVISNHETAREMVEAVGIPFYHIPVTKEQKKEAEEKQNQILKKYDI
HHHCCCCCEEEEEEEEEECCHHHHHHHHHHCCCEEEECCCHHHHHHHHHHHHHHHHHHCC
ELIILARYMQILSPHFVEKYESKIINIHHSFLPAFIGAKPYERAYDRGVKMIGATSHYVT
HHHHHHHHHHHHCHHHHHHHHHHHHHHHHHHHHHHHCCCCHHHHHHCCCEEEECCHHHHC
NDLDEGPIIEQDIDRVNHEQDAADLKKIGQSIERRVLARAVKWHLEDRILVHGNKTIIFE
CCCCCCCCCHHHHHHCCCCCHHHHHHHHHHHHHHHHHHHHHHHHCCCEEEEECCEEEEEC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 9384377 [H]