Click here to switch to the map view.

The map label for this gene is pdxT [H]

Identifier: 23100141

GI number: 23100141

Start: 2756354

End: 2756929

Strand: Reverse

Name: pdxT [H]

Synonym: OB2686

Alternate gene names: 23100141

Gene position: 2756929-2756354 (Counterclockwise)

Preceding gene: 23100142

Following gene: 23100140

Centisome position: 75.94

GC content: 35.94

Gene sequence:

>576_bases
TTGGGCTTACAAGGGGCCATAAGTGAACATGTAAACCGGTTAAAGGAATTAGAACAGCAACCTGTAGTTGTCAAAAAATC
AACGGATTTACATCAACTAGATGGTTTAATTATTCCTGGTGGTGAGAGTACAGCTATTTGGAAACTGATTGAAGAGAATA
AATTATATGAACCTATCCAGAACTTTGCAAATGAAGGAAAAGCTATTTTTGGAACTTGCGCAGGGTTAGTACTATTAAGT
AAAACAACAATAGGAAGGGATTATACTCCTACCTTAAAACTTATGGATATAACCGTTCAACGTAATGGATTTGGAAGACA
GAAGGATAGTTTTGAAGCTACTATTCAAGCTAAATATATGGATGATAGCTATCATGCTGTATTTATCCGTGCGCCTTATA
TCGAATCCGTTGGAGAAGGTGTGGAAGTAATAGCTTCCTATAATGATAAAATCGTAGCTGCACGTCAAAAAAACGTTCTT
GTTTGTGCATTTCATCCCGAACTGACGGATGATGATCGGTTTTTGGAGATGTTTTTAACTATGATTTCTTCAAACAAGAA
CTTCAATCATGTTTAG

Upstream 100 bases:

>100_bases
ATGTGCATTCTCTAGCTGCTGATCAACGTATGCAAGAACGTGGTTGGTAATAACGTAAAGGGTGGTGAATAGCATGTGCA
ATTCTTTTCGTATAGGAGTA

Downstream 100 bases:

>100_bases
TCAATATCATTAAGGGAAAACACATACTAACAAACATGATAGGAGGTTTTCATCATGAGTGATGGTATGAAAGATAAAGC
GAAAGCAATTGGAAAAAAAA

Product: amidotransferase

Products: NA

Alternate protein names: Glutamine amidotransferase glutaminase subunit pdxT [H]

Number of amino acids: Translated: 191; Mature: 190

Protein sequence:

>191_residues
MGLQGAISEHVNRLKELEQQPVVVKKSTDLHQLDGLIIPGGESTAIWKLIEENKLYEPIQNFANEGKAIFGTCAGLVLLS
KTTIGRDYTPTLKLMDITVQRNGFGRQKDSFEATIQAKYMDDSYHAVFIRAPYIESVGEGVEVIASYNDKIVAARQKNVL
VCAFHPELTDDDRFLEMFLTMISSNKNFNHV

Sequences:

>Translated_191_residues
MGLQGAISEHVNRLKELEQQPVVVKKSTDLHQLDGLIIPGGESTAIWKLIEENKLYEPIQNFANEGKAIFGTCAGLVLLS
KTTIGRDYTPTLKLMDITVQRNGFGRQKDSFEATIQAKYMDDSYHAVFIRAPYIESVGEGVEVIASYNDKIVAARQKNVL
VCAFHPELTDDDRFLEMFLTMISSNKNFNHV
>Mature_190_residues
GLQGAISEHVNRLKELEQQPVVVKKSTDLHQLDGLIIPGGESTAIWKLIEENKLYEPIQNFANEGKAIFGTCAGLVLLSK
TTIGRDYTPTLKLMDITVQRNGFGRQKDSFEATIQAKYMDDSYHAVFIRAPYIESVGEGVEVIASYNDKIVAARQKNVLV
CAFHPELTDDDRFLEMFLTMISSNKNFNHV

Specific function: Involved in the hydrolysis of glutamine to glutamate and ammonia. Channels an ammonia molecule to pdxS [H]

COG id: COG0311

COG function: function code H; Predicted glutamine amidotransferase involved in pyridoxine biosynthesis

Gene ontology:

Cell location: Cytoplasmic

Metaboloic importance: NA

Operon status: Not Known

Operon components: None

Similarity: Belongs to the glutamine amidotransferase pdxT/SNO family [H]

Homologues:

Organism=Saccharomyces cerevisiae, GI6323742, Length=208, Percent_Identity=38.4615384615385, Blast_Score=119, Evalue=4e-28,
Organism=Saccharomyces cerevisiae, GI6323995, Length=207, Percent_Identity=36.7149758454106, Blast_Score=106, Evalue=3e-24,
Organism=Saccharomyces cerevisiae, GI6321048, Length=207, Percent_Identity=37.1980676328502, Blast_Score=106, Evalue=3e-24,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR002161
- InterPro:   IPR021196 [H]

Pfam domain/function: PF01174 SNO [H]

EC number: NA

Molecular weight: Translated: 21424; Mature: 21293

Theoretical pI: Translated: 5.63; Mature: 5.63

Prosite motif: PS01236 PDXT_SNO_1 ; PS51130 PDXT_SNO_2

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.0 %Cys     (Translated Protein)
2.6 %Met     (Translated Protein)
3.7 %Cys+Met (Translated Protein)
1.1 %Cys     (Mature Protein)
2.1 %Met     (Mature Protein)
3.2 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MGLQGAISEHVNRLKELEQQPVVVKKSTDLHQLDGLIIPGGESTAIWKLIEENKLYEPIQ
CCCCHHHHHHHHHHHHHHHCCEEEECCCCHHHHCCEEECCCCCHHHHHHHHHCCHHHHHH
NFANEGKAIFGTCAGLVLLSKTTIGRDYTPTLKLMDITVQRNGFGRQKDSFEATIQAKYM
HHHHCCCEEEHHHHHHHHHHHHCCCCCCCCCEEEEEEEEECCCCCCCCCCCEEEEEEEEE
DDSYHAVFIRAPYIESVGEGVEVIASYNDKIVAARQKNVLVCAFHPELTDDDRFLEMFLT
CCCEEEEEEECCCHHHHCCCCEEEECCCCEEEEEECCCEEEEEECCCCCCCHHHHHHHHH
MISSNKNFNHV
HHHCCCCCCCC
>Mature Secondary Structure 
GLQGAISEHVNRLKELEQQPVVVKKSTDLHQLDGLIIPGGESTAIWKLIEENKLYEPIQ
CCCHHHHHHHHHHHHHHHCCEEEECCCCHHHHCCEEECCCCCHHHHHHHHHCCHHHHHH
NFANEGKAIFGTCAGLVLLSKTTIGRDYTPTLKLMDITVQRNGFGRQKDSFEATIQAKYM
HHHHCCCEEEHHHHHHHHHHHHCCCCCCCCCEEEEEEEEECCCCCCCCCCCEEEEEEEEE
DDSYHAVFIRAPYIESVGEGVEVIASYNDKIVAARQKNVLVCAFHPELTDDDRFLEMFLT
CCCEEEEEEECCCHHHHCCCCEEEECCCCEEEEEECCCEEEEEECCCCCCCHHHHHHHHH
MISSNKNFNHV
HHHCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 12235376 [H]