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The map label for this gene is rffA [H]

Identifier: 23099871

GI number: 23099871

Start: 2471232

End: 2472362

Strand: Reverse

Name: rffA [H]

Synonym: OB2416

Alternate gene names: 23099871

Gene position: 2472362-2471232 (Counterclockwise)

Preceding gene: 23099872

Following gene: 23099870

Centisome position: 68.1

GC content: 36.6

Gene sequence:

>1131_bases
TTGATACCATTTAATATACCTTGTCATTTAGGTCAAGAAGAAGAAGCAATCGCTGAAGCAATTAAAGAGCAAAAGTTATC
TGGGAATGGCCGTTTTAGTCGATATTGTACGACATGGTTGGAGAATCAGTTAGACGCAAAAAAAGCAATATTAACTCCGT
CCTGTACGGCCGCTTTGGAAATGTCTGCCATTTTGACAGATGTGTCAGAAGGAGACGAAGTTATTATGCCATCCTATACA
TTTGTGTCTACCGCTAATGCATTTGCATTGCGAGGAGCACATATTCGGTTTGTAGATGTGGAACCAACGACAATGAATAT
TGATCCGAAGGCTATTGAAGCAGCAATTAATCATCGAACTAAAGCAATCGTAGTGGTTCACTATGGGGGTGTGGCTTGTG
ATATGATTCCTATCATGAACTTGGCAAACGAACATCAATTATGGGTAATTGAAGATGCGGCACAAGGATTAAAGAGTCAG
TATAATGGACGTCCATTAGGAACTATTGGTCATCTTGGGACATTTAGTTTTCATGAAACAAAAAATTATACATGTGGAGA
AGGCGGAGCTCTTATAATAAATGACGATGCTTTTATCGAACGTGCAGAGATCATACAGGAAAAAGGCACAGACCGTTCTA
AATTTGTTCGCGGGGAAGTAGACAAATATACATGGCGAGATATCGGATCTTCTTATTTAACGAGTGAATTAAATGCAGCC
TATTTATCTGTTCAATTGGATCAAGCAGAAGTCATTAACCAAGATCGTCTTGCTACTTGGTATTTGTATCAAAAAGGATT
AAAGCCATTAGAAGAAAAAGGAATTTTAGATTTACCAACCATACCAAATGGATATAAGCATAATGCACATATGTTTTACA
TTAAAACAAAAACAGAAGAAGACAGAAATGAACTTATTAAATACTTAAAGGAACAAGAAATTATGGCTGTAACCCATTAT
GTTCCATTACACCAATCTGAAGCTGGTAGATATTATGGATCCTTCTCAGGAGAAGACATGTATACAACATCTCATAGTAA
CAGGATTCTACGCTTACCACTTTATTACGGAATAACAAAAAGGGATGTCGCATATGTCATACAAACAATTCAGCAATATT
ATTCAACATAA

Upstream 100 bases:

>100_bases
TTTATACTATCGGCAATATTCTTTGGAGAACCAATCAGTACACAGAAAATAGTTGGATTTGGATTAATTATACTAGGAAT
TATAGTAATGAGGTGATCAG

Downstream 100 bases:

>100_bases
ATGGCTGTGGCTTGGTGTATTAATTATTCTTGCGTACCTATCACCATATTTTATAAATGGAGAAAACACGCATCTACGAA
TACATGACAATATGGATTCA

Product: TDP-4-oxo-6-deoxy-D-glucose transaminase

Products: TDP-D-fucosamine; 2-oxoglutarate [C]

Alternate protein names: NA

Number of amino acids: Translated: 376; Mature: 376

Protein sequence:

>376_residues
MIPFNIPCHLGQEEEAIAEAIKEQKLSGNGRFSRYCTTWLENQLDAKKAILTPSCTAALEMSAILTDVSEGDEVIMPSYT
FVSTANAFALRGAHIRFVDVEPTTMNIDPKAIEAAINHRTKAIVVVHYGGVACDMIPIMNLANEHQLWVIEDAAQGLKSQ
YNGRPLGTIGHLGTFSFHETKNYTCGEGGALIINDDAFIERAEIIQEKGTDRSKFVRGEVDKYTWRDIGSSYLTSELNAA
YLSVQLDQAEVINQDRLATWYLYQKGLKPLEEKGILDLPTIPNGYKHNAHMFYIKTKTEEDRNELIKYLKEQEIMAVTHY
VPLHQSEAGRYYGSFSGEDMYTTSHSNRILRLPLYYGITKRDVAYVIQTIQQYYST

Sequences:

>Translated_376_residues
MIPFNIPCHLGQEEEAIAEAIKEQKLSGNGRFSRYCTTWLENQLDAKKAILTPSCTAALEMSAILTDVSEGDEVIMPSYT
FVSTANAFALRGAHIRFVDVEPTTMNIDPKAIEAAINHRTKAIVVVHYGGVACDMIPIMNLANEHQLWVIEDAAQGLKSQ
YNGRPLGTIGHLGTFSFHETKNYTCGEGGALIINDDAFIERAEIIQEKGTDRSKFVRGEVDKYTWRDIGSSYLTSELNAA
YLSVQLDQAEVINQDRLATWYLYQKGLKPLEEKGILDLPTIPNGYKHNAHMFYIKTKTEEDRNELIKYLKEQEIMAVTHY
VPLHQSEAGRYYGSFSGEDMYTTSHSNRILRLPLYYGITKRDVAYVIQTIQQYYST
>Mature_376_residues
MIPFNIPCHLGQEEEAIAEAIKEQKLSGNGRFSRYCTTWLENQLDAKKAILTPSCTAALEMSAILTDVSEGDEVIMPSYT
FVSTANAFALRGAHIRFVDVEPTTMNIDPKAIEAAINHRTKAIVVVHYGGVACDMIPIMNLANEHQLWVIEDAAQGLKSQ
YNGRPLGTIGHLGTFSFHETKNYTCGEGGALIINDDAFIERAEIIQEKGTDRSKFVRGEVDKYTWRDIGSSYLTSELNAA
YLSVQLDQAEVINQDRLATWYLYQKGLKPLEEKGILDLPTIPNGYKHNAHMFYIKTKTEEDRNELIKYLKEQEIMAVTHY
VPLHQSEAGRYYGSFSGEDMYTTSHSNRILRLPLYYGITKRDVAYVIQTIQQYYST

Specific function: Involved in ECA elongation [H]

COG id: COG0399

COG function: function code M; Predicted pyridoxal phosphate-dependent enzyme apparently involved in regulation of cell wall biogenesis

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the degT/dnrJ/eryC1 family [H]

Homologues:

Organism=Escherichia coli, GI2367285, Length=376, Percent_Identity=60.9042553191489, Blast_Score=473, Evalue=1e-134,
Organism=Escherichia coli, GI145693159, Length=395, Percent_Identity=32.1518987341772, Blast_Score=157, Evalue=1e-39,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR000653
- InterPro:   IPR012749
- InterPro:   IPR015424
- InterPro:   IPR015421
- InterPro:   IPR015422 [H]

Pfam domain/function: PF01041 DegT_DnrJ_EryC1 [H]

EC number: NA

Molecular weight: Translated: 42476; Mature: 42476

Theoretical pI: Translated: 5.59; Mature: 5.59

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.3 %Cys     (Translated Protein)
2.4 %Met     (Translated Protein)
3.7 %Cys+Met (Translated Protein)
1.3 %Cys     (Mature Protein)
2.4 %Met     (Mature Protein)
3.7 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MIPFNIPCHLGQEEEAIAEAIKEQKLSGNGRFSRYCTTWLENQLDAKKAILTPSCTAALE
CCCCCCCCCCCCHHHHHHHHHHHHHCCCCCCHHHHHHHHHHHHHCHHHEECCCCHHHHHH
MSAILTDVSEGDEVIMPSYTFVSTANAFALRGAHIRFVDVEPTTMNIDPKAIEAAINHRT
HHHHHHHCCCCCCEEECCEEEEECCCEEEEECCEEEEEECCCCEECCCHHHHHHHHCCCC
KAIVVVHYGGVACDMIPIMNLANEHQLWVIEDAAQGLKSQYNGRPLGTIGHLGTFSFHET
EEEEEEEECCCHHHHHHHHCCCCCCEEEEEHHHHHHHHHHCCCCCCCCCCCCCCEEEECC
KNYTCGEGGALIINDDAFIERAEIIQEKGTDRSKFVRGEVDKYTWRDIGSSYLTSELNAA
CCCCCCCCCEEEECCHHHHHHHHHHHHCCCCHHHHHCCCCCCHHHHHCCHHHHHHHCCEE
YLSVQLDQAEVINQDRLATWYLYQKGLKPLEEKGILDLPTIPNGYKHNAHMFYIKTKTEE
EEEEEECHHHHCCCCCCEEHHHHHHCCCCHHHCCCEECCCCCCCCCCCCEEEEEEECCCC
DRNELIKYLKEQEIMAVTHYVPLHQSEAGRYYGSFSGEDMYTTSHSNRILRLPLYYGITK
HHHHHHHHHHHCCEEEEEEEECCCCCCCCCEECCCCCCCEEEECCCCEEEEEEHHHCCCH
RDVAYVIQTIQQYYST
HHHHHHHHHHHHHHCC
>Mature Secondary Structure
MIPFNIPCHLGQEEEAIAEAIKEQKLSGNGRFSRYCTTWLENQLDAKKAILTPSCTAALE
CCCCCCCCCCCCHHHHHHHHHHHHHCCCCCCHHHHHHHHHHHHHCHHHEECCCCHHHHHH
MSAILTDVSEGDEVIMPSYTFVSTANAFALRGAHIRFVDVEPTTMNIDPKAIEAAINHRT
HHHHHHHCCCCCCEEECCEEEEECCCEEEEECCEEEEEECCCCEECCCHHHHHHHHCCCC
KAIVVVHYGGVACDMIPIMNLANEHQLWVIEDAAQGLKSQYNGRPLGTIGHLGTFSFHET
EEEEEEEECCCHHHHHHHHCCCCCCEEEEEHHHHHHHHHHCCCCCCCCCCCCCCEEEECC
KNYTCGEGGALIINDDAFIERAEIIQEKGTDRSKFVRGEVDKYTWRDIGSSYLTSELNAA
CCCCCCCCCEEEECCHHHHHHHHHHHHCCCCHHHHHCCCCCCHHHHHCCHHHHHHHCCEE
YLSVQLDQAEVINQDRLATWYLYQKGLKPLEEKGILDLPTIPNGYKHNAHMFYIKTKTEE
EEEEEECHHHHCCCCCCEEHHHHHHCCCCHHHCCCEECCCCCCCCCCCCEEEEEEECCCC
DRNELIKYLKEQEIMAVTHYVPLHQSEAGRYYGSFSGEDMYTTSHSNRILRLPLYYGITK
HHHHHHHHHHHCCEEEEEEEECCCCCCCCCEECCCCCCCEEEECCCCEEEEEEHHHCCCH
RDVAYVIQTIQQYYST
HHHHHHHHHHHHHHCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: dTDP-4-dehydro-6-deoxy-D-glucose; L-glutamate [C]

Specific reaction: dTDP-4-dehydro-6-deoxy-D-glucose + L-glutamate = TDP-D-fucosamine + 2-oxoglutarate [C]

General reaction: Carbon-Oxygen Lyases; Hydro-Lyases [C]

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 1379743; 9278503; 8366065 [H]