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The map label for this gene is prfC [H]

Identifier: 23099745

GI number: 23099745

Start: 2334520

End: 2336091

Strand: Direct

Name: prfC [H]

Synonym: OB2290

Alternate gene names: 23099745

Gene position: 2334520-2336091 (Clockwise)

Preceding gene: 23099733

Following gene: 23099746

Centisome position: 64.3

GC content: 36.13

Gene sequence:

>1572_bases
ATGTCGATAAATAATGAAGTATCAAAACGAAAAACGTTTGCAATTATATCTCACCCAGATGCCGGGAAGACAACGATGAC
GGAAAAACTATTGTTGTACGGAAATTTAATACGTTCAGCAGGTACTGTAAAAGGAAAAAAATCTGGTAAATTCGCAACCT
CAGATTGGATGGAAATTGAAAAACAACGTGGGATTTCTGTTACATCTAGTGTAATGAATTTCACTTATGATGATTTGCAT
ATCAATATTCTTGATACACCTGGTCATGAAGATTTTAGTGAAGATACCTATCGTACATTAACAGCAGTCGATAGCGTGGT
TATGATTATTGACTCTACAAAAGGTATCGAGGCTCAAACCATTAAATTATTCAAAGTTTGTCGGATGCGAGGTATTCCCA
TTTTCACTTTTATGAACAAATTGGATCGCGAAGGAAAGGAACCCCTTGCTCTTTTAGAAGAACTTGAAGAAGTCTTAGAA
ATTGACACCTATCCAATGAATTGGCCAGCAGGTATGGGAAAAGGTTTCTTAGGAATTTTTGATAGACATGGAGAGCAATT
TGTTCAGCATCATCCTGGTGATACAGAGACGTATATTCCAAACAACGAATTATCTAATCATAAAGATCTTGTAGAAAACC
CAACATTTAAAGATGCACAACAAGAATTAGAATTAGTAACGGAAGCGGGAAATGAGTATTCTGAAGAAGCTATTTTGCGC
GGGGAATTAACACCTGTATTCTTCGGTAGTGCTTTATCTCCATTTGGAGTACAAGTGTTCTTTGATACATTTATTCATCT
TGCACCTAGCCCAACATCACGAAAAACGACAACGGGACCAATTAAACCAGACAATGAAGACTTTTCAGGGTTTATCTTTA
AAATTCAAGCAAACATGAATCCTGCCCATCGTGACCGAATTGCTTTCTTACGAGTATGCTCTGGTAAATTTGAACGTGGT
ATGACTGTTCGCTTAGCACGAACAGGTAAAGAACTAAAACTAGCACAGTCACAACAAATGGTCGCTTCTTCTAGAGATAC
AGTTGATGAAGCATATGCTGGAGATATTATAGGTGTATATGATCCAAATGCCTATCAAATCGGAGATACACTTCTAGTTG
GAAAAAACATGTATGAATACGATGAATTGCCACAATTCCCTCCGGAACTATTTAAAAAAGTTAGTGCAAAAAATGCTTTA
AAATCAAAACAGTTCCGAAAAGGAATAGAGCAGTTAGTTCAAGAAGGTGCTATTCAATTATTCCGCGATCAAATGACAGA
TGCTTTTATATTAGGTGCTGTAGGAGAATTGCAATATCAAGTCTTTACTTATCGGATGCAGCATGAATATAATGCAGAAG
TTATGCTGGAATCTATTGGTGAACGTATTCCTCGTTGGTTAAAAGAAGAACAAGTTGATAGAAAGTTATTTGATAGTGGT
AATTTACTTGTTAGAGACCGAAACAATCAACCACTTGTGTTATTCCGAAATGAATTTGCGTTGAACTGGTTTAAGGACAA
AAATCCTGAAGTCGACTTAATTGATCTTTTCGAAGTTAATGATTACACATAA

Upstream 100 bases:

>100_bases
TATAAAATTCTATAGTATAGTATGGAGGTGTCTCTTTATTATAAAACATCTGAATAAGAACATCTATTTGTATATAGAAA
TGAAAGAAGGTTAAGACGGT

Downstream 100 bases:

>100_bases
GTTTCCATTACATGTTGGGATAAAATTCCCATTCATGATTATAGATCATTCTTTGCATAATAAGGTTATCTTTAATCAAA
AGGAGTGGAAACTTATGGGA

Product: peptide chain release factor RF-3

Products: NA

Alternate protein names: RF-3 [H]

Number of amino acids: Translated: 523; Mature: 522

Protein sequence:

>523_residues
MSINNEVSKRKTFAIISHPDAGKTTMTEKLLLYGNLIRSAGTVKGKKSGKFATSDWMEIEKQRGISVTSSVMNFTYDDLH
INILDTPGHEDFSEDTYRTLTAVDSVVMIIDSTKGIEAQTIKLFKVCRMRGIPIFTFMNKLDREGKEPLALLEELEEVLE
IDTYPMNWPAGMGKGFLGIFDRHGEQFVQHHPGDTETYIPNNELSNHKDLVENPTFKDAQQELELVTEAGNEYSEEAILR
GELTPVFFGSALSPFGVQVFFDTFIHLAPSPTSRKTTTGPIKPDNEDFSGFIFKIQANMNPAHRDRIAFLRVCSGKFERG
MTVRLARTGKELKLAQSQQMVASSRDTVDEAYAGDIIGVYDPNAYQIGDTLLVGKNMYEYDELPQFPPELFKKVSAKNAL
KSKQFRKGIEQLVQEGAIQLFRDQMTDAFILGAVGELQYQVFTYRMQHEYNAEVMLESIGERIPRWLKEEQVDRKLFDSG
NLLVRDRNNQPLVLFRNEFALNWFKDKNPEVDLIDLFEVNDYT

Sequences:

>Translated_523_residues
MSINNEVSKRKTFAIISHPDAGKTTMTEKLLLYGNLIRSAGTVKGKKSGKFATSDWMEIEKQRGISVTSSVMNFTYDDLH
INILDTPGHEDFSEDTYRTLTAVDSVVMIIDSTKGIEAQTIKLFKVCRMRGIPIFTFMNKLDREGKEPLALLEELEEVLE
IDTYPMNWPAGMGKGFLGIFDRHGEQFVQHHPGDTETYIPNNELSNHKDLVENPTFKDAQQELELVTEAGNEYSEEAILR
GELTPVFFGSALSPFGVQVFFDTFIHLAPSPTSRKTTTGPIKPDNEDFSGFIFKIQANMNPAHRDRIAFLRVCSGKFERG
MTVRLARTGKELKLAQSQQMVASSRDTVDEAYAGDIIGVYDPNAYQIGDTLLVGKNMYEYDELPQFPPELFKKVSAKNAL
KSKQFRKGIEQLVQEGAIQLFRDQMTDAFILGAVGELQYQVFTYRMQHEYNAEVMLESIGERIPRWLKEEQVDRKLFDSG
NLLVRDRNNQPLVLFRNEFALNWFKDKNPEVDLIDLFEVNDYT
>Mature_522_residues
SINNEVSKRKTFAIISHPDAGKTTMTEKLLLYGNLIRSAGTVKGKKSGKFATSDWMEIEKQRGISVTSSVMNFTYDDLHI
NILDTPGHEDFSEDTYRTLTAVDSVVMIIDSTKGIEAQTIKLFKVCRMRGIPIFTFMNKLDREGKEPLALLEELEEVLEI
DTYPMNWPAGMGKGFLGIFDRHGEQFVQHHPGDTETYIPNNELSNHKDLVENPTFKDAQQELELVTEAGNEYSEEAILRG
ELTPVFFGSALSPFGVQVFFDTFIHLAPSPTSRKTTTGPIKPDNEDFSGFIFKIQANMNPAHRDRIAFLRVCSGKFERGM
TVRLARTGKELKLAQSQQMVASSRDTVDEAYAGDIIGVYDPNAYQIGDTLLVGKNMYEYDELPQFPPELFKKVSAKNALK
SKQFRKGIEQLVQEGAIQLFRDQMTDAFILGAVGELQYQVFTYRMQHEYNAEVMLESIGERIPRWLKEEQVDRKLFDSGN
LLVRDRNNQPLVLFRNEFALNWFKDKNPEVDLIDLFEVNDYT

Specific function: Increases the formation of ribosomal termination complexes and stimulates activities of RF-1 and RF-2. It binds guanine nucleotides and has strong preference for UGA stop codons. It may interact directly with the ribosome. The stimulation of RF- 1 and RF-

COG id: COG4108

COG function: function code J; Peptide chain release factor RF-3

Gene ontology:

Cell location: Cytoplasm [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the GTP-binding elongation factor family. PrfC subfamily [H]

Homologues:

Organism=Homo sapiens, GI18390331, Length=482, Percent_Identity=28.4232365145228, Blast_Score=187, Evalue=3e-47,
Organism=Homo sapiens, GI19923640, Length=567, Percent_Identity=24.3386243386243, Blast_Score=129, Evalue=5e-30,
Organism=Homo sapiens, GI25306283, Length=356, Percent_Identity=28.0898876404494, Blast_Score=126, Evalue=6e-29,
Organism=Homo sapiens, GI25306287, Length=307, Percent_Identity=29.6416938110749, Blast_Score=120, Evalue=4e-27,
Organism=Homo sapiens, GI157426893, Length=155, Percent_Identity=30.3225806451613, Blast_Score=90, Evalue=4e-18,
Organism=Homo sapiens, GI94966754, Length=137, Percent_Identity=29.1970802919708, Blast_Score=78, Evalue=2e-14,
Organism=Homo sapiens, GI4503483, Length=158, Percent_Identity=26.5822784810127, Blast_Score=72, Evalue=2e-12,
Organism=Homo sapiens, GI310132016, Length=113, Percent_Identity=30.9734513274336, Blast_Score=69, Evalue=1e-11,
Organism=Homo sapiens, GI310110807, Length=113, Percent_Identity=30.9734513274336, Blast_Score=69, Evalue=1e-11,
Organism=Homo sapiens, GI310123363, Length=113, Percent_Identity=30.9734513274336, Blast_Score=69, Evalue=1e-11,
Organism=Escherichia coli, GI1790835, Length=478, Percent_Identity=49.7907949790795, Blast_Score=472, Evalue=1e-134,
Organism=Escherichia coli, GI1789738, Length=516, Percent_Identity=27.1317829457364, Blast_Score=169, Evalue=6e-43,
Organism=Escherichia coli, GI48994988, Length=142, Percent_Identity=34.5070422535211, Blast_Score=92, Evalue=7e-20,
Organism=Escherichia coli, GI1788922, Length=179, Percent_Identity=27.3743016759777, Blast_Score=90, Evalue=3e-19,
Organism=Caenorhabditis elegans, GI17533571, Length=484, Percent_Identity=27.0661157024793, Blast_Score=184, Evalue=1e-46,
Organism=Caenorhabditis elegans, GI17556745, Length=196, Percent_Identity=31.6326530612245, Blast_Score=108, Evalue=5e-24,
Organism=Caenorhabditis elegans, GI17557151, Length=154, Percent_Identity=29.2207792207792, Blast_Score=87, Evalue=3e-17,
Organism=Caenorhabditis elegans, GI71988819, Length=133, Percent_Identity=30.0751879699248, Blast_Score=75, Evalue=7e-14,
Organism=Caenorhabditis elegans, GI71988811, Length=133, Percent_Identity=30.0751879699248, Blast_Score=75, Evalue=8e-14,
Organism=Saccharomyces cerevisiae, GI6323098, Length=482, Percent_Identity=26.3485477178423, Blast_Score=176, Evalue=1e-44,
Organism=Saccharomyces cerevisiae, GI6322359, Length=511, Percent_Identity=26.027397260274, Blast_Score=140, Evalue=7e-34,
Organism=Saccharomyces cerevisiae, GI6323320, Length=138, Percent_Identity=27.536231884058, Blast_Score=78, Evalue=3e-15,
Organism=Saccharomyces cerevisiae, GI6324707, Length=169, Percent_Identity=26.6272189349112, Blast_Score=74, Evalue=6e-14,
Organism=Saccharomyces cerevisiae, GI6320593, Length=169, Percent_Identity=26.6272189349112, Blast_Score=74, Evalue=6e-14,
Organism=Drosophila melanogaster, GI24582462, Length=480, Percent_Identity=27.7083333333333, Blast_Score=180, Evalue=2e-45,
Organism=Drosophila melanogaster, GI221458488, Length=499, Percent_Identity=25.0501002004008, Blast_Score=140, Evalue=2e-33,
Organism=Drosophila melanogaster, GI78706572, Length=182, Percent_Identity=30.7692307692308, Blast_Score=101, Evalue=1e-21,
Organism=Drosophila melanogaster, GI24585711, Length=163, Percent_Identity=28.8343558282209, Blast_Score=73, Evalue=4e-13,
Organism=Drosophila melanogaster, GI24585713, Length=163, Percent_Identity=28.8343558282209, Blast_Score=73, Evalue=4e-13,
Organism=Drosophila melanogaster, GI24585709, Length=163, Percent_Identity=28.8343558282209, Blast_Score=73, Evalue=4e-13,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR009022
- InterPro:   IPR004548
- InterPro:   IPR000795
- InterPro:   IPR005225
- InterPro:   IPR004161
- InterPro:   IPR009000 [H]

Pfam domain/function: PF00009 GTP_EFTU; PF03144 GTP_EFTU_D2 [H]

EC number: NA

Molecular weight: Translated: 59550; Mature: 59419

Theoretical pI: Translated: 4.95; Mature: 4.95

Prosite motif: PS00732 RIBOSOMAL_S16 ; PS00301 EFACTOR_GTP

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.4 %Cys     (Translated Protein)
3.1 %Met     (Translated Protein)
3.4 %Cys+Met (Translated Protein)
0.4 %Cys     (Mature Protein)
2.9 %Met     (Mature Protein)
3.3 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MSINNEVSKRKTFAIISHPDAGKTTMTEKLLLYGNLIRSAGTVKGKKSGKFATSDWMEIE
CCCCCCHHCCEEEEEEECCCCCCHHHHHHHHHHHHHHHHCCCCCCCCCCCCCCHHHHHHH
KQRGISVTSSVMNFTYDDLHINILDTPGHEDFSEDTYRTLTAVDSVVMIIDSTKGIEAQT
HHCCCHHHHHHHHCEECCEEEEEEECCCCCCCCHHHHHHHHHHHHEEEEEECCCCCCHHH
IKLFKVCRMRGIPIFTFMNKLDREGKEPLALLEELEEVLEIDTYPMNWPAGMGKGFLGIF
HHHHHHHHHCCCHHHHHHHHHCCCCCCHHHHHHHHHHHHHCCCCCCCCCCCCCCCHHHHH
DRHGEQFVQHHPGDTETYIPNNELSNHKDLVENPTFKDAQQELELVTEAGNEYSEEAILR
HHHHHHHHHCCCCCCCCCCCCCCCCCCHHHHCCCCHHHHHHHHHHHHHCCCCHHHHHHHC
GELTPVFFGSALSPFGVQVFFDTFIHLAPSPTSRKTTTGPIKPDNEDFSGFIFKIQANMN
CCCCCEEECCCCCCCHHHHHHHHHHHCCCCCCCCCCCCCCCCCCCCCCCEEEEEEEECCC
PAHRDRIAFLRVCSGKFERGMTVRLARTGKELKLAQSQQMVASSRDTVDEAYAGDIIGVY
HHHHHHHHHHHHHCCHHCCCCEEEEECCCCHHHHHHHHHHHHHCCCHHHHHHCCCEEEEE
DPNAYQIGDTLLVGKNMYEYDELPQFPPELFKKVSAKNALKSKQFRKGIEQLVQEGAIQL
CCCCEECCCEEEECCCCHHHHCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
FRDQMTDAFILGAVGELQYQVFTYRMQHEYNAEVMLESIGERIPRWLKEEQVDRKLFDSG
HHHHHHHEEEHHHHHHHEEEEEEEECCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHCCC
NLLVRDRNNQPLVLFRNEFALNWFKDKNPEVDLIDLFEVNDYT
CEEEEECCCCCEEEEECCEEEEEECCCCCCEEEEEEEECCCCC
>Mature Secondary Structure 
SINNEVSKRKTFAIISHPDAGKTTMTEKLLLYGNLIRSAGTVKGKKSGKFATSDWMEIE
CCCCCHHCCEEEEEEECCCCCCHHHHHHHHHHHHHHHHCCCCCCCCCCCCCCHHHHHHH
KQRGISVTSSVMNFTYDDLHINILDTPGHEDFSEDTYRTLTAVDSVVMIIDSTKGIEAQT
HHCCCHHHHHHHHCEECCEEEEEEECCCCCCCCHHHHHHHHHHHHEEEEEECCCCCCHHH
IKLFKVCRMRGIPIFTFMNKLDREGKEPLALLEELEEVLEIDTYPMNWPAGMGKGFLGIF
HHHHHHHHHCCCHHHHHHHHHCCCCCCHHHHHHHHHHHHHCCCCCCCCCCCCCCCHHHHH
DRHGEQFVQHHPGDTETYIPNNELSNHKDLVENPTFKDAQQELELVTEAGNEYSEEAILR
HHHHHHHHHCCCCCCCCCCCCCCCCCCHHHHCCCCHHHHHHHHHHHHHCCCCHHHHHHHC
GELTPVFFGSALSPFGVQVFFDTFIHLAPSPTSRKTTTGPIKPDNEDFSGFIFKIQANMN
CCCCCEEECCCCCCCHHHHHHHHHHHCCCCCCCCCCCCCCCCCCCCCCCEEEEEEEECCC
PAHRDRIAFLRVCSGKFERGMTVRLARTGKELKLAQSQQMVASSRDTVDEAYAGDIIGVY
HHHHHHHHHHHHHCCHHCCCCEEEEECCCCHHHHHHHHHHHHHCCCHHHHHHCCCEEEEE
DPNAYQIGDTLLVGKNMYEYDELPQFPPELFKKVSAKNALKSKQFRKGIEQLVQEGAIQL
CCCCEECCCEEEECCCCHHHHCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
FRDQMTDAFILGAVGELQYQVFTYRMQHEYNAEVMLESIGERIPRWLKEEQVDRKLFDSG
HHHHHHHEEEHHHHHHHEEEEEEEECCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHCCC
NLLVRDRNNQPLVLFRNEFALNWFKDKNPEVDLIDLFEVNDYT
CEEEEECCCCCEEEEECCEEEEEECCCCCCEEEEEEEECCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 11679669 [H]