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The map label for this gene is yjbJ [H]

Identifier: 23099593

GI number: 23099593

Start: 2174701

End: 2175378

Strand: Direct

Name: yjbJ [H]

Synonym: OB2138

Alternate gene names: 23099593

Gene position: 2174701-2175378 (Clockwise)

Preceding gene: 23099592

Following gene: 23099600

Centisome position: 59.9

GC content: 33.04

Gene sequence:

>678_bases
TTGCCTATTTTTTTACAAACCGCTAAAATAGAATTAATAGATTATAAAAACGGATGTGATGAAGTGGAAATCAATGGTCT
TCCGCAAATGATGCCTTTTCAAATAATGTCATCTTTACAAACAAGTAATTCTACTGATTCTTTAGGTGTATCGTTTCAAC
AATTATTATTAGAAAAAATTAACGAAGCAGAAAGACGTACTAGTTTGGATTTTGGGTCAAATTTAGTATCTGTTCCATAT
AAAATGCAATCCCCTCTGAATGCAATAGACAATTCATCTATAACTACGAATAACTCTAGTGTGTCAGCTTATCAACAATT
AATCTCAAACGCGAGTCAAAAGTACGGAGTGGATGAAAGTCTAATTAATGCAGTAATTAAACATGAATCTAATTATAACC
CAAATGCTACTAGTTCAGCAGGTGCTCAAGGATTAATGCAATTGATGCCACAAACTGCAGCAGGTTTAGGAGTTACAAAT
GCTTATGATCCTGTTCAAAATATTAATGCAGGAACTAAATATCTTAGTCAGATGTTACAACGCTATAATGGCGATAATCA
ATTAGCATTAGCTGCTTATAATGCAGGTCCAGGAAATGTAGATAAATATCAAGGAATTCCTCCATTTCGTGAAACTACTG
CATATGTATCCAAAGTAATGCAATCCTTACAAGCATAA

Upstream 100 bases:

>100_bases
AAATCATTTCACTATGTGATTGGGTTATATGCATAGGTAAGGATAGTTTTTATGAAAATCTATTAAAATCAAGAGGAAAG
GAAATATTTACTATTTAATA

Downstream 100 bases:

>100_bases
TTAGATAAATTATAGAAAAAAATCATTAGCTGTCCATTTTTCGAGAAATTGGACAGCTTTTTTGTGGTCTAGGAAATAAA
ATATTACAGCAGTGGATATA

Product: lytic transglycosylase

Products: N-Acetylmuramic Acid Residues; N-Acetylglucosamine Residues [C]

Alternate protein names: NA

Number of amino acids: Translated: 225; Mature: 224

Protein sequence:

>225_residues
MPIFLQTAKIELIDYKNGCDEVEINGLPQMMPFQIMSSLQTSNSTDSLGVSFQQLLLEKINEAERRTSLDFGSNLVSVPY
KMQSPLNAIDNSSITTNNSSVSAYQQLISNASQKYGVDESLINAVIKHESNYNPNATSSAGAQGLMQLMPQTAAGLGVTN
AYDPVQNINAGTKYLSQMLQRYNGDNQLALAAYNAGPGNVDKYQGIPPFRETTAYVSKVMQSLQA

Sequences:

>Translated_225_residues
MPIFLQTAKIELIDYKNGCDEVEINGLPQMMPFQIMSSLQTSNSTDSLGVSFQQLLLEKINEAERRTSLDFGSNLVSVPY
KMQSPLNAIDNSSITTNNSSVSAYQQLISNASQKYGVDESLINAVIKHESNYNPNATSSAGAQGLMQLMPQTAAGLGVTN
AYDPVQNINAGTKYLSQMLQRYNGDNQLALAAYNAGPGNVDKYQGIPPFRETTAYVSKVMQSLQA
>Mature_224_residues
PIFLQTAKIELIDYKNGCDEVEINGLPQMMPFQIMSSLQTSNSTDSLGVSFQQLLLEKINEAERRTSLDFGSNLVSVPYK
MQSPLNAIDNSSITTNNSSVSAYQQLISNASQKYGVDESLINAVIKHESNYNPNATSSAGAQGLMQLMPQTAAGLGVTNA
YDPVQNINAGTKYLSQMLQRYNGDNQLALAAYNAGPGNVDKYQGIPPFRETTAYVSKVMQSLQA

Specific function: Murein-Degrading Enzyme. May Play A Role In Recycling Of Muropeptides During Cell Elongation And/Or Cell Division (By Similarity). [C]

COG id: COG0741

COG function: function code M; Soluble lytic murein transglycosylase and related regulatory proteins (some contain LysM/invasin domains)

Gene ontology:

Cell location: Attached To The Membrane By A Lipid Anchor [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the transglycosylase slt family [H]

Homologues:

Organism=Escherichia coli, GI87082441, Length=141, Percent_Identity=34.0425531914894, Blast_Score=71, Evalue=5e-14,
Organism=Escherichia coli, GI87082191, Length=116, Percent_Identity=39.6551724137931, Blast_Score=69, Evalue=2e-13,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR008258
- InterPro:   IPR000189 [H]

Pfam domain/function: PF01464 SLT [H]

EC number: 3.2.1.- [C]

Molecular weight: Translated: 24463; Mature: 24332

Theoretical pI: Translated: 4.56; Mature: 4.56

Prosite motif: PS00922 TRANSGLYCOSYLASE

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.4 %Cys     (Translated Protein)
4.0 %Met     (Translated Protein)
4.4 %Cys+Met (Translated Protein)
0.4 %Cys     (Mature Protein)
3.6 %Met     (Mature Protein)
4.0 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MPIFLQTAKIELIDYKNGCDEVEINGLPQMMPFQIMSSLQTSNSTDSLGVSFQQLLLEKI
CCEEEEECEEEEEECCCCCCEEEECCCCCHHHHHHHHHHHCCCCCCHHHHHHHHHHHHHH
NEAERRTSLDFGSNLVSVPYKMQSPLNAIDNSSITTNNSSVSAYQQLISNASQKYGVDES
HHHHHHHHHHHCCCEEECCHHHHCCHHHHCCCCCCCCCCHHHHHHHHHHHHHHHCCCCHH
LINAVIKHESNYNPNATSSAGAQGLMQLMPQTAAGLGVTNAYDPVQNINAGTKYLSQMLQ
HHHHHHHHCCCCCCCCCCHHHHHHHHHHHHHHHHCCCCCCCCCHHHHCCHHHHHHHHHHH
RYNGDNQLALAAYNAGPGNVDKYQGIPPFRETTAYVSKVMQSLQA
HHCCCCCEEEEEECCCCCCCCCCCCCCCHHHHHHHHHHHHHHHCC
>Mature Secondary Structure 
PIFLQTAKIELIDYKNGCDEVEINGLPQMMPFQIMSSLQTSNSTDSLGVSFQQLLLEKI
CEEEEECEEEEEECCCCCCEEEECCCCCHHHHHHHHHHHCCCCCCHHHHHHHHHHHHHH
NEAERRTSLDFGSNLVSVPYKMQSPLNAIDNSSITTNNSSVSAYQQLISNASQKYGVDES
HHHHHHHHHHHCCCEEECCHHHHCCHHHHCCCCCCCCCCHHHHHHHHHHHHHHHCCCCHH
LINAVIKHESNYNPNATSSAGAQGLMQLMPQTAAGLGVTNAYDPVQNINAGTKYLSQMLQ
HHHHHHHHCCCCCCCCCCHHHHHHHHHHHHHHHHCCCCCCCCCHHHHCCHHHHHHHHHHH
RYNGDNQLALAAYNAGPGNVDKYQGIPPFRETTAYVSKVMQSLQA
HHCCCCCEEEEEECCCCCCCCCCCCCCCHHHHHHHHHHHHHHHCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: Muramic Acid [C]

Specific reaction: Cleavage Of The Beta-1,4-Glycosidic Bond Between N-Acetylmuramic Acid And N-Acetylglucosamine Residues, Thereby Conserving The Energy In A Newly Synthesized 1,6-Anhydrobond In The Muramic Acid Residue. [C]

General reaction: Cleavage Of The Beta-1,4-Glycosidic Bond [C]

Inhibitor: NA

Structure determination priority: 7.0

TargetDB status: NA

Availability: NA

References: 9384377 [H]