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The map label for this gene is mrpA [H]

Identifier: 23099446

GI number: 23099446

Start: 2016495

End: 2018912

Strand: Direct

Name: mrpA [H]

Synonym: OB1991

Alternate gene names: 23099446

Gene position: 2016495-2018912 (Clockwise)

Preceding gene: 23099433

Following gene: 23099447

Centisome position: 55.54

GC content: 34.99

Gene sequence:

>2418_bases
TTGACAGTTTTACATTGGGCTACTCTATCCCCCTTTTTGATAGCCATTCTTATTCCGTTTTTGTATAAATATGTAAGGAA
AGTGCATACTGGCTGGTTTGTTTTACTCTTGCCTTTTACTTTATTTATCTATTTTCTTTCGTTTTTATCAAGTACTAGAG
AAGGAAATGTAATTTCTGAACATCTTGCATGGGTACCTTCCCTTGGTATCAATTTTAATTTATACATAGATGGTCTTAGT
CTATTATTTGCTTTATTAATTACTGGGATTGGTACATTAGTTGTACTTTATTCCATATTTTATCTCTCAGATATTAAAGA
GAAGCTGAACAATTTTTATGTATATCTCTTAATGTTTATGGGAGCAATGTTAGGAGTCGTTTTATCCGATAACCTAATAG
TTATCTATGTGTTTTGGGAAGTAACTAGTATTGCTTCGTCCTTGTTAATTAGTTATTGGTATGGCAGAGAAAAATCAATA
TACGGTGCTCAAAAATCAATGTATATCACTGTATTTGGTGGTTTGTCGATGCTTGGAGGATTCTCCCTTTTATATGTAAT
CGCTGGTACATTCAGTATTCGTGAATTAATTGCAAATGCAGATATTGTTATGGCAAGTTCACTATTTCTACCTGCAATGA
TTCTTGTATTATTAGGTGCATTTACAAAATCTGCTCAGTTTCCTTTTCATATCTGGCTACCAGATGCAATGGAAGCTCCA
ACCCCAGTTAGTGCTTATCTTCACTCCGCTACAATGGTAAAAGCGGGGATTTACCTTGTCGCTCGATTAACAGGTGTGTT
CGGAGGAAGCCCTGAGTGGTTCTGGATAATTACAATTGCTGGACTAACTACATTGGCGTGGGGATCTATATCCGCTGTTC
GTCAAAAAGATTTAAAAGGTATATTAGCCTTTTCGACTATTAGTCAATTAGGCTTAATTATGAGTTTACTTGGCGTCGGT
TCTGCTGCATTCTACTTCCAAGATGGTAATAATGCATTATATACTACTGCTATTTTAGCAGCAGTATTTCACTTAATTAA
CCATGCTACCTTCAAAGGAAGCTTATTTATGGCTGTAGGTATCATTGATCATGAAACTGGTACAAGAGATATAAGAAATC
TCGGTGGTTTAATGACCATTATGCCTATTACAGCAACTGTCTCTCTAGTTGGATTAGCTTCAATGGCTGGATTACCACCA
TTCAATGGGTTCTTAAGTAAAGAATTGTTCTTTACAGGTATGTTGAATGCAACGGAATACGGTATATTTAATCTAGAAAC
AATGGGATATATCATACCTATTGTCGCTTGGATCGCTAGCGTTTTTACTTTCTTATACTGTATGATTATGTTCTTCCGTA
CATTTACTGGTAAATTTGATGGAAGCAAACTAAAAGTAGACCATGTACATGAAGCTCCAATTGGAATGCTAATAAGTCCT
GTTATTTTAGGAATTCTAGTTATTTTATTTGGTCTATTCCCAAATGTATTAGCTTATTCGTTAATAGAGCCTGCGATGGC
TGGTATTTTGCCAGGAATTTTAAACCCAGGTGAGTTTTTCAATGTTCACATTACACATTGGCATGGTTTTATACCAGAAC
TCTTTATGACAATTGGTGTGATCGTTCTTGGAGTAATTTTATACCTGTCAATGAGTAAATGGCAGAAAACTGCTTTTTAC
CTTGGTGAAAGAGACATCTTTAATTATGCCTATGACTATGGTTATGACGGATTAATTAAAGGATCGCAATGGATAACACG
CATTCAAATGACCGGGCGACTTCGGGACTATTTTATCTATATGTGTGGATTTATGATTCTTCTTATAGGATATACGATGT
ATAAGTTTAATCTTCCTTCGTTTAACACGATGGAACTTTCCCCAATCGAACCATTTATCTGGATAGTTGCTATCGTATTT
ATTGGTTCTGTTATTGCAGTACCATTTATCAATAATCGTCTTGGATTGATTATCCTTACAGGAGTAACCGGATATATTGT
AGCTCTATTTTTTGTTGTATTCCGTGCACCCGATTTAGCACTAACCCAATTACTTGTTGAAACTGTTACTGTAATTCTTT
TCATGCTTGCTTTTTACCACTTACCTAAATTGAAAAAAGAAAAAACAAGCAAAGGTATACATGCGATAAAGCTTATTATT
TCAATTTCGTTTGGAGTAATGATAACACTTGTATCATTAAGTGCATTTTCAATGGGTAGTACAGCTGGAATGCCATCTAT
TTCCGAGTACTTCACTGAATTCTCAAAAGAACTCGCAGGAGGATACAATATTGTAAATGTCATTCTAGTTGATTTTCGTG
GTTTAGATACTATGTTGGAGATTCTTGTTGTAGGGGTAGCTGTTTTATCAATAATCAGCCTTATAAAACTCCGTTTCAAA
GGGAGTGAAGATGTATGA

Upstream 100 bases:

>100_bases
GTATGGATGATTTCTTTCCATTGTGGAATCTTAATTAAAAAACCAAAAGATAACTAAATAAAAAAATATGCTTTTTATGT
TAGAAAAGGAGGAATTATCT

Downstream 100 bases:

>100_bases
AACAAGTAAAACCGAATGATGTTATGTTACAAACTTTTGCTCGTATGTTAATTTTTATAATACTTGCGTTCTCCATCTAC
CTACTGTTAGCCGGACATAA

Product: monovalent cation/H+ antiporter subunit A

Products: NAD+; ubiquinol [C]

Alternate protein names: Mrp complex subunit A; Multiple resistance and pH homeostasis protein A [H]

Number of amino acids: Translated: 805; Mature: 804

Protein sequence:

>805_residues
MTVLHWATLSPFLIAILIPFLYKYVRKVHTGWFVLLLPFTLFIYFLSFLSSTREGNVISEHLAWVPSLGINFNLYIDGLS
LLFALLITGIGTLVVLYSIFYLSDIKEKLNNFYVYLLMFMGAMLGVVLSDNLIVIYVFWEVTSIASSLLISYWYGREKSI
YGAQKSMYITVFGGLSMLGGFSLLYVIAGTFSIRELIANADIVMASSLFLPAMILVLLGAFTKSAQFPFHIWLPDAMEAP
TPVSAYLHSATMVKAGIYLVARLTGVFGGSPEWFWIITIAGLTTLAWGSISAVRQKDLKGILAFSTISQLGLIMSLLGVG
SAAFYFQDGNNALYTTAILAAVFHLINHATFKGSLFMAVGIIDHETGTRDIRNLGGLMTIMPITATVSLVGLASMAGLPP
FNGFLSKELFFTGMLNATEYGIFNLETMGYIIPIVAWIASVFTFLYCMIMFFRTFTGKFDGSKLKVDHVHEAPIGMLISP
VILGILVILFGLFPNVLAYSLIEPAMAGILPGILNPGEFFNVHITHWHGFIPELFMTIGVIVLGVILYLSMSKWQKTAFY
LGERDIFNYAYDYGYDGLIKGSQWITRIQMTGRLRDYFIYMCGFMILLIGYTMYKFNLPSFNTMELSPIEPFIWIVAIVF
IGSVIAVPFINNRLGLIILTGVTGYIVALFFVVFRAPDLALTQLLVETVTVILFMLAFYHLPKLKKEKTSKGIHAIKLII
SISFGVMITLVSLSAFSMGSTAGMPSISEYFTEFSKELAGGYNIVNVILVDFRGLDTMLEILVVGVAVLSIISLIKLRFK
GSEDV

Sequences:

>Translated_805_residues
MTVLHWATLSPFLIAILIPFLYKYVRKVHTGWFVLLLPFTLFIYFLSFLSSTREGNVISEHLAWVPSLGINFNLYIDGLS
LLFALLITGIGTLVVLYSIFYLSDIKEKLNNFYVYLLMFMGAMLGVVLSDNLIVIYVFWEVTSIASSLLISYWYGREKSI
YGAQKSMYITVFGGLSMLGGFSLLYVIAGTFSIRELIANADIVMASSLFLPAMILVLLGAFTKSAQFPFHIWLPDAMEAP
TPVSAYLHSATMVKAGIYLVARLTGVFGGSPEWFWIITIAGLTTLAWGSISAVRQKDLKGILAFSTISQLGLIMSLLGVG
SAAFYFQDGNNALYTTAILAAVFHLINHATFKGSLFMAVGIIDHETGTRDIRNLGGLMTIMPITATVSLVGLASMAGLPP
FNGFLSKELFFTGMLNATEYGIFNLETMGYIIPIVAWIASVFTFLYCMIMFFRTFTGKFDGSKLKVDHVHEAPIGMLISP
VILGILVILFGLFPNVLAYSLIEPAMAGILPGILNPGEFFNVHITHWHGFIPELFMTIGVIVLGVILYLSMSKWQKTAFY
LGERDIFNYAYDYGYDGLIKGSQWITRIQMTGRLRDYFIYMCGFMILLIGYTMYKFNLPSFNTMELSPIEPFIWIVAIVF
IGSVIAVPFINNRLGLIILTGVTGYIVALFFVVFRAPDLALTQLLVETVTVILFMLAFYHLPKLKKEKTSKGIHAIKLII
SISFGVMITLVSLSAFSMGSTAGMPSISEYFTEFSKELAGGYNIVNVILVDFRGLDTMLEILVVGVAVLSIISLIKLRFK
GSEDV
>Mature_804_residues
TVLHWATLSPFLIAILIPFLYKYVRKVHTGWFVLLLPFTLFIYFLSFLSSTREGNVISEHLAWVPSLGINFNLYIDGLSL
LFALLITGIGTLVVLYSIFYLSDIKEKLNNFYVYLLMFMGAMLGVVLSDNLIVIYVFWEVTSIASSLLISYWYGREKSIY
GAQKSMYITVFGGLSMLGGFSLLYVIAGTFSIRELIANADIVMASSLFLPAMILVLLGAFTKSAQFPFHIWLPDAMEAPT
PVSAYLHSATMVKAGIYLVARLTGVFGGSPEWFWIITIAGLTTLAWGSISAVRQKDLKGILAFSTISQLGLIMSLLGVGS
AAFYFQDGNNALYTTAILAAVFHLINHATFKGSLFMAVGIIDHETGTRDIRNLGGLMTIMPITATVSLVGLASMAGLPPF
NGFLSKELFFTGMLNATEYGIFNLETMGYIIPIVAWIASVFTFLYCMIMFFRTFTGKFDGSKLKVDHVHEAPIGMLISPV
ILGILVILFGLFPNVLAYSLIEPAMAGILPGILNPGEFFNVHITHWHGFIPELFMTIGVIVLGVILYLSMSKWQKTAFYL
GERDIFNYAYDYGYDGLIKGSQWITRIQMTGRLRDYFIYMCGFMILLIGYTMYKFNLPSFNTMELSPIEPFIWIVAIVFI
GSVIAVPFINNRLGLIILTGVTGYIVALFFVVFRAPDLALTQLLVETVTVILFMLAFYHLPKLKKEKTSKGIHAIKLIIS
ISFGVMITLVSLSAFSMGSTAGMPSISEYFTEFSKELAGGYNIVNVILVDFRGLDTMLEILVVGVAVLSIISLIKLRFKG
SEDV

Specific function: Mnh complex is a Na(+)Li(+)/H(+) antiporter involved in Na(+) and/or Li(+) excretion and Na(+) resistance. Na(+)/H(+) antiport consumes a transmembrane electrical potential, and is thus inferred to be electrogenic. Does not transport K(+), Ca(2+) or Mg(2+

COG id: COG1009

COG function: function code CP; NADH:ubiquinone oxidoreductase subunit 5 (chain L)/Multisubunit Na+/H+ antiporter, MnhA subunit

Gene ontology:

Cell location: Cell membrane; Multi-pass membrane protein (Potential) [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the CPA3 antiporters (TC 2.A.63) subunit A family [H]

Homologues:

Organism=Homo sapiens, GI251831117, Length=443, Percent_Identity=30.2483069977427, Blast_Score=174, Evalue=3e-43,
Organism=Escherichia coli, GI1788614, Length=510, Percent_Identity=33.1372549019608, Blast_Score=213, Evalue=3e-56,
Organism=Escherichia coli, GI1788829, Length=471, Percent_Identity=33.1210191082803, Blast_Score=186, Evalue=7e-48,
Organism=Escherichia coli, GI1788831, Length=352, Percent_Identity=33.2386363636364, Blast_Score=142, Evalue=8e-35,
Organism=Escherichia coli, GI1788827, Length=447, Percent_Identity=27.0693512304251, Blast_Score=131, Evalue=2e-31,
Organism=Escherichia coli, GI2367154, Length=451, Percent_Identity=25.0554323725055, Blast_Score=110, Evalue=3e-25,
Organism=Escherichia coli, GI1788613, Length=413, Percent_Identity=26.634382566586, Blast_Score=104, Evalue=2e-23,
Organism=Escherichia coli, GI145693160, Length=342, Percent_Identity=24.2690058479532, Blast_Score=76, Evalue=8e-15,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR005663
- InterPro:   IPR001750
- InterPro:   IPR001516 [H]

Pfam domain/function: PF00361 Oxidored_q1; PF00662 Oxidored_q1_N [H]

EC number: 1.6.99.5 [C]

Molecular weight: Translated: 89263; Mature: 89132

Theoretical pI: Translated: 7.82; Mature: 7.82

Prosite motif: PS00133 CARBOXYPEPT_ZN_2

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.2 %Cys     (Translated Protein)
4.1 %Met     (Translated Protein)
4.3 %Cys+Met (Translated Protein)
0.2 %Cys     (Mature Protein)
4.0 %Met     (Mature Protein)
4.2 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MTVLHWATLSPFLIAILIPFLYKYVRKVHTGWFVLLLPFTLFIYFLSFLSSTREGNVISE
CCEEEEHHHHHHHHHHHHHHHHHHHHHHHCCHHHHHHHHHHHHHHHHHHHCCCCCCHHHH
HLAWVPSLGINFNLYIDGLSLLFALLITGIGTLVVLYSIFYLSDIKEKLNNFYVYLLMFM
HHHHCCCCCCEEEEEECHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
GAMLGVVLSDNLIVIYVFWEVTSIASSLLISYWYGREKSIYGAQKSMYITVFGGLSMLGG
HHHHHHHHCCCEEEEEEHHHHHHHHHHHHHHHHHCCCCCCCCCCCEEEEEEECCHHHHHH
FSLLYVIAGTFSIRELIANADIVMASSLFLPAMILVLLGAFTKSAQFPFHIWLPDAMEAP
HHHHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHHHCCCCCCEEEECCCCCCCC
TPVSAYLHSATMVKAGIYLVARLTGVFGGSPEWFWIITIAGLTTLAWGSISAVRQKDLKG
CCHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCEEEEEEEHHHHHHHCCHHHHHHHHHHHH
ILAFSTISQLGLIMSLLGVGSAAFYFQDGNNALYTTAILAAVFHLINHATFKGSLFMAVG
HHHHHHHHHHHHHHHHHHCCCEEEEEECCCCCHHHHHHHHHHHHHHHCCCCCCCEEEEEE
IIDHETGTRDIRNLGGLMTIMPITATVSLVGLASMAGLPPFNGFLSKELFFTGMLNATEY
EEECCCCHHHHHHCCCHHHHHHHHHHHHHHHHHHHCCCCCCCHHHHHHHHHHHCCCCHHC
GIFNLETMGYIIPIVAWIASVFTFLYCMIMFFRTFTGKFDGSKLKVDHVHEAPIGMLISP
CCEEHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCEEEECCCCCCCHHHHHHH
VILGILVILFGLFPNVLAYSLIEPAMAGILPGILNPGEFFNVHITHWHGFIPELFMTIGV
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCEEEEEEEHHHHHHHHHHHHHHH
IVLGVILYLSMSKWQKTAFYLGERDIFNYAYDYGYDGLIKGSQWITRIQMTGRLRDYFIY
HHHHHHHHHHHHHHHHHHHHCCCHHHHHHHHHCCCCCCCCCHHHHHHHHHHHHHHHHHHH
MCGFMILLIGYTMYKFNLPSFNTMELSPIEPFIWIVAIVFIGSVIAVPFINNRLGLIILT
HHHHHHHHHHHHHHEECCCCCCCEECCCCCHHHHHHHHHHHHHHHHHHHCCCCCCEEEEE
GVTGYIVALFFVVFRAPDLALTQLLVETVTVILFMLAFYHLPKLKKEKTSKGIHAIKLII
CHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHHHHHCCCHHHHHHHCCHHHHHHHH
SISFGVMITLVSLSAFSMGSTAGMPSISEYFTEFSKELAGGYNIVNVILVDFRGLDTMLE
HHHHHHHHHHHHHHHHCCCCCCCCCCHHHHHHHHHHHHCCCCCEEHHHEEHHCCHHHHHH
ILVVGVAVLSIISLIKLRFKGSEDV
HHHHHHHHHHHHHHHHHHCCCCCCC
>Mature Secondary Structure 
TVLHWATLSPFLIAILIPFLYKYVRKVHTGWFVLLLPFTLFIYFLSFLSSTREGNVISE
CEEEEHHHHHHHHHHHHHHHHHHHHHHHCCHHHHHHHHHHHHHHHHHHHCCCCCCHHHH
HLAWVPSLGINFNLYIDGLSLLFALLITGIGTLVVLYSIFYLSDIKEKLNNFYVYLLMFM
HHHHCCCCCCEEEEEECHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
GAMLGVVLSDNLIVIYVFWEVTSIASSLLISYWYGREKSIYGAQKSMYITVFGGLSMLGG
HHHHHHHHCCCEEEEEEHHHHHHHHHHHHHHHHHCCCCCCCCCCCEEEEEEECCHHHHHH
FSLLYVIAGTFSIRELIANADIVMASSLFLPAMILVLLGAFTKSAQFPFHIWLPDAMEAP
HHHHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHHHCCCCCCEEEECCCCCCCC
TPVSAYLHSATMVKAGIYLVARLTGVFGGSPEWFWIITIAGLTTLAWGSISAVRQKDLKG
CCHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCEEEEEEEHHHHHHHCCHHHHHHHHHHHH
ILAFSTISQLGLIMSLLGVGSAAFYFQDGNNALYTTAILAAVFHLINHATFKGSLFMAVG
HHHHHHHHHHHHHHHHHHCCCEEEEEECCCCCHHHHHHHHHHHHHHHCCCCCCCEEEEEE
IIDHETGTRDIRNLGGLMTIMPITATVSLVGLASMAGLPPFNGFLSKELFFTGMLNATEY
EEECCCCHHHHHHCCCHHHHHHHHHHHHHHHHHHHCCCCCCCHHHHHHHHHHHCCCCHHC
GIFNLETMGYIIPIVAWIASVFTFLYCMIMFFRTFTGKFDGSKLKVDHVHEAPIGMLISP
CCEEHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCEEEECCCCCCCHHHHHHH
VILGILVILFGLFPNVLAYSLIEPAMAGILPGILNPGEFFNVHITHWHGFIPELFMTIGV
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCEEEEEEEHHHHHHHHHHHHHHH
IVLGVILYLSMSKWQKTAFYLGERDIFNYAYDYGYDGLIKGSQWITRIQMTGRLRDYFIY
HHHHHHHHHHHHHHHHHHHHCCCHHHHHHHHHCCCCCCCCCHHHHHHHHHHHHHHHHHHH
MCGFMILLIGYTMYKFNLPSFNTMELSPIEPFIWIVAIVFIGSVIAVPFINNRLGLIILT
HHHHHHHHHHHHHHEECCCCCCCEECCCCCHHHHHHHHHHHHHHHHHHHCCCCCCEEEEE
GVTGYIVALFFVVFRAPDLALTQLLVETVTVILFMLAFYHLPKLKKEKTSKGIHAIKLII
CHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHHHHHCCCHHHHHHHCCHHHHHHHH
SISFGVMITLVSLSAFSMGSTAGMPSISEYFTEFSKELAGGYNIVNVILVDFRGLDTMLE
HHHHHHHHHHHHHHHHCCCCCCCCCCHHHHHHHHHHHHCCCCCEEHHHEEHHCCHHHHHH
ILVVGVAVLSIISLIKLRFKGSEDV
HHHHHHHHHHHHHHHHHHCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: Iron [C]

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NADH; ubiquinone [C]

Specific reaction: NADH + ubiquinone = NAD+ + ubiquinol [C]

General reaction: Redox reaction [C]

Inhibitor: NA

Structure determination priority: 6.0

TargetDB status: NA

Availability: NA

References: 11356194 [H]