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The map label for this gene is grpE

Identifier: 23099424

GI number: 23099424

Start: 1997237

End: 1997809

Strand: Reverse

Name: grpE

Synonym: OB1969

Alternate gene names: 23099424

Gene position: 1997809-1997237 (Counterclockwise)

Preceding gene: 23099425

Following gene: 23099423

Centisome position: 55.03

GC content: 34.38

Gene sequence:

>573_bases
TTGAACGAAAAAGATAATCAAACAACTTCTGAACCAGAAAATGAACAAGAAATAATTGATGTAAATGATTCTGGTGAACA
GCCAGAGGAGAATGAAACAGAACAACCTCAAGAAGAAGCTGTTGAAAATGATGAAATAGCTAAATTACAACAAGAAAAAG
ATGAAACTTACAATCGTTTAGTACGTCTTCAAGCAGAATTTGATAATTACAAACGAAGAACGCTAAAAGAAAGAGAAGCG
GATCGTAAGTATAAATCACAAGATCTTATTACAGAACTTTTACCAGCTATTGATAACTTTGAACGAGCTTTACAGGTTGA
AGTTACAGAAGAAAACAAAAGCATTATTGATGGAATAATGATGGTGTATCGCCAACTACAAGAAGCTCTAACATCTCAGG
GTGTTGAACCAATTAAAACGGAGGGTGAGGTATTTGACCCGAATCTTCATCACGCTGTAATGCAAATTGAAGATGAAAAT
ATGGATTCCAATACAGTTGTAGAGGAATTACAAAAAGGATACCAGCTTAAAGATCGTGTTATTCGCCCTGCGATGGTAAA
AGTGAATAAATAA

Upstream 100 bases:

>100_bases
AGATTCTTAACTTAAGTTCTTAATAGAACGTCTTGGTTTTCTAAATAGTGCAATCATGATATAGTCAATGATGGAAATTG
AAAGCGAGGTGCCACTAAAA

Downstream 100 bases:

>100_bases
TCGAAAGTTATTGCAGTTAAGGAGGAAACAAACAATGAGTAAAATTATTGGAATTGACTTAGGAACTACAAATTCATGTG
TATCAGTAATGGAAGGCGGC

Product: heat shock protein

Products: NA

Alternate protein names: HSP-70 cofactor

Number of amino acids: Translated: 190; Mature: 190

Protein sequence:

>190_residues
MNEKDNQTTSEPENEQEIIDVNDSGEQPEENETEQPQEEAVENDEIAKLQQEKDETYNRLVRLQAEFDNYKRRTLKEREA
DRKYKSQDLITELLPAIDNFERALQVEVTEENKSIIDGIMMVYRQLQEALTSQGVEPIKTEGEVFDPNLHHAVMQIEDEN
MDSNTVVEELQKGYQLKDRVIRPAMVKVNK

Sequences:

>Translated_190_residues
MNEKDNQTTSEPENEQEIIDVNDSGEQPEENETEQPQEEAVENDEIAKLQQEKDETYNRLVRLQAEFDNYKRRTLKEREA
DRKYKSQDLITELLPAIDNFERALQVEVTEENKSIIDGIMMVYRQLQEALTSQGVEPIKTEGEVFDPNLHHAVMQIEDEN
MDSNTVVEELQKGYQLKDRVIRPAMVKVNK
>Mature_190_residues
MNEKDNQTTSEPENEQEIIDVNDSGEQPEENETEQPQEEAVENDEIAKLQQEKDETYNRLVRLQAEFDNYKRRTLKEREA
DRKYKSQDLITELLPAIDNFERALQVEVTEENKSIIDGIMMVYRQLQEALTSQGVEPIKTEGEVFDPNLHHAVMQIEDEN
MDSNTVVEELQKGYQLKDRVIRPAMVKVNK

Specific function: Participates actively in the response to hyperosmotic and heat shock by preventing the aggregation of stress-denatured proteins, in association with dnaK and grpE. It is the nucleotide exchange factor for dnaK and may function as a thermosensor. Unfolded

COG id: COG0576

COG function: function code O; Molecular chaperone GrpE (heat shock protein)

Gene ontology:

Cell location: Cytoplasm (Probable)

Metaboloic importance: Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the grpE family

Homologues:

Organism=Homo sapiens, GI24308295, Length=168, Percent_Identity=29.7619047619048, Blast_Score=70, Evalue=8e-13,
Organism=Escherichia coli, GI1788967, Length=195, Percent_Identity=32.8205128205128, Blast_Score=93, Evalue=9e-21,
Organism=Saccharomyces cerevisiae, GI6324806, Length=162, Percent_Identity=33.3333333333333, Blast_Score=77, Evalue=1e-15,
Organism=Drosophila melanogaster, GI24653432, Length=175, Percent_Identity=29.7142857142857, Blast_Score=78, Evalue=4e-15,

Paralogues:

None

Copy number: 2359 Molecules/Cell In: Growth Phase, Glucose-minimal MOPS Media. 4,000 Molecules/Cell In: Glucose minimal media [C]

Swissprot (AC and ID): GRPE_OCEIH (Q8CXD2)

Other databases:

- EMBL:   BA000028
- RefSeq:   NP_692890.1
- ProteinModelPortal:   Q8CXD2
- SMR:   Q8CXD2
- GeneID:   1018446
- GenomeReviews:   BA000028_GR
- KEGG:   oih:OB1969
- NMPDR:   fig|221109.1.peg.1969
- HOGENOM:   HBG732630
- OMA:   NLHQAVM
- BioCyc:   OIHE221109:OB1969-MONOMER
- GO:   GO:0005739
- HAMAP:   MF_01151
- InterPro:   IPR000740
- InterPro:   IPR013805
- InterPro:   IPR009012
- Gene3D:   G3DSA:3.90.20.20
- Gene3D:   G3DSA:2.30.22.10
- PANTHER:   PTHR21237
- PRINTS:   PR00773

Pfam domain/function: PF01025 GrpE; SSF51064 GrpE_head

EC number: NA

Molecular weight: Translated: 22198; Mature: 22198

Theoretical pI: Translated: 4.12; Mature: 4.12

Prosite motif: PS01071 GRPE

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.0 %Cys     (Translated Protein)
3.2 %Met     (Translated Protein)
3.2 %Cys+Met (Translated Protein)
0.0 %Cys     (Mature Protein)
3.2 %Met     (Mature Protein)
3.2 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MNEKDNQTTSEPENEQEIIDVNDSGEQPEENETEQPQEEAVENDEIAKLQQEKDETYNRL
CCCCCCCCCCCCCCCCCEEECCCCCCCCCCCCCCCCHHHHHCCHHHHHHHHHHHHHHHHH
VRLQAEFDNYKRRTLKEREADRKYKSQDLITELLPAIDNFERALQVEVTEENKSIIDGIM
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHEEEEECCCHHHHHHHHH
MVYRQLQEALTSQGVEPIKTEGEVFDPNLHHAVMQIEDENMDSNTVVEELQKGYQLKDRV
HHHHHHHHHHHHCCCCCCCCCCCEECCCHHHHHHEECCCCCCHHHHHHHHHHCHHHHHHH
IRPAMVKVNK
HCCHHHEECC
>Mature Secondary Structure
MNEKDNQTTSEPENEQEIIDVNDSGEQPEENETEQPQEEAVENDEIAKLQQEKDETYNRL
CCCCCCCCCCCCCCCCCEEECCCCCCCCCCCCCCCCHHHHHCCHHHHHHHHHHHHHHHHH
VRLQAEFDNYKRRTLKEREADRKYKSQDLITELLPAIDNFERALQVEVTEENKSIIDGIM
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHEEEEECCCHHHHHHHHH
MVYRQLQEALTSQGVEPIKTEGEVFDPNLHHAVMQIEDENMDSNTVVEELQKGYQLKDRV
HHHHHHHHHHHHCCCCCCCCCCCEECCCHHHHHHEECCCCCCHHHHHHHHHHCHHHHHHH
IRPAMVKVNK
HCCHHHEECC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 12235376