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The map label for this gene is nfo

Identifier: 23099393

GI number: 23099393

Start: 1964687

End: 1965580

Strand: Reverse

Name: nfo

Synonym: OB1938

Alternate gene names: 23099393

Gene position: 1965580-1964687 (Counterclockwise)

Preceding gene: 23099394

Following gene: 23099391

Centisome position: 54.14

GC content: 35.01

Gene sequence:

>894_bases
ATGGTAAAGATTGGATCACATGTTTCAATGAATGGCAAGAAAATGCTATTAGGCTCCAGTGAAGATGCTGTTCAATATGG
AGCTAATACGTTTATGATCTACACAGGTGCCCCTCAAAATACAAGAAGGAAGCCTATAGAAGAGCTGAATATTGAAGCAG
GTACAGAACATATGAAAGCCAATGGAATCCAAGATATAGTAGTACATGCACCGTATATTATAAATATTGGTAATTCCATT
AAACCAGCTACTTTTGAGTTGGGAGTTAACTTTTTAAAAAATGAAATTGATCGTACAGAGGCATTAGGTGCGAAACAAAT
TGTACTTCATCCAGGAGCGCATGTTGGGGAAGGTGCTGAAAAAGGAATACCAAAAATTATAGAAGGTTTAAATGAAGTTC
TAGATCCAAATAGTAATGTCCAAATTGCTTTGGAAACAATGGCAGGAAAAGGATCTGAAATAGGACGTACATTTGAAGAG
CTAGCTCAAATTATCGAAGGTGTAACACACAATGATCGGCTGTCTATTTGTATGGATACATGCCATATTCACGATGCAGG
CTACAACATTGTAGAAGATTTTGATGGAGTGTTAGAGCAATTTGATAAAATCATTGGGATAGACCGATTAAAAGTTGTCC
ATGTAAATGATAGTAAAAATGAGCGAGGTGCTCATAAAGATCGTCATGAAAATATTGGTTTTGGTTATATCGGCTTTGAA
GCTCTGCATAATATAGTGCACCATCCACAATTATCTGATCTACCTAAAATTTTGGAAACACCTTTTGTAGGTACAGACAA
GAAAAACAAAAAACCTCCTTATAAACATGAAATAGAAATGTTAAAAGAAGGCAATTTTGATCCACATCTAAAAGAAAAAA
TAATGGAAGCATAA

Upstream 100 bases:

>100_bases
AACCAGGATATAAGAAGAAACAAAAACGCCAACAACAAGAAATTAAAAAACAGTTAACTAAAAAAAAGAAAAAGTAAAGT
AAGCGAAGAGGAGGAGTAAA

Downstream 100 bases:

>100_bases
CTGAGTGAAGCTGATTATAACTTTATTTTATAATCAGCTTCTTTTTTAGTTAAAGAGATGTCCTAATCCGTAAGATTGAA
TAACTTCATCCATTAAAGCT

Product: endonuclease IV

Products: NA

Alternate protein names: Endodeoxyribonuclease IV; Endonuclease IV

Number of amino acids: Translated: 297; Mature: 297

Protein sequence:

>297_residues
MVKIGSHVSMNGKKMLLGSSEDAVQYGANTFMIYTGAPQNTRRKPIEELNIEAGTEHMKANGIQDIVVHAPYIINIGNSI
KPATFELGVNFLKNEIDRTEALGAKQIVLHPGAHVGEGAEKGIPKIIEGLNEVLDPNSNVQIALETMAGKGSEIGRTFEE
LAQIIEGVTHNDRLSICMDTCHIHDAGYNIVEDFDGVLEQFDKIIGIDRLKVVHVNDSKNERGAHKDRHENIGFGYIGFE
ALHNIVHHPQLSDLPKILETPFVGTDKKNKKPPYKHEIEMLKEGNFDPHLKEKIMEA

Sequences:

>Translated_297_residues
MVKIGSHVSMNGKKMLLGSSEDAVQYGANTFMIYTGAPQNTRRKPIEELNIEAGTEHMKANGIQDIVVHAPYIINIGNSI
KPATFELGVNFLKNEIDRTEALGAKQIVLHPGAHVGEGAEKGIPKIIEGLNEVLDPNSNVQIALETMAGKGSEIGRTFEE
LAQIIEGVTHNDRLSICMDTCHIHDAGYNIVEDFDGVLEQFDKIIGIDRLKVVHVNDSKNERGAHKDRHENIGFGYIGFE
ALHNIVHHPQLSDLPKILETPFVGTDKKNKKPPYKHEIEMLKEGNFDPHLKEKIMEA
>Mature_297_residues
MVKIGSHVSMNGKKMLLGSSEDAVQYGANTFMIYTGAPQNTRRKPIEELNIEAGTEHMKANGIQDIVVHAPYIINIGNSI
KPATFELGVNFLKNEIDRTEALGAKQIVLHPGAHVGEGAEKGIPKIIEGLNEVLDPNSNVQIALETMAGKGSEIGRTFEE
LAQIIEGVTHNDRLSICMDTCHIHDAGYNIVEDFDGVLEQFDKIIGIDRLKVVHVNDSKNERGAHKDRHENIGFGYIGFE
ALHNIVHHPQLSDLPKILETPFVGTDKKNKKPPYKHEIEMLKEGNFDPHLKEKIMEA

Specific function: Endonuclease IV plays a role in DNA repair. It cleaves phosphodiester bonds at apurinic or apyrimidinic sites (AP sites) to produce new 5'-ends that are base-free deoxyribose 5-phosphate residues. It preferentially attacks modified AP sites created by ble

COG id: COG0648

COG function: function code L; Endonuclease IV

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the AP endonuclease 2 family

Homologues:

Organism=Escherichia coli, GI1788483, Length=266, Percent_Identity=32.7067669172932, Blast_Score=141, Evalue=4e-35,
Organism=Caenorhabditis elegans, GI17531193, Length=263, Percent_Identity=34.6007604562738, Blast_Score=147, Evalue=6e-36,
Organism=Saccharomyces cerevisiae, GI6322735, Length=267, Percent_Identity=30.3370786516854, Blast_Score=143, Evalue=3e-35,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): END4_OCEIH (Q8EPZ2)

Other databases:

- EMBL:   BA000028
- RefSeq:   NP_692859.1
- ProteinModelPortal:   Q8EPZ2
- SMR:   Q8EPZ2
- GeneID:   1018418
- GenomeReviews:   BA000028_GR
- KEGG:   oih:OB1938
- NMPDR:   fig|221109.1.peg.1938
- HOGENOM:   HBG565018
- OMA:   QIALETM
- ProtClustDB:   PRK01060
- BioCyc:   OIHE221109:OB1938-MONOMER
- BRENDA:   3.1.21.2
- GO:   GO:0005622
- HAMAP:   MF_00152
- InterPro:   IPR018246
- InterPro:   IPR001719
- InterPro:   IPR013022
- InterPro:   IPR012307
- Gene3D:   G3DSA:3.20.20.150
- PANTHER:   PTHR21445
- SMART:   SM00518
- TIGRFAMs:   TIGR00587

Pfam domain/function: PF01261 AP_endonuc_2; SSF51658 Xyl_isomerase-like_TIM-brl

EC number: =3.1.21.2

Molecular weight: Translated: 32919; Mature: 32919

Theoretical pI: Translated: 6.08; Mature: 6.08

Prosite motif: PS00729 AP_NUCLEASE_F2_1; PS00730 AP_NUCLEASE_F2_2; PS00731 AP_NUCLEASE_F2_3; PS51432 AP_NUCLEASE_F2_4

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.7 %Cys     (Translated Protein)
3.0 %Met     (Translated Protein)
3.7 %Cys+Met (Translated Protein)
0.7 %Cys     (Mature Protein)
3.0 %Met     (Mature Protein)
3.7 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MVKIGSHVSMNGKKMLLGSSEDAVQYGANTFMIYTGAPQNTRRKPIEELNIEAGTEHMKA
CEEECCEECCCCCEEEECCCHHHHHHCCCEEEEEECCCCCCCCCCHHHCCCCCCCHHHHH
NGIQDIVVHAPYIINIGNSIKPATFELGVNFLKNEIDRTEALGAKQIVLHPGAHVGEGAE
CCCHHHEEECCEEEECCCCCCCHHHHHHHHHHHHHHHHHHHCCCCEEEECCCCCCCCCHH
KGIPKIIEGLNEVLDPNSNVQIALETMAGKGSEIGRTFEELAQIIEGVTHNDRLSICMDT
CCCHHHHHHHHHHHCCCCCEEEEEEECCCCCHHHHHHHHHHHHHHHCCCCCCCEEEEEHH
CHIHDAGYNIVEDFDGVLEQFDKIIGIDRLKVVHVNDSKNERGAHKDRHENIGFGYIGFE
HHHCCCCCHHHHHHHHHHHHHHHHHCCCCEEEEEECCCCCCCCCCCHHHCCCCCCCHHHH
ALHNIVHHPQLSDLPKILETPFVGTDKKNKKPPYKHEIEMLKEGNFDPHLKEKIMEA
HHHHHHCCCCHHHHHHHHHCCCCCCCCCCCCCCHHHHHHHHHCCCCCHHHHHHHHCC
>Mature Secondary Structure
MVKIGSHVSMNGKKMLLGSSEDAVQYGANTFMIYTGAPQNTRRKPIEELNIEAGTEHMKA
CEEECCEECCCCCEEEECCCHHHHHHCCCEEEEEECCCCCCCCCCHHHCCCCCCCHHHHH
NGIQDIVVHAPYIINIGNSIKPATFELGVNFLKNEIDRTEALGAKQIVLHPGAHVGEGAE
CCCHHHEEECCEEEECCCCCCCHHHHHHHHHHHHHHHHHHHCCCCEEEECCCCCCCCCHH
KGIPKIIEGLNEVLDPNSNVQIALETMAGKGSEIGRTFEELAQIIEGVTHNDRLSICMDT
CCCHHHHHHHHHHHCCCCCEEEEEEECCCCCHHHHHHHHHHHHHHHCCCCCCCEEEEEHH
CHIHDAGYNIVEDFDGVLEQFDKIIGIDRLKVVHVNDSKNERGAHKDRHENIGFGYIGFE
HHHCCCCCHHHHHHHHHHHHHHHHHCCCCEEEEEECCCCCCCCCCCHHHCCCCCCCHHHH
ALHNIVHHPQLSDLPKILETPFVGTDKKNKKPPYKHEIEMLKEGNFDPHLKEKIMEA
HHHHHHCCCCHHHHHHHHHCCCCCCCCCCCCCCHHHHHHHHHCCCCCHHHHHHHHCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 12235376