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The map label for this gene is recN [H]

Identifier: 23099329

GI number: 23099329

Start: 1908613

End: 1910307

Strand: Reverse

Name: recN [H]

Synonym: OB1874

Alternate gene names: 23099329

Gene position: 1910307-1908613 (Counterclockwise)

Preceding gene: 23099330

Following gene: 23099328

Centisome position: 52.62

GC content: 33.92

Gene sequence:

>1695_bases
GTGTTAACCGAATTATCAATTCAAGATTTTGCTATTATCGATGACATATCTATTACTTTTAATGACGGACTAACGGTTTT
AACAGGTGAAACGGGAGCTGGAAAATCCATTATAATTGATGCAATTCAATTATTAGCAGGTGGAAGAGGTTCTGTAGACT
ATGTCAGACATGGTACAAAAAAAGCAACAATTGAAGGATTGTTTTTTATTTCGAACAACAATCATCCTATATATCATACA
GGTAATGAATTTGGAATAGAGATACAAGACGAACAAGTTGTATTACAACGGATCATTACTTCTTCTGGTAAAAGTATTTG
CCGCGTTAATGGTAAATTAGTTACTTTAGCTATATTACGTGAATTTGGTAAAACATTAATTGATATTCATAGTCAACACG
AAACACAGTCATTAATGGATGTTGACCATCATATTGACTTTCTTGATATGTATGACTACAAGGCGATCGAAGAAACAAAG
TCTGAATACAGAAAAATATATGATGCACTTGAAACAGTAAAACGTAAGTATCGTAAGTTAAATGATAATGAGCAAGAGAT
GGCACAACGACTTGATTTGTTACAATTTCAACAGCAGGAATTGGAACAAGCGCAATTACAGCCTGAAGAAGACATTGAAA
TAGATGAAGAAATTCATCAGCTTGCTAATTATGAAAAATTACATCGAGCATTAAATGATGCTTATTACGCTCTTTATGGG
GAACAACGGGGCCTTGATTTTGTGAAACAAGCGCAACTAGCATTGGAAACTAATCAAGATGTTGATCCGTTTATTACTGA
AAAAAATGAAGAAATCGCAAACCATTTTTATGCTTTAGAAGAGTTGGCATACTCATTACGGAACCATTTAGATGGCCTAC
AATATAACCCTGAACGATTAAATGAATTAGAGCAAAGAAAAGATGAATTAAATAAGTTAAAACGAAAATATGGTACCACA
GTGGAAGAAATGTTATCTTATTTAGCTAAAATTGATGAAGAAATAGAGCAAATAACGAATCGTGATACACATGTTAATGC
ATTAGTTGCGGAAATAGAAGAACTCGAAAAAGATGCTATGTTAGAAGCAGAACAACTCCATCATTTACGCGCTCTTGCAG
CAGAAAACTTGGTGAATGAAATACATCAAGAGCTCAAAGATTTATACTTAGAAAATGCATCATTTGCTATTGACTTTGAA
GATGCAACACCACCAAAGTTAACAAAAAATGGATATGATAAAGTTCGTTTTTTGATTTCAACGAATGTTGGCGAACCATT
GAAAGATTTATCAAAAGTAGCATCAGGTGGAGAATTATCAAGATTTATGCTTGCACTTAAAAAAATATTTTCCAAACATC
AGGGTATTACCAGTGTGATTTTCGACGAAGTGGATACTGGAGTGAGTGGACGTGTCGCACAAGCTATTGCTGAAAAAATG
CATCAAGTCTCTACAGATTCTCAAGTACTTTGTATTACTCACCTTCCCCAAGTAGCAGCAATGGCAGATACTCATAAGTT
TATTCGGAAAAATGAGAAGTCAAATCGCACATACACAAGCGTTCAAGAATTAAATGAGGAAGAACAGATTGATGAATTAA
GTCGCATGATTACAGGTACGAAACTTACCGACACTGCAAAAAGTCATGCAAGAGAAATGCTAGAGCTTGCATCAAACTTT
AAAGTAAAAAATTAA

Upstream 100 bases:

>100_bases
TACATGTTTAATCATTTGCAGAACTCCAGAACAAGCAGAAATGATTAAAAATCGCTTTATTGAAATGTTATAAGTTGGAA
ACAATGGGGTGGGTAGAAAA

Downstream 100 bases:

>100_bases
AATAAATAGGATAAAATTATTTAAAAACCGGAAAATATACTTACCGGTTTTTTTATTTTACCTCGATTAACAAATATAAC
TAAACTCCTTACATGCATAT

Product: DNA repair and genetic recombination

Products: NA

Alternate protein names: Recombination protein N [H]

Number of amino acids: Translated: 564; Mature: 564

Protein sequence:

>564_residues
MLTELSIQDFAIIDDISITFNDGLTVLTGETGAGKSIIIDAIQLLAGGRGSVDYVRHGTKKATIEGLFFISNNNHPIYHT
GNEFGIEIQDEQVVLQRIITSSGKSICRVNGKLVTLAILREFGKTLIDIHSQHETQSLMDVDHHIDFLDMYDYKAIEETK
SEYRKIYDALETVKRKYRKLNDNEQEMAQRLDLLQFQQQELEQAQLQPEEDIEIDEEIHQLANYEKLHRALNDAYYALYG
EQRGLDFVKQAQLALETNQDVDPFITEKNEEIANHFYALEELAYSLRNHLDGLQYNPERLNELEQRKDELNKLKRKYGTT
VEEMLSYLAKIDEEIEQITNRDTHVNALVAEIEELEKDAMLEAEQLHHLRALAAENLVNEIHQELKDLYLENASFAIDFE
DATPPKLTKNGYDKVRFLISTNVGEPLKDLSKVASGGELSRFMLALKKIFSKHQGITSVIFDEVDTGVSGRVAQAIAEKM
HQVSTDSQVLCITHLPQVAAMADTHKFIRKNEKSNRTYTSVQELNEEEQIDELSRMITGTKLTDTAKSHAREMLELASNF
KVKN

Sequences:

>Translated_564_residues
MLTELSIQDFAIIDDISITFNDGLTVLTGETGAGKSIIIDAIQLLAGGRGSVDYVRHGTKKATIEGLFFISNNNHPIYHT
GNEFGIEIQDEQVVLQRIITSSGKSICRVNGKLVTLAILREFGKTLIDIHSQHETQSLMDVDHHIDFLDMYDYKAIEETK
SEYRKIYDALETVKRKYRKLNDNEQEMAQRLDLLQFQQQELEQAQLQPEEDIEIDEEIHQLANYEKLHRALNDAYYALYG
EQRGLDFVKQAQLALETNQDVDPFITEKNEEIANHFYALEELAYSLRNHLDGLQYNPERLNELEQRKDELNKLKRKYGTT
VEEMLSYLAKIDEEIEQITNRDTHVNALVAEIEELEKDAMLEAEQLHHLRALAAENLVNEIHQELKDLYLENASFAIDFE
DATPPKLTKNGYDKVRFLISTNVGEPLKDLSKVASGGELSRFMLALKKIFSKHQGITSVIFDEVDTGVSGRVAQAIAEKM
HQVSTDSQVLCITHLPQVAAMADTHKFIRKNEKSNRTYTSVQELNEEEQIDELSRMITGTKLTDTAKSHAREMLELASNF
KVKN
>Mature_564_residues
MLTELSIQDFAIIDDISITFNDGLTVLTGETGAGKSIIIDAIQLLAGGRGSVDYVRHGTKKATIEGLFFISNNNHPIYHT
GNEFGIEIQDEQVVLQRIITSSGKSICRVNGKLVTLAILREFGKTLIDIHSQHETQSLMDVDHHIDFLDMYDYKAIEETK
SEYRKIYDALETVKRKYRKLNDNEQEMAQRLDLLQFQQQELEQAQLQPEEDIEIDEEIHQLANYEKLHRALNDAYYALYG
EQRGLDFVKQAQLALETNQDVDPFITEKNEEIANHFYALEELAYSLRNHLDGLQYNPERLNELEQRKDELNKLKRKYGTT
VEEMLSYLAKIDEEIEQITNRDTHVNALVAEIEELEKDAMLEAEQLHHLRALAAENLVNEIHQELKDLYLENASFAIDFE
DATPPKLTKNGYDKVRFLISTNVGEPLKDLSKVASGGELSRFMLALKKIFSKHQGITSVIFDEVDTGVSGRVAQAIAEKM
HQVSTDSQVLCITHLPQVAAMADTHKFIRKNEKSNRTYTSVQELNEEEQIDELSRMITGTKLTDTAKSHAREMLELASNF
KVKN

Specific function: Involved in recombinational repair of damaged DNA. Seems to be the first protein recruited to repair centers, foci that are the site of double-strand DNA break(s), followed by recO and then recF [H]

COG id: COG0497

COG function: function code L; ATPase involved in DNA repair

Gene ontology:

Cell location: Cytoplasm, nucleoid. Note=Cytoplasmically located in untreated cells. Recruited to foci following treatment with DNA damaging agents; these foci are presumably the breaks themselves. They are almost always located within nucleoids [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the recN family [H]

Homologues:

Organism=Escherichia coli, GI48994901, Length=566, Percent_Identity=34.0989399293286, Blast_Score=287, Evalue=1e-78,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR004604
- InterPro:   IPR003395 [H]

Pfam domain/function: PF02463 SMC_N [H]

EC number: NA

Molecular weight: Translated: 64435; Mature: 64435

Theoretical pI: Translated: 4.71; Mature: 4.71

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.4 %Cys     (Translated Protein)
2.0 %Met     (Translated Protein)
2.3 %Cys+Met (Translated Protein)
0.4 %Cys     (Mature Protein)
2.0 %Met     (Mature Protein)
2.3 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MLTELSIQDFAIIDDISITFNDGLTVLTGETGAGKSIIIDAIQLLAGGRGSVDYVRHGTK
CCCCCCCCCEEEEECCEEEECCCEEEEECCCCCCHHHHHHHHHHHHCCCCCHHHHHCCCC
KATIEGLFFISNNNHPIYHTGNEFGIEIQDEQVVLQRIITSSGKSICRVNGKLVTLAILR
CEEEEEEEEEECCCCCEEECCCCCCEEECHHHHHHHHHHHHCCCCEEEECCCHHHHHHHH
EFGKTLIDIHSQHETQSLMDVDHHIDFLDMYDYKAIEETKSEYRKIYDALETVKRKYRKL
HHHHHHHHHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHC
NDNEQEMAQRLDLLQFQQQELEQAQLQPEEDIEIDEEIHQLANYEKLHRALNDAYYALYG
CCCHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHEEC
EQRGLDFVKQAQLALETNQDVDPFITEKNEEIANHFYALEELAYSLRNHLDGLQYNPERL
CCCCHHHHHHHHHHHHCCCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHCCCCCCCHHHH
NELEQRKDELNKLKRKYGTTVEEMLSYLAKIDEEIEQITNRDTHVNALVAEIEELEKDAM
HHHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHH
LEAEQLHHLRALAAENLVNEIHQELKDLYLENASFAIDFEDATPPKLTKNGYDKVRFLIS
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCEEEEECCCCCCCCCCCCHHHEEEEEE
TNVGEPLKDLSKVASGGELSRFMLALKKIFSKHQGITSVIFDEVDTGVSGRVAQAIAEKM
CCCCCHHHHHHHHHCCCHHHHHHHHHHHHHHHHCCHHHHHHHHHCCCCCHHHHHHHHHHH
HQVSTDSQVLCITHLPQVAAMADTHKFIRKNEKSNRTYTSVQELNEEEQIDELSRMITGT
HHCCCCCCEEEEECCHHHHHHHHHHHHHHCCCCCCCHHHHHHHCCHHHHHHHHHHHHCCC
KLTDTAKSHAREMLELASNFKVKN
CHHHHHHHHHHHHHHHHHCCCCCC
>Mature Secondary Structure
MLTELSIQDFAIIDDISITFNDGLTVLTGETGAGKSIIIDAIQLLAGGRGSVDYVRHGTK
CCCCCCCCCEEEEECCEEEECCCEEEEECCCCCCHHHHHHHHHHHHCCCCCHHHHHCCCC
KATIEGLFFISNNNHPIYHTGNEFGIEIQDEQVVLQRIITSSGKSICRVNGKLVTLAILR
CEEEEEEEEEECCCCCEEECCCCCCEEECHHHHHHHHHHHHCCCCEEEECCCHHHHHHHH
EFGKTLIDIHSQHETQSLMDVDHHIDFLDMYDYKAIEETKSEYRKIYDALETVKRKYRKL
HHHHHHHHHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHC
NDNEQEMAQRLDLLQFQQQELEQAQLQPEEDIEIDEEIHQLANYEKLHRALNDAYYALYG
CCCHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHEEC
EQRGLDFVKQAQLALETNQDVDPFITEKNEEIANHFYALEELAYSLRNHLDGLQYNPERL
CCCCHHHHHHHHHHHHCCCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHCCCCCCCHHHH
NELEQRKDELNKLKRKYGTTVEEMLSYLAKIDEEIEQITNRDTHVNALVAEIEELEKDAM
HHHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHH
LEAEQLHHLRALAAENLVNEIHQELKDLYLENASFAIDFEDATPPKLTKNGYDKVRFLIS
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCEEEEECCCCCCCCCCCCHHHEEEEEE
TNVGEPLKDLSKVASGGELSRFMLALKKIFSKHQGITSVIFDEVDTGVSGRVAQAIAEKM
CCCCCHHHHHHHHHCCCHHHHHHHHHHHHHHHHCCHHHHHHHHHCCCCCHHHHHHHHHHH
HQVSTDSQVLCITHLPQVAAMADTHKFIRKNEKSNRTYTSVQELNEEEQIDELSRMITGT
HHCCCCCCEEEEECCHHHHHHHHHHHHHHCCCCCCCHHHHHHHCCHHHHHHHHHHHHCCC
KLTDTAKSHAREMLELASNFKVKN
CHHHHHHHHHHHHHHHHHCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 2106508; 8969508; 9384377; 2507400; 9044256 [H]