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The map label for this gene is yclM [H]

Identifier: 23099326

GI number: 23099326

Start: 1904419

End: 1905813

Strand: Direct

Name: yclM [H]

Synonym: OB1871

Alternate gene names: 23099326

Gene position: 1904419-1905813 (Clockwise)

Preceding gene: 28563933

Following gene: 23099353

Centisome position: 52.46

GC content: 34.98

Gene sequence:

>1395_bases
ATGAAAGTAGCAAAATTTGGAGGAAGCTCAGTTGCAAATGCAGAACAAATTCAGAAAGTAGCAAATATTATCAAGGCGGA
TCCCTCCAGAAAAGTTGTTGTTGTATCCGCACCCGGTAAAAGATTTAAAGACGATGTCAAAATCACTGATCTATTAATTG
AATTTGCTGGCAGTCATCAGCGAAAACAATTCGAACAAAGTGAGAAAACATTAACTATTATAATGGACCGTTTCAAGGAA
ATTGTCGACAATTTAAATTTGCATACCAGTATAATTGATGATATTAAACAAACAATTGAAGAAGATTTAAATAAGGATCT
ATCACCAAACCAACTTTTAGATGCAATTAAAGCAATTGGTGAAGATAGCTCGGCAAAAATAGTTAGTGCATACCTTCAAC
ATATAGGTATAGATGCTACATATATGAACCCGAAGGATGCAGGTATATATGTTAGTAATGAACCAGGACATGCACAATTA
TTACCTGAAAGTTTTCCGATTATTAAAAAGCTACGTGATATTGATAAAGTCGTAGTCATCCCAGGATTCTTTGGATATGC
CGAGAATGGAGAACTTGTTACATTCTCTAGAGGCGGTTCCGATATTACTGGTTCGATCTTAGCTGCAGGGACAAAGGCAG
AATTATATGAGAATTTCACTGATGTAGATTCGGTATATGCTGTTAACCCAACAATTGTTAATAATCCGAAAGAAATAAAT
GTATTGACGTATAAAGAAATGCGAGAATTATCATACGCTGGTTTCTCTGTTTTTCATGATGAAGCATTAATTCCTGCTTT
TCGTGAACAAATTCCCGTATGTATTAAGAACACCAATAATCCAGATTCTCCGGGAACAATGATTGTATCAGAACGTGATG
CGAGAGACAATTGTGTTGTTGGAATTGCAAGTGATGTTGGATTTTGCAGTCTTTATGTAAGTAAATTTCTAATGAACAGA
GAAGTTGGATTCGGACGCAAACTTCTCAATATTTTTGAAGATGAAGATATTTCTTTTGAACACGCTCCTTCAGGAATAGA
TGACATGTCCGTTATATTTAGAGATCACAAGTTTACTCCAGAAAAAGAACGTATCGTAATAGACCGTATTAAAAACGAAT
TACATGTGGACACAGTTAGTATTCATCGTGATCTAGCTGTCATTATGGTAGTTGGTGAAGGTCTTGTAAATACAATTGGA
GTTGCTGCCAGAGCTACTTCTTCATTCTCTAAAGCAAGAGTAAATATTGAAATGATTAACCAAGGTTCCTCAGAAGTATC
CATGATGTTTGGTATACACGCAGAAGATATTGATAAAGCCATTCAATCTCTATACCATGAATTTTTTGAAGTGAGCCAAA
AAGTTGAAGAAGCTGAGACAAAAATATTAACGTAA

Upstream 100 bases:

>100_bases
AGACGGATAATTTTCCGTCTTTTTTCAATAAAATTACTTTAATCTTCTATAAAATATGTTATATTAATAAAATAAATTCG
ATAATGGGGGATCATTACAA

Downstream 100 bases:

>100_bases
AAAGACGTCTCTTTATATTTTTGCGTAGTCGGTAATCTTGAAAATTATTTATGTTATTTCCGTACCCTTTAACTAATTCA
TTAGAGAAAATTTATAACAA

Product: aspartate kinase

Products: NA

Alternate protein names: Aspartate kinase [H]

Number of amino acids: Translated: 464; Mature: 464

Protein sequence:

>464_residues
MKVAKFGGSSVANAEQIQKVANIIKADPSRKVVVVSAPGKRFKDDVKITDLLIEFAGSHQRKQFEQSEKTLTIIMDRFKE
IVDNLNLHTSIIDDIKQTIEEDLNKDLSPNQLLDAIKAIGEDSSAKIVSAYLQHIGIDATYMNPKDAGIYVSNEPGHAQL
LPESFPIIKKLRDIDKVVVIPGFFGYAENGELVTFSRGGSDITGSILAAGTKAELYENFTDVDSVYAVNPTIVNNPKEIN
VLTYKEMRELSYAGFSVFHDEALIPAFREQIPVCIKNTNNPDSPGTMIVSERDARDNCVVGIASDVGFCSLYVSKFLMNR
EVGFGRKLLNIFEDEDISFEHAPSGIDDMSVIFRDHKFTPEKERIVIDRIKNELHVDTVSIHRDLAVIMVVGEGLVNTIG
VAARATSSFSKARVNIEMINQGSSEVSMMFGIHAEDIDKAIQSLYHEFFEVSQKVEEAETKILT

Sequences:

>Translated_464_residues
MKVAKFGGSSVANAEQIQKVANIIKADPSRKVVVVSAPGKRFKDDVKITDLLIEFAGSHQRKQFEQSEKTLTIIMDRFKE
IVDNLNLHTSIIDDIKQTIEEDLNKDLSPNQLLDAIKAIGEDSSAKIVSAYLQHIGIDATYMNPKDAGIYVSNEPGHAQL
LPESFPIIKKLRDIDKVVVIPGFFGYAENGELVTFSRGGSDITGSILAAGTKAELYENFTDVDSVYAVNPTIVNNPKEIN
VLTYKEMRELSYAGFSVFHDEALIPAFREQIPVCIKNTNNPDSPGTMIVSERDARDNCVVGIASDVGFCSLYVSKFLMNR
EVGFGRKLLNIFEDEDISFEHAPSGIDDMSVIFRDHKFTPEKERIVIDRIKNELHVDTVSIHRDLAVIMVVGEGLVNTIG
VAARATSSFSKARVNIEMINQGSSEVSMMFGIHAEDIDKAIQSLYHEFFEVSQKVEEAETKILT
>Mature_464_residues
MKVAKFGGSSVANAEQIQKVANIIKADPSRKVVVVSAPGKRFKDDVKITDLLIEFAGSHQRKQFEQSEKTLTIIMDRFKE
IVDNLNLHTSIIDDIKQTIEEDLNKDLSPNQLLDAIKAIGEDSSAKIVSAYLQHIGIDATYMNPKDAGIYVSNEPGHAQL
LPESFPIIKKLRDIDKVVVIPGFFGYAENGELVTFSRGGSDITGSILAAGTKAELYENFTDVDSVYAVNPTIVNNPKEIN
VLTYKEMRELSYAGFSVFHDEALIPAFREQIPVCIKNTNNPDSPGTMIVSERDARDNCVVGIASDVGFCSLYVSKFLMNR
EVGFGRKLLNIFEDEDISFEHAPSGIDDMSVIFRDHKFTPEKERIVIDRIKNELHVDTVSIHRDLAVIMVVGEGLVNTIG
VAARATSSFSKARVNIEMINQGSSEVSMMFGIHAEDIDKAIQSLYHEFFEVSQKVEEAETKILT

Specific function: Catalyzes 2 nonconsecutive reactions in the common biosynthetic pathway leading from Asp to diaminopimelate and Lys, to Met, and to Thr and Ile. [C]

COG id: COG0527

COG function: function code E; Aspartokinases

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 2 ACT domains [H]

Homologues:

Organism=Escherichia coli, GI1786183, Length=479, Percent_Identity=28.6012526096033, Blast_Score=168, Evalue=6e-43,
Organism=Escherichia coli, GI1790455, Length=461, Percent_Identity=24.7288503253796, Blast_Score=109, Evalue=5e-25,
Organism=Escherichia coli, GI1790376, Length=302, Percent_Identity=26.4900662251656, Blast_Score=72, Evalue=5e-14,
Organism=Saccharomyces cerevisiae, GI6320893, Length=501, Percent_Identity=24.3512974051896, Blast_Score=103, Evalue=9e-23,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR002912
- InterPro:   IPR001048
- InterPro:   IPR001341
- InterPro:   IPR018042 [H]

Pfam domain/function: PF00696 AA_kinase; PF01842 ACT [H]

EC number: =2.7.2.4 [H]

Molecular weight: Translated: 51470; Mature: 51470

Theoretical pI: Translated: 4.83; Mature: 4.83

Prosite motif: PS00324 ASPARTOKINASE

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.6 %Cys     (Translated Protein)
2.4 %Met     (Translated Protein)
3.0 %Cys+Met (Translated Protein)
0.6 %Cys     (Mature Protein)
2.4 %Met     (Mature Protein)
3.0 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MKVAKFGGSSVANAEQIQKVANIIKADPSRKVVVVSAPGKRFKDDVKITDLLIEFAGSHQ
CCCCCCCCCCCCCHHHHHHHHHHHHCCCCCEEEEEECCCCCCCCCHHHHHHHHHHHCCHH
RKQFEQSEKTLTIIMDRFKEIVDNLNLHTSIIDDIKQTIEEDLNKDLSPNQLLDAIKAIG
HHHHHHHHHHHHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHCCCCCHHHHHHHHHHHC
EDSSAKIVSAYLQHIGIDATYMNPKDAGIYVSNEPGHAQLLPESFPIIKKLRDIDKVVVI
CCCCHHHHHHHHHHCCCCEEEECCCCCEEEEECCCCCCEECCCCCHHHHHHHCCCEEEEE
PGFFGYAENGELVTFSRGGSDITGSILAAGTKAELYENFTDVDSVYAVNPTIVNNPKEIN
CCCCCCCCCCCEEEEECCCCCCCCHHHCCCCHHHHHHCCCCCCCEEEECCEEECCCCEEE
VLTYKEMRELSYAGFSVFHDEALIPAFREQIPVCIKNTNNPDSPGTMIVSERDARDNCVV
EEEHHHHHHHHHCCHHHHCCCHHHHHHHHCCCEEEECCCCCCCCCEEEEECCCCCCCEEE
GIASDVGFCSLYVSKFLMNREVGFGRKLLNIFEDEDISFEHAPSGIDDMSVIFRDHKFTP
EECCCCHHHHHHHHHHHHHCCCCCCHHHHHHHCCCCCCCCCCCCCCCHHHHHHCCCCCCC
EKERIVIDRIKNELHVDTVSIHRDLAVIMVVGEGLVNTIGVAARATSSFSKARVNIEMIN
HHHHHHHHHHHHHHEEEHEEHHCCEEEEEEECCHHHHHHHHHHHHHCCHHHHEEEEEEEC
QGSSEVSMMFGIHAEDIDKAIQSLYHEFFEVSQKVEEAETKILT
CCCCCEEEEECCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCC
>Mature Secondary Structure
MKVAKFGGSSVANAEQIQKVANIIKADPSRKVVVVSAPGKRFKDDVKITDLLIEFAGSHQ
CCCCCCCCCCCCCHHHHHHHHHHHHCCCCCEEEEEECCCCCCCCCHHHHHHHHHHHCCHH
RKQFEQSEKTLTIIMDRFKEIVDNLNLHTSIIDDIKQTIEEDLNKDLSPNQLLDAIKAIG
HHHHHHHHHHHHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHCCCCCHHHHHHHHHHHC
EDSSAKIVSAYLQHIGIDATYMNPKDAGIYVSNEPGHAQLLPESFPIIKKLRDIDKVVVI
CCCCHHHHHHHHHHCCCCEEEECCCCCEEEEECCCCCCEECCCCCHHHHHHHCCCEEEEE
PGFFGYAENGELVTFSRGGSDITGSILAAGTKAELYENFTDVDSVYAVNPTIVNNPKEIN
CCCCCCCCCCCEEEEECCCCCCCCHHHCCCCHHHHHHCCCCCCCEEEECCEEECCCCEEE
VLTYKEMRELSYAGFSVFHDEALIPAFREQIPVCIKNTNNPDSPGTMIVSERDARDNCVV
EEEHHHHHHHHHCCHHHHCCCHHHHHHHHCCCEEEECCCCCCCCCEEEEECCCCCCCEEE
GIASDVGFCSLYVSKFLMNREVGFGRKLLNIFEDEDISFEHAPSGIDDMSVIFRDHKFTP
EECCCCHHHHHHHHHHHHHCCCCCCHHHHHHHCCCCCCCCCCCCCCCHHHHHHCCCCCCC
EKERIVIDRIKNELHVDTVSIHRDLAVIMVVGEGLVNTIGVAARATSSFSKARVNIEMIN
HHHHHHHHHHHHHHEEEHEEHHCCEEEEEEECCHHHHHHHHHHHHHCCHHHHEEEEEEEC
QGSSEVSMMFGIHAEDIDKAIQSLYHEFFEVSQKVEEAETKILT
CCCCCEEEEECCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 8969502; 9384377 [H]