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The map label for this gene is dinB

Identifier: 23099313

GI number: 23099313

Start: 1888149

End: 1889408

Strand: Reverse

Name: dinB

Synonym: OB1858

Alternate gene names: 23099313

Gene position: 1889408-1888149 (Counterclockwise)

Preceding gene: 23099314

Following gene: 23099309

Centisome position: 52.04

GC content: 35.4

Gene sequence:

>1260_bases
ATGCAACCATCTAAAGTAAACAAACATCGTATTATCTTTCATATTGATATGAATTGTTTTTATGCTTCTGTAGAAATGGC
GCATAATCCATCATTAAAAGGAAAACCACTTGCTATTGCAGGAAACCCTGAAGAAAGAAAGGGAATTATTGTAACGAGTA
GTTATGAAGCGAGAGGAAAAGGTGTAAAAACTACGATGCCGATATGGCAAGCGAAAAAGCTTTGCCCAGATTTGATCCTT
ATGCGTCCAAATTTTGACCGTTATCGAGCAGCATCACGTGAAATTTTTAAAATGCTAGCAGAGATTACGCCATATGTACA
GCCGGTATCCATAGATGAAGGATATATGGATATTACTGATACGATTTATGCGAAAGACCCATTGGTTACAGCGAATCAAT
TACAACAGCGAATTTTGAGCGGACTTGATATTCCATGTAGTATTGGGATAGCACCAAATAAATTTCTTGCTAAAATGGCA
TCGGACATGAAGAAGCCGCTCGGTATAACAGTGCTTCGCAAGAGAGAAGTAGAGAAATTATTATGGCCAATGTCGGTGGA
AGAGATGTACGGAATCGGAGAAAAAACAGCACAGAAGTTAAATAGTATAGAAATTAAAACAATTGGAGATCTCGCTAAAA
AGAATGTATATGAATTAAAACAACTGTTAGGTGTAAACGGAGAACGATTGCAAAACCGTGCCAATGGAATAGATAATCGT
TTAGTAGATCCTGAAGCCGTTCACGACTTTAAAAGCATTGGCAGTTCCCAAACTTTGCCACACGACTCTACAGATGTGAC
AGAATTAATGCAGTTAATCCATGAACTAGTAGACAATGTAGAGCGTCGAGTGAAACGGAAAGAAGCTGCCGGTAAAACGG
TACAAATAACAATTCGATATCATGATAGAAAGACGATTACGAGGAGTAAAAAGTTATATAACTATATTGATAATCACAGA
GAGATATTATTTGTGGCAAAAGAGCTTTTTGAACAACATTGGAATGAAGCGCCTGTCCGTTTATTAGGTGTTTCATTACA
GGATATGGAAACAAAAAGAAATATAGGAGAACAGTTAGATTTATTCACTTACGAAGCAATTGAGAAGAAAGAAAAGCTTA
AAGTAACAGTTGATAAATTAACAAAAAAATATGGTTCGAATATTATTACTTCACAAAAAAATAAAAATGAGAGTCAAGAA
AATCAGCAACCACGTACAAGTTTTCAAAAAGATTTTCTAGATGATTATAAAAAGCCATAA

Upstream 100 bases:

>100_bases
GAAATTGTTAAGGAAGCTTCAAATCAGTAAAATGAAGTGGAGGATTTTGGCACCCTATTCGTAGTGAAAGGGTGCTTTTC
CTCATAAAGGAAGGTTATTT

Downstream 100 bases:

>100_bases
ATCCAATTCTATATGAAGAATGGAAATATGGCTTTTTAGTATGGTAGTAAGTAATGCTTACCTATATGAACGTATTTGAT
TCATGTTATGTTAAAAACTT

Product: DNA polymerase IV

Products: NA

Alternate protein names: Pol IV

Number of amino acids: Translated: 419; Mature: 419

Protein sequence:

>419_residues
MQPSKVNKHRIIFHIDMNCFYASVEMAHNPSLKGKPLAIAGNPEERKGIIVTSSYEARGKGVKTTMPIWQAKKLCPDLIL
MRPNFDRYRAASREIFKMLAEITPYVQPVSIDEGYMDITDTIYAKDPLVTANQLQQRILSGLDIPCSIGIAPNKFLAKMA
SDMKKPLGITVLRKREVEKLLWPMSVEEMYGIGEKTAQKLNSIEIKTIGDLAKKNVYELKQLLGVNGERLQNRANGIDNR
LVDPEAVHDFKSIGSSQTLPHDSTDVTELMQLIHELVDNVERRVKRKEAAGKTVQITIRYHDRKTITRSKKLYNYIDNHR
EILFVAKELFEQHWNEAPVRLLGVSLQDMETKRNIGEQLDLFTYEAIEKKEKLKVTVDKLTKKYGSNIITSQKNKNESQE
NQQPRTSFQKDFLDDYKKP

Sequences:

>Translated_419_residues
MQPSKVNKHRIIFHIDMNCFYASVEMAHNPSLKGKPLAIAGNPEERKGIIVTSSYEARGKGVKTTMPIWQAKKLCPDLIL
MRPNFDRYRAASREIFKMLAEITPYVQPVSIDEGYMDITDTIYAKDPLVTANQLQQRILSGLDIPCSIGIAPNKFLAKMA
SDMKKPLGITVLRKREVEKLLWPMSVEEMYGIGEKTAQKLNSIEIKTIGDLAKKNVYELKQLLGVNGERLQNRANGIDNR
LVDPEAVHDFKSIGSSQTLPHDSTDVTELMQLIHELVDNVERRVKRKEAAGKTVQITIRYHDRKTITRSKKLYNYIDNHR
EILFVAKELFEQHWNEAPVRLLGVSLQDMETKRNIGEQLDLFTYEAIEKKEKLKVTVDKLTKKYGSNIITSQKNKNESQE
NQQPRTSFQKDFLDDYKKP
>Mature_419_residues
MQPSKVNKHRIIFHIDMNCFYASVEMAHNPSLKGKPLAIAGNPEERKGIIVTSSYEARGKGVKTTMPIWQAKKLCPDLIL
MRPNFDRYRAASREIFKMLAEITPYVQPVSIDEGYMDITDTIYAKDPLVTANQLQQRILSGLDIPCSIGIAPNKFLAKMA
SDMKKPLGITVLRKREVEKLLWPMSVEEMYGIGEKTAQKLNSIEIKTIGDLAKKNVYELKQLLGVNGERLQNRANGIDNR
LVDPEAVHDFKSIGSSQTLPHDSTDVTELMQLIHELVDNVERRVKRKEAAGKTVQITIRYHDRKTITRSKKLYNYIDNHR
EILFVAKELFEQHWNEAPVRLLGVSLQDMETKRNIGEQLDLFTYEAIEKKEKLKVTVDKLTKKYGSNIITSQKNKNESQE
NQQPRTSFQKDFLDDYKKP

Specific function: Poorly processive, error-prone DNA polymerase involved in untargeted mutagenesis. Copies undamaged DNA at stalled replication forks, which arise in vivo from mismatched or misaligned primer ends. These misaligned primers can be extended by polIV. Exhibits

COG id: COG0389

COG function: function code L; Nucleotidyltransferase/DNA polymerase involved in DNA repair

Gene ontology:

Cell location: Cytoplasm (Probable)

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 umuC domain

Homologues:

Organism=Homo sapiens, GI154350220, Length=351, Percent_Identity=29.3447293447293, Blast_Score=133, Evalue=4e-31,
Organism=Homo sapiens, GI84043967, Length=345, Percent_Identity=28.695652173913, Blast_Score=122, Evalue=8e-28,
Organism=Homo sapiens, GI7706681, Length=346, Percent_Identity=28.6127167630058, Blast_Score=121, Evalue=1e-27,
Organism=Homo sapiens, GI7705344, Length=113, Percent_Identity=46.9026548672566, Blast_Score=113, Evalue=3e-25,
Organism=Homo sapiens, GI5729982, Length=124, Percent_Identity=36.2903225806452, Blast_Score=90, Evalue=4e-18,
Organism=Escherichia coli, GI1786425, Length=351, Percent_Identity=33.9031339031339, Blast_Score=202, Evalue=3e-53,
Organism=Escherichia coli, GI1787432, Length=395, Percent_Identity=25.3164556962025, Blast_Score=105, Evalue=7e-24,
Organism=Caenorhabditis elegans, GI17537959, Length=311, Percent_Identity=27.6527331189711, Blast_Score=115, Evalue=4e-26,
Organism=Caenorhabditis elegans, GI193205700, Length=403, Percent_Identity=26.0545905707196, Blast_Score=106, Evalue=2e-23,
Organism=Caenorhabditis elegans, GI115534089, Length=124, Percent_Identity=36.2903225806452, Blast_Score=78, Evalue=8e-15,
Organism=Caenorhabditis elegans, GI193205702, Length=215, Percent_Identity=29.7674418604651, Blast_Score=71, Evalue=8e-13,
Organism=Saccharomyces cerevisiae, GI6324921, Length=239, Percent_Identity=27.6150627615063, Blast_Score=84, Evalue=5e-17,
Organism=Drosophila melanogaster, GI19923006, Length=342, Percent_Identity=27.1929824561404, Blast_Score=134, Evalue=1e-31,
Organism=Drosophila melanogaster, GI21355641, Length=314, Percent_Identity=31.2101910828025, Blast_Score=117, Evalue=1e-26,
Organism=Drosophila melanogaster, GI24644984, Length=314, Percent_Identity=31.2101910828025, Blast_Score=117, Evalue=1e-26,
Organism=Drosophila melanogaster, GI24668444, Length=124, Percent_Identity=36.2903225806452, Blast_Score=90, Evalue=3e-18,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): DPO4_OCEIH (Q8EQ56)

Other databases:

- EMBL:   BA000028
- RefSeq:   NP_692779.1
- ProteinModelPortal:   Q8EQ56
- SMR:   Q8EQ56
- GeneID:   1018350
- GenomeReviews:   BA000028_GR
- KEGG:   oih:OB1858
- NMPDR:   fig|221109.1.peg.1857
- HOGENOM:   HBG734504
- OMA:   QAMARQE
- ProtClustDB:   PRK01810
- BioCyc:   OIHE221109:OB1858-MONOMER
- BRENDA:   2.7.7.7
- GO:   GO:0005737
- HAMAP:   MF_01113
- InterPro:   IPR017962
- InterPro:   IPR017961
- InterPro:   IPR001126
- InterPro:   IPR017963
- InterPro:   IPR022880
- Gene3D:   G3DSA:3.30.1490.100
- PANTHER:   PTHR11076

Pfam domain/function: PF00817 IMS; SSF100879 DNA_pol_Y-fam_little_finger

EC number: =2.7.7.7

Molecular weight: Translated: 48075; Mature: 48075

Theoretical pI: Translated: 9.77; Mature: 9.77

Prosite motif: PS50173 UMUC

Important sites: ACT_SITE 113-113

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.7 %Cys     (Translated Protein)
3.1 %Met     (Translated Protein)
3.8 %Cys+Met (Translated Protein)
0.7 %Cys     (Mature Protein)
3.1 %Met     (Mature Protein)
3.8 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MQPSKVNKHRIIFHIDMNCFYASVEMAHNPSLKGKPLAIAGNPEERKGIIVTSSYEARGK
CCCCCCCCCEEEEEEECCEEEEEEECCCCCCCCCCCEEEECCCCCCCCEEEECCCCCCCC
GVKTTMPIWQAKKLCPDLILMRPNFDRYRAASREIFKMLAEITPYVQPVSIDEGYMDITD
CCEECCCHHHHHHHCCHHEEECCCCHHHHHHHHHHHHHHHHHCCCCCEEECCCCCCCHHH
TIYAKDPLVTANQLQQRILSGLDIPCSIGIAPNKFLAKMASDMKKPLGITVLRKREVEKL
HHCCCCCCCCHHHHHHHHHCCCCCCEECCCCCHHHHHHHHHHHCCCCCEEHHHHHHHHHH
LWPMSVEEMYGIGEKTAQKLNSIEIKTIGDLAKKNVYELKQLLGVNGERLQNRANGIDNR
HCCCCHHHHHCCCHHHHHHHCCEEEHHHHHHHHHHHHHHHHHHCCCHHHHHHHHCCCCCC
LVDPEAVHDFKSIGSSQTLPHDSTDVTELMQLIHELVDNVERRVKRKEAAGKTVQITIRY
CCCHHHHHHHHHCCCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHCCCEEEEEEEE
HDRKTITRSKKLYNYIDNHREILFVAKELFEQHWNEAPVRLLGVSLQDMETKRNIGEQLD
CCCHHHHHHHHHHHHHHCCHHHHHHHHHHHHHHCCCCCEEEECCCHHHHHHHHHHHHHHH
LFTYEAIEKKEKLKVTVDKLTKKYGSNIITSQKNKNESQENQQPRTSFQKDFLDDYKKP
HHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCCCCHHCCCHHHHHHHHHHHHCCCC
>Mature Secondary Structure
MQPSKVNKHRIIFHIDMNCFYASVEMAHNPSLKGKPLAIAGNPEERKGIIVTSSYEARGK
CCCCCCCCCEEEEEEECCEEEEEEECCCCCCCCCCCEEEECCCCCCCCEEEECCCCCCCC
GVKTTMPIWQAKKLCPDLILMRPNFDRYRAASREIFKMLAEITPYVQPVSIDEGYMDITD
CCEECCCHHHHHHHCCHHEEECCCCHHHHHHHHHHHHHHHHHCCCCCEEECCCCCCCHHH
TIYAKDPLVTANQLQQRILSGLDIPCSIGIAPNKFLAKMASDMKKPLGITVLRKREVEKL
HHCCCCCCCCHHHHHHHHHCCCCCCEECCCCCHHHHHHHHHHHCCCCCEEHHHHHHHHHH
LWPMSVEEMYGIGEKTAQKLNSIEIKTIGDLAKKNVYELKQLLGVNGERLQNRANGIDNR
HCCCCHHHHHCCCHHHHHHHCCEEEHHHHHHHHHHHHHHHHHHCCCHHHHHHHHCCCCCC
LVDPEAVHDFKSIGSSQTLPHDSTDVTELMQLIHELVDNVERRVKRKEAAGKTVQITIRY
CCCHHHHHHHHHCCCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHCCCEEEEEEEE
HDRKTITRSKKLYNYIDNHREILFVAKELFEQHWNEAPVRLLGVSLQDMETKRNIGEQLD
CCCHHHHHHHHHHHHHHCCHHHHHHHHHHHHHHCCCCCEEEECCCHHHHHHHHHHHHHHH
LFTYEAIEKKEKLKVTVDKLTKKYGSNIITSQKNKNESQENQQPRTSFQKDFLDDYKKP
HHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCCCCHHCCCHHHHHHHHHHHHCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 12235376