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The map label for this gene is rluB [H]

Identifier: 23099278

GI number: 23099278

Start: 1859857

End: 1860591

Strand: Reverse

Name: rluB [H]

Synonym: OB1823

Alternate gene names: 23099278

Gene position: 1860591-1859857 (Counterclockwise)

Preceding gene: 23099279

Following gene: 23099277

Centisome position: 51.25

GC content: 37.96

Gene sequence:

>735_bases
ATGACGAACAATGGTGAAAGACTTCAAAAAGTGATTGCGCAGAGCGGGGTTACATCACGAAGAAAAGCAGAACAAATGAT
TCTTGATGGAAAAGTTAAGGTAAATGGAGAGACCGTTCAGGAGATGGGAGTTAAGGTTACAGCAAACGATGAGATTGTTG
TAGACGGTGTTCCACTTGAAAAGGAAACACCTGTTTATTTTCTTTTATACAAGCCAAGAGGAGTAATTTCCAGTGTGAAA
GATGATAAAGGCAGGAAAGTGGTTACCGATTATTTCGAGGCTGTAGAACAACGAATCTTTCCAATTGGAAGATTGGATTA
TGATACTTCAGGAGTTTTACTATTAACCAACGATGGGGATTTTGCTAACTTACTCATGCACCCTCGTCATGAAATAGACA
AGGTATATATTGCGAAGGTAAGAGGTATACCGAATAAGAAGGAATTAGCTTTACTGCAAAAAGGAATACGTTCAGATAAA
GATGTACTTAAAGCAGTTCAAGTGAAGTTACTATCTACGGACCACCAGAAAAAGACTTCCATTATAGAACTAACCTTACA
TGAAGGAAAGAATCGACAAGTCCGTCGTATGTTAGAAGAATTGGGCTATCCAGTCATGAAATTAAAACGTGAACGCTATG
ACTTTATTGGGTTAGAAGGCTTAAATGCAGGGGAATATCGATCATTAACTCCTAAAGAAGTCAACCAATTACGTGCACAA
GCTCAAAAATTGTAA

Upstream 100 bases:

>100_bases
ATAGTTCTTGTTAAAATAATGTTTGGATAATACACTTGATAGCGTTAATATAATATTAACGCTATTATTATGGTTAAATA
CAAAAAGAATGGTGATTAGA

Downstream 100 bases:

>100_bases
GATAATGGTCAAATTTATAAATAGTCTTAAATGATATAATGACCTGGGAGGGAGTGTTACATTGGATATTCAACAAAATA
AGACAAATAAACAGAAGAAA

Product: ribosomal large subunit pseudouridylate synthase

Products: pseudouridine 5'-phosphate; H2O [C]

Alternate protein names: rRNA pseudouridylate synthase B; rRNA-uridine isomerase B [H]

Number of amino acids: Translated: 244; Mature: 243

Protein sequence:

>244_residues
MTNNGERLQKVIAQSGVTSRRKAEQMILDGKVKVNGETVQEMGVKVTANDEIVVDGVPLEKETPVYFLLYKPRGVISSVK
DDKGRKVVTDYFEAVEQRIFPIGRLDYDTSGVLLLTNDGDFANLLMHPRHEIDKVYIAKVRGIPNKKELALLQKGIRSDK
DVLKAVQVKLLSTDHQKKTSIIELTLHEGKNRQVRRMLEELGYPVMKLKRERYDFIGLEGLNAGEYRSLTPKEVNQLRAQ
AQKL

Sequences:

>Translated_244_residues
MTNNGERLQKVIAQSGVTSRRKAEQMILDGKVKVNGETVQEMGVKVTANDEIVVDGVPLEKETPVYFLLYKPRGVISSVK
DDKGRKVVTDYFEAVEQRIFPIGRLDYDTSGVLLLTNDGDFANLLMHPRHEIDKVYIAKVRGIPNKKELALLQKGIRSDK
DVLKAVQVKLLSTDHQKKTSIIELTLHEGKNRQVRRMLEELGYPVMKLKRERYDFIGLEGLNAGEYRSLTPKEVNQLRAQ
AQKL
>Mature_243_residues
TNNGERLQKVIAQSGVTSRRKAEQMILDGKVKVNGETVQEMGVKVTANDEIVVDGVPLEKETPVYFLLYKPRGVISSVKD
DKGRKVVTDYFEAVEQRIFPIGRLDYDTSGVLLLTNDGDFANLLMHPRHEIDKVYIAKVRGIPNKKELALLQKGIRSDKD
VLKAVQVKLLSTDHQKKTSIIELTLHEGKNRQVRRMLEELGYPVMKLKRERYDFIGLEGLNAGEYRSLTPKEVNQLRAQA
QKL

Specific function: Responsible for synthesis of pseudouridine from uracil- 2633 in 23S ribosomal RNA [H]

COG id: COG1187

COG function: function code J; 16S rRNA uridine-516 pseudouridylate synthase and related pseudouridylate synthases

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 S4 RNA-binding domain [H]

Homologues:

Organism=Escherichia coli, GI1788510, Length=232, Percent_Identity=33.1896551724138, Blast_Score=123, Evalue=1e-29,
Organism=Escherichia coli, GI1790453, Length=234, Percent_Identity=35.042735042735, Blast_Score=118, Evalue=4e-28,
Organism=Escherichia coli, GI1787524, Length=242, Percent_Identity=35.1239669421488, Blast_Score=118, Evalue=4e-28,
Organism=Escherichia coli, GI87081838, Length=176, Percent_Identity=31.8181818181818, Blast_Score=94, Evalue=1e-20,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR020103
- InterPro:   IPR006145
- InterPro:   IPR000748
- InterPro:   IPR018496
- InterPro:   IPR002942 [H]

Pfam domain/function: PF00849 PseudoU_synth_2; PF01479 S4 [H]

EC number: 4.2.1.70 [C]

Molecular weight: Translated: 27701; Mature: 27569

Theoretical pI: Translated: 10.00; Mature: 10.00

Prosite motif: PS50889 S4 ; PS01149 PSI_RSU

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.0 %Cys     (Translated Protein)
2.5 %Met     (Translated Protein)
2.5 %Cys+Met (Translated Protein)
0.0 %Cys     (Mature Protein)
2.1 %Met     (Mature Protein)
2.1 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MTNNGERLQKVIAQSGVTSRRKAEQMILDGKVKVNGETVQEMGVKVTANDEIVVDGVPLE
CCCCHHHHHHHHHHCCCCHHHHHHHHHHCCEEEECCHHHHHCCEEEECCCEEEECCCCCC
KETPVYFLLYKPRGVISSVKDDKGRKVVTDYFEAVEQRIFPIGRLDYDTSGVLLLTNDGD
CCCCEEEEEECCCCHHHHCCCCCCCCHHHHHHHHHHHHCCCCCCCCCCCCCEEEEECCCC
FANLLMHPRHEIDKVYIAKVRGIPNKKELALLQKGIRSDKDVLKAVQVKLLSTDHQKKTS
HHHHHHCCCHHHHHEEEEHHCCCCCHHHHHHHHHHCCCCHHHHHHHHHHHHCCCCCCCCE
IIELTLHEGKNRQVRRMLEELGYPVMKLKRERYDFIGLEGLNAGEYRSLTPKEVNQLRAQ
EEEEEECCCCCHHHHHHHHHHCCHHHHHHHHHCCEECCCCCCCCCCCCCCHHHHHHHHHH
AQKL
HHCC
>Mature Secondary Structure 
TNNGERLQKVIAQSGVTSRRKAEQMILDGKVKVNGETVQEMGVKVTANDEIVVDGVPLE
CCCHHHHHHHHHHCCCCHHHHHHHHHHCCEEEECCHHHHHCCEEEECCCEEEECCCCCC
KETPVYFLLYKPRGVISSVKDDKGRKVVTDYFEAVEQRIFPIGRLDYDTSGVLLLTNDGD
CCCCEEEEEECCCCHHHHCCCCCCCCHHHHHHHHHHHHCCCCCCCCCCCCCEEEEECCCC
FANLLMHPRHEIDKVYIAKVRGIPNKKELALLQKGIRSDKDVLKAVQVKLLSTDHQKKTS
HHHHHHCCCHHHHHEEEEHHCCCCCHHHHHHHHHHCCCCHHHHHHHHHHHHCCCCCCCCE
IIELTLHEGKNRQVRRMLEELGYPVMKLKRERYDFIGLEGLNAGEYRSLTPKEVNQLRAQ
EEEEEECCCCCHHHHHHHHHHCCHHHHHHHHHCCEECCCCCCCCCCCCCCHHHHHHHHHH
AQKL
HHCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: uracil; D-ribose phosphate [C]

Specific reaction: uracil + D-ribose phosphate = pseudouridine 5'-phosphate + H2O [C]

General reaction: addition of H2O; elimination of H2O; C-O bond cleavage [C]

Inhibitor: 1-(Tetrahydro-2-furanyl)-5-fluorouracil; 5-fluorouracil [C]

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 7934829; 9384377; 9888802 [H]