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The map label for this gene is aroE

Identifier: 23099235

GI number: 23099235

Start: 1817361

End: 1818650

Strand: Reverse

Name: aroE

Synonym: OB1780

Alternate gene names: 23099235

Gene position: 1818650-1817361 (Counterclockwise)

Preceding gene: 23099236

Following gene: 23099234

Centisome position: 50.09

GC content: 37.44

Gene sequence:

>1290_bases
ATGAACCCACTAGCATTGCAACCTATTTCCGGATCTCTTCATGGAACAATAAAAGTACCTGGAGATAAATCAATATCTCA
TCGTTCGATCATTTTTGGTTCTATTGCAAAAGGAACTACAACTGTTACTAATTTTCTGGATGGCGAAGATTGTATGCGGA
CAATCGATGCATTCAAAGAAATGGGAGTACCAATTCAAAAAAACGGTTCAAATGTTACGATCGAAGGTACAGGTTTAAGT
GGATTAATGGAGCCTAACAAGGAATTAGACTTTGGTAATTCAGGAACAACGACTCGATTAATGTTGGGTTTACTTGCTGG
ACTCCCATTTAATACTACAGTATATGGTGATGCATCGCTTTCTAAAAGGCCAATGAACCGGGTTGTCGATCCCTTGCGTT
TAATGGGAGCAACTATCGATGGTAAAGAGCAAGGCAATTATTTACCTTTAACGATAACAGGCACATCATTAAATTCCATT
GATTATGATTTACCGGTAAAAAGTGCTCAAGTAAAGTCTGCCTTGCTTTTAGCGGGATTACATTCAGAAAATACTACTAT
AGTTCGAGAAAAATCACCAACTCGAAATCATACGGAAACAATGCTACAAGCATTCGGAGCAAATATAAAGTCGGATGGTC
ATACAACATCAATTTCTAAAACTGATGATCTCCAAGGTTGTCATGTAGAAGTGCCTGGTGATATTTCTTCAGCTGCATTT
TTCGTTGTGGCAGCATGTATGGTACCAGGAAGTGAAGTAATATTAAAAGATGTTGGACTCAATGAAACGAGAACCGGGAT
TATCGACATCGTACTTCAAATGGGCGCGAAACTGACAATTCAAAATGAGCGTATTGTAGGTGGAGAAAAAATAGGTGATA
TTCTTATCATCAGTTCACCATTAACTGGAGTTACTATTGAAGGAGACGCGATCCCTCGTCTGATTGATGAAATACCAATT
CTAGCGTTATTAGCAACCCAAGCTGATGGGACAACAATTATTAAAGATGCAGAAGAGTTAAAAGTCAAAGAAACAGATCG
TTTGCTTGCAGTTTCTGAAAATCTCACTCAACTCGGAGCAGATGTAACTCCTACAGAAGATGGGATGATCATTAGGGGAA
ATACAAAACTAAAAGGTGGAAACTTCAAATCCTTTGACGATCATCGAATTGCAATGATGGGGATTATTGCATCATTGGTA
ACAGAAGACACTGTAGTTGTAGACAATATTGATTGTATAAATATTTCTTACCCTAATTTTGTTAATGATCTACAGTCTAT
ATTAAAGTAA

Upstream 100 bases:

>100_bases
AGCTTCCATACCCAAAAAGAACAAATAGGAAGCTCGAAAAAACTAACTTCATTAGGATATGAGACAATGATACAAAATTA
AGAAAAAGGGAGTTATAGAG

Downstream 100 bases:

>100_bases
ACTTCAATACTATAAGATTATGCTATAAAGTCTGTTCAAGGGCATTTAGGTCATATCTTTTGGTAGTAATGTCATAGGAA
ATGAGGAAGAACCCCTTTCT

Product: 3-phosphoshikimate 1-carboxyvinyltransferase

Products: NA

Alternate protein names: 5-enolpyruvylshikimate-3-phosphate synthase; EPSP synthase; EPSPS

Number of amino acids: Translated: 429; Mature: 429

Protein sequence:

>429_residues
MNPLALQPISGSLHGTIKVPGDKSISHRSIIFGSIAKGTTTVTNFLDGEDCMRTIDAFKEMGVPIQKNGSNVTIEGTGLS
GLMEPNKELDFGNSGTTTRLMLGLLAGLPFNTTVYGDASLSKRPMNRVVDPLRLMGATIDGKEQGNYLPLTITGTSLNSI
DYDLPVKSAQVKSALLLAGLHSENTTIVREKSPTRNHTETMLQAFGANIKSDGHTTSISKTDDLQGCHVEVPGDISSAAF
FVVAACMVPGSEVILKDVGLNETRTGIIDIVLQMGAKLTIQNERIVGGEKIGDILIISSPLTGVTIEGDAIPRLIDEIPI
LALLATQADGTTIIKDAEELKVKETDRLLAVSENLTQLGADVTPTEDGMIIRGNTKLKGGNFKSFDDHRIAMMGIIASLV
TEDTVVVDNIDCINISYPNFVNDLQSILK

Sequences:

>Translated_429_residues
MNPLALQPISGSLHGTIKVPGDKSISHRSIIFGSIAKGTTTVTNFLDGEDCMRTIDAFKEMGVPIQKNGSNVTIEGTGLS
GLMEPNKELDFGNSGTTTRLMLGLLAGLPFNTTVYGDASLSKRPMNRVVDPLRLMGATIDGKEQGNYLPLTITGTSLNSI
DYDLPVKSAQVKSALLLAGLHSENTTIVREKSPTRNHTETMLQAFGANIKSDGHTTSISKTDDLQGCHVEVPGDISSAAF
FVVAACMVPGSEVILKDVGLNETRTGIIDIVLQMGAKLTIQNERIVGGEKIGDILIISSPLTGVTIEGDAIPRLIDEIPI
LALLATQADGTTIIKDAEELKVKETDRLLAVSENLTQLGADVTPTEDGMIIRGNTKLKGGNFKSFDDHRIAMMGIIASLV
TEDTVVVDNIDCINISYPNFVNDLQSILK
>Mature_429_residues
MNPLALQPISGSLHGTIKVPGDKSISHRSIIFGSIAKGTTTVTNFLDGEDCMRTIDAFKEMGVPIQKNGSNVTIEGTGLS
GLMEPNKELDFGNSGTTTRLMLGLLAGLPFNTTVYGDASLSKRPMNRVVDPLRLMGATIDGKEQGNYLPLTITGTSLNSI
DYDLPVKSAQVKSALLLAGLHSENTTIVREKSPTRNHTETMLQAFGANIKSDGHTTSISKTDDLQGCHVEVPGDISSAAF
FVVAACMVPGSEVILKDVGLNETRTGIIDIVLQMGAKLTIQNERIVGGEKIGDILIISSPLTGVTIEGDAIPRLIDEIPI
LALLATQADGTTIIKDAEELKVKETDRLLAVSENLTQLGADVTPTEDGMIIRGNTKLKGGNFKSFDDHRIAMMGIIASLV
TEDTVVVDNIDCINISYPNFVNDLQSILK

Specific function: Aromatic amino acids biosynthesis; shikimate pathway; sixth step. [C]

COG id: COG0128

COG function: function code E; 5-enolpyruvylshikimate-3-phosphate synthase

Gene ontology:

Cell location: Cytoplasm

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the EPSP synthase family

Homologues:

Organism=Escherichia coli, GI1787137, Length=440, Percent_Identity=29.5454545454545, Blast_Score=154, Evalue=1e-38,
Organism=Saccharomyces cerevisiae, GI6320332, Length=460, Percent_Identity=26.304347826087, Blast_Score=110, Evalue=4e-25,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): AROA_OCEIH (Q8EQC1)

Other databases:

- EMBL:   BA000028
- RefSeq:   NP_692701.1
- ProteinModelPortal:   Q8EQC1
- SMR:   Q8EQC1
- GeneID:   1018274
- GenomeReviews:   BA000028_GR
- KEGG:   oih:OB1780
- NMPDR:   fig|221109.1.peg.1781
- HOGENOM:   HBG646626
- OMA:   LSRNHTE
- ProtClustDB:   PRK02427
- BioCyc:   OIHE221109:OB1780-MONOMER
- BRENDA:   2.5.1.19
- GO:   GO:0005737
- HAMAP:   MF_00210
- InterPro:   IPR001986
- InterPro:   IPR006264
- InterPro:   IPR023193
- InterPro:   IPR013792
- Gene3D:   G3DSA:3.65.10.10
- PIRSF:   PIRSF000505
- TIGRFAMs:   TIGR01356

Pfam domain/function: PF00275 EPSP_synthase; SSF55205 RNA3'_cycl/enolpyr_transf_A/B

EC number: =2.5.1.19

Molecular weight: Translated: 45744; Mature: 45744

Theoretical pI: Translated: 4.73; Mature: 4.73

Prosite motif: PS00104 EPSP_SYNTHASE_1; PS00885 EPSP_SYNTHASE_2

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.9 %Cys     (Translated Protein)
3.0 %Met     (Translated Protein)
4.0 %Cys+Met (Translated Protein)
0.9 %Cys     (Mature Protein)
3.0 %Met     (Mature Protein)
4.0 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MNPLALQPISGSLHGTIKVPGDKSISHRSIIFGSIAKGTTTVTNFLDGEDCMRTIDAFKE
CCCCEECCCCCCCEEEEECCCCCCCCCCEEEEECCCCCCCHHHHCCCCHHHHHHHHHHHH
MGVPIQKNGSNVTIEGTGLSGLMEPNKELDFGNSGTTTRLMLGLLAGLPFNTTVYGDASL
CCCCEEECCCEEEEEECCCCCCCCCCCCCCCCCCCCHHHHHHHHHHCCCCCEEEECCCCC
SKRPMNRVVDPLRLMGATIDGKEQGNYLPLTITGTSLNSIDYDLPVKSAQVKSALLLAGL
CCCCHHHHHHHHHHHCCCCCCCCCCCEEEEEEECCCCCCCCCCCCCCHHHHHHHHEEEEC
HSENTTIVREKSPTRNHTETMLQAFGANIKSDGHTTSISKTDDLQGCHVEVPGDISSAAF
CCCCCEEEECCCCCCHHHHHHHHHHCCCCCCCCCEEECCCCCCCCCCEEECCCCCHHHHH
FVVAACMVPGSEVILKDVGLNETRTGIIDIVLQMGAKLTIQNERIVGGEKIGDILIISSP
HHHHHHHCCCCCEEEEECCCCCHHHHHHHHHHHCCCEEEEECCEEECCCCCCCEEEEECC
LTGVTIEGDAIPRLIDEIPILALLATQADGTTIIKDAEELKVKETDRLLAVSENLTQLGA
CCCEEECCCHHHHHHHHCCEEEEEEECCCCCEEECCHHHCEEHHCCCEEEEHHHHHHHCC
DVTPTEDGMIIRGNTKLKGGNFKSFDDHRIAMMGIIASLVTEDTVVVDNIDCINISYPNF
CCCCCCCCEEEECCCEECCCCCCCCCCCHHHHHHHHHHHHCCCEEEEECCEEEEECCCHH
VNDLQSILK
HHHHHHHHC
>Mature Secondary Structure
MNPLALQPISGSLHGTIKVPGDKSISHRSIIFGSIAKGTTTVTNFLDGEDCMRTIDAFKE
CCCCEECCCCCCCEEEEECCCCCCCCCCEEEEECCCCCCCHHHHCCCCHHHHHHHHHHHH
MGVPIQKNGSNVTIEGTGLSGLMEPNKELDFGNSGTTTRLMLGLLAGLPFNTTVYGDASL
CCCCEEECCCEEEEEECCCCCCCCCCCCCCCCCCCCHHHHHHHHHHCCCCCEEEECCCCC
SKRPMNRVVDPLRLMGATIDGKEQGNYLPLTITGTSLNSIDYDLPVKSAQVKSALLLAGL
CCCCHHHHHHHHHHHCCCCCCCCCCCEEEEEEECCCCCCCCCCCCCCHHHHHHHHEEEEC
HSENTTIVREKSPTRNHTETMLQAFGANIKSDGHTTSISKTDDLQGCHVEVPGDISSAAF
CCCCCEEEECCCCCCHHHHHHHHHHCCCCCCCCCEEECCCCCCCCCCEEECCCCCHHHHH
FVVAACMVPGSEVILKDVGLNETRTGIIDIVLQMGAKLTIQNERIVGGEKIGDILIISSP
HHHHHHHCCCCCEEEEECCCCCHHHHHHHHHHHCCCEEEEECCEEECCCCCCCEEEEECC
LTGVTIEGDAIPRLIDEIPILALLATQADGTTIIKDAEELKVKETDRLLAVSENLTQLGA
CCCEEECCCHHHHHHHHCCEEEEEEECCCCCEEECCHHHCEEHHCCCEEEEHHHHHHHCC
DVTPTEDGMIIRGNTKLKGGNFKSFDDHRIAMMGIIASLVTEDTVVVDNIDCINISYPNF
CCCCCCCCEEEECCCEECCCCCCCCCCCHHHHHHHHHHHHCCCEEEEECCEEEEECCCHH
VNDLQSILK
HHHHHHHHC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 12235376