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The map label for this gene is lytF [H]

Identifier: 23099196

GI number: 23099196

Start: 1781331

End: 1782350

Strand: Direct

Name: lytF [H]

Synonym: OB1741

Alternate gene names: 23099196

Gene position: 1781331-1782350 (Clockwise)

Preceding gene: 23099186

Following gene: 23099198

Centisome position: 49.07

GC content: 34.61

Gene sequence:

>1020_bases
TTGAATACCACGATAGAACAAATAATTAAAAATTCTATTGTATATACATATGTTGCTAGTCAACCATTTGTTGCAATGAC
TGGATTACCAGTTGATATAGAAGCATCTGAACAAGTAGTACAAAGCGATAATCATATCGAATCTTCAAATGCATTAATAA
AAAAGGAAGATGTTTGGTTTGGCGAAAATTGGAATACGGAACAATATAGCATAAGTGAAGAAGAATCAACATTTCAAATA
GATGATGAGCAATTTAATGATGCACTTAGTATTTCTCAATTAGAAGAAATAGAAAAAGCCAACTCGATTTCTATAATTGA
AGATAAGATCAACTCCATTCATCCAGGAATGAAATCGGATGAAATAAAAGAAGTCCAAAAGGCATTAGTTACTCTGGATT
ATTATTCAGATGAAATAGATGGTATTTATGGTCCTTTAACCCATGATGCTTTAGTTAGTATAGAGAAAGATTATGACTTA
TCATTGATAAGTGAGCAACAAGACGAAAGAATTCAAGAGATATATTATGCAGTGGAAGAAGAACCCCAGAATTCAAGTGT
AGACACAGTGGAAGGTAATGCATCTTCTACTTCTGAAAATAATGATGATATGAGCGAAGAGCAAAGTAAGGATCCCTACT
ATTCAAAACAACTCGATGTAAGCGGAAATAAACAGCCTATTATAAAAGCTGCTCGATCATTAGTCGGGACCCCTTATGTG
TATGGCGGTGATGGACCAGGAGGTTTTGATTGTAGTGGTTTCATTCAATATGTCTATGAAAGCCATGATATCGTTATCCC
TAGAACAGTTAATGAAATATGGAATTTTAGTTCTCCTGTTGATAGTCCTTCGATTGGTGATATCGTATTTTTTTCAACCA
CACACTCAGGGCCATCCCATGCAGGTATTTATATTGGTAATGGGCAATTTATTCATGCTGGTTCATCTAGTGGTGTCGTT
ATTACCGAGTTATCCAATCCTTATTGGGAAGAGAGATATTTAGGTGCAAAGCGTATCTAA

Upstream 100 bases:

>100_bases
AGATACTAGTCCTCTTTTGTTACATAATCGTAAAACATAAGCTCACTATTCGAACATAAAACTAGAGTAAAGCTCGGTTT
TTCAGAGGAGGTTCACAAAT

Downstream 100 bases:

>100_bases
TAGTATAGAGTTATTCCCCTTCCCATTCTTTGTAGAATTGATGTAGAAAGTTTTCGATGTACTGATGACGTTCTGCTGCT
ATCATTCTACCTGTATTTGT

Product: cell wall lytic activity

Products: NA

Alternate protein names: Cell wall-associated polypeptide CWBP49; Gamma-D-glutamate-meso-diaminopimelate muropeptidase lytF [H]

Number of amino acids: Translated: 339; Mature: 339

Protein sequence:

>339_residues
MNTTIEQIIKNSIVYTYVASQPFVAMTGLPVDIEASEQVVQSDNHIESSNALIKKEDVWFGENWNTEQYSISEEESTFQI
DDEQFNDALSISQLEEIEKANSISIIEDKINSIHPGMKSDEIKEVQKALVTLDYYSDEIDGIYGPLTHDALVSIEKDYDL
SLISEQQDERIQEIYYAVEEEPQNSSVDTVEGNASSTSENNDDMSEEQSKDPYYSKQLDVSGNKQPIIKAARSLVGTPYV
YGGDGPGGFDCSGFIQYVYESHDIVIPRTVNEIWNFSSPVDSPSIGDIVFFSTTHSGPSHAGIYIGNGQFIHAGSSSGVV
ITELSNPYWEERYLGAKRI

Sequences:

>Translated_339_residues
MNTTIEQIIKNSIVYTYVASQPFVAMTGLPVDIEASEQVVQSDNHIESSNALIKKEDVWFGENWNTEQYSISEEESTFQI
DDEQFNDALSISQLEEIEKANSISIIEDKINSIHPGMKSDEIKEVQKALVTLDYYSDEIDGIYGPLTHDALVSIEKDYDL
SLISEQQDERIQEIYYAVEEEPQNSSVDTVEGNASSTSENNDDMSEEQSKDPYYSKQLDVSGNKQPIIKAARSLVGTPYV
YGGDGPGGFDCSGFIQYVYESHDIVIPRTVNEIWNFSSPVDSPSIGDIVFFSTTHSGPSHAGIYIGNGQFIHAGSSSGVV
ITELSNPYWEERYLGAKRI
>Mature_339_residues
MNTTIEQIIKNSIVYTYVASQPFVAMTGLPVDIEASEQVVQSDNHIESSNALIKKEDVWFGENWNTEQYSISEEESTFQI
DDEQFNDALSISQLEEIEKANSISIIEDKINSIHPGMKSDEIKEVQKALVTLDYYSDEIDGIYGPLTHDALVSIEKDYDL
SLISEQQDERIQEIYYAVEEEPQNSSVDTVEGNASSTSENNDDMSEEQSKDPYYSKQLDVSGNKQPIIKAARSLVGTPYV
YGGDGPGGFDCSGFIQYVYESHDIVIPRTVNEIWNFSSPVDSPSIGDIVFFSTTHSGPSHAGIYIGNGQFIHAGSSSGVV
ITELSNPYWEERYLGAKRI

Specific function: Cleaves gamma-D-glutamate-meso-diaminopimelate bonds. Cell wall hydrolase involved in cell autolysis [H]

COG id: COG0791

COG function: function code M; Cell wall-associated hydrolases (invasion-associated proteins)

Gene ontology:

Cell location: Secreted, cell wall. Note=LysM repeats are thought to be involved in peptidoglycan binding [H]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 5 LysM repeats [H]

Homologues:

Organism=Escherichia coli, GI1787944, Length=115, Percent_Identity=41.7391304347826, Blast_Score=97, Evalue=1e-21,
Organism=Escherichia coli, GI1788501, Length=107, Percent_Identity=41.1214953271028, Blast_Score=92, Evalue=5e-20,
Organism=Escherichia coli, GI1786421, Length=114, Percent_Identity=37.719298245614, Blast_Score=86, Evalue=5e-18,
Organism=Escherichia coli, GI1788001, Length=109, Percent_Identity=41.2844036697248, Blast_Score=79, Evalue=4e-16,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR000064
- InterPro:   IPR018392
- InterPro:   IPR002482 [H]

Pfam domain/function: PF01476 LysM; PF00877 NLPC_P60 [H]

EC number: NA

Molecular weight: Translated: 37756; Mature: 37756

Theoretical pI: Translated: 3.92; Mature: 3.92

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.3 %Cys     (Translated Protein)
1.2 %Met     (Translated Protein)
1.5 %Cys+Met (Translated Protein)
0.3 %Cys     (Mature Protein)
1.2 %Met     (Mature Protein)
1.5 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MNTTIEQIIKNSIVYTYVASQPFVAMTGLPVDIEASEQVVQSDNHIESSNALIKKEDVWF
CCCHHHHHHHCCEEEEEECCCCEEEECCCCCCCCCHHHHHHCCCCCCCCCCEEEECCEEE
GENWNTEQYSISEEESTFQIDDEQFNDALSISQLEEIEKANSISIIEDKINSIHPGMKSD
CCCCCCCCEECCCCCCEEEECHHHHCCHHHHHHHHHHHHCCCEEHHHHHHHHHCCCCCCH
EIKEVQKALVTLDYYSDEIDGIYGPLTHDALVSIEKDYDLSLISEQQDERIQEIYYAVEE
HHHHHHHHHHHHHHCCCCCCCCCCCCCCCCEEEECCCCCCEECCCHHHHHHHHHHHHHHC
EPQNSSVDTVEGNASSTSENNDDMSEEQSKDPYYSKQLDVSGNKQPIIKAARSLVGTPYV
CCCCCCCCEECCCCCCCCCCCCCCCHHHCCCCCCCEEECCCCCCCHHHHHHHHHHCCCEE
YGGDGPGGFDCSGFIQYVYESHDIVIPRTVNEIWNFSSPVDSPSIGDIVFFSTTHSGPSH
ECCCCCCCCCHHHHHHHHHCCCCEEECCCHHHHHCCCCCCCCCCCCCEEEEEECCCCCCC
AGIYIGNGQFIHAGSSSGVVITELSNPYWEERYLGAKRI
CEEEECCCEEEEECCCCCEEEEECCCCCHHHHHCCCCCC
>Mature Secondary Structure
MNTTIEQIIKNSIVYTYVASQPFVAMTGLPVDIEASEQVVQSDNHIESSNALIKKEDVWF
CCCHHHHHHHCCEEEEEECCCCEEEECCCCCCCCCHHHHHHCCCCCCCCCCEEEECCEEE
GENWNTEQYSISEEESTFQIDDEQFNDALSISQLEEIEKANSISIIEDKINSIHPGMKSD
CCCCCCCCEECCCCCCEEEECHHHHCCHHHHHHHHHHHHCCCEEHHHHHHHHHCCCCCCH
EIKEVQKALVTLDYYSDEIDGIYGPLTHDALVSIEKDYDLSLISEQQDERIQEIYYAVEE
HHHHHHHHHHHHHHCCCCCCCCCCCCCCCCEEEECCCCCCEECCCHHHHHHHHHHHHHHC
EPQNSSVDTVEGNASSTSENNDDMSEEQSKDPYYSKQLDVSGNKQPIIKAARSLVGTPYV
CCCCCCCCEECCCCCCCCCCCCCCCHHHCCCCCCCEEECCCCCCCHHHHHHHHHHCCCEE
YGGDGPGGFDCSGFIQYVYESHDIVIPRTVNEIWNFSSPVDSPSIGDIVFFSTTHSGPSH
ECCCCCCCCCHHHHHHHHHCCCCEEECCCHHHHHCCCCCCCCCCCCCEEEEEECCCCCCC
AGIYIGNGQFIHAGSSSGVVITELSNPYWEERYLGAKRI
CEEEECCCEEEEECCCCCEEEEECCCCCHHHHHCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 9579061; 9384377; 10206711 [H]