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The map label for this gene is lexA

Identifier: 23099124

GI number: 23099124

Start: 1710717

End: 1711340

Strand: Reverse

Name: lexA

Synonym: OB1669

Alternate gene names: 23099124

Gene position: 1711340-1710717 (Counterclockwise)

Preceding gene: 23099134

Following gene: 23099123

Centisome position: 47.14

GC content: 36.22

Gene sequence:

>624_bases
ATGACAAAACTATCAAAAAGACAACAAATGATCTTTGACTTCATTAAAAGCGAAGTTAAGTTAAAAGGATATCCACCATC
AGTTCGAGAAATTGCCGTAGCAGTCGGACTCGCTTCCAGTTCAACTGTCCACGGACATTTAGAGAGATTGGAAAATAAAG
GATATATAAGAAGAGATCCTACTAAACCAAGAGCAATTGAAATTATTGATTTAGAAATGGAGCAACAACTACCAAAAGAT
GAGGCACGGTATGCACCTGTAATTGGTAAAGTAACGGCAGGGATACCAATTACAGCTGTTGAAAATATTGAAGAGTTCGT
CCCAATTCCAAGTTCAAGTGCCGGTCCTGACGATAATGTATTTGTACTTGTAATTGATGGTGAAAGTATGATTGAGGCAG
GCATATTAGATGGAGACATGGTAATTGTTAAACAACAGAACACAGCAGTGAATGGAGAAATAGTTGTAGCGATGACCGAG
GAGAATGAAGCAACAGTAAAACGATTCTTTAAAGAAGAAAATCGTATTCGACTTCAACCAGAAAATGCCACAATGGAACC
ATTATTTTATGATAATGTAACAATCCTTGGAAAAGTGATTGGATTATATCGAAATATACATTAA

Upstream 100 bases:

>100_bases
TATGATCTTGTCAACAAAAAATTAGAACGAGTGTTTGCTTATTTTATAGTAAAATGATAAACTATCATTAATAAAAACCT
ATGCTAGAGGTGAACGTTTG

Downstream 100 bases:

>100_bases
AAAAAAGAGTTTAGCTAAAATTCTATAGCTAAACTCTTTTTTTATATGCAAAGAATTATTCATCTACATAGTTTGTACAT
AAATTTGCAATCAAACTAAT

Product: LexA repressor

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 207; Mature: 206

Protein sequence:

>207_residues
MTKLSKRQQMIFDFIKSEVKLKGYPPSVREIAVAVGLASSSTVHGHLERLENKGYIRRDPTKPRAIEIIDLEMEQQLPKD
EARYAPVIGKVTAGIPITAVENIEEFVPIPSSSAGPDDNVFVLVIDGESMIEAGILDGDMVIVKQQNTAVNGEIVVAMTE
ENEATVKRFFKEENRIRLQPENATMEPLFYDNVTILGKVIGLYRNIH

Sequences:

>Translated_207_residues
MTKLSKRQQMIFDFIKSEVKLKGYPPSVREIAVAVGLASSSTVHGHLERLENKGYIRRDPTKPRAIEIIDLEMEQQLPKD
EARYAPVIGKVTAGIPITAVENIEEFVPIPSSSAGPDDNVFVLVIDGESMIEAGILDGDMVIVKQQNTAVNGEIVVAMTE
ENEATVKRFFKEENRIRLQPENATMEPLFYDNVTILGKVIGLYRNIH
>Mature_206_residues
TKLSKRQQMIFDFIKSEVKLKGYPPSVREIAVAVGLASSSTVHGHLERLENKGYIRRDPTKPRAIEIIDLEMEQQLPKDE
ARYAPVIGKVTAGIPITAVENIEEFVPIPSSSAGPDDNVFVLVIDGESMIEAGILDGDMVIVKQQNTAVNGEIVVAMTEE
NEATVKRFFKEENRIRLQPENATMEPLFYDNVTILGKVIGLYRNIH

Specific function: Represses a number of genes involved in the response to DNA damage (SOS response), including recA and lexA. In the presence of single-stranded DNA, recA interacts with lexA causing an autocatalytic cleavage which disrupts the DNA-binding part of lexA, lea

COG id: COG1974

COG function: function code KT; SOS-response transcriptional repressors (RecA-mediated autopeptidases)

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the peptidase S24 family

Homologues:

Organism=Escherichia coli, GI1790476, Length=209, Percent_Identity=33.9712918660287, Blast_Score=114, Evalue=3e-27,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): LEXA_OCEIH (Q8EQM5)

Other databases:

- EMBL:   BA000028
- RefSeq:   NP_692590.1
- ProteinModelPortal:   Q8EQM5
- SMR:   Q8EQM5
- MEROPS:   S24.001
- GeneID:   1018165
- GenomeReviews:   BA000028_GR
- KEGG:   oih:OB1669
- NMPDR:   fig|221109.1.peg.1672
- HOGENOM:   HBG679610
- OMA:   IAENPDF
- ProtClustDB:   PRK00215
- BioCyc:   OIHE221109:OB1669-MONOMER
- BRENDA:   3.4.21.88
- GO:   GO:0006508
- GO:   GO:0006350
- HAMAP:   MF_00015
- InterPro:   IPR006199
- InterPro:   IPR006200
- InterPro:   IPR006197
- InterPro:   IPR019759
- InterPro:   IPR015927
- InterPro:   IPR011056
- InterPro:   IPR011991
- Gene3D:   G3DSA:2.10.109.10
- Gene3D:   G3DSA:1.10.10.10
- PRINTS:   PR00726
- TIGRFAMs:   TIGR00498

Pfam domain/function: PF01726 LexA_DNA_bind; PF00717 Peptidase_S24; SSF51306 Pept_S24_S26_C

EC number: =3.4.21.88

Molecular weight: Translated: 23093; Mature: 22962

Theoretical pI: Translated: 4.87; Mature: 4.87

Prosite motif: NA

Important sites: ACT_SITE 129-129 ACT_SITE 167-167

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.0 %Cys     (Translated Protein)
3.4 %Met     (Translated Protein)
3.4 %Cys+Met (Translated Protein)
0.0 %Cys     (Mature Protein)
2.9 %Met     (Mature Protein)
2.9 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MTKLSKRQQMIFDFIKSEVKLKGYPPSVREIAVAVGLASSSTVHGHLERLENKGYIRRDP
CCCCHHHHHHHHHHHHHCEEECCCCCHHHHHEEHEECCCCCCHHHHHHHHHCCCCEEECC
TKPRAIEIIDLEMEQQLPKDEARYAPVIGKVTAGIPITAVENIEEFVPIPSSSAGPDDNV
CCCCEEEEEEECHHHHCCCCHHCCCCEEEEHCCCCCCHHHHHHHHHCCCCCCCCCCCCCE
FVLVIDGESMIEAGILDGDMVIVKQQNTAVNGEIVVAMTEENEATVKRFFKEENRIRLQP
EEEEECCCHHHHHCCCCCCEEEEEECCCCCCCEEEEEEECCCHHHHHHHHHCCCCEEECC
ENATMEPLFYDNVTILGKVIGLYRNIH
CCCCCCEEEECCHHHHHHHHHHHHCCC
>Mature Secondary Structure 
TKLSKRQQMIFDFIKSEVKLKGYPPSVREIAVAVGLASSSTVHGHLERLENKGYIRRDP
CCCHHHHHHHHHHHHHCEEECCCCCHHHHHEEHEECCCCCCHHHHHHHHHCCCCEEECC
TKPRAIEIIDLEMEQQLPKDEARYAPVIGKVTAGIPITAVENIEEFVPIPSSSAGPDDNV
CCCCEEEEEEECHHHHCCCCHHCCCCEEEEHCCCCCCHHHHHHHHHCCCCCCCCCCCCCE
FVLVIDGESMIEAGILDGDMVIVKQQNTAVNGEIVVAMTEENEATVKRFFKEENRIRLQP
EEEEECCCHHHHHCCCCCCEEEEEECCCCCCCEEEEEEECCCHHHHHHHHHCCCCEEECC
ENATMEPLFYDNVTILGKVIGLYRNIH
CCCCCCEEEECCHHHHHHHHHHHHCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 12235376