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The map label for this gene is sigD [H]

Identifier: 23099037

GI number: 23099037

Start: 1619437

End: 1620213

Strand: Direct

Name: sigD [H]

Synonym: OB1582

Alternate gene names: 23099037

Gene position: 1619437-1620213 (Clockwise)

Preceding gene: 23099036

Following gene: 23099038

Centisome position: 44.61

GC content: 32.82

Gene sequence:

>777_bases
GTGAATATGACAAATTCTCCTCTGGAACAAAAGTTATGGGACAAATGGAAAAATCATCAAGATGGGGAAGCGGCCAATCA
ATTGATTAATAATTATATGTATCTTGTTAACTATCATACTGAGAGAATAGCAAGTCACCTTCCGAATAATGTAGTGAAAG
ATGATATTAGAAGTTTTGGAATTCTCGGTCTATATGATGCTCTTAAGAAATTTGATCCTTCCAGAGATTTGAAATTTGAT
ACGTATGCTTCTTTTCGAATAAAAGGTTCTATTATGGATGGTTTAAGAAAAGAAGATTGGTTACCTCGATCTATACGAGA
AAAAACAAAAAAAGTTGAACAAGCAATGGAAGAATATGAACAAAGCTATCAAAGGGAAGCAAATTCATCAGAGATTGCTA
ATCTAACAGGAATGACAACTGAAGAAGTAGAAACTACGATGAAAGATGCGTTATTTAGTAATATTTTATCTATTGAAGAA
AAACCAAAAGGTAACTCCGATCAATTAAAGGAAGGCATTGGGTATTCGATATCAGATGAAAGAGCTATATTACCAGAAAA
CCATATTGTTAATGAAGAATTAAAAGAAGAGCTTATCCAAGGTATCAAACTATTGAATGAAAAAGAGCAATTGGTGGTCA
GTCTGTTTTATAATGAAGAACTTACATTTACTGAAATTGGCCAGGTGGTTGGCTTAACCACGTCGAGAATCTCTCAAATT
CACAAACGATCTATCTTTAAATTGAAAAAAACGTTAGAAAAAGTCCAAGCCATGTAG

Upstream 100 bases:

>100_bases
GAATTTGATCCTAGATCAAAATTACTTGCTGTTCGTAAAATAAATCAAGAAGTAATAATGATTTAAATTCAGAATTAAGA
ATAGAAAGGGGAGCTCCAAA

Downstream 100 bases:

>100_bases
TTGCTTGTACATAAGCGATAAAGGGGTAAGAGGGATGAAAGTAGATAATCCGTGGAGAATTTATACGAGTAAAGACAAAA
TGGCAGCCTATATAGAAATA

Product: RNA polymerase sigma factor SigD

Products: NA

Alternate protein names: Sigma-28 [H]

Number of amino acids: Translated: 258; Mature: 258

Protein sequence:

>258_residues
MNMTNSPLEQKLWDKWKNHQDGEAANQLINNYMYLVNYHTERIASHLPNNVVKDDIRSFGILGLYDALKKFDPSRDLKFD
TYASFRIKGSIMDGLRKEDWLPRSIREKTKKVEQAMEEYEQSYQREANSSEIANLTGMTTEEVETTMKDALFSNILSIEE
KPKGNSDQLKEGIGYSISDERAILPENHIVNEELKEELIQGIKLLNEKEQLVVSLFYNEELTFTEIGQVVGLTTSRISQI
HKRSIFKLKKTLEKVQAM

Sequences:

>Translated_258_residues
MNMTNSPLEQKLWDKWKNHQDGEAANQLINNYMYLVNYHTERIASHLPNNVVKDDIRSFGILGLYDALKKFDPSRDLKFD
TYASFRIKGSIMDGLRKEDWLPRSIREKTKKVEQAMEEYEQSYQREANSSEIANLTGMTTEEVETTMKDALFSNILSIEE
KPKGNSDQLKEGIGYSISDERAILPENHIVNEELKEELIQGIKLLNEKEQLVVSLFYNEELTFTEIGQVVGLTTSRISQI
HKRSIFKLKKTLEKVQAM
>Mature_258_residues
MNMTNSPLEQKLWDKWKNHQDGEAANQLINNYMYLVNYHTERIASHLPNNVVKDDIRSFGILGLYDALKKFDPSRDLKFD
TYASFRIKGSIMDGLRKEDWLPRSIREKTKKVEQAMEEYEQSYQREANSSEIANLTGMTTEEVETTMKDALFSNILSIEE
KPKGNSDQLKEGIGYSISDERAILPENHIVNEELKEELIQGIKLLNEKEQLVVSLFYNEELTFTEIGQVVGLTTSRISQI
HKRSIFKLKKTLEKVQAM

Specific function: Sigma factors are initiation factors that promote the attachment of RNA polymerase to specific initiation sites and are then released. This alternative sigma factor is required for the transcription of the flagellin and motility genes as well as for wild-

COG id: COG1191

COG function: function code K; DNA-directed RNA polymerase specialized sigma subunit

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the sigma-70 factor family [H]

Homologues:

Organism=Escherichia coli, GI1788231, Length=228, Percent_Identity=32.4561403508772, Blast_Score=134, Evalue=5e-33,
Organism=Escherichia coli, GI1789098, Length=201, Percent_Identity=25.8706467661692, Blast_Score=64, Evalue=9e-12,

Paralogues:

None

Copy number: 10-20 (rich media) [C]

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR014284
- InterPro:   IPR000943
- InterPro:   IPR007627
- InterPro:   IPR007624
- InterPro:   IPR007630
- InterPro:   IPR012845
- InterPro:   IPR013325
- InterPro:   IPR013324
- InterPro:   IPR011991 [H]

Pfam domain/function: PF04542 Sigma70_r2; PF04539 Sigma70_r3; PF04545 Sigma70_r4 [H]

EC number: NA

Molecular weight: Translated: 29885; Mature: 29885

Theoretical pI: Translated: 5.29; Mature: 5.29

Prosite motif: PS00716 SIGMA70_2

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.0 %Cys     (Translated Protein)
3.1 %Met     (Translated Protein)
3.1 %Cys+Met (Translated Protein)
0.0 %Cys     (Mature Protein)
3.1 %Met     (Mature Protein)
3.1 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MNMTNSPLEQKLWDKWKNHQDGEAANQLINNYMYLVNYHTERIASHLPNNVVKDDIRSFG
CCCCCCHHHHHHHHHHCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHCCCHHHHHHHHHHC
ILGLYDALKKFDPSRDLKFDTYASFRIKGSIMDGLRKEDWLPRSIREKTKKVEQAMEEYE
HHHHHHHHHHCCCCCCCCCCCHHHHEECHHHHHHHHHCCCCCHHHHHHHHHHHHHHHHHH
QSYQREANSSEIANLTGMTTEEVETTMKDALFSNILSIEEKPKGNSDQLKEGIGYSISDE
HHHHHCCCHHHHHHHCCCCHHHHHHHHHHHHHHHHHHHHCCCCCCHHHHHHCCCCCCCCC
RAILPENHIVNEELKEELIQGIKLLNEKEQLVVSLFYNEELTFTEIGQVVGLTTSRISQI
CCCCCCCCCCCHHHHHHHHHHHHHHCHHHHHHHHHHHCCCCCHHHHHHHHHHHHHHHHHH
HKRSIFKLKKTLEKVQAM
HHHHHHHHHHHHHHHHCC
>Mature Secondary Structure
MNMTNSPLEQKLWDKWKNHQDGEAANQLINNYMYLVNYHTERIASHLPNNVVKDDIRSFG
CCCCCCHHHHHHHHHHCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHCCCHHHHHHHHHHC
ILGLYDALKKFDPSRDLKFDTYASFRIKGSIMDGLRKEDWLPRSIREKTKKVEQAMEEYE
HHHHHHHHHHCCCCCCCCCCCHHHHEECHHHHHHHHHCCCCCHHHHHHHHHHHHHHHHHH
QSYQREANSSEIANLTGMTTEEVETTMKDALFSNILSIEEKPKGNSDQLKEGIGYSISDE
HHHHHCCCHHHHHHHCCCCHHHHHHHHHHHHHHHHHHHHCCCCCCHHHHHHCCCCCCCCC
RAILPENHIVNEELKEELIQGIKLLNEKEQLVVSLFYNEELTFTEIGQVVGLTTSRISQI
CCCCCCCCCCCHHHHHHHHHHHHHHCHHHHHHHHHHHCCCCCHHHHHHHHHHHHHHHHHH
HKRSIFKLKKTLEKVQAM
HHHHHHHHHHHHHHHHCC

PDB accession: NA

Resolution: NA

Structure class: Alpha

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: DNA [C]

Specific reaction: Protein + DNA = Protein-DNA [C]

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 2832368; 9384377; 7602586; 8157612 [H]