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The map label for this gene is cheB

Identifier: 23099033

GI number: 23099033

Start: 1616722

End: 1617780

Strand: Direct

Name: cheB

Synonym: OB1578

Alternate gene names: 23099033

Gene position: 1616722-1617780 (Clockwise)

Preceding gene: 23099032

Following gene: 23099034

Centisome position: 44.53

GC content: 37.96

Gene sequence:

>1059_bases
ATGAGAAAAGTTCGGGCAATAGTAATTGATGACTCCGCATTTATGAGGAAAATAATTTCCGATATTTTAGATAACGACCC
AAGAATTGAAGTGGTAGCTGTTGCCCGAAACGGTGAAGATGGACTAAATAAAGTCATACAGCTATCACCGGATGTGGTAA
CCCTTGATGTGCATATGCCAAAAATGGATGGCATGCAAGCATTACAACGAATTATGAATGAACACCCCGTTCCTGTAGTA
ATGTTATCCAGTGTTACGAAAGCAGGAGCAGAAAAAACAATTCAAGCCATTTCAAATGGCGCTGTAGACTTTATCATGAA
GCCCTCAGGTTCGATTTCATTAGATATTCGAAATGTAGAGGACGAAATTCGAAAAAAGGTTATATTGGCTTCTACGGTTA
GGGTGAAATCTACCCAAGAAGCGTCCGAGGAAGATTTTCAATCGACAAGGACCGTTCCTACTATAAATAGAGAAAAAAAG
TATCGAAGAAGTATAGTTTCTATCGGTACTTCTACGGGCGGACCGAAAGCATTACAAAAGGTTTTAACAGAATTGCCTAA
AGATATACAAGCTCCAATCGTTATTGTACAGCATATGCCACCTGGCTTTACAAAATCATTAGCTGATCGATTAAATTCCA
TTTGTGCCATTTCAGTTAAAGAAGCGGTTCACGGAGAAATATTGCAATCTGGTACTGCTTATATTGCACCAGGAGGTTTT
CATATGCAGGTGGAGAATGTAGGTATGTCCTTAGCAATAGCGTTAGATAAATCTCCTCCATTAAAAGGGCATCGGCCATC
TGTAAATAAACTTTTTAGTTCTCTTCAACATATTAAAAATTACAATAAGATTACGTGCATTTTAACGGGAATGGGAAACG
ATGGAACGGATGGTCTACAGCAATTAAGGTTAAATGAGGGGTCTACGATATCTTTGGCAGAATCAGCAGATACTGCCATT
GTATATGGAATGCCAAAAGCTGCAGTAAATGCAGGGTTAATCGATTATGAAATGTCTTTACATGAAATACCTAGTTTGAT
AGTAAAACAATTAACATAA

Upstream 100 bases:

>100_bases
ACACAACTAGCAGAGGTTTACTTAAAAGAAAGAAATATGATTGATTCCGTAAATACAAGCTTTCTAGGTAGGTTAAAGAA
CTGGATGGAAGGGTGACCTT

Downstream 100 bases:

>100_bases
AAATTTTAGGAGGTACTGGTCATGGTACAAGAAATGATAAAGAGCCAAAAAATGATTGTTTTTGATTTAGAAGGTGGGGA
ATATGCACTTCCAGTTGAGT

Product: chemotaxis-specific methylesterase

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 352; Mature: 352

Protein sequence:

>352_residues
MRKVRAIVIDDSAFMRKIISDILDNDPRIEVVAVARNGEDGLNKVIQLSPDVVTLDVHMPKMDGMQALQRIMNEHPVPVV
MLSSVTKAGAEKTIQAISNGAVDFIMKPSGSISLDIRNVEDEIRKKVILASTVRVKSTQEASEEDFQSTRTVPTINREKK
YRRSIVSIGTSTGGPKALQKVLTELPKDIQAPIVIVQHMPPGFTKSLADRLNSICAISVKEAVHGEILQSGTAYIAPGGF
HMQVENVGMSLAIALDKSPPLKGHRPSVNKLFSSLQHIKNYNKITCILTGMGNDGTDGLQQLRLNEGSTISLAESADTAI
VYGMPKAAVNAGLIDYEMSLHEIPSLIVKQLT

Sequences:

>Translated_352_residues
MRKVRAIVIDDSAFMRKIISDILDNDPRIEVVAVARNGEDGLNKVIQLSPDVVTLDVHMPKMDGMQALQRIMNEHPVPVV
MLSSVTKAGAEKTIQAISNGAVDFIMKPSGSISLDIRNVEDEIRKKVILASTVRVKSTQEASEEDFQSTRTVPTINREKK
YRRSIVSIGTSTGGPKALQKVLTELPKDIQAPIVIVQHMPPGFTKSLADRLNSICAISVKEAVHGEILQSGTAYIAPGGF
HMQVENVGMSLAIALDKSPPLKGHRPSVNKLFSSLQHIKNYNKITCILTGMGNDGTDGLQQLRLNEGSTISLAESADTAI
VYGMPKAAVNAGLIDYEMSLHEIPSLIVKQLT
>Mature_352_residues
MRKVRAIVIDDSAFMRKIISDILDNDPRIEVVAVARNGEDGLNKVIQLSPDVVTLDVHMPKMDGMQALQRIMNEHPVPVV
MLSSVTKAGAEKTIQAISNGAVDFIMKPSGSISLDIRNVEDEIRKKVILASTVRVKSTQEASEEDFQSTRTVPTINREKK
YRRSIVSIGTSTGGPKALQKVLTELPKDIQAPIVIVQHMPPGFTKSLADRLNSICAISVKEAVHGEILQSGTAYIAPGGF
HMQVENVGMSLAIALDKSPPLKGHRPSVNKLFSSLQHIKNYNKITCILTGMGNDGTDGLQQLRLNEGSTISLAESADTAI
VYGMPKAAVNAGLIDYEMSLHEIPSLIVKQLT

Specific function: Involved in the modulation of the chemotaxis system; catalyzes the demethylation of specific methylglutamate residues introduced into the chemoreceptors (methyl-accepting chemotaxis proteins) by CheR

COG id: COG2201

COG function: function code NT; Chemotaxis response regulator containing a CheY-like receiver domain and a methylesterase domain

Gene ontology:

Cell location: Cytoplasm

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 response regulatory domain

Homologues:

Organism=Escherichia coli, GI1788192, Length=332, Percent_Identity=39.7590361445783, Blast_Score=244, Evalue=7e-66,
Organism=Escherichia coli, GI87082052, Length=160, Percent_Identity=26.875, Blast_Score=65, Evalue=5e-12,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): CHEB_OCEIH (Q8EQW0)

Other databases:

- EMBL:   BA000028
- RefSeq:   NP_692499.1
- ProteinModelPortal:   Q8EQW0
- SMR:   Q8EQW0
- GeneID:   1017966
- GenomeReviews:   BA000028_GR
- KEGG:   oih:OB1578
- NMPDR:   fig|221109.1.peg.1580
- HOGENOM:   HBG705324
- OMA:   SALERIM
- ProtClustDB:   PRK00742
- BioCyc:   OIHE221109:OB1578-MONOMER
- BRENDA:   3.1.1.61
- GO:   GO:0005737
- GO:   GO:0006935
- HAMAP:   MF_00099
- InterPro:   IPR011006
- InterPro:   IPR008248
- InterPro:   IPR000673
- InterPro:   IPR001789
- Gene3D:   G3DSA:3.40.50.180
- PIRSF:   PIRSF000876
- SMART:   SM00448

Pfam domain/function: PF01339 CheB_methylest; PF00072 Response_reg; SSF52738 Chemotax_RR_pGlu_Me-esterase; SSF52172 CheY_like

EC number: =3.1.1.61

Molecular weight: Translated: 38223; Mature: 38223

Theoretical pI: Translated: 8.66; Mature: 8.66

Prosite motif: PS50122 CHEB; PS50110 RESPONSE_REGULATORY

Important sites: ACT_SITE 171-171 ACT_SITE 198-198 ACT_SITE 294-294

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.6 %Cys     (Translated Protein)
4.0 %Met     (Translated Protein)
4.5 %Cys+Met (Translated Protein)
0.6 %Cys     (Mature Protein)
4.0 %Met     (Mature Protein)
4.5 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MRKVRAIVIDDSAFMRKIISDILDNDPRIEVVAVARNGEDGLNKVIQLSPDVVTLDVHMP
CCCEEEEEECCHHHHHHHHHHHHCCCCCEEEEEEEECCCHHHHHHEECCCCEEEEEEECC
KMDGMQALQRIMNEHPVPVVMLSSVTKAGAEKTIQAISNGAVDFIMKPSGSISLDIRNVE
CCCHHHHHHHHHHCCCCCEEEEHHHHHHHHHHHHHHHHCCCEEEEECCCCCEEEEEECHH
DEIRKKVILASTVRVKSTQEASEEDFQSTRTVPTINREKKYRRSIVSIGTSTGGPKALQK
HHHHHHHHHHHHEEECCCHHHHHHHHHHHCCCCCCCHHHHHHHHHHHHCCCCCCHHHHHH
VLTELPKDIQAPIVIVQHMPPGFTKSLADRLNSICAISVKEAVHGEILQSGTAYIAPGGF
HHHHCCCCCCCCEEEEECCCCCHHHHHHHHHHHHEEEEHHHHHHHHHHHCCCEEEECCCE
HMQVENVGMSLAIALDKSPPLKGHRPSVNKLFSSLQHIKNYNKITCILTGMGNDGTDGLQ
EEEEECCCEEEEEEECCCCCCCCCCCCHHHHHHHHHHHCCCCEEEEEEEECCCCCHHHHH
QLRLNEGSTISLAESADTAIVYGMPKAAVNAGLIDYEMSLHEIPSLIVKQLT
HHHCCCCCEEEEECCCCCEEEEECCHHHHHCCEEEEHHHHHHHHHHHHHHCC
>Mature Secondary Structure
MRKVRAIVIDDSAFMRKIISDILDNDPRIEVVAVARNGEDGLNKVIQLSPDVVTLDVHMP
CCCEEEEEECCHHHHHHHHHHHHCCCCCEEEEEEEECCCHHHHHHEECCCCEEEEEEECC
KMDGMQALQRIMNEHPVPVVMLSSVTKAGAEKTIQAISNGAVDFIMKPSGSISLDIRNVE
CCCHHHHHHHHHHCCCCCEEEEHHHHHHHHHHHHHHHHCCCEEEEECCCCCEEEEEECHH
DEIRKKVILASTVRVKSTQEASEEDFQSTRTVPTINREKKYRRSIVSIGTSTGGPKALQK
HHHHHHHHHHHHEEECCCHHHHHHHHHHHCCCCCCCHHHHHHHHHHHHCCCCCCHHHHHH
VLTELPKDIQAPIVIVQHMPPGFTKSLADRLNSICAISVKEAVHGEILQSGTAYIAPGGF
HHHHCCCCCCCCEEEEECCCCCHHHHHHHHHHHHEEEEHHHHHHHHHHHCCCEEEECCCE
HMQVENVGMSLAIALDKSPPLKGHRPSVNKLFSSLQHIKNYNKITCILTGMGNDGTDGLQ
EEEEECCCEEEEEEECCCCCCCCCCCCHHHHHHHHHHHCCCCEEEEEEEECCCCCHHHHH
QLRLNEGSTISLAESADTAIVYGMPKAAVNAGLIDYEMSLHEIPSLIVKQLT
HHHCCCCCEEEEECCCCCEEEEECCHHHHHCCEEEEHHHHHHHHHHHHHHCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 12235376