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The map label for this gene is fliM [H]

Identifier: 23099022

GI number: 23099022

Start: 1606683

End: 1607675

Strand: Direct

Name: fliM [H]

Synonym: OB1567

Alternate gene names: 23099022

Gene position: 1606683-1607675 (Clockwise)

Preceding gene: 23099021

Following gene: 23099023

Centisome position: 44.25

GC content: 34.84

Gene sequence:

>993_bases
ATGGATGAAGTTTTATCGCAGAATGAAATTGACGCTTTATTATCTGCTATCTCTTCTGGAGAGATGGATGCTGATGAGTT
AAAAAAAGAAGAAGAAGAGAAAAAGATACGAGTTTATGATTTTAAACGTGCGCTTCGTTTTTCAAAAGATCAAATACGAA
GTATCTCTCGAATACACGAGAATTATGCTAGAATGCTTACAACTTTCTTTGCTACTCAACTACGCAGCTATGTTAATATC
TCAGTTACATCCGTTGATCAAGTACCATATGAAGAATTTATTCGCTCCATTCCAAAGAAAACAATACTTAATCTATATGG
TATGGAACCGTTAGATGGTAATTTGATTATGGAAATCAATCCTAACATTGCTTATGCGTTACTTGACCGTTTACTTGGCG
GAAAGGGAAGTACCATTACAAAAGATGGTAATCTAACAGAGATTGAGAAAACATTATTTATGCAGATGTTTGAAAAAGCA
GAAGATGCATTAAGAGATGCCTGGAGTTCAGTGGTTGAAATTGAGCCAGTATTAGAAGAATTTGAAGAAAATCCGCAATT
TATGCAAAAGATTGCACCTAATGAAACCGTGGTCGTCGTATCTCTTACTACGGTTATCGGAGAAACAACGGGAATGATTA
ATATTTGTATTCCTCATGTAATTTTAGAACCGATTATTCCAAAGCTGTCAGTACATTACTGGATGCAATCTAATTCAAAT
AAAGATGAACAAGCTTATGGCAAGATGCAGCAGAATTTGAAGAAGACAGAGGTTGAATTAAAGGCTATTCTTGGGGCGAC
AACAATATCAATTCATGATTTTCTCAACTTTCAACAAGGAGACATCATTTCGCTTGATCAACCAATTGATGCACCATTGC
TGTTATCGATATATGATCAACCGAAGTTCTTTGCACAACCAGGAAAATTCAAAAATAAGATGTCTGTTCAAATTTTAGAA
GAAATTAAAGGGGTGAATGAAGATGATGAATGA

Upstream 100 bases:

>100_bases
ATTAAATAACTTAATGACAGAAGGAAACGTTACTGAAGTATATACGATAAGTAAGATCCTTCAATAGAAGCTAGCTCCTG
CTAGGAGGTGAACGTAATAC

Downstream 100 bases:

>100_bases
TGGAATGCTTTCTCAGGATGAAATTGATGCATTACTTAAGGTATCTGAGGATGATTCTGAAGAAATATCAAATGAAGACA
CATCACAAGCCTATTTATCT

Product: flagellar motor switch protein FliM

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 330; Mature: 330

Protein sequence:

>330_residues
MDEVLSQNEIDALLSAISSGEMDADELKKEEEEKKIRVYDFKRALRFSKDQIRSISRIHENYARMLTTFFATQLRSYVNI
SVTSVDQVPYEEFIRSIPKKTILNLYGMEPLDGNLIMEINPNIAYALLDRLLGGKGSTITKDGNLTEIEKTLFMQMFEKA
EDALRDAWSSVVEIEPVLEEFEENPQFMQKIAPNETVVVVSLTTVIGETTGMINICIPHVILEPIIPKLSVHYWMQSNSN
KDEQAYGKMQQNLKKTEVELKAILGATTISIHDFLNFQQGDIISLDQPIDAPLLLSIYDQPKFFAQPGKFKNKMSVQILE
EIKGVNEDDE

Sequences:

>Translated_330_residues
MDEVLSQNEIDALLSAISSGEMDADELKKEEEEKKIRVYDFKRALRFSKDQIRSISRIHENYARMLTTFFATQLRSYVNI
SVTSVDQVPYEEFIRSIPKKTILNLYGMEPLDGNLIMEINPNIAYALLDRLLGGKGSTITKDGNLTEIEKTLFMQMFEKA
EDALRDAWSSVVEIEPVLEEFEENPQFMQKIAPNETVVVVSLTTVIGETTGMINICIPHVILEPIIPKLSVHYWMQSNSN
KDEQAYGKMQQNLKKTEVELKAILGATTISIHDFLNFQQGDIISLDQPIDAPLLLSIYDQPKFFAQPGKFKNKMSVQILE
EIKGVNEDDE
>Mature_330_residues
MDEVLSQNEIDALLSAISSGEMDADELKKEEEEKKIRVYDFKRALRFSKDQIRSISRIHENYARMLTTFFATQLRSYVNI
SVTSVDQVPYEEFIRSIPKKTILNLYGMEPLDGNLIMEINPNIAYALLDRLLGGKGSTITKDGNLTEIEKTLFMQMFEKA
EDALRDAWSSVVEIEPVLEEFEENPQFMQKIAPNETVVVVSLTTVIGETTGMINICIPHVILEPIIPKLSVHYWMQSNSN
KDEQAYGKMQQNLKKTEVELKAILGATTISIHDFLNFQQGDIISLDQPIDAPLLLSIYDQPKFFAQPGKFKNKMSVQILE
EIKGVNEDDE

Specific function: One of the proteins that form a switch complex that is proposed to be located at the base of the basal body. This complex interacts with chemotaxis proteins (such as CheY) in addition to contacting components of the motor that determine the direction of f

COG id: COG1868

COG function: function code N; Flagellar motor switch protein

Gene ontology:

Cell location: Cell membrane; Peripheral membrane protein. Bacterial flagellum basal body [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the FliM family [H]

Homologues:

Organism=Escherichia coli, GI1788255, Length=292, Percent_Identity=29.1095890410959, Blast_Score=127, Evalue=9e-31,

Paralogues:

None

Copy number: 10-20 (rich media) [C]

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR001689
- InterPro:   IPR001543 [H]

Pfam domain/function: PF02154 FliM; PF01052 SpoA [H]

EC number: NA

Molecular weight: Translated: 37592; Mature: 37592

Theoretical pI: Translated: 4.44; Mature: 4.44

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.3 %Cys     (Translated Protein)
3.6 %Met     (Translated Protein)
3.9 %Cys+Met (Translated Protein)
0.3 %Cys     (Mature Protein)
3.6 %Met     (Mature Protein)
3.9 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MDEVLSQNEIDALLSAISSGEMDADELKKEEEEKKIRVYDFKRALRFSKDQIRSISRIHE
CCCCCCHHHHHHHHHHHHCCCCCHHHHHHHHHHHHHHHHHHHHHHHCCHHHHHHHHHHHH
NYARMLTTFFATQLRSYVNISVTSVDQVPYEEFIRSIPKKTILNLYGMEPLDGNLIMEIN
HHHHHHHHHHHHHHHHHHEEEEECCCCCCHHHHHHHCCHHHHHHHHCCCCCCCCEEEEEC
PNIAYALLDRLLGGKGSTITKDGNLTEIEKTLFMQMFEKAEDALRDAWSSVVEIEPVLEE
CCHHHHHHHHHHCCCCCEEECCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
FEENPQFMQKIAPNETVVVVSLTTVIGETTGMINICIPHVILEPIIPKLSVHYWMQSNSN
HHCCHHHHHHCCCCCEEEEEEHHHHHCCCCCCHHHHHHHHHHHHHHHHHHHHEEECCCCC
KDEQAYGKMQQNLKKTEVELKAILGATTISIHDFLNFQQGDIISLDQPIDAPLLLSIYDQ
CHHHHHHHHHHHHHHHHHHHHHHHHHHHEEHHHHHCCCCCCEEECCCCCCCCEEEEECCC
PKFFAQPGKFKNKMSVQILEEIKGVNEDDE
CCHHCCCCCCCHHHHHHHHHHHCCCCCCCC
>Mature Secondary Structure
MDEVLSQNEIDALLSAISSGEMDADELKKEEEEKKIRVYDFKRALRFSKDQIRSISRIHE
CCCCCCHHHHHHHHHHHHCCCCCHHHHHHHHHHHHHHHHHHHHHHHCCHHHHHHHHHHHH
NYARMLTTFFATQLRSYVNISVTSVDQVPYEEFIRSIPKKTILNLYGMEPLDGNLIMEIN
HHHHHHHHHHHHHHHHHHEEEEECCCCCCHHHHHHHCCHHHHHHHHCCCCCCCCEEEEEC
PNIAYALLDRLLGGKGSTITKDGNLTEIEKTLFMQMFEKAEDALRDAWSSVVEIEPVLEE
CCHHHHHHHHHHCCCCCEEECCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
FEENPQFMQKIAPNETVVVVSLTTVIGETTGMINICIPHVILEPIIPKLSVHYWMQSNSN
HHCCHHHHHHCCCCCEEEEEEHHHHHCCCCCCHHHHHHHHHHHHHHHHHHHHEEECCCCC
KDEQAYGKMQQNLKKTEVELKAILGATTISIHDFLNFQQGDIISLDQPIDAPLLLSIYDQ
CHHHHHHHHHHHHHHHHHHHHHHHHHHHEEHHHHHCCCCCCEEECCCCCCCCEEEEECCC
PKFFAQPGKFKNKMSVQILEEIKGVNEDDE
CCHHCCCCCCCHHHHHHHHHHHCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 7.0

TargetDB status: NA

Availability: NA

References: 1898932; 9384377; 1828465; 1447979 [H]