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The map label for this gene is fliF [H]

Identifier: 23099010

GI number: 23099010

Start: 1597115

End: 1598713

Strand: Direct

Name: fliF [H]

Synonym: OB1555

Alternate gene names: 23099010

Gene position: 1597115-1598713 (Clockwise)

Preceding gene: 23099009

Following gene: 23099011

Centisome position: 43.99

GC content: 36.65

Gene sequence:

>1599_bases
ATGAAGGAAAAATTGTTGAAAGGGAAGGATTCTGTCTCGTCATTTTGGGCTTCTCGTTCCAAGGTTCAAAAGGGAAGTGT
CATTGGTGGGTTTCTTCTAATCATTTTACTAATAGGTGTAATTACCTTTGCATCGACAAACACGAACTATGTACCACTCT
ATAACAATTTATCAACACAGGAAGCTGGCCAAATTACAACTGAGTTAGAATCTCGAGGCGTTCCTTTTGAATTAGAAAAT
GGAAGGTCAACCATTACAGTTCCTGAAGAACAAGTTGATACATTATTACTAGATTTAGCGTCCATGGGACTTCCGAATAA
TGGTAGTATTGATTATTCGTTTTTTAGTGAGAACACCTCTTGGGGAATTACAGATAATGAGTTTAATGTAATGAAGTTAG
ATGCTATGCAGACAGAGCTTGCCAATTTAATTAAAAGTGTTGAAGGAATTCAAGATGCACAAGTCATGATCAATGTACCA
GAAGAACCTGTATTTGCAACGGATGTTACCGGTGAGGCAAGTGCTTCAATTGTGCTTAACACTCAACCGGGATATCAGTT
TGAAGGGAATCAAATAGAAGGACTTCATCATTTAGTTGCAAAAGCAGTTCCAAATTTACCGGAAGAAAATATTGTAATTA
TGAATCAATACTTTGAGTATTTTGATCAAAATACTCAACAAACTGCAAATCAAGATACATATACACATCAACAAACAGTA
AAAAAAGAAGTGGAAAGAGACATACAACGTAGATTGCAACAAATGCTCGGTACGATGGTGGGCAATGAAAACATTGTAGT
ATCCGTTACAGCAGACATAGATTTCACACAAGAAAATCGTAAAGAAGAATTAGTAGAACCAGTGGACATAGAAAATATGG
AAGGTTTACCTGTTAGTTTAGAAACGATTCAAGAATCTTATGTCGGAGAAGGTGCCGCAGAAGCTGGGACAAATGAGGAA
GTAGGGACTGGTGAAGGAGATGTGCCTGGATATAATTCTGGGGATAATGGAGACGGAGAATACGAATTAGATAAAGAAAC
GATTAATTATGAATTTAATCGGATTCGTCGTGATATTGTAGAAAGTCCTTACAAGCTTCGAGATCTAGGTATTCAAGTGG
CTGTTAATAATACGAGAAGCGTAGACAATGAATTGGAGTATCTCTCGCAACAAGAGCAGACATCAGTGGAGGAAGGAATT
AATTCTATTTTAAATTCGATTATAACTTCGTCTATCGATGGGGAGTACAACCAAGACTTAGTACCTGATGATAAAACCTC
GATCGTATTTCAAGAATTTACAGAGACAACCACACCAAGCGCAGAAGGAACAACTGGTATTCCAACTTGGTTGTATGTTG
TAGCAGGAATATTACTACTTATTATAATTGTATTGTTGTTCTTCTTAATAAGAAACCGAAAAACATCGGATGAGGTAGTC
GATCAGTATGAAGAAATTAATGAACAAACACCAATACCGGACTTGCCTGAAAAAGAAGAATCAGAAGCTACAATCAGAAG
AAAACAATTAGAGAAAATAGCGAAAGAAAAACCAGAGGACTTTGCAAAATTATTGCGTAGCTGGATTGGTGAAGATTAG

Upstream 100 bases:

>100_bases
TTGACGCATATAACGAAATAATGAGGATGCAAGTATAGCAATAGAATTTATTCATATTCATAATAGTTTAAACGTATGTG
CGTTGTAGTGGGGGAAATAT

Downstream 100 bases:

>100_bases
GAGGATGTGAAGTGGTTAGAACAAAAGGTGCGTTAACAGGAAAACAAAAAGCAGCAATTCTTCTAATATCTTTAGGACCA
GACGTCTCTGCACAAGTATA

Product: flagellar MS-ring protein

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 532; Mature: 532

Protein sequence:

>532_residues
MKEKLLKGKDSVSSFWASRSKVQKGSVIGGFLLIILLIGVITFASTNTNYVPLYNNLSTQEAGQITTELESRGVPFELEN
GRSTITVPEEQVDTLLLDLASMGLPNNGSIDYSFFSENTSWGITDNEFNVMKLDAMQTELANLIKSVEGIQDAQVMINVP
EEPVFATDVTGEASASIVLNTQPGYQFEGNQIEGLHHLVAKAVPNLPEENIVIMNQYFEYFDQNTQQTANQDTYTHQQTV
KKEVERDIQRRLQQMLGTMVGNENIVVSVTADIDFTQENRKEELVEPVDIENMEGLPVSLETIQESYVGEGAAEAGTNEE
VGTGEGDVPGYNSGDNGDGEYELDKETINYEFNRIRRDIVESPYKLRDLGIQVAVNNTRSVDNELEYLSQQEQTSVEEGI
NSILNSIITSSIDGEYNQDLVPDDKTSIVFQEFTETTTPSAEGTTGIPTWLYVVAGILLLIIIVLLFFLIRNRKTSDEVV
DQYEEINEQTPIPDLPEKEESEATIRRKQLEKIAKEKPEDFAKLLRSWIGED

Sequences:

>Translated_532_residues
MKEKLLKGKDSVSSFWASRSKVQKGSVIGGFLLIILLIGVITFASTNTNYVPLYNNLSTQEAGQITTELESRGVPFELEN
GRSTITVPEEQVDTLLLDLASMGLPNNGSIDYSFFSENTSWGITDNEFNVMKLDAMQTELANLIKSVEGIQDAQVMINVP
EEPVFATDVTGEASASIVLNTQPGYQFEGNQIEGLHHLVAKAVPNLPEENIVIMNQYFEYFDQNTQQTANQDTYTHQQTV
KKEVERDIQRRLQQMLGTMVGNENIVVSVTADIDFTQENRKEELVEPVDIENMEGLPVSLETIQESYVGEGAAEAGTNEE
VGTGEGDVPGYNSGDNGDGEYELDKETINYEFNRIRRDIVESPYKLRDLGIQVAVNNTRSVDNELEYLSQQEQTSVEEGI
NSILNSIITSSIDGEYNQDLVPDDKTSIVFQEFTETTTPSAEGTTGIPTWLYVVAGILLLIIIVLLFFLIRNRKTSDEVV
DQYEEINEQTPIPDLPEKEESEATIRRKQLEKIAKEKPEDFAKLLRSWIGED
>Mature_532_residues
MKEKLLKGKDSVSSFWASRSKVQKGSVIGGFLLIILLIGVITFASTNTNYVPLYNNLSTQEAGQITTELESRGVPFELEN
GRSTITVPEEQVDTLLLDLASMGLPNNGSIDYSFFSENTSWGITDNEFNVMKLDAMQTELANLIKSVEGIQDAQVMINVP
EEPVFATDVTGEASASIVLNTQPGYQFEGNQIEGLHHLVAKAVPNLPEENIVIMNQYFEYFDQNTQQTANQDTYTHQQTV
KKEVERDIQRRLQQMLGTMVGNENIVVSVTADIDFTQENRKEELVEPVDIENMEGLPVSLETIQESYVGEGAAEAGTNEE
VGTGEGDVPGYNSGDNGDGEYELDKETINYEFNRIRRDIVESPYKLRDLGIQVAVNNTRSVDNELEYLSQQEQTSVEEGI
NSILNSIITSSIDGEYNQDLVPDDKTSIVFQEFTETTTPSAEGTTGIPTWLYVVAGILLLIIIVLLFFLIRNRKTSDEVV
DQYEEINEQTPIPDLPEKEESEATIRRKQLEKIAKEKPEDFAKLLRSWIGED

Specific function: The M ring may be actively involved in energy transduction [H]

COG id: COG1766

COG function: function code NU; Flagellar biosynthesis/type III secretory pathway lipoprotein

Gene ontology:

Cell location: Cell membrane; Multi-pass membrane protein. Bacterial flagellum basal body (By similarity) [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the fliF family [H]

Homologues:

Organism=Escherichia coli, GI1788248, Length=305, Percent_Identity=28.1967213114754, Blast_Score=130, Evalue=2e-31,

Paralogues:

None

Copy number: 10-20 (rich media) [C]

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR013556
- InterPro:   IPR000067
- InterPro:   IPR006182 [H]

Pfam domain/function: PF01514 YscJ_FliF; PF08345 YscJ_FliF_C [H]

EC number: NA

Molecular weight: Translated: 59461; Mature: 59461

Theoretical pI: Translated: 3.96; Mature: 3.96

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.0 %Cys     (Translated Protein)
1.7 %Met     (Translated Protein)
1.7 %Cys+Met (Translated Protein)
0.0 %Cys     (Mature Protein)
1.7 %Met     (Mature Protein)
1.7 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MKEKLLKGKDSVSSFWASRSKVQKGSVIGGFLLIILLIGVITFASTNTNYVPLYNNLSTQ
CCCCHHCCCHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHCCCCCCEEEEECCCCCH
EAGQITTELESRGVPFELENGRSTITVPEEQVDTLLLDLASMGLPNNGSIDYSFFSENTS
HHHHHHHHHHHCCCCEEECCCCEEEECCHHHHHHHHHHHHHCCCCCCCCEEEEEECCCCC
WGITDNEFNVMKLDAMQTELANLIKSVEGIQDAQVMINVPEEPVFATDVTGEASASIVLN
CCCCCCCCCEEEHHHHHHHHHHHHHHHHCCCCCEEEEECCCCCEEEECCCCCCCCEEEEE
TQPGYQFEGNQIEGLHHLVAKAVPNLPEENIVIMNQYFEYFDQNTQQTANQDTYTHQQTV
CCCCCEECCCHHHHHHHHHHHHCCCCCCCCEEEHHHHHHHHCCCHHHHCCCCHHHHHHHH
KKEVERDIQRRLQQMLGTMVGNENIVVSVTADIDFTQENRKEELVEPVDIENMEGLPVSL
HHHHHHHHHHHHHHHHHHHHCCCCEEEEEEECCCCCHHCCHHHCCCCCCCCCCCCCCCHH
ETIQESYVGEGAAEAGTNEEVGTGEGDVPGYNSGDNGDGEYELDKETINYEFNRIRRDIV
HHHHHHHCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCEEECHHHHHHHHHHHHHHHH
ESPYKLRDLGIQVAVNNTRSVDNELEYLSQQEQTSVEEGINSILNSIITSSIDGEYNQDL
HCCHHHHHCCEEEEECCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCC
VPDDKTSIVFQEFTETTTPSAEGTTGIPTWLYVVAGILLLIIIVLLFFLIRNRKTSDEVV
CCCCCHHHHHHHHHCCCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHCCCCHHHHH
DQYEEINEQTPIPDLPEKEESEATIRRKQLEKIAKEKPEDFAKLLRSWIGED
HHHHHHHCCCCCCCCCCCCHHHHHHHHHHHHHHHHCCCHHHHHHHHHHCCCC
>Mature Secondary Structure
MKEKLLKGKDSVSSFWASRSKVQKGSVIGGFLLIILLIGVITFASTNTNYVPLYNNLSTQ
CCCCHHCCCHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHCCCCCCEEEEECCCCCH
EAGQITTELESRGVPFELENGRSTITVPEEQVDTLLLDLASMGLPNNGSIDYSFFSENTS
HHHHHHHHHHHCCCCEEECCCCEEEECCHHHHHHHHHHHHHCCCCCCCCEEEEEECCCCC
WGITDNEFNVMKLDAMQTELANLIKSVEGIQDAQVMINVPEEPVFATDVTGEASASIVLN
CCCCCCCCCEEEHHHHHHHHHHHHHHHHCCCCCEEEEECCCCCEEEECCCCCCCCEEEEE
TQPGYQFEGNQIEGLHHLVAKAVPNLPEENIVIMNQYFEYFDQNTQQTANQDTYTHQQTV
CCCCCEECCCHHHHHHHHHHHHCCCCCCCCEEEHHHHHHHHCCCHHHHCCCCHHHHHHHH
KKEVERDIQRRLQQMLGTMVGNENIVVSVTADIDFTQENRKEELVEPVDIENMEGLPVSL
HHHHHHHHHHHHHHHHHHHHCCCCEEEEEEECCCCCHHCCHHHCCCCCCCCCCCCCCCHH
ETIQESYVGEGAAEAGTNEEVGTGEGDVPGYNSGDNGDGEYELDKETINYEFNRIRRDIV
HHHHHHHCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCEEECHHHHHHHHHHHHHHHH
ESPYKLRDLGIQVAVNNTRSVDNELEYLSQQEQTSVEEGINSILNSIITSSIDGEYNQDL
HCCHHHHHCCEEEEECCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCC
VPDDKTSIVFQEFTETTTPSAEGTTGIPTWLYVVAGILLLIIIVLLFFLIRNRKTSDEVV
CCCCCHHHHHHHHHCCCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHCCCCHHHHH
DQYEEINEQTPIPDLPEKEESEATIRRKQLEKIAKEKPEDFAKLLRSWIGED
HHHHHHHCCCCCCCCCCCCHHHHHHHHHHHHHHHHCCCHHHHHHHHHHCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 6.0

TargetDB status: NA

Availability: NA

References: 1905667; 9384377; 1828465 [H]