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The map label for this gene is pyrAA

Identifier: 23098945

GI number: 23098945

Start: 1530043

End: 1531143

Strand: Direct

Name: pyrAA

Synonym: OB1490

Alternate gene names: 23098945

Gene position: 1530043-1531143 (Clockwise)

Preceding gene: 23098944

Following gene: 23098946

Centisome position: 42.14

GC content: 39.24

Gene sequence:

>1101_bases
ATGAAGAAGCGTAAATTAATACTAGAAGATGGAACTGTTTTTAATGGGACAGCGTTTGGAAGTGATGCCGAATCAAGTGG
TGAGATTGTATTTAATACTGGAATGACAGGTTATCAAGAAGTCATCACAGATCCTAGTTATTGTGGTCAATTCGTTACAT
TAACTTATCCTTTAATTGGAAATTATGGCATTAATCGCGATGATTTTGAAACAGTGACACCATTTATTCATGGTCTTGTT
GTAAAAGAGTATAGTGAATTTCCATCTAACTTTCGAAATGAGGAGACTTTAGATGAGTTTTTACAAGCTCATAATATTCC
TGGAATTGCAAATATTGATACACGGAAGCTCACTCGAATCATACGTAAGCACGGTACAATGCGAGCAGTAATGGTCGATG
AGCAGAAAAATGAGCAACACGTTATAGAACAATTAAGACTTGCTGAAATGCCTCGAGACCAGGTGAAACGAACTTCGACC
ATTAAACCATATGTTGTACCAGGAAGAGGGCTACGAGTAGTAATGGTTGATTTCGGTGCAAAGCATGGGATTCTAAGAGA
ATTAACAAGACGTGACTGTCATATCACCGTTGTCCCTCATAACTATAGTGCGGAAGCAATCTTACGATTAAAACCAGATG
GAATTATGTTGACGAATGGACCTGGGGATCCAAAAGATGTTCCAGAAGCAATTGAAATGATAAAACAACTTCTCGGCCAA
ATCCCTATATTCGGCATTTGCCTTGGACATCAATTGCTGGCATTGGCTTGTGGAGCAGATACGGAAAAAATGAAATTCGG
TCACCGGGGAGCGAATCATCCAGTAAAGGATTTACTAGCCGGAAAAACGTATTTAACATCACAAAACCATAGCTATGCAG
TTAACGTTTCGTCGCTAGTTAACACAGATCTCGAATTAACGCAGATCGCTTTAAATGATGATACGGTAGAAGGAATTCGC
CATACTGCCTTCCCGGCATTTTCAGTTCAATATCATCCAGAAGCATCACCAGGACCGGAGGATACGAATTTCTTATTTGA
TGAGTTTTTAAACCTGATTAAGGCTAGCAAAGTGAAACAAGGAGGAGAAGTATATGCCTAA

Upstream 100 bases:

>100_bases
TCAAAAGGTAAAAACACACCTTTCGATAATTGGAAAGTAAAAGGAATTCCTGTACTTACAATGGTAAATGGAGTAGTTGT
ATACGAGGAGGCTAAACAAC

Downstream 100 bases:

>100_bases
ACGTACAGACATCAACAAAATTCTTGTAATCGGATCAGGACCAATCATTATTGGTCAAGCTGCGGAATTTGATTACTCAG
GTACGCAAGCTTGTCACGCT

Product: carbamoyl phosphate synthase small subunit

Products: NA

Alternate protein names: Carbamoyl-phosphate synthetase glutamine chain

Number of amino acids: Translated: 366; Mature: 366

Protein sequence:

>366_residues
MKKRKLILEDGTVFNGTAFGSDAESSGEIVFNTGMTGYQEVITDPSYCGQFVTLTYPLIGNYGINRDDFETVTPFIHGLV
VKEYSEFPSNFRNEETLDEFLQAHNIPGIANIDTRKLTRIIRKHGTMRAVMVDEQKNEQHVIEQLRLAEMPRDQVKRTST
IKPYVVPGRGLRVVMVDFGAKHGILRELTRRDCHITVVPHNYSAEAILRLKPDGIMLTNGPGDPKDVPEAIEMIKQLLGQ
IPIFGICLGHQLLALACGADTEKMKFGHRGANHPVKDLLAGKTYLTSQNHSYAVNVSSLVNTDLELTQIALNDDTVEGIR
HTAFPAFSVQYHPEASPGPEDTNFLFDEFLNLIKASKVKQGGEVYA

Sequences:

>Translated_366_residues
MKKRKLILEDGTVFNGTAFGSDAESSGEIVFNTGMTGYQEVITDPSYCGQFVTLTYPLIGNYGINRDDFETVTPFIHGLV
VKEYSEFPSNFRNEETLDEFLQAHNIPGIANIDTRKLTRIIRKHGTMRAVMVDEQKNEQHVIEQLRLAEMPRDQVKRTST
IKPYVVPGRGLRVVMVDFGAKHGILRELTRRDCHITVVPHNYSAEAILRLKPDGIMLTNGPGDPKDVPEAIEMIKQLLGQ
IPIFGICLGHQLLALACGADTEKMKFGHRGANHPVKDLLAGKTYLTSQNHSYAVNVSSLVNTDLELTQIALNDDTVEGIR
HTAFPAFSVQYHPEASPGPEDTNFLFDEFLNLIKASKVKQGGEVYA
>Mature_366_residues
MKKRKLILEDGTVFNGTAFGSDAESSGEIVFNTGMTGYQEVITDPSYCGQFVTLTYPLIGNYGINRDDFETVTPFIHGLV
VKEYSEFPSNFRNEETLDEFLQAHNIPGIANIDTRKLTRIIRKHGTMRAVMVDEQKNEQHVIEQLRLAEMPRDQVKRTST
IKPYVVPGRGLRVVMVDFGAKHGILRELTRRDCHITVVPHNYSAEAILRLKPDGIMLTNGPGDPKDVPEAIEMIKQLLGQ
IPIFGICLGHQLLALACGADTEKMKFGHRGANHPVKDLLAGKTYLTSQNHSYAVNVSSLVNTDLELTQIALNDDTVEGIR
HTAFPAFSVQYHPEASPGPEDTNFLFDEFLNLIKASKVKQGGEVYA

Specific function: Arginine biosynthesis. Pyrimidine biosynthesis; first step. [C]

COG id: COG0505

COG function: function code EF; Carbamoylphosphate synthase small subunit

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 glutamine amidotransferase type-1 domain

Homologues:

Organism=Homo sapiens, GI18105007, Length=372, Percent_Identity=41.1290322580645, Blast_Score=270, Evalue=1e-72,
Organism=Homo sapiens, GI169790915, Length=374, Percent_Identity=35.8288770053476, Blast_Score=227, Evalue=1e-59,
Organism=Homo sapiens, GI21361331, Length=374, Percent_Identity=35.8288770053476, Blast_Score=227, Evalue=1e-59,
Organism=Escherichia coli, GI1786215, Length=377, Percent_Identity=46.9496021220159, Blast_Score=319, Evalue=2e-88,
Organism=Caenorhabditis elegans, GI193204318, Length=370, Percent_Identity=40.2702702702703, Blast_Score=251, Evalue=4e-67,
Organism=Saccharomyces cerevisiae, GI6322331, Length=395, Percent_Identity=38.7341772151899, Blast_Score=269, Evalue=6e-73,
Organism=Saccharomyces cerevisiae, GI6324878, Length=378, Percent_Identity=37.8306878306878, Blast_Score=253, Evalue=4e-68,
Organism=Drosophila melanogaster, GI45555749, Length=372, Percent_Identity=40.8602150537634, Blast_Score=256, Evalue=1e-68,
Organism=Drosophila melanogaster, GI24642586, Length=375, Percent_Identity=41.3333333333333, Blast_Score=256, Evalue=2e-68,

Paralogues:

None

Copy number: 620 Molecules/Cell In: Growth Phase, Minimal Media (Based on E. coli). 2599 Molecules/Cell In: Growth Phase, Glucose-minimal MOPS Media. 3,500 Molecules/Cell In: Glucose minimal media [C]

Swissprot (AC and ID): CARA_OCEIH (Q8CXH8)

Other databases:

- EMBL:   BA000028
- RefSeq:   NP_692411.1
- ProteinModelPortal:   Q8CXH8
- SMR:   Q8CXH8
- GeneID:   1017748
- GenomeReviews:   BA000028_GR
- KEGG:   oih:OB1490
- NMPDR:   fig|221109.1.peg.1492
- HOGENOM:   HBG286341
- OMA:   EADIPFF
- ProtClustDB:   PRK12564
- BioCyc:   OIHE221109:OB1490-MONOMER
- BRENDA:   6.3.5.5
- HAMAP:   MF_01209_B
- InterPro:   IPR006220
- InterPro:   IPR001317
- InterPro:   IPR006274
- InterPro:   IPR002474
- InterPro:   IPR011702
- InterPro:   IPR017926
- InterPro:   IPR000991
- PANTHER:   PTHR11405:SF4
- PRINTS:   PR00097
- PRINTS:   PR00099
- PRINTS:   PR00096
- TIGRFAMs:   TIGR01368

Pfam domain/function: PF00988 CPSase_sm_chain; PF00117 GATase; SSF52021 CP_synthsmall

EC number: =6.3.5.5

Molecular weight: Translated: 40741; Mature: 40741

Theoretical pI: Translated: 6.32; Mature: 6.32

Prosite motif: PS51273 GATASE_TYPE_1; PS00442 GATASE_TYPE_I

Important sites: ACT_SITE 247-247 ACT_SITE 332-332 ACT_SITE 334-334

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.1 %Cys     (Translated Protein)
2.5 %Met     (Translated Protein)
3.6 %Cys+Met (Translated Protein)
1.1 %Cys     (Mature Protein)
2.5 %Met     (Mature Protein)
3.6 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MKKRKLILEDGTVFNGTAFGSDAESSGEIVFNTGMTGYQEVITDPSYCGQFVTLTYPLIG
CCCCEEEEECCCEECCCCCCCCCCCCCCEEEECCCCHHHHHHCCHHHCCCEEEEEEEHHC
NYGINRDDFETVTPFIHGLVVKEYSEFPSNFRNEETLDEFLQAHNIPGIANIDTRKLTRI
CCCCCCCHHHHHHHHHHHHHHHHHHHHHHCCCCHHHHHHHHHHCCCCCCCCCCHHHHHHH
IRKHGTMRAVMVDEQKNEQHVIEQLRLAEMPRDQVKRTSTIKPYVVPGRGLRVVMVDFGA
HHHCCCEEEEEECCCCCHHHHHHHHHHHHCCHHHHHHHCCCCCEEECCCCCEEEEEECCC
KHGILRELTRRDCHITVVPHNYSAEAILRLKPDGIMLTNGPGDPKDVPEAIEMIKQLLGQ
CCHHHHHHHHCCCEEEEECCCCCCCEEEEECCCCEEEECCCCCHHHHHHHHHHHHHHHHC
IPIFGICLGHQLLALACGADTEKMKFGHRGANHPVKDLLAGKTYLTSQNHSYAVNVSSLV
CCEEEHHHHHHHHHHHCCCCHHHHHCCCCCCCCCHHHHHCCCEEEECCCCEEEEEHHHHH
NTDLELTQIALNDDTVEGIRHTAFPAFSVQYHPEASPGPEDTNFLFDEFLNLIKASKVKQ
CCCCEEEEEEECCCHHHHHHHHCCCEEEEEECCCCCCCCCCHHHHHHHHHHHHHHHHHCC
GGEVYA
CCCCCC
>Mature Secondary Structure
MKKRKLILEDGTVFNGTAFGSDAESSGEIVFNTGMTGYQEVITDPSYCGQFVTLTYPLIG
CCCCEEEEECCCEECCCCCCCCCCCCCCEEEECCCCHHHHHHCCHHHCCCEEEEEEEHHC
NYGINRDDFETVTPFIHGLVVKEYSEFPSNFRNEETLDEFLQAHNIPGIANIDTRKLTRI
CCCCCCCHHHHHHHHHHHHHHHHHHHHHHCCCCHHHHHHHHHHCCCCCCCCCCHHHHHHH
IRKHGTMRAVMVDEQKNEQHVIEQLRLAEMPRDQVKRTSTIKPYVVPGRGLRVVMVDFGA
HHHCCCEEEEEECCCCCHHHHHHHHHHHHCCHHHHHHHCCCCCEEECCCCCEEEEEECCC
KHGILRELTRRDCHITVVPHNYSAEAILRLKPDGIMLTNGPGDPKDVPEAIEMIKQLLGQ
CCHHHHHHHHCCCEEEEECCCCCCCEEEEECCCCEEEECCCCCHHHHHHHHHHHHHHHHC
IPIFGICLGHQLLALACGADTEKMKFGHRGANHPVKDLLAGKTYLTSQNHSYAVNVSSLV
CCEEEHHHHHHHHHHHCCCCHHHHHCCCCCCCCCHHHHHCCCEEEECCCCEEEEEHHHHH
NTDLELTQIALNDDTVEGIRHTAFPAFSVQYHPEASPGPEDTNFLFDEFLNLIKASKVKQ
CCCCEEEEEEECCCHHHHHHHHCCCEEEEEECCCCCCCCCCHHHHHHHHHHHHHHHHHCC
GGEVYA
CCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 12235376