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The map label for this gene is malZ [C]

Identifier: 23098640

GI number: 23098640

Start: 1239564

End: 1241060

Strand: Direct

Name: malZ [C]

Synonym: OB1185

Alternate gene names: 23098640

Gene position: 1239564-1241060 (Clockwise)

Preceding gene: 23098639

Following gene: 23098644

Centisome position: 34.14

GC content: 36.01

Gene sequence:

>1497_bases
ATGTTAAATAGAAATAAATTGCTTCTATTGCTTACAGTGATTTTTGTTTTATTGCCTGTAACTAATGTAAACGCAGAAGA
ACCAATCAATATACATAATGAAATTATTTATGATATTTTAGTTGATCGTTTTAATAATGGAGATTCTGAGGAAACGGAAG
AAATTGCTGTGGATGACCCGAATGCATACCACGGTGGCGATATTAAAGGTATAATTGATCAACTAGATCGACTAAAGGAA
ATGGGGATTACTACCATTTCATTATCCCCTTTAATGACTAATCAACAGAATGGTTATCACGGATATTGGGTCCTTGATTA
TTTTGAAATAGACGAGCAATTAGGAACCATGGAAGATCTTAATCAGTTGATTGAACAAGCACATCAACGTGATATGAAAG
TAGTTATGGATATGGTAATCAATTATGCTGCAGCATCACATGAAGTAACAGAAGACGAAACTAAAGCTGATTGGTGGACG
GAACCTTCTTACACAGCTGCATCTTGGTCTGCTAATGCACTTCAATGGAATTTGAATCAACCTGAAGTTGCGGAGAATAT
GATAAATGTTGCAGACTACTGGATGGAAGAGACGGAGATAGATGGTTTTACGTTACATGCTGTTGATCAAGCGCCAATTG
ACTTTCTTCAAGAATATACACAACATATAAAAGAAACAGATGAAGAATTTTATTTAATAGGAGATATATTAGATCCAGAA
TCTGCTAACATTCAAGAATTAGAAGAAACACCTCTGGATATTGTAGCTAATCCTATGCTGCATGAACGGTTAAAACAAGT
ATTCTCTGATGTGGATAATCCAGTTTCTGAGGTGTACCATCAATGGGAGAATTTAGAGACAGACTCTCCGTTAATTACAA
TTGATAATTTTACAGAAGAACGTTTTACCAATGCATATTCGAAGAACCAACGAAATTCACTGACAGCATGGACACTAGCA
TTGACATATATGTATACTACACCAGGAACACCTCTAATTGTTCAAGGTACGGAGTTGCCAATGTATGGTGGAGATGAAGA
GGAGTCTCAGCGACTTGTACCATTTAATAGTGGGGACCCAGAATTGAAAGAATTTCATACTAGAATAGCGTCTTTACGAA
ATGAATTTCCTGCACTACGACAAGGTGACATTGAACTAGTCGATACCGTTGATTCGATGATAGTCTATAAAAGAAGTTAT
GAAGGCCAAACGATGTATGTTGCGATTAACAATGGAAGTGAGTCTGCTTATGTAGATGTAACTGATGTTGATGCTAATAA
AGAGTTACGCGGCCACTTACAAGATAATTTGGTTCGTGAAAACCAAGAAGGGAATCATCGAATTGGAATACCACGTGAAT
CTGTAGAAGTGTATGAAGTACAGGATGATCATGGTCTTAACTGGTGGTTTATTGGATTTATTGTTATGGTTATTTTGGCC
TTTATTGCTGGAGTTATCTATTTGACAATAAAACAACGCAAGAGAGAAAAGAAATAA

Upstream 100 bases:

>100_bases
GTCTTTTCAACAAATATCAACGTGTAGTTGACTATTTTTTTTATTAAGGAATAATAGAAGGGGAGTCGAAGGGATTATTT
ACTAATGGAGGATGTACATC

Downstream 100 bases:

>100_bases
GTGTAAAAAAGGTTAAGACAGAAAGTAGTGAAAAGGTTAATGTGATTATTTATCCTTTTCAGGAATACTTTCATGAGGTC
TTAACCTTTTAATTGAACTA

Product: alpha-amylase

Products: NA

Alternate protein names: Beta-amylase; Alpha-amylase [H]

Number of amino acids: Translated: 498; Mature: 498

Protein sequence:

>498_residues
MLNRNKLLLLLTVIFVLLPVTNVNAEEPINIHNEIIYDILVDRFNNGDSEETEEIAVDDPNAYHGGDIKGIIDQLDRLKE
MGITTISLSPLMTNQQNGYHGYWVLDYFEIDEQLGTMEDLNQLIEQAHQRDMKVVMDMVINYAAASHEVTEDETKADWWT
EPSYTAASWSANALQWNLNQPEVAENMINVADYWMEETEIDGFTLHAVDQAPIDFLQEYTQHIKETDEEFYLIGDILDPE
SANIQELEETPLDIVANPMLHERLKQVFSDVDNPVSEVYHQWENLETDSPLITIDNFTEERFTNAYSKNQRNSLTAWTLA
LTYMYTTPGTPLIVQGTELPMYGGDEEESQRLVPFNSGDPELKEFHTRIASLRNEFPALRQGDIELVDTVDSMIVYKRSY
EGQTMYVAINNGSESAYVDVTDVDANKELRGHLQDNLVRENQEGNHRIGIPRESVEVYEVQDDHGLNWWFIGFIVMVILA
FIAGVIYLTIKQRKREKK

Sequences:

>Translated_498_residues
MLNRNKLLLLLTVIFVLLPVTNVNAEEPINIHNEIIYDILVDRFNNGDSEETEEIAVDDPNAYHGGDIKGIIDQLDRLKE
MGITTISLSPLMTNQQNGYHGYWVLDYFEIDEQLGTMEDLNQLIEQAHQRDMKVVMDMVINYAAASHEVTEDETKADWWT
EPSYTAASWSANALQWNLNQPEVAENMINVADYWMEETEIDGFTLHAVDQAPIDFLQEYTQHIKETDEEFYLIGDILDPE
SANIQELEETPLDIVANPMLHERLKQVFSDVDNPVSEVYHQWENLETDSPLITIDNFTEERFTNAYSKNQRNSLTAWTLA
LTYMYTTPGTPLIVQGTELPMYGGDEEESQRLVPFNSGDPELKEFHTRIASLRNEFPALRQGDIELVDTVDSMIVYKRSY
EGQTMYVAINNGSESAYVDVTDVDANKELRGHLQDNLVRENQEGNHRIGIPRESVEVYEVQDDHGLNWWFIGFIVMVILA
FIAGVIYLTIKQRKREKK
>Mature_498_residues
MLNRNKLLLLLTVIFVLLPVTNVNAEEPINIHNEIIYDILVDRFNNGDSEETEEIAVDDPNAYHGGDIKGIIDQLDRLKE
MGITTISLSPLMTNQQNGYHGYWVLDYFEIDEQLGTMEDLNQLIEQAHQRDMKVVMDMVINYAAASHEVTEDETKADWWT
EPSYTAASWSANALQWNLNQPEVAENMINVADYWMEETEIDGFTLHAVDQAPIDFLQEYTQHIKETDEEFYLIGDILDPE
SANIQELEETPLDIVANPMLHERLKQVFSDVDNPVSEVYHQWENLETDSPLITIDNFTEERFTNAYSKNQRNSLTAWTLA
LTYMYTTPGTPLIVQGTELPMYGGDEEESQRLVPFNSGDPELKEFHTRIASLRNEFPALRQGDIELVDTVDSMIVYKRSY
EGQTMYVAINNGSESAYVDVTDVDANKELRGHLQDNLVRENQEGNHRIGIPRESVEVYEVQDDHGLNWWFIGFIVMVILA
FIAGVIYLTIKQRKREKK

Specific function: The precursor protein is proteolytically cleaved to produce multiform beta-amylases and a 48 kDa alpha-amylase after secretion [H]

COG id: COG0366

COG function: function code G; Glycosidases

Gene ontology:

Cell location: Secreted [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: In the C-terminal section; belongs to the glycosyl hydrolase 13 family [H]

Homologues:

Organism=Escherichia coli, GI1786604, Length=481, Percent_Identity=23.4927234927235, Blast_Score=95, Evalue=1e-20,
Organism=Escherichia coli, GI1789995, Length=148, Percent_Identity=27.027027027027, Blast_Score=85, Evalue=1e-17,
Organism=Escherichia coli, GI1790687, Length=211, Percent_Identity=25.5924170616114, Blast_Score=70, Evalue=4e-13,
Organism=Saccharomyces cerevisiae, GI6322245, Length=115, Percent_Identity=34.7826086956522, Blast_Score=71, Evalue=4e-13,
Organism=Saccharomyces cerevisiae, GI6322241, Length=115, Percent_Identity=33.9130434782609, Blast_Score=66, Evalue=1e-11,
Organism=Saccharomyces cerevisiae, GI6322021, Length=115, Percent_Identity=33.9130434782609, Blast_Score=66, Evalue=1e-11,
Organism=Saccharomyces cerevisiae, GI6324416, Length=115, Percent_Identity=33.9130434782609, Blast_Score=66, Evalue=1e-11,
Organism=Saccharomyces cerevisiae, GI6321726, Length=115, Percent_Identity=33.9130434782609, Blast_Score=66, Evalue=2e-11,
Organism=Drosophila melanogaster, GI221330053, Length=181, Percent_Identity=26.5193370165746, Blast_Score=72, Evalue=1e-12,
Organism=Drosophila melanogaster, GI24586589, Length=540, Percent_Identity=22.7777777777778, Blast_Score=70, Evalue=2e-12,
Organism=Drosophila melanogaster, GI24586593, Length=189, Percent_Identity=28.5714285714286, Blast_Score=70, Evalue=3e-12,
Organism=Drosophila melanogaster, GI24586587, Length=181, Percent_Identity=27.0718232044199, Blast_Score=70, Evalue=4e-12,
Organism=Drosophila melanogaster, GI45549022, Length=181, Percent_Identity=25.9668508287293, Blast_Score=70, Evalue=4e-12,
Organism=Drosophila melanogaster, GI24586597, Length=211, Percent_Identity=27.0142180094787, Blast_Score=69, Evalue=1e-11,
Organism=Drosophila melanogaster, GI24586599, Length=181, Percent_Identity=27.6243093922652, Blast_Score=67, Evalue=2e-11,
Organism=Drosophila melanogaster, GI24586591, Length=187, Percent_Identity=26.2032085561497, Blast_Score=67, Evalue=3e-11,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR006048
- InterPro:   IPR005085
- InterPro:   IPR008985
- InterPro:   IPR013780
- InterPro:   IPR006047
- InterPro:   IPR006589
- InterPro:   IPR001554
- InterPro:   IPR018238
- InterPro:   IPR000125
- InterPro:   IPR017853
- InterPro:   IPR013781 [H]

Pfam domain/function: PF00128 Alpha-amylase; PF03423 CBM_25; PF01373 Glyco_hydro_14 [H]

EC number: =3.2.1.2; =3.2.1.1 [H]

Molecular weight: Translated: 57287; Mature: 57287

Theoretical pI: Translated: 4.04; Mature: 4.04

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.0 %Cys     (Translated Protein)
3.0 %Met     (Translated Protein)
3.0 %Cys+Met (Translated Protein)
0.0 %Cys     (Mature Protein)
3.0 %Met     (Mature Protein)
3.0 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MLNRNKLLLLLTVIFVLLPVTNVNAEEPINIHNEIIYDILVDRFNNGDSEETEEIAVDDP
CCCCCHHHHHHHHHHHHHCCCCCCCCCCCCHHHHHHHHHHHHHCCCCCCCCCCEEECCCC
NAYHGGDIKGIIDQLDRLKEMGITTISLSPLMTNQQNGYHGYWVLDYFEIDEQLGTMEDL
CCCCCCCHHHHHHHHHHHHHCCCEEEEECCCCCCCCCCEEEEEEEEEEEHHHHHCCHHHH
NQLIEQAHQRDMKVVMDMVINYAAASHEVTEDETKADWWTEPSYTAASWSANALQWNLNQ
HHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCCCCCCCCCCCCCCCCCCEEECCCC
PEVAENMINVADYWMEETEIDGFTLHAVDQAPIDFLQEYTQHIKETDEEFYLIGDILDPE
CHHHHHHHHHHHHHHHHCCCCCEEEEECCCCHHHHHHHHHHHHHCCCCCEEEEEEECCCC
SANIQELEETPLDIVANPMLHERLKQVFSDVDNPVSEVYHQWENLETDSPLITIDNFTEE
CCCHHHHHCCCHHHHCCHHHHHHHHHHHHHCCHHHHHHHHHHHCCCCCCCEEEECCCHHH
RFTNAYSKNQRNSLTAWTLALTYMYTTPGTPLIVQGTELPMYGGDEEESQRLVPFNSGDP
HHHHHHHCCCCCCHHEEEEEEEEEEECCCCEEEEECCCCCCCCCCCHHCCEECCCCCCCH
ELKEFHTRIASLRNEFPALRQGDIELVDTVDSMIVYKRSYEGQTMYVAINNGSESAYVDV
HHHHHHHHHHHHHHHCCCHHCCCCHHHHHHHHHHEEEECCCCCEEEEEEECCCCCEEEEE
TDVDANKELRGHLQDNLVRENQEGNHRIGIPRESVEVYEVQDDHGLNWWFIGFIVMVILA
EECCCCHHHHHHHHHHHHHCCCCCCCEECCCCCCCEEEEECCCCCCCHHHHHHHHHHHHH
FIAGVIYLTIKQRKREKK
HHHHHHHHHHHHHHCCCC
>Mature Secondary Structure
MLNRNKLLLLLTVIFVLLPVTNVNAEEPINIHNEIIYDILVDRFNNGDSEETEEIAVDDP
CCCCCHHHHHHHHHHHHHCCCCCCCCCCCCHHHHHHHHHHHHHCCCCCCCCCCEEECCCC
NAYHGGDIKGIIDQLDRLKEMGITTISLSPLMTNQQNGYHGYWVLDYFEIDEQLGTMEDL
CCCCCCCHHHHHHHHHHHHHCCCEEEEECCCCCCCCCCEEEEEEEEEEEHHHHHCCHHHH
NQLIEQAHQRDMKVVMDMVINYAAASHEVTEDETKADWWTEPSYTAASWSANALQWNLNQ
HHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCCCCCCCCCCCCCCCCCCEEECCCC
PEVAENMINVADYWMEETEIDGFTLHAVDQAPIDFLQEYTQHIKETDEEFYLIGDILDPE
CHHHHHHHHHHHHHHHHCCCCCEEEEECCCCHHHHHHHHHHHHHCCCCCEEEEEEECCCC
SANIQELEETPLDIVANPMLHERLKQVFSDVDNPVSEVYHQWENLETDSPLITIDNFTEE
CCCHHHHHCCCHHHHCCHHHHHHHHHHHHHCCHHHHHHHHHHHCCCCCCCEEEECCCHHH
RFTNAYSKNQRNSLTAWTLALTYMYTTPGTPLIVQGTELPMYGGDEEESQRLVPFNSGDP
HHHHHHHCCCCCCHHEEEEEEEEEEECCCCEEEEECCCCCCCCCCCHHCCEECCCCCCCH
ELKEFHTRIASLRNEFPALRQGDIELVDTVDSMIVYKRSYEGQTMYVAINNGSESAYVDV
HHHHHHHHHHHHHHHCCCHHCCCCHHHHHHHHHHEEEECCCCCEEEEEEECCCCCEEEEE
TDVDANKELRGHLQDNLVRENQEGNHRIGIPRESVEVYEVQDDHGLNWWFIGFIVMVILA
EECCCCHHHHHHHHHHHHHCCCCCCCEECCCCCCCEEEEECCCCCCCHHHHHHHHHHHHH
FIAGVIYLTIKQRKREKK
HHHHHHHHHHHHHHCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 2435707; 2464578; 2438660; 1827035 [H]