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The map label for this gene is lplA [H]

Identifier: 23098629

GI number: 23098629

Start: 1224004

End: 1224999

Strand: Direct

Name: lplA [H]

Synonym: OB1174

Alternate gene names: 23098629

Gene position: 1224004-1224999 (Clockwise)

Preceding gene: 23098628

Following gene: 23098630

Centisome position: 33.71

GC content: 33.13

Gene sequence:

>996_bases
ATGAAATTTATTGATAATAAAGGCATTACCGATCCACGTATTAACTTAGCCATTGAAGAATACATCTTAGAAAACTTTGG
TGAAAAAGATACATATCTTCTATTCTATGTGAATAGCCCGTCCATTATTATTGGCCGTAATCAGAATACCATTGAGGAAA
TAAATACAGATTATGTAGATGAAAAAGGTATAAAGGTTGTCCGTCGTTTGTCAGGTGGGGGAGCAGTATACCATGATGAA
CAAAATCTTAACTTTAGCTTCATTACAAAGGATGATGGGAATAGCTTTCAAAACTTCGCGAAATTTACACAGCCTGTGGT
GGATGCATTAAATAAAGTTGGTGTACCAGCGGAATTACAGGGAAGAAATGATTTAGCAGCAGATGGACGTAAAATATCTG
GTAATGCAATGTTCTCTACTAGAGGGCGTATGTTTAGTCATGGGACTTTAATGTTAGATTCTGAACTAGAAGAATTAACA
AAAGCATTAAAGGTGAAAAAAATTAAAATTGAATCAAAAGGAATTAAATCCATTCGTAGTCGAGTAGCTAATATCGCAGA
ATTCCTCGATGAAAAAATAACAATGGAGCAATTTAAAGAATTAATTTTAACACATGTGTTTGACGTAGAAGATGTGAACG
ACGTACCACAGTATGTATTAACAGATGAAGACTGGAAAAAAATCCATGAAATATCAGAAAAACGTTATCAAAAATGGGAA
TGGAACTATGGAAAATCTCCTTCATTTAATTTCCAAGAATCACAAAAATTTCAAGGTGGACTTGTAGATGTACGCTTAGA
TGTAAAAAAAGGTATGATCGAAAACTGTAAGATCTACGGTGACTTCTTTGGAATAGGCTTAATTAATGAATTAGAAAATC
ACTTGATTGGTATACGTCATAATCGTCAATCGATTGAAGAAGCTTTAAAGGATGTTGATGTGTCCCACTATTTAGGTAGA
ATTACAAAAGAAGAATTCATTTCTCTGCTTTATTAA

Upstream 100 bases:

>100_bases
CGTGGTATGGGAATTAAGGTCTATTTCTTTTGATTACGTGAAAGGATTGTTCTACAATAAAAGAAAGCATATAAATATGT
GAATTATGGGAGGTTTTACA

Downstream 100 bases:

>100_bases
GGTAGGATAACTTTTTAATAGATATAATAAAAATCCCGAGTTATATAACTACTACAAGGTTATAACTCGGTTTTTTTATC
TGGTACTTGAAAGCTGTGAA

Product: lipoate protein ligase

Products: NA

Alternate protein names: Lipoate--protein ligase [H]

Number of amino acids: Translated: 331; Mature: 331

Protein sequence:

>331_residues
MKFIDNKGITDPRINLAIEEYILENFGEKDTYLLFYVNSPSIIIGRNQNTIEEINTDYVDEKGIKVVRRLSGGGAVYHDE
QNLNFSFITKDDGNSFQNFAKFTQPVVDALNKVGVPAELQGRNDLAADGRKISGNAMFSTRGRMFSHGTLMLDSELEELT
KALKVKKIKIESKGIKSIRSRVANIAEFLDEKITMEQFKELILTHVFDVEDVNDVPQYVLTDEDWKKIHEISEKRYQKWE
WNYGKSPSFNFQESQKFQGGLVDVRLDVKKGMIENCKIYGDFFGIGLINELENHLIGIRHNRQSIEEALKDVDVSHYLGR
ITKEEFISLLY

Sequences:

>Translated_331_residues
MKFIDNKGITDPRINLAIEEYILENFGEKDTYLLFYVNSPSIIIGRNQNTIEEINTDYVDEKGIKVVRRLSGGGAVYHDE
QNLNFSFITKDDGNSFQNFAKFTQPVVDALNKVGVPAELQGRNDLAADGRKISGNAMFSTRGRMFSHGTLMLDSELEELT
KALKVKKIKIESKGIKSIRSRVANIAEFLDEKITMEQFKELILTHVFDVEDVNDVPQYVLTDEDWKKIHEISEKRYQKWE
WNYGKSPSFNFQESQKFQGGLVDVRLDVKKGMIENCKIYGDFFGIGLINELENHLIGIRHNRQSIEEALKDVDVSHYLGR
ITKEEFISLLY
>Mature_331_residues
MKFIDNKGITDPRINLAIEEYILENFGEKDTYLLFYVNSPSIIIGRNQNTIEEINTDYVDEKGIKVVRRLSGGGAVYHDE
QNLNFSFITKDDGNSFQNFAKFTQPVVDALNKVGVPAELQGRNDLAADGRKISGNAMFSTRGRMFSHGTLMLDSELEELT
KALKVKKIKIESKGIKSIRSRVANIAEFLDEKITMEQFKELILTHVFDVEDVNDVPQYVLTDEDWKKIHEISEKRYQKWE
WNYGKSPSFNFQESQKFQGGLVDVRLDVKKGMIENCKIYGDFFGIGLINELENHLIGIRHNRQSIEEALKDVDVSHYLGR
ITKEEFISLLY

Specific function: Catalyzes both the ATP-dependent activation of exogenously supplied lipoate to lipoyl-AMP and the transfer of the activated lipoyl onto the lipoyl domains of lipoate-dependent enzymes [H]

COG id: COG0095

COG function: function code H; Lipoate-protein ligase A

Gene ontology:

Cell location: Cytoplasm [H]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the lplA family [H]

Homologues:

Organism=Homo sapiens, GI7706252, Length=295, Percent_Identity=34.9152542372881, Blast_Score=133, Evalue=2e-31,
Organism=Homo sapiens, GI21729884, Length=295, Percent_Identity=34.9152542372881, Blast_Score=133, Evalue=2e-31,
Organism=Homo sapiens, GI21729882, Length=295, Percent_Identity=34.9152542372881, Blast_Score=133, Evalue=2e-31,
Organism=Homo sapiens, GI21729880, Length=295, Percent_Identity=34.9152542372881, Blast_Score=133, Evalue=2e-31,
Organism=Homo sapiens, GI21729878, Length=295, Percent_Identity=34.9152542372881, Blast_Score=133, Evalue=2e-31,
Organism=Escherichia coli, GI1790846, Length=294, Percent_Identity=36.0544217687075, Blast_Score=178, Evalue=3e-46,
Organism=Caenorhabditis elegans, GI71981909, Length=250, Percent_Identity=34.4, Blast_Score=125, Evalue=3e-29,
Organism=Saccharomyces cerevisiae, GI6322415, Length=306, Percent_Identity=30.3921568627451, Blast_Score=122, Evalue=1e-28,
Organism=Drosophila melanogaster, GI28573464, Length=282, Percent_Identity=28.0141843971631, Blast_Score=108, Evalue=5e-24,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR004143
- InterPro:   IPR005107
- InterPro:   IPR019491
- InterPro:   IPR004562 [H]

Pfam domain/function: PF03099 BPL_LipA_LipB; PF10437 Lip_prot_lig_C [H]

EC number: =2.7.7.63 [H]

Molecular weight: Translated: 38066; Mature: 38066

Theoretical pI: Translated: 5.34; Mature: 5.34

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.3 %Cys     (Translated Protein)
1.8 %Met     (Translated Protein)
2.1 %Cys+Met (Translated Protein)
0.3 %Cys     (Mature Protein)
1.8 %Met     (Mature Protein)
2.1 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MKFIDNKGITDPRINLAIEEYILENFGEKDTYLLFYVNSPSIIIGRNQNTIEEINTDYVD
CCCCCCCCCCCCHHHHHHHHHHHHHCCCCCEEEEEEECCCEEEEECCCCHHHHHCCCCCC
EKGIKVVRRLSGGGAVYHDEQNLNFSFITKDDGNSFQNFAKFTQPVVDALNKVGVPAELQ
HHHHHHHHHHCCCCEEEECCCCCCEEEEECCCCCHHHHHHHHHHHHHHHHHHCCCCCEEC
GRNDLAADGRKISGNAMFSTRGRMFSHGTLMLDSELEELTKALKVKKIKIESKGIKSIRS
CCCCCCCCCCEECCCEEEECCCCEEECCEEEEHHHHHHHHHHHHHHHEEECHHHHHHHHH
RVANIAEFLDEKITMEQFKELILTHVFDVEDVNDVPQYVLTDEDWKKIHEISEKRYQKWE
HHHHHHHHHHHHCCHHHHHHHHHHHHCCCCCCCCCCHHEECCHHHHHHHHHHHHHHHHCC
WNYGKSPSFNFQESQKFQGGLVDVRLDVKKGMIENCKIYGDFFGIGLINELENHLIGIRH
CCCCCCCCCCCCHHHHCCCCEEEEEEEHHHCCHHCCEEEEHHHHHHHHHHHHHHHEEECC
NRQSIEEALKDVDVSHYLGRITKEEFISLLY
CHHHHHHHHHHCCHHHHHHHCCHHHHHHHCC
>Mature Secondary Structure
MKFIDNKGITDPRINLAIEEYILENFGEKDTYLLFYVNSPSIIIGRNQNTIEEINTDYVD
CCCCCCCCCCCCHHHHHHHHHHHHHCCCCCEEEEEEECCCEEEEECCCCHHHHHCCCCCC
EKGIKVVRRLSGGGAVYHDEQNLNFSFITKDDGNSFQNFAKFTQPVVDALNKVGVPAELQ
HHHHHHHHHHCCCCEEEECCCCCCEEEEECCCCCHHHHHHHHHHHHHHHHHHCCCCCEEC
GRNDLAADGRKISGNAMFSTRGRMFSHGTLMLDSELEELTKALKVKKIKIESKGIKSIRS
CCCCCCCCCCEECCCEEEECCCCEEECCEEEEHHHHHHHHHHHHHHHEEECHHHHHHHHH
RVANIAEFLDEKITMEQFKELILTHVFDVEDVNDVPQYVLTDEDWKKIHEISEKRYQKWE
HHHHHHHHHHHHCCHHHHHHHHHHHHCCCCCCCCCCHHEECCHHHHHHHHHHHHHHHHCC
WNYGKSPSFNFQESQKFQGGLVDVRLDVKKGMIENCKIYGDFFGIGLINELENHLIGIRH
CCCCCCCCCCCCHHHHCCCCEEEEEEEHHHCCHHCCEEEEHHHHHHHHHHHHHHHEEECC
NRQSIEEALKDVDVSHYLGRITKEEFISLLY
CHHHHHHHHHHCCHHHHHHHCCHHHHHHHCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 9579061; 9384377 [H]