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The map label for this gene is yibK [C]

Identifier: 23098542

GI number: 23098542

Start: 1135412

End: 1135885

Strand: Direct

Name: yibK [C]

Synonym: OB1087

Alternate gene names: 23098542

Gene position: 1135412-1135885 (Clockwise)

Preceding gene: 23098541

Following gene: 23098544

Centisome position: 31.27

GC content: 34.6

Gene sequence:

>474_bases
TTGAGTGTGCATGTTGTTTTATTTGAACCAGAAATCCCAGCTAATACGGGAAATATAGGCAGAACATGTCTTGCAACAGG
GGTTACACTCCATCTAATCCATCCACTTGGCTTCTCTACAGATAGTAAAATGGTACGAAGAGCTGGATTGGATTATTGGG
AGCATGTAGATGTTCGAGAATATCAATCAATAGAAGACCTTTATGAAACATACCCAGATGCTGGATTTTATTATATTGAG
AACTTTGGAACCAAACATTATACTGACTTTGATTTTAGTGATTCGGACATGGATCAATTCTTTGTTTTTGGAAAAGAGTC
CACAGGTATACCCAAAGAATTGCTGATAGGAAAAGAAGACCAATGTCTGCGAATTCATATGAATGATAAAGTAAGATCAC
TAAACTTATCTAATACTTCAGCAATAATTATTTATGAAGTATTAAGGCAACAAGGCTTTCCTAATTTATATTAA

Upstream 100 bases:

>100_bases
TCTACGGCATACACACCAGAAATCCAGTACAAATTTTTTCGAATTGGTGAAAATTAGTGTATAATGTATAAGGTTACTAA
AGAAGAAGGAGAAATGAGTT

Downstream 100 bases:

>100_bases
TATGTATCCTACTTCGTAATGGAGTAGGTTTTTTGTTACCTACCAAAAAAGGGGGGGCAAATAGATGAAAGAAGTTAGAA
GGAGCCCCTTTGAAATTCAA

Product: rRNA methylase

Products: NA

Alternate protein names: ORF3 [H]

Number of amino acids: Translated: 157; Mature: 156

Protein sequence:

>157_residues
MSVHVVLFEPEIPANTGNIGRTCLATGVTLHLIHPLGFSTDSKMVRRAGLDYWEHVDVREYQSIEDLYETYPDAGFYYIE
NFGTKHYTDFDFSDSDMDQFFVFGKESTGIPKELLIGKEDQCLRIHMNDKVRSLNLSNTSAIIIYEVLRQQGFPNLY

Sequences:

>Translated_157_residues
MSVHVVLFEPEIPANTGNIGRTCLATGVTLHLIHPLGFSTDSKMVRRAGLDYWEHVDVREYQSIEDLYETYPDAGFYYIE
NFGTKHYTDFDFSDSDMDQFFVFGKESTGIPKELLIGKEDQCLRIHMNDKVRSLNLSNTSAIIIYEVLRQQGFPNLY
>Mature_156_residues
SVHVVLFEPEIPANTGNIGRTCLATGVTLHLIHPLGFSTDSKMVRRAGLDYWEHVDVREYQSIEDLYETYPDAGFYYIEN
FGTKHYTDFDFSDSDMDQFFVFGKESTGIPKELLIGKEDQCLRIHMNDKVRSLNLSNTSAIIIYEVLRQQGFPNLY

Specific function: Could methylate the ribose at the nucleotide 34 wobble position in tRNA [H]

COG id: COG0219

COG function: function code J; Predicted rRNA methylase (SpoU class)

Gene ontology:

Cell location: Cytoplasm (Potential) [H]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the RNA methyltransferase TrmH family. TrmL subfamily [H]

Homologues:

Organism=Escherichia coli, GI1790034, Length=153, Percent_Identity=39.2156862745098, Blast_Score=119, Evalue=9e-29,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR004440
- InterPro:   IPR016914
- InterPro:   IPR001537 [H]

Pfam domain/function: PF00588 SpoU_methylase [H]

EC number: 2.1.1.- [C]

Molecular weight: Translated: 18070; Mature: 17938

Theoretical pI: Translated: 4.68; Mature: 4.68

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.3 %Cys     (Translated Protein)
2.5 %Met     (Translated Protein)
3.8 %Cys+Met (Translated Protein)
1.3 %Cys     (Mature Protein)
1.9 %Met     (Mature Protein)
3.2 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MSVHVVLFEPEIPANTGNIGRTCLATGVTLHLIHPLGFSTDSKMVRRAGLDYWEHVDVRE
CEEEEEEECCCCCCCCCCCCCHHHHHCCEEEEEECCCCCCHHHHHHHHCCCHHHCCCHHH
YQSIEDLYETYPDAGFYYIENFGTKHYTDFDFSDSDMDQFFVFGKESTGIPKELLIGKED
HHHHHHHHHHCCCCCEEEEECCCCCCCCCCCCCCCCCCEEEEECCCCCCCCHHHHCCCCC
QCLRIHMNDKVRSLNLSNTSAIIIYEVLRQQGFPNLY
CEEEEEECCCEEEEECCCCCCHHHHHHHHHCCCCCCC
>Mature Secondary Structure 
SVHVVLFEPEIPANTGNIGRTCLATGVTLHLIHPLGFSTDSKMVRRAGLDYWEHVDVRE
EEEEEEECCCCCCCCCCCCCHHHHHCCEEEEEECCCCCCHHHHHHHHCCCHHHCCCHHH
YQSIEDLYETYPDAGFYYIENFGTKHYTDFDFSDSDMDQFFVFGKESTGIPKELLIGKED
HHHHHHHHHHCCCCCEEEEECCCCCCCCCCCCCCCCCCEEEEECCCCCCCCHHHHCCCCC
QCLRIHMNDKVRSLNLSNTSAIIIYEVLRQQGFPNLY
CEEEEEECCCEEEEECCCCCCHHHHHHHHHCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 1339360 [H]