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The map label for this gene is daaA [H]

Identifier: 23098538

GI number: 23098538

Start: 1129963

End: 1130829

Strand: Reverse

Name: daaA [H]

Synonym: OB1083

Alternate gene names: 23098538

Gene position: 1130829-1129963 (Counterclockwise)

Preceding gene: 23098543

Following gene: 23098537

Centisome position: 31.15

GC content: 35.29

Gene sequence:

>867_bases
ATGTCTGTATATCCAATTATTTTAACGCAATCTGAATTTAAAGATAAGGATAGCCTAAACTACCCATTTGAAGAACGTGG
TTTGCAATTCGGAGATGGTATTTATGAAGTTATCCGTGTATATGAGGGGAAATACTATTTATTTGAAGAACATGTCGATC
GATTATATCGTTCTGCAGAAGCAATTAAAATTGAAATACCTCAAAGTAAAAAAGAAATGAAAGAATTATTGCTTCAACTA
ATTACAAAAAATGAAATGACTACAGATGGAAAAGTGTATATGCAAATCACAAGAGGGTCTGCTCCTCGCGATCATATCTT
CCCACAATCTGTAGAACCAAATATGTATGCCTATGTACAGGATATGCCAAGAAATAAAGAAGCTCTACAACATGGAGTAT
CAACGATTACTCAACCTGATATTCGTTGGGAAAATTGCTATATTAAAAGTTTAAACTTACTTCCTAATGTTCTAGCAAAA
CAGACAGCTAAAGAACAAGGATGTTATGAGGCTATTCTCCATAGAGACGGCAAAGTCACTGAATGCAGTGCATCCAATGT
GTATTTAGTTAAAAACGGGAAAGTATATACTCACCCAACAACAAACAATATACTCTACGGTTGTGTGCGCATGGCAGTGG
AAAGGTTCTTAAAAGCTGCAAATATTGAACTTATTGAAGAAGCGTTTCAACTAGAAGACATTTCTAGTGCAGATGAAATA
TTCTTATCCAGTAGTACTTCTGAAGTTACGCCCATTGTACGTGTGGATGATAAAGCTATTGGAGACGGTAAGCCAGGTAC
AATCACCAAGCAATTACAGCGAGCATATGAAACAGAAGCAGCTATAAAAGATAAATTAACTGTGTAA

Upstream 100 bases:

>100_bases
ATTGACGCATTTATATAGATAATTATGAAATAATTAGTTATTTACATATGATATAGCAAGTTATAAACTAGACTTATGCT
TGAAGAGAGGAGCTTATTTT

Downstream 100 bases:

>100_bases
CATTCTTTTTTATGGTCTAAAATTTTGGAGCTGTGGATAAACAACTAAGTTAATTAGCCACGTCCAGCTCCAGCGCCCAT
CACCTATTGTCCTTCCGGAG

Product: D-alanine aminotransferase

Products: NA

Alternate protein names: D-amino acid aminotransferase; D-amino acid transaminase; DAAT; D-aspartate aminotransferase [H]

Number of amino acids: Translated: 288; Mature: 287

Protein sequence:

>288_residues
MSVYPIILTQSEFKDKDSLNYPFEERGLQFGDGIYEVIRVYEGKYYLFEEHVDRLYRSAEAIKIEIPQSKKEMKELLLQL
ITKNEMTTDGKVYMQITRGSAPRDHIFPQSVEPNMYAYVQDMPRNKEALQHGVSTITQPDIRWENCYIKSLNLLPNVLAK
QTAKEQGCYEAILHRDGKVTECSASNVYLVKNGKVYTHPTTNNILYGCVRMAVERFLKAANIELIEEAFQLEDISSADEI
FLSSSTSEVTPIVRVDDKAIGDGKPGTITKQLQRAYETEAAIKDKLTV

Sequences:

>Translated_288_residues
MSVYPIILTQSEFKDKDSLNYPFEERGLQFGDGIYEVIRVYEGKYYLFEEHVDRLYRSAEAIKIEIPQSKKEMKELLLQL
ITKNEMTTDGKVYMQITRGSAPRDHIFPQSVEPNMYAYVQDMPRNKEALQHGVSTITQPDIRWENCYIKSLNLLPNVLAK
QTAKEQGCYEAILHRDGKVTECSASNVYLVKNGKVYTHPTTNNILYGCVRMAVERFLKAANIELIEEAFQLEDISSADEI
FLSSSTSEVTPIVRVDDKAIGDGKPGTITKQLQRAYETEAAIKDKLTV
>Mature_287_residues
SVYPIILTQSEFKDKDSLNYPFEERGLQFGDGIYEVIRVYEGKYYLFEEHVDRLYRSAEAIKIEIPQSKKEMKELLLQLI
TKNEMTTDGKVYMQITRGSAPRDHIFPQSVEPNMYAYVQDMPRNKEALQHGVSTITQPDIRWENCYIKSLNLLPNVLAKQ
TAKEQGCYEAILHRDGKVTECSASNVYLVKNGKVYTHPTTNNILYGCVRMAVERFLKAANIELIEEAFQLEDISSADEIF
LSSSTSEVTPIVRVDDKAIGDGKPGTITKQLQRAYETEAAIKDKLTV

Specific function: Acts on the D-isomers of alanine, leucine, aspartate, glutamate, aminobutyrate, norvaline and asparagine. The enzyme transfers an amino group from a substrate D-amino acid to the pyridoxal phosphate cofactor to form pyridoxamine and an alpha- keto acid in

COG id: COG0115

COG function: function code EH; Branched-chain amino acid aminotransferase/4-amino-4-deoxychorismate lyase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the class-IV pyridoxal-phosphate-dependent aminotransferase family [H]

Homologues:

Organism=Escherichia coli, GI48994963, Length=270, Percent_Identity=27.4074074074074, Blast_Score=106, Evalue=2e-24,
Organism=Escherichia coli, GI1787338, Length=241, Percent_Identity=28.6307053941909, Blast_Score=87, Evalue=1e-18,

Paralogues:

None

Copy number: 2342 Molecules/Cell In: Growth Phase, Glucose-minimal MOPS Media. 11,000 Molecules/Cell In: Glucose minimal media [C]

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR001544
- InterPro:   IPR018300
- InterPro:   IPR005784 [H]

Pfam domain/function: PF01063 Aminotran_4 [H]

EC number: =2.6.1.21 [H]

Molecular weight: Translated: 32868; Mature: 32737

Theoretical pI: Translated: 5.28; Mature: 5.28

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.4 %Cys     (Translated Protein)
2.4 %Met     (Translated Protein)
3.8 %Cys+Met (Translated Protein)
1.4 %Cys     (Mature Protein)
2.1 %Met     (Mature Protein)
3.5 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MSVYPIILTQSEFKDKDSLNYPFEERGLQFGDGIYEVIRVYEGKYYLFEEHVDRLYRSAE
CCEEEEEEECCCCCCCCCCCCCHHHCCCCCCHHHHHHHHHHCCCEEEHHHHHHHHHHCCC
AIKIEIPQSKKEMKELLLQLITKNEMTTDGKVYMQITRGSAPRDHIFPQSVEPNMYAYVQ
EEEEECCCCHHHHHHHHHHHHHCCCCCCCCEEEEEEECCCCCCCCCCCCCCCCCCEEEEE
DMPRNKEALQHGVSTITQPDIRWENCYIKSLNLLPNVLAKQTAKEQGCYEAILHRDGKVT
CCCCCHHHHHHHHHHCCCCCCEECCEEEEHHHHHHHHHHHHHHHHHHHHHHHHHCCCCEE
ECSASNVYLVKNGKVYTHPTTNNILYGCVRMAVERFLKAANIELIEEAFQLEDISSADEI
EECCCCEEEEECCEEEECCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCHHHE
FLSSSTSEVTPIVRVDDKAIGDGKPGTITKQLQRAYETEAAIKDKLTV
EEECCCCCCCEEEEECCCCCCCCCCCHHHHHHHHHHHHHHHHHHCCCC
>Mature Secondary Structure 
SVYPIILTQSEFKDKDSLNYPFEERGLQFGDGIYEVIRVYEGKYYLFEEHVDRLYRSAE
CEEEEEEECCCCCCCCCCCCCHHHCCCCCCHHHHHHHHHHCCCEEEHHHHHHHHHHCCC
AIKIEIPQSKKEMKELLLQLITKNEMTTDGKVYMQITRGSAPRDHIFPQSVEPNMYAYVQ
EEEEECCCCHHHHHHHHHHHHHCCCCCCCCEEEEEEECCCCCCCCCCCCCCCCCCEEEEE
DMPRNKEALQHGVSTITQPDIRWENCYIKSLNLLPNVLAKQTAKEQGCYEAILHRDGKVT
CCCCCHHHHHHHHHHCCCCCCEECCEEEEHHHHHHHHHHHHHHHHHHHHHHHHHCCCCEE
ECSASNVYLVKNGKVYTHPTTNNILYGCVRMAVERFLKAANIELIEEAFQLEDISSADEI
EECCCCEEEEECCEEEECCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCHHHE
FLSSSTSEVTPIVRVDDKAIGDGKPGTITKQLQRAYETEAAIKDKLTV
EEECCCCCCCEEEEECCCCCCCCCCCHHHHHHHHHHHHHHHHHHCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 2644261; 2914916; 7626635; 9538014; 9749913 [H]