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The map label for this gene is yfiK [H]

Identifier: 23098524

GI number: 23098524

Start: 1111625

End: 1112272

Strand: Direct

Name: yfiK [H]

Synonym: OB1069

Alternate gene names: 23098524

Gene position: 1111625-1112272 (Clockwise)

Preceding gene: 23098523

Following gene: 23098525

Centisome position: 30.62

GC content: 35.96

Gene sequence:

>648_bases
GTGGGGGTTATATTGTATCGAGTGTTGTTAGTAGAAGATCAGCAAATAGTTCGGCAAGGGATAAAAATATTATTAGAACA
AGATAAGCAAATTAAAGTTATTGGTGAAGCTACAAATGGTGTGGAAGCAATAGAACAAATGATGGAACATACGGTAGATG
TTATTTTAATGGATGTACGTATGCCAGATATGAATGGAATTGAAGCAACAAGGAAAATCAAATCCCAGTGGCCAAATGTA
CGTATTCTTATGTTGACGACGTTTAATGATGATGAATATACGTTGCAATCATTAAAAGAAGGGGCGAATGGGTTTTTATT
AAAAACTGCCGATGGAAAGAAATTAATTGACGCAATTCATAGCTGCATGAGAGGTGGATTAGTACTTCATGATGAAGTGG
CAGCAAAAGTAATGCCGAAATTATTGCATACATCGAAGAGGGAATTATCTGTTCAGCTTTCAGAACGGGAGCGTGTAATT
ACTGCGTTAATTGGGAGTGGATTAACGAATAAGGAAATCGCAGATGAATTATTTCTAACGATTGGAACAGTAAAAAATTA
TATCACTTCGATACTGCAAAAAACCGAACAAAGAGATCGCACACAACTCGCTATTTTTGCAGTCAGACATGGGATTACAA
AAGATTAA

Upstream 100 bases:

>100_bases
AGAGAGTGGTAGAGGTTAACGGGACCTTAAATGCTTATAAATTAGAAAAACGTTTTATTGTATCAGGAAGAATCCCTGTG
TAGGAGGCTTGTTAAGAAAG

Downstream 100 bases:

>100_bases
ATGTCATTGAATTGTTACAACTATATGGATCCAAAATCAAGTTAATGAATTATAATTAAGATATTCTAAGGATAAAAGTC
GTGGTGATTACATGATTGAA

Product: two-component response regulator

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 215; Mature: 214

Protein sequence:

>215_residues
MGVILYRVLLVEDQQIVRQGIKILLEQDKQIKVIGEATNGVEAIEQMMEHTVDVILMDVRMPDMNGIEATRKIKSQWPNV
RILMLTTFNDDEYTLQSLKEGANGFLLKTADGKKLIDAIHSCMRGGLVLHDEVAAKVMPKLLHTSKRELSVQLSERERVI
TALIGSGLTNKEIADELFLTIGTVKNYITSILQKTEQRDRTQLAIFAVRHGITKD

Sequences:

>Translated_215_residues
MGVILYRVLLVEDQQIVRQGIKILLEQDKQIKVIGEATNGVEAIEQMMEHTVDVILMDVRMPDMNGIEATRKIKSQWPNV
RILMLTTFNDDEYTLQSLKEGANGFLLKTADGKKLIDAIHSCMRGGLVLHDEVAAKVMPKLLHTSKRELSVQLSERERVI
TALIGSGLTNKEIADELFLTIGTVKNYITSILQKTEQRDRTQLAIFAVRHGITKD
>Mature_214_residues
GVILYRVLLVEDQQIVRQGIKILLEQDKQIKVIGEATNGVEAIEQMMEHTVDVILMDVRMPDMNGIEATRKIKSQWPNVR
ILMLTTFNDDEYTLQSLKEGANGFLLKTADGKKLIDAIHSCMRGGLVLHDEVAAKVMPKLLHTSKRELSVQLSERERVIT
ALIGSGLTNKEIADELFLTIGTVKNYITSILQKTEQRDRTQLAIFAVRHGITKD

Specific function: Member of the two-component regulatory system yfiJ/yfiK [H]

COG id: COG2197

COG function: function code TK; Response regulator containing a CheY-like receiver domain and an HTH DNA-binding domain

Gene ontology:

Cell location: Cytoplasmic [H]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 response regulatory domain [H]

Homologues:

Organism=Escherichia coli, GI1788222, Length=207, Percent_Identity=35.7487922705314, Blast_Score=124, Evalue=4e-30,
Organism=Escherichia coli, GI1787473, Length=202, Percent_Identity=35.1485148514851, Blast_Score=103, Evalue=7e-24,
Organism=Escherichia coli, GI1788521, Length=201, Percent_Identity=29.3532338308458, Blast_Score=99, Evalue=3e-22,
Organism=Escherichia coli, GI1790102, Length=198, Percent_Identity=31.3131313131313, Blast_Score=91, Evalue=5e-20,
Organism=Escherichia coli, GI1786747, Length=187, Percent_Identity=27.807486631016, Blast_Score=89, Evalue=2e-19,
Organism=Escherichia coli, GI1788712, Length=205, Percent_Identity=25.3658536585366, Blast_Score=71, Evalue=5e-14,
Organism=Escherichia coli, GI87082052, Length=109, Percent_Identity=31.1926605504587, Blast_Score=70, Evalue=8e-14,
Organism=Escherichia coli, GI1790437, Length=79, Percent_Identity=36.7088607594937, Blast_Score=62, Evalue=4e-11,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR011006
- InterPro:   IPR001789
- InterPro:   IPR000792
- InterPro:   IPR011991 [H]

Pfam domain/function: PF00196 GerE; PF00072 Response_reg [H]

EC number: NA

Molecular weight: Translated: 24224; Mature: 24093

Theoretical pI: Translated: 7.08; Mature: 7.08

Prosite motif: PS50110 RESPONSE_REGULATORY ; PS00622 HTH_LUXR_1 ; PS50043 HTH_LUXR_2

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.5 %Cys     (Translated Protein)
4.2 %Met     (Translated Protein)
4.7 %Cys+Met (Translated Protein)
0.5 %Cys     (Mature Protein)
3.7 %Met     (Mature Protein)
4.2 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MGVILYRVLLVEDQQIVRQGIKILLEQDKQIKVIGEATNGVEAIEQMMEHTVDVILMDVR
CCHHHHHHHHHCCHHHHHHHHHHHHCCCCEEEEEECCCCCHHHHHHHHHHHHHHEEEEEE
MPDMNGIEATRKIKSQWPNVRILMLTTFNDDEYTLQSLKEGANGFLLKTADGKKLIDAIH
CCCCCCHHHHHHHHHHCCCEEEEEEEEECCCHHHHHHHHCCCCCEEEEECCCHHHHHHHH
SCMRGGLVLHDEVAAKVMPKLLHTSKRELSVQLSERERVITALIGSGLTNKEIADELFLT
HHHHCCEEEHHHHHHHHHHHHHHCCHHHHEEEHHHHHHHHHHHHCCCCCCHHHHHHHHHH
IGTVKNYITSILQKTEQRDRTQLAIFAVRHGITKD
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCC
>Mature Secondary Structure 
GVILYRVLLVEDQQIVRQGIKILLEQDKQIKVIGEATNGVEAIEQMMEHTVDVILMDVR
CHHHHHHHHHCCHHHHHHHHHHHHCCCCEEEEEECCCCCHHHHHHHHHHHHHHEEEEEE
MPDMNGIEATRKIKSQWPNVRILMLTTFNDDEYTLQSLKEGANGFLLKTADGKKLIDAIH
CCCCCCHHHHHHHHHHCCCEEEEEEEEECCCHHHHHHHHCCCCCEEEEECCCHHHHHHHH
SCMRGGLVLHDEVAAKVMPKLLHTSKRELSVQLSERERVITALIGSGLTNKEIADELFLT
HHHHCCEEEHHHHHHHHHHHHHHCCHHHHEEEHHHHHHHHHHHHCCCCCCHHHHHHHHHH
IGTVKNYITSILQKTEQRDRTQLAIFAVRHGITKD
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: DNA [C]

Specific reaction: Protein + DNA = Protein-DNA [C]

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 8973323; 9384377 [H]