Click here to switch to the map view.

The map label for this gene is glnH [H]

Identifier: 23098459

GI number: 23098459

Start: 1047533

End: 1048351

Strand: Direct

Name: glnH [H]

Synonym: OB1004

Alternate gene names: 23098459

Gene position: 1047533-1048351 (Clockwise)

Preceding gene: 23098456

Following gene: 23098460

Centisome position: 28.85

GC content: 36.02

Gene sequence:

>819_bases
ATGAAAAAGAATAAGTGGAGTTTGCTGATTGTATTGTCGGCGTTATTAATTTTCCTTGCGGCTTGCGGATCCGGTGAAGA
GGATTTAGCATCAGGTGAAGGAGAGGATGAAAGTAATAATGAGAGTTCTTATGATTTAGAGGAATCTTATACAGTAGTTA
GTGATAATTCTTTCGTTCCTTTTGAATTTGTAGAAGATGGGGAGTTAACAGGATTTGATATCGAACTAATTAAAGCTATT
GCAGATGAAGCGGGATTTGAAATAGAAGGTGGAGAAATTCAAACAACCAATTTTGATGGAATCATCCCTGGACTTCAGAC
ACAACAATTTGATATTGCTATTGCTGGAATAAGTATTACTGAAGACCGTAAGCAGGTTATCGATTACAGTGAACCTTATT
ATGAGTCCGGTTTGAAAATTGGGGTTTCAGCAGATAATGAAGATATTAATAGTATAGAAGATTTAGAAGGTAAAACAGTT
GCTACTCGTATGGGTTCTACTAGTGCAGATTTTCTTGAAGAAAATACGGAAGATGCGACTATTAATCAATTCGAACAACT
GGATCAGGTTTATTTAGCAGTGGAAAACGGAAGTGCTGATGCAGTGGTTTACGATGCTCCAAATGTTGATTATTATATAT
CCACAACAGGAGATGGATTAAAAACTGTTGGGGAATTATATCAAGCTGAAAATTATGGGATTGCCATTGCAAAAGGAAAT
GAAGACTTAGTTGCTGCGGTTGACGATGCGCTGGCAACGTTAAAAGAAAATGGTAAGTATGATGAAATTTATAATAAGTG
GTTTGGTTCAGAAGAATAA

Upstream 100 bases:

>100_bases
GAAATATAAGTTAAAAGAGAAATTTCTTAATATGTAATTATTTGATACCATTAAATATGTCAGTTTTTGTAGAAATCAAA
CTATTTAGGGGGCAGAAAGA

Downstream 100 bases:

>100_bases
TTTAGTCAGCATTAAGTTTGAGGTAGCTATATAAAATTTTAAAGCTTATAAGGCTTATCCAAAGAAGATAAGCCTTATTT
TATTTAATGAGGTGAAAGAA

Product: glutamine ABC transporter glutamine-binding protein

Products: ADP; phosphate; L-glutamine [Cytoplasm] [C]

Alternate protein names: GlnBP [H]

Number of amino acids: Translated: 272; Mature: 272

Protein sequence:

>272_residues
MKKNKWSLLIVLSALLIFLAACGSGEEDLASGEGEDESNNESSYDLEESYTVVSDNSFVPFEFVEDGELTGFDIELIKAI
ADEAGFEIEGGEIQTTNFDGIIPGLQTQQFDIAIAGISITEDRKQVIDYSEPYYESGLKIGVSADNEDINSIEDLEGKTV
ATRMGSTSADFLEENTEDATINQFEQLDQVYLAVENGSADAVVYDAPNVDYYISTTGDGLKTVGELYQAENYGIAIAKGN
EDLVAAVDDALATLKENGKYDEIYNKWFGSEE

Sequences:

>Translated_272_residues
MKKNKWSLLIVLSALLIFLAACGSGEEDLASGEGEDESNNESSYDLEESYTVVSDNSFVPFEFVEDGELTGFDIELIKAI
ADEAGFEIEGGEIQTTNFDGIIPGLQTQQFDIAIAGISITEDRKQVIDYSEPYYESGLKIGVSADNEDINSIEDLEGKTV
ATRMGSTSADFLEENTEDATINQFEQLDQVYLAVENGSADAVVYDAPNVDYYISTTGDGLKTVGELYQAENYGIAIAKGN
EDLVAAVDDALATLKENGKYDEIYNKWFGSEE
>Mature_272_residues
MKKNKWSLLIVLSALLIFLAACGSGEEDLASGEGEDESNNESSYDLEESYTVVSDNSFVPFEFVEDGELTGFDIELIKAI
ADEAGFEIEGGEIQTTNFDGIIPGLQTQQFDIAIAGISITEDRKQVIDYSEPYYESGLKIGVSADNEDINSIEDLEGKTV
ATRMGSTSADFLEENTEDATINQFEQLDQVYLAVENGSADAVVYDAPNVDYYISTTGDGLKTVGELYQAENYGIAIAKGN
EDLVAAVDDALATLKENGKYDEIYNKWFGSEE

Specific function: Involved in a glutamine-transport system glnHPQ [H]

COG id: COG0834

COG function: function code ET; ABC-type amino acid transport/signal transduction systems, periplasmic component/domain

Gene ontology:

Cell location: Periplasm [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the bacterial solute-binding protein 3 family [H]

Homologues:

Organism=Escherichia coli, GI1787031, Length=231, Percent_Identity=44.5887445887446, Blast_Score=214, Evalue=7e-57,
Organism=Escherichia coli, GI1788228, Length=219, Percent_Identity=31.0502283105023, Blast_Score=129, Evalue=2e-31,
Organism=Escherichia coli, GI1787085, Length=219, Percent_Identity=27.8538812785388, Blast_Score=99, Evalue=2e-22,
Organism=Escherichia coli, GI1787088, Length=228, Percent_Identity=28.0701754385965, Blast_Score=99, Evalue=3e-22,
Organism=Escherichia coli, GI1788649, Length=242, Percent_Identity=25.6198347107438, Blast_Score=81, Evalue=6e-17,
Organism=Escherichia coli, GI1788648, Length=234, Percent_Identity=26.9230769230769, Blast_Score=79, Evalue=3e-16,

Paralogues:

None

Copy number: 1440 Molecules/Cell In: Growth-Phase, Minimal-Media (Based on E. coli). 460 Molecules/Cell In: Stationary Phase, Rich Media (Based on E. coli). [C]

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR015683
- InterPro:   IPR005768
- InterPro:   IPR001638
- InterPro:   IPR018313 [H]

Pfam domain/function: PF00497 SBP_bac_3 [H]

EC number: NA

Molecular weight: Translated: 29652; Mature: 29652

Theoretical pI: Translated: 3.52; Mature: 3.52

Prosite motif: PS00013 PROKAR_LIPOPROTEIN ; PS01039 SBP_BACTERIAL_3

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.4 %Cys     (Translated Protein)
0.7 %Met     (Translated Protein)
1.1 %Cys+Met (Translated Protein)
0.4 %Cys     (Mature Protein)
0.7 %Met     (Mature Protein)
1.1 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MKKNKWSLLIVLSALLIFLAACGSGEEDLASGEGEDESNNESSYDLEESYTVVSDNSFVP
CCCCHHHHHHHHHHHHHHHHHCCCCHHHHHCCCCCCCCCCCCCCCCCCCEEEEECCCCCC
FEFVEDGELTGFDIELIKAIADEAGFEIEGGEIQTTNFDGIIPGLQTQQFDIAIAGISIT
EEEECCCCCCCCHHHHHHHHHHHCCCEEECCEEEECCCCCCCCCCCCCEEEEEEEEEEEC
EDRKQVIDYSEPYYESGLKIGVSADNEDINSIEDLEGKTVATRMGSTSADFLEENTEDAT
CCHHHHHCCCCCHHHCCCEEEEECCCCCCCHHHHCCCCEEEEECCCCHHHHHHCCCCCCH
INQFEQLDQVYLAVENGSADAVVYDAPNVDYYISTTGDGLKTVGELYQAENYGIAIAKGN
HHHHHHHCEEEEEEECCCCCEEEEECCCCCEEEEECCCHHHHHHHHHHCCCCCEEEEECC
EDLVAAVDDALATLKENGKYDEIYNKWFGSEE
CCEEHHHHHHHHHHHHCCCHHHHHHHHCCCCC
>Mature Secondary Structure
MKKNKWSLLIVLSALLIFLAACGSGEEDLASGEGEDESNNESSYDLEESYTVVSDNSFVP
CCCCHHHHHHHHHHHHHHHHHCCCCHHHHHCCCCCCCCCCCCCCCCCCCEEEEECCCCCC
FEFVEDGELTGFDIELIKAIADEAGFEIEGGEIQTTNFDGIIPGLQTQQFDIAIAGISIT
EEEECCCCCCCCHHHHHHHHHHHCCCEEECCEEEECCCCCCCCCCCCCEEEEEEEEEEEC
EDRKQVIDYSEPYYESGLKIGVSADNEDINSIEDLEGKTVATRMGSTSADFLEENTEDAT
CCHHHHHCCCCCHHHCCCEEEEECCCCCCCHHHHCCCCEEEEECCCCHHHHHHCCCCCCH
INQFEQLDQVYLAVENGSADAVVYDAPNVDYYISTTGDGLKTVGELYQAENYGIAIAKGN
HHHHHHHCEEEEEEECCCCCEEEEECCCCCEEEEECCCHHHHHHHHHHCCCCCEEEEECC
EDLVAAVDDALATLKENGKYDEIYNKWFGSEE
CCEEHHHHHHHHHHHHCCCHHHHHHHHCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: ATP; L-glutamine [Periplasm]; H2O [C]

Specific reaction: ATP + L-glutamine [Periplasm] + H2O = ADP + phosphate + L-glutamine [Cytoplasm] [C]

General reaction: Translocates substrate across the membrane [C]

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 11206551; 11258796 [H]