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The map label for this gene is gph [C]

Identifier: 23098387

GI number: 23098387

Start: 981503

End: 982177

Strand: Direct

Name: gph [C]

Synonym: OB0932

Alternate gene names: 23098387

Gene position: 981503-982177 (Clockwise)

Preceding gene: 23098386

Following gene: 23098388

Centisome position: 27.03

GC content: 33.78

Gene sequence:

>675_bases
ATGATAAAGGCTGTCCTATTTGATTTAGATGGAACTTTATTAAATCGAGATGAATCTATAAAAGGGTTCGTAAACCACCA
ATATGAACGACTGAAAAAGTGGGTTGGTCATATACCCAAAGATAAATACATTACAAGATTTTTAGAACTCGACAAGCGTG
GGTATGTTTGGAAAGATAAGGTATATCAGCAATTAATCCAGGAATTTGATATTTCCAAAATGACTTTGGAGGATTTGCTA
CAAGATTACATCAGTGAGTTTAGGTTTAATTGTGTTCCTTTTGATAATTTAATTCAAATGTTAGAAGATTTAAAGAGCAA
AAATGTCCTTTTAGGTATGATTACAAACGGTTATGGGCAATTTCAAATGGATAACATTAAAGCCTTAGGAATTGAGCGAT
ATTTTGATGTGATTTTGGTATCTGAGTGGGAAGGGATAAAAAAACCTGACCCTGAAATTTTTAAAAAGGCTTTAGAAAAA
CTTGACGTACCGCCTGAGCAAAGTATATTCGTTGGGGATCATCCTAAAAATGATGTAAAGGCTGCTCAAAGTATTGGAAT
GAAAGGAATATGGAAAAAGGATCTCCAATGGAACGATGTTGAAGCGGATTTCACAGTTAATGACTTGATGGCACTTCCAT
TAATCGTTAAGAATTTAACTAGAAGAGTGGAGTAA

Upstream 100 bases:

>100_bases
CGGAATCGGGCGCTTATCCGGAATAAAACCTGCGCTCAATATTGGAGAAAAGGGTCATTTTATTAAATAAATATATAAGC
AGTGTAAGGGGGTTGGAAAA

Downstream 100 bases:

>100_bases
AAGCTCACTCTGAAAAGAGTGTAATTTAGATCAAGGGTGATAGAAATTGTTGAATTTAGAAAAAGTGACTAAAGGTAAAG
AAAGCATTTTACATAACCTC

Product: L-2-haloalkanoic acid dehalogenase

Products: Glycolate; Phosphate [C]

Alternate protein names: NA

Number of amino acids: Translated: 224; Mature: 224

Protein sequence:

>224_residues
MIKAVLFDLDGTLLNRDESIKGFVNHQYERLKKWVGHIPKDKYITRFLELDKRGYVWKDKVYQQLIQEFDISKMTLEDLL
QDYISEFRFNCVPFDNLIQMLEDLKSKNVLLGMITNGYGQFQMDNIKALGIERYFDVILVSEWEGIKKPDPEIFKKALEK
LDVPPEQSIFVGDHPKNDVKAAQSIGMKGIWKKDLQWNDVEADFTVNDLMALPLIVKNLTRRVE

Sequences:

>Translated_224_residues
MIKAVLFDLDGTLLNRDESIKGFVNHQYERLKKWVGHIPKDKYITRFLELDKRGYVWKDKVYQQLIQEFDISKMTLEDLL
QDYISEFRFNCVPFDNLIQMLEDLKSKNVLLGMITNGYGQFQMDNIKALGIERYFDVILVSEWEGIKKPDPEIFKKALEK
LDVPPEQSIFVGDHPKNDVKAAQSIGMKGIWKKDLQWNDVEADFTVNDLMALPLIVKNLTRRVE
>Mature_224_residues
MIKAVLFDLDGTLLNRDESIKGFVNHQYERLKKWVGHIPKDKYITRFLELDKRGYVWKDKVYQQLIQEFDISKMTLEDLL
QDYISEFRFNCVPFDNLIQMLEDLKSKNVLLGMITNGYGQFQMDNIKALGIERYFDVILVSEWEGIKKPDPEIFKKALEK
LDVPPEQSIFVGDHPKNDVKAAQSIGMKGIWKKDLQWNDVEADFTVNDLMALPLIVKNLTRRVE

Specific function: Unknown

COG id: COG1011

COG function: function code R; Predicted hydrolase (HAD superfamily)

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the HAD-like hydrolase superfamily [H]

Homologues:

Organism=Escherichia coli, GI1789787, Length=204, Percent_Identity=26.9607843137255, Blast_Score=69, Evalue=2e-13,
Organism=Escherichia coli, GI1790833, Length=102, Percent_Identity=30.3921568627451, Blast_Score=66, Evalue=2e-12,
Organism=Drosophila melanogaster, GI28571108, Length=201, Percent_Identity=27.363184079602, Blast_Score=66, Evalue=2e-11,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR005834
- InterPro:   IPR023214
- InterPro:   IPR011950
- InterPro:   IPR006439
- InterPro:   IPR006402
- InterPro:   IPR005833 [H]

Pfam domain/function: PF00702 Hydrolase [H]

EC number: 3.1.3.18 [C]

Molecular weight: Translated: 26262; Mature: 26262

Theoretical pI: Translated: 5.45; Mature: 5.45

Prosite motif: PS01228 COF_1

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.4 %Cys     (Translated Protein)
3.1 %Met     (Translated Protein)
3.6 %Cys+Met (Translated Protein)
0.4 %Cys     (Mature Protein)
3.1 %Met     (Mature Protein)
3.6 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MIKAVLFDLDGTLLNRDESIKGFVNHQYERLKKWVGHIPKDKYITRFLELDKRGYVWKDK
CCEEEEEECCCCEECCCCHHHHHHHHHHHHHHHHHCCCCCHHHHHHHHHHHCCCCEEHHH
VYQQLIQEFDISKMTLEDLLQDYISEFRFNCVPFDNLIQMLEDLKSKNVLLGMITNGYGQ
HHHHHHHHHCCHHHHHHHHHHHHHHHHCCCCCCHHHHHHHHHHHCCCCEEEEEEECCCCC
FQMDNIKALGIERYFDVILVSEWEGIKKPDPEIFKKALEKLDVPPEQSIFVGDHPKNDVK
EEECCHHHHHHHHHHHHHEEECCCCCCCCCHHHHHHHHHHCCCCCCCCEEECCCCCHHHH
AAQSIGMKGIWKKDLQWNDVEADFTVNDLMALPLIVKNLTRRVE
HHHHCCCCCCCCCCCCCCCCCCCCCHHHHHHHHHHHHHHHHHCC
>Mature Secondary Structure
MIKAVLFDLDGTLLNRDESIKGFVNHQYERLKKWVGHIPKDKYITRFLELDKRGYVWKDK
CCEEEEEECCCCEECCCCHHHHHHHHHHHHHHHHHCCCCCHHHHHHHHHHHCCCCEEHHH
VYQQLIQEFDISKMTLEDLLQDYISEFRFNCVPFDNLIQMLEDLKSKNVLLGMITNGYGQ
HHHHHHHHHCCHHHHHHHHHHHHHHHHCCCCCCHHHHHHHHHHHCCCCEEEEEEECCCCC
FQMDNIKALGIERYFDVILVSEWEGIKKPDPEIFKKALEKLDVPPEQSIFVGDHPKNDVK
EEECCHHHHHHHHHHHHHEEECCCCCCCCCHHHHHHHHHHCCCCCCCCEEECCCCCHHHH
AAQSIGMKGIWKKDLQWNDVEADFTVNDLMALPLIVKNLTRRVE
HHHHCCCCCCCCCCCCCCCCCCCCCHHHHHHHHHHHHHHHHHCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: Mg2+ [C]

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): 0.57 {phosphoglycolate}} [C]

Substrates: D-Glycerate 2-phosphatelyc; H2O [C]

Specific reaction: D-Glycerate 2-phosphatelyc + H2O --> Glycolate + Phosphate [C]

General reaction: Additional information:

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 8688087 [H]