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The map label for this gene is clpE [H]

Identifier: 23098336

GI number: 23098336

Start: 934210

End: 936348

Strand: Direct

Name: clpE [H]

Synonym: OB0881

Alternate gene names: 23098336

Gene position: 934210-936348 (Clockwise)

Preceding gene: 23098335

Following gene: 23098337

Centisome position: 25.73

GC content: 35.2

Gene sequence:

>2139_bases
ATGAAGTGTCAAAACTGTGGTCAAAATGATGCAACAATAAATGCACAAATGCAGATGAATAATCAACGAATGGAAATTCA
TCTATGCCATGAATGTTTCCAAGCTATTCAAGGAAATATGATGAACTCTGACTTTTTCTCTAATTCTCCATTCGGCAATA
TGGATCAAGCATTTGCCAATAACTTTTCTCAAGGAAATGGTGGAAGTGCTACTGGTACACGCACTAAACAAAAAGCAAGT
AAAGGCAATGGCCTTATTGATCAGTTAGCAAAAAACTTAACTGATCAAGCGAGATCAGGAAATGTAGATCCTGTCATTGG
TAGAGATAAAGAAATTAAACGAGTTACGGAAACATTAAATAGAAGAAATAAAAATAACCCAGTTCTTATTGGTGAACCAG
GGGTTGGTAAAACTGCTATTGCGGAAGGACTAGCAGTCAATATAGTAGAAGGTAATGTCCCAGCTAAGTTAATGAATAAA
GAAATTTATTTATTAGATGTAGCTTCATTAGTTGCAAATACTGGAGTTCGAGGTCAATTTGAAGAAAGAATGAAGAAGTT
AGTTGAAGAATTACAATCAAGAAAAGATGTTATCTTATTCATTGACGAAATTCATTTAATCGTTGGAGCTGGAACAGCAG
AAAGTTCACAAATGGATGTTGGTAACTTATTAAAACCAGCATTGGCACGTGGAGATTTACAAATAATTGGTGCTACAACA
CTTAAGGAATATCGACAAATAGAAAAAGACGCTGCACTTGAACGACGCTTGCAGCCAATTATGGTAAACGAACCATCTTT
TGAAGATGCAGTAACCATATTGGAAGGTATCAAAGAGCGATACGAAAAATTCCATGAAGTTCGTTATTCTGAGGATGTCA
TTCGTGCATTTGTTAACTTATCAAGTAGATATATTCAAGATCGTCATTTACCTGATAAAGCCATCGATTTAATGGATGAA
GTAGGTTCTAGATTGAATTTATCCAATGCACAGAAAGACTCTGCTACACTTCAACAACAACTCGACGAAATCATTAAGAA
GAAACAAGAAGCTGCTGATATAGAGGATTATGAAAAAGCAGCAAACTTAAGATATCAAGAAATTCAATTACAAAAACAAT
TGGATAAAGTTGAAGAAGGCGAAAAAGTTTTAGATGTTGATATCAGTGATATCGAGTTGATTGTAGAAGAGAAGACAGGC
ATTCCAGTAACGAAGATGCAAAAAGATGAACAAGAAAAAATGAAAAATCTGTCTGATCAACTAAGTCAGAAGGTGATTGG
CCAGAACGAGGCAGTGCAAAAAGTAGCAAAAGCAATCCGTCGTAGTCGTGCAGGCTTAAAAGCAAAACAACGTCCAATCG
GATCATTCTTATTTGTTGGACCTACAGGTGTGGGTAAAACAGAACTTACGAAAGTACTAGCAGAAGAACTATTTGGTTCC
AGAGATTCTATGGTAAGACTAGATATGAGTGAGTATATGGAGAAACACGCTGTATCTAAAATAATTGGTTCACCACCTGG
TTATGTAGGTCATGAAGAAGCTGGTCAACTTACCGAGCGAATCCGTCGTAATCCATATTCTATTCTTCTACTAGATGAGA
TTGAAAAAGCTCATCCAGATGTACAAAATATGTTCTTACAGATTATGGAAGATGGTCAATTGACTGATTCTCAAGGTAGA
AAAGTAAGCTTTAAAGATACAGTTATCATCATGACAAGTAACGCTGGTACTGGTGTAAAACAGGTTAATGTTGGTTTTAA
TCGTGAAGCCCATGAATCTGTATCCACACTAGAAAACTTGAGCGAATACTTTAAACCAGAATTTCTAAACCGATTTGATG
CTATCGTTAACTTCCAAGAGTTAGCAGAAGAAGACTTGCTACAAATTATTGATCTTATGTTGTCTGAATTAGAAGCAACA
ATAAAAGAAAATAAAATGACAATAACTATATCTGAAGAAGCGAAACAACAGTTAGTAAAATTAGGATATGATACACGTTT
TGGAGCGCGTCCTTTACGTCGTGTTATCCAAGATAAGATTGAAGATCCATTAACTGATCTCATTCTAGAAGGTGAAGATA
TAACTTCTATTAATGTTGATGTCAAAGAAGATGAAATTGTTGTAAACCAAGCTTCTTAA

Upstream 100 bases:

>100_bases
AGATAGTCAAAGTCAAAAATGGTTGTGACATCGCATAGGCGAAAGTCATATATTAAAAAGTAACAGGCTATCTTAACTGT
AATTAGAGGAGGAATGAGAA

Downstream 100 bases:

>100_bases
ATATGAGCAATAGCTAAGCGCTAAGCCAAAGTTGGCTTAGCGTTTTTTTTGTAGGAAATTATAAGATTTTGGGGCTGTGG
ATAAACAACTAAGTTAATTA

Product: ATP-dependent Clp proteinase

Products: NA

Alternate protein names: ATPase ClpE; Heat shock protein HSP1 [H]

Number of amino acids: Translated: 712; Mature: 712

Protein sequence:

>712_residues
MKCQNCGQNDATINAQMQMNNQRMEIHLCHECFQAIQGNMMNSDFFSNSPFGNMDQAFANNFSQGNGGSATGTRTKQKAS
KGNGLIDQLAKNLTDQARSGNVDPVIGRDKEIKRVTETLNRRNKNNPVLIGEPGVGKTAIAEGLAVNIVEGNVPAKLMNK
EIYLLDVASLVANTGVRGQFEERMKKLVEELQSRKDVILFIDEIHLIVGAGTAESSQMDVGNLLKPALARGDLQIIGATT
LKEYRQIEKDAALERRLQPIMVNEPSFEDAVTILEGIKERYEKFHEVRYSEDVIRAFVNLSSRYIQDRHLPDKAIDLMDE
VGSRLNLSNAQKDSATLQQQLDEIIKKKQEAADIEDYEKAANLRYQEIQLQKQLDKVEEGEKVLDVDISDIELIVEEKTG
IPVTKMQKDEQEKMKNLSDQLSQKVIGQNEAVQKVAKAIRRSRAGLKAKQRPIGSFLFVGPTGVGKTELTKVLAEELFGS
RDSMVRLDMSEYMEKHAVSKIIGSPPGYVGHEEAGQLTERIRRNPYSILLLDEIEKAHPDVQNMFLQIMEDGQLTDSQGR
KVSFKDTVIIMTSNAGTGVKQVNVGFNREAHESVSTLENLSEYFKPEFLNRFDAIVNFQELAEEDLLQIIDLMLSELEAT
IKENKMTITISEEAKQQLVKLGYDTRFGARPLRRVIQDKIEDPLTDLILEGEDITSINVDVKEDEIVVNQAS

Sequences:

>Translated_712_residues
MKCQNCGQNDATINAQMQMNNQRMEIHLCHECFQAIQGNMMNSDFFSNSPFGNMDQAFANNFSQGNGGSATGTRTKQKAS
KGNGLIDQLAKNLTDQARSGNVDPVIGRDKEIKRVTETLNRRNKNNPVLIGEPGVGKTAIAEGLAVNIVEGNVPAKLMNK
EIYLLDVASLVANTGVRGQFEERMKKLVEELQSRKDVILFIDEIHLIVGAGTAESSQMDVGNLLKPALARGDLQIIGATT
LKEYRQIEKDAALERRLQPIMVNEPSFEDAVTILEGIKERYEKFHEVRYSEDVIRAFVNLSSRYIQDRHLPDKAIDLMDE
VGSRLNLSNAQKDSATLQQQLDEIIKKKQEAADIEDYEKAANLRYQEIQLQKQLDKVEEGEKVLDVDISDIELIVEEKTG
IPVTKMQKDEQEKMKNLSDQLSQKVIGQNEAVQKVAKAIRRSRAGLKAKQRPIGSFLFVGPTGVGKTELTKVLAEELFGS
RDSMVRLDMSEYMEKHAVSKIIGSPPGYVGHEEAGQLTERIRRNPYSILLLDEIEKAHPDVQNMFLQIMEDGQLTDSQGR
KVSFKDTVIIMTSNAGTGVKQVNVGFNREAHESVSTLENLSEYFKPEFLNRFDAIVNFQELAEEDLLQIIDLMLSELEAT
IKENKMTITISEEAKQQLVKLGYDTRFGARPLRRVIQDKIEDPLTDLILEGEDITSINVDVKEDEIVVNQAS
>Mature_712_residues
MKCQNCGQNDATINAQMQMNNQRMEIHLCHECFQAIQGNMMNSDFFSNSPFGNMDQAFANNFSQGNGGSATGTRTKQKAS
KGNGLIDQLAKNLTDQARSGNVDPVIGRDKEIKRVTETLNRRNKNNPVLIGEPGVGKTAIAEGLAVNIVEGNVPAKLMNK
EIYLLDVASLVANTGVRGQFEERMKKLVEELQSRKDVILFIDEIHLIVGAGTAESSQMDVGNLLKPALARGDLQIIGATT
LKEYRQIEKDAALERRLQPIMVNEPSFEDAVTILEGIKERYEKFHEVRYSEDVIRAFVNLSSRYIQDRHLPDKAIDLMDE
VGSRLNLSNAQKDSATLQQQLDEIIKKKQEAADIEDYEKAANLRYQEIQLQKQLDKVEEGEKVLDVDISDIELIVEEKTG
IPVTKMQKDEQEKMKNLSDQLSQKVIGQNEAVQKVAKAIRRSRAGLKAKQRPIGSFLFVGPTGVGKTELTKVLAEELFGS
RDSMVRLDMSEYMEKHAVSKIIGSPPGYVGHEEAGQLTERIRRNPYSILLLDEIEKAHPDVQNMFLQIMEDGQLTDSQGR
KVSFKDTVIIMTSNAGTGVKQVNVGFNREAHESVSTLENLSEYFKPEFLNRFDAIVNFQELAEEDLLQIIDLMLSELEAT
IKENKMTITISEEAKQQLVKLGYDTRFGARPLRRVIQDKIEDPLTDLILEGEDITSINVDVKEDEIVVNQAS

Specific function: ATPase essential both for efficient CtsR-dependent gene derepression during heat stress and for rerepression. Together with ClpP, degrades the global regulator CtsR after heat shock. Is also involved in disaggregation of heat-denatured proteins. Has thus

COG id: COG0542

COG function: function code O; ATPases with chaperone activity, ATP-binding subunit

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 UVR domain [H]

Homologues:

Organism=Homo sapiens, GI13540606, Length=299, Percent_Identity=34.1137123745819, Blast_Score=181, Evalue=1e-45,
Organism=Escherichia coli, GI1788943, Length=769, Percent_Identity=43.9531859557867, Blast_Score=594, Evalue=1e-171,
Organism=Escherichia coli, GI1787109, Length=622, Percent_Identity=42.2829581993569, Blast_Score=517, Evalue=1e-148,
Organism=Saccharomyces cerevisiae, GI6320464, Length=700, Percent_Identity=41.7142857142857, Blast_Score=532, Evalue=1e-152,
Organism=Saccharomyces cerevisiae, GI6323002, Length=719, Percent_Identity=37.8303198887344, Blast_Score=443, Evalue=1e-125,

Paralogues:

None

Copy number: 560 Molecules/Cell In: Glucose minimal media [C]

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR003593
- InterPro:   IPR013093
- InterPro:   IPR003959
- InterPro:   IPR018368
- InterPro:   IPR001270
- InterPro:   IPR019489
- InterPro:   IPR001943 [H]

Pfam domain/function: PF00004 AAA; PF07724 AAA_2; PF10431 ClpB_D2-small; PF02151 UVR [H]

EC number: NA

Molecular weight: Translated: 79831; Mature: 79831

Theoretical pI: Translated: 4.84; Mature: 4.84

Prosite motif: PS50151 UVR ; PS00871 CLPAB_2

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.6 %Cys     (Translated Protein)
3.1 %Met     (Translated Protein)
3.7 %Cys+Met (Translated Protein)
0.6 %Cys     (Mature Protein)
3.1 %Met     (Mature Protein)
3.7 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MKCQNCGQNDATINAQMQMNNQRMEIHLCHECFQAIQGNMMNSDFFSNSPFGNMDQAFAN
CCCCCCCCCCCEEEEEEEECCCHHHHHHHHHHHHHHHCCCCCCCCCCCCCCCCHHHHHHC
NFSQGNGGSATGTRTKQKASKGNGLIDQLAKNLTDQARSGNVDPVIGRDKEIKRVTETLN
CCCCCCCCCCCCCCHHHHHHCCCCHHHHHHHHHHHHHHCCCCCCCCCCCHHHHHHHHHHH
RRNKNNPVLIGEPGVGKTAIAEGLAVNIVEGNVPAKLMNKEIYLLDVASLVANTGVRGQF
HCCCCCCEEEECCCCCHHHHHCCCEEEEEECCCCHHHHCCCEEEEEHHHHHHCCCCCCHH
EERMKKLVEELQSRKDVILFIDEIHLIVGAGTAESSQMDVGNLLKPALARGDLQIIGATT
HHHHHHHHHHHHCCCCEEEEEEEEEEEEECCCCCCCCCCHHHHHHHHHCCCCEEEEEHHH
LKEYRQIEKDAALERRLQPIMVNEPSFEDAVTILEGIKERYEKFHEVRYSEDVIRAFVNL
HHHHHHHHHHHHHHHCCCCEEECCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
SSRYIQDRHLPDKAIDLMDEVGSRLNLSNAQKDSATLQQQLDEIIKKKQEAADIEDYEKA
HHHHHHHCCCCHHHHHHHHHHCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHCCHHHHHHH
ANLRYQEIQLQKQLDKVEEGEKVLDVDISDIELIVEEKTGIPVTKMQKDEQEKMKNLSDQ
HCCHHHHHHHHHHHHHHHCCCEEEECCCCHHEEEEECCCCCCHHHCCCHHHHHHHHHHHH
LSQKVIGQNEAVQKVAKAIRRSRAGLKAKQRPIGSFLFVGPTGVGKTELTKVLAEELFGS
HHHHHHCCCHHHHHHHHHHHHHHCCCCHHCCCCCCEEEECCCCCCHHHHHHHHHHHHHCC
RDSMVRLDMSEYMEKHAVSKIIGSPPGYVGHEEAGQLTERIRRNPYSILLLDEIEKAHPD
CCCEEEECHHHHHHHHHHHHHHCCCCCCCCCHHHHHHHHHHHCCCCEEEEEHHHHHHCCC
VQNMFLQIMEDGQLTDSQGRKVSFKDTVIIMTSNAGTGVKQVNVGFNREAHESVSTLENL
HHHHHHHHHHCCCCCCCCCCEEEECCEEEEEECCCCCCCEEEECCCCCHHHHHHHHHHHH
SEYFKPEFLNRFDAIVNFQELAEEDLLQIIDLMLSELEATIKENKMTITISEEAKQQLVK
HHHHCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCEEEEECHHHHHHHHH
LGYDTRFGARPLRRVIQDKIEDPLTDLILEGEDITSINVDVKEDEIVVNQAS
HCCCCCCCHHHHHHHHHHHHHCCHHHHHCCCCCEEEEEEEECCCEEEEECCC
>Mature Secondary Structure
MKCQNCGQNDATINAQMQMNNQRMEIHLCHECFQAIQGNMMNSDFFSNSPFGNMDQAFAN
CCCCCCCCCCCEEEEEEEECCCHHHHHHHHHHHHHHHCCCCCCCCCCCCCCCCHHHHHHC
NFSQGNGGSATGTRTKQKASKGNGLIDQLAKNLTDQARSGNVDPVIGRDKEIKRVTETLN
CCCCCCCCCCCCCCHHHHHHCCCCHHHHHHHHHHHHHHCCCCCCCCCCCHHHHHHHHHHH
RRNKNNPVLIGEPGVGKTAIAEGLAVNIVEGNVPAKLMNKEIYLLDVASLVANTGVRGQF
HCCCCCCEEEECCCCCHHHHHCCCEEEEEECCCCHHHHCCCEEEEEHHHHHHCCCCCCHH
EERMKKLVEELQSRKDVILFIDEIHLIVGAGTAESSQMDVGNLLKPALARGDLQIIGATT
HHHHHHHHHHHHCCCCEEEEEEEEEEEEECCCCCCCCCCHHHHHHHHHCCCCEEEEEHHH
LKEYRQIEKDAALERRLQPIMVNEPSFEDAVTILEGIKERYEKFHEVRYSEDVIRAFVNL
HHHHHHHHHHHHHHHCCCCEEECCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
SSRYIQDRHLPDKAIDLMDEVGSRLNLSNAQKDSATLQQQLDEIIKKKQEAADIEDYEKA
HHHHHHHCCCCHHHHHHHHHHCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHCCHHHHHHH
ANLRYQEIQLQKQLDKVEEGEKVLDVDISDIELIVEEKTGIPVTKMQKDEQEKMKNLSDQ
HCCHHHHHHHHHHHHHHHCCCEEEECCCCHHEEEEECCCCCCHHHCCCHHHHHHHHHHHH
LSQKVIGQNEAVQKVAKAIRRSRAGLKAKQRPIGSFLFVGPTGVGKTELTKVLAEELFGS
HHHHHHCCCHHHHHHHHHHHHHHCCCCHHCCCCCCEEEECCCCCCHHHHHHHHHHHHHCC
RDSMVRLDMSEYMEKHAVSKIIGSPPGYVGHEEAGQLTERIRRNPYSILLLDEIEKAHPD
CCCEEEECHHHHHHHHHHHHHHCCCCCCCCCHHHHHHHHHHHCCCCEEEEEHHHHHHCCC
VQNMFLQIMEDGQLTDSQGRKVSFKDTVIIMTSNAGTGVKQVNVGFNREAHESVSTLENL
HHHHHHHHHHCCCCCCCCCCEEEECCEEEEEECCCCCCCEEEECCCCCHHHHHHHHHHHH
SEYFKPEFLNRFDAIVNFQELAEEDLLQIIDLMLSELEATIKENKMTITISEEAKQQLVK
HHHHCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCEEEEECHHHHHHHHH
LGYDTRFGARPLRRVIQDKIEDPLTDLILEGEDITSINVDVKEDEIVVNQAS
HCCCCCCCHHHHHHHHHHHHHCCHHHHHCCCCCEEEEEEEECCCEEEEECCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: Hydrolase; Acting on peptide bonds (Peptidases); Serine endopeptidases [C]

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 9384377 [H]