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The map label for this gene is cwlM [H]

Identifier: 23098309

GI number: 23098309

Start: 904596

End: 905390

Strand: Direct

Name: cwlM [H]

Synonym: OB0854

Alternate gene names: 23098309

Gene position: 904596-905390 (Clockwise)

Preceding gene: 23098308

Following gene: 23098310

Centisome position: 24.92

GC content: 37.23

Gene sequence:

>795_bases
ATGAAGCGAATTTTTATTGATCCTGGTCATGGAGGAAATGACCCTGGTGCAATGGGAAATGGATTACAGGAAAAAGATAT
CAACTTAGATATTGCGCTTCGTATGAAAGAATACTTGTTACAAACATTTGGTGGACATGAAGTACGATTGTCTCGTGAAA
CAGATAAAACGGTCTCTCTATCGCAAAGAACGACAATGGCAAATAATTGGCAAGCTGATTATTTAGTTTCTATTCATATT
AATGCTGGTGGAGGCACTGGGTTTGAATCATATATTTTTAATGGCATATATTCTGGAAAGGCGGAAACTCATCGCTTGCG
TCAACTAGTTCATCAATCGATTGTCAATGAGACAGGATATTATGATAGGGGCATGAAAGAAGCGAATTTTCACATGCTTC
GTGAATCGCAAATGCCCGCGGTGCTAACGGAGAATGGATTTATTGATTATAGAGAAGACGCTAACAAACTTAAATCTTCT
TCCTTTCTTCAATCGATAGCAAAGAGTCACGCAACTGGAGTTGCCGATGTACTTGAACTGCCAGACGGTGAGGGTGCATC
TCAAGGGTATGTAGAAATTCTCGCAGATTCTTTATGGACATATAATAGTCCAGAATGGGAAGATAAGGCAGTTATTGTTC
ATAAAGGAGAAGTATTCACAGTAATAAAAGATAAGTTTCCTGTTGGTGATGGACATATGTATCAAATTAAAAGTGGTTTA
TATATTACAGCAAATAAAAATTATGTGCGTTATTATACTCGCTCAACTTCCAAAGTAAAATCCGTCTTAAGTTAA

Upstream 100 bases:

>100_bases
TTCAAAAATAATTATGTGACATACAAAGGCAAAAAACAACATCAAGTACTTGTGGAACATCAATTAGCAAAATAATAAAA
TATGGACAAGGAGGATAAGT

Downstream 100 bases:

>100_bases
ATATTTCATTTCATTACCATAGAAAAATACCTACGTATAAGATAAACTATACGTTAGGTATTTTTTTATTGAATGGGGAG
AGTCAAAATGAAGCTATTAG

Product: N-acetylmuramoyl-L-alanine amidase

Products: NA

Alternate protein names: Autolysin; Cell wall hydrolase [H]

Number of amino acids: Translated: 264; Mature: 264

Protein sequence:

>264_residues
MKRIFIDPGHGGNDPGAMGNGLQEKDINLDIALRMKEYLLQTFGGHEVRLSRETDKTVSLSQRTTMANNWQADYLVSIHI
NAGGGTGFESYIFNGIYSGKAETHRLRQLVHQSIVNETGYYDRGMKEANFHMLRESQMPAVLTENGFIDYREDANKLKSS
SFLQSIAKSHATGVADVLELPDGEGASQGYVEILADSLWTYNSPEWEDKAVIVHKGEVFTVIKDKFPVGDGHMYQIKSGL
YITANKNYVRYYTRSTSKVKSVLS

Sequences:

>Translated_264_residues
MKRIFIDPGHGGNDPGAMGNGLQEKDINLDIALRMKEYLLQTFGGHEVRLSRETDKTVSLSQRTTMANNWQADYLVSIHI
NAGGGTGFESYIFNGIYSGKAETHRLRQLVHQSIVNETGYYDRGMKEANFHMLRESQMPAVLTENGFIDYREDANKLKSS
SFLQSIAKSHATGVADVLELPDGEGASQGYVEILADSLWTYNSPEWEDKAVIVHKGEVFTVIKDKFPVGDGHMYQIKSGL
YITANKNYVRYYTRSTSKVKSVLS
>Mature_264_residues
MKRIFIDPGHGGNDPGAMGNGLQEKDINLDIALRMKEYLLQTFGGHEVRLSRETDKTVSLSQRTTMANNWQADYLVSIHI
NAGGGTGFESYIFNGIYSGKAETHRLRQLVHQSIVNETGYYDRGMKEANFHMLRESQMPAVLTENGFIDYREDANKLKSS
SFLQSIAKSHATGVADVLELPDGEGASQGYVEILADSLWTYNSPEWEDKAVIVHKGEVFTVIKDKFPVGDGHMYQIKSGL
YITANKNYVRYYTRSTSKVKSVLS

Specific function: Hydrolyzes the cell wall of M.luteus more efficiently than that of B.licheniformis and B.subtilis. The C-terminal region, including the repeats, determines substrate specificity [H]

COG id: COG0860

COG function: function code M; N-acetylmuramoyl-L-alanine amidase

Gene ontology:

Cell location: Secreted (Probable). Note=Accumulates in cells as inclusion bodies. May be secreted by a mechanism different from the normal export system [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the N-acetylmuramoyl-L-alanine amidase 3 family [H]

Homologues:

None

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR002508
- InterPro:   IPR007730 [H]

Pfam domain/function: PF01520 Amidase_3; PF05036 SPOR [H]

EC number: =3.5.1.28 [H]

Molecular weight: Translated: 29574; Mature: 29574

Theoretical pI: Translated: 6.81; Mature: 6.81

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.0 %Cys     (Translated Protein)
3.0 %Met     (Translated Protein)
3.0 %Cys+Met (Translated Protein)
0.0 %Cys     (Mature Protein)
3.0 %Met     (Mature Protein)
3.0 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MKRIFIDPGHGGNDPGAMGNGLQEKDINLDIALRMKEYLLQTFGGHEVRLSRETDKTVSL
CCEEEECCCCCCCCCCCCCCCCCCCCCCEEEEHHHHHHHHHHCCCCEEEEECCCCCEEEH
SQRTTMANNWQADYLVSIHINAGGGTGFESYIFNGIYSGKAETHRLRQLVHQSIVNETGY
HHHHHCCCCCCCEEEEEEEEECCCCCCHHHHHHHHHCCCCHHHHHHHHHHHHHHHHCCCC
YDRGMKEANFHMLRESQMPAVLTENGFIDYREDANKLKSSSFLQSIAKSHATGVADVLEL
CCCCCCCCHHHHHHHCCCCEEEECCCCEECHHHHHHHHHHHHHHHHHHHHCCCHHHHHCC
PDGEGASQGYVEILADSLWTYNSPEWEDKAVIVHKGEVFTVIKDKFPVGDGHMYQIKSGL
CCCCCCCCCHHHHHHHHHHCCCCCCCCCCEEEEECCCEEEEEECCCCCCCCEEEEEECCE
YITANKNYVRYYTRSTSKVKSVLS
EEEECCCEEEEEECCHHHHHHHCC
>Mature Secondary Structure
MKRIFIDPGHGGNDPGAMGNGLQEKDINLDIALRMKEYLLQTFGGHEVRLSRETDKTVSL
CCEEEECCCCCCCCCCCCCCCCCCCCCCEEEEHHHHHHHHHHCCCCEEEEECCCCCEEEH
SQRTTMANNWQADYLVSIHINAGGGTGFESYIFNGIYSGKAETHRLRQLVHQSIVNETGY
HHHHHCCCCCCCEEEEEEEEECCCCCCHHHHHHHHHCCCCHHHHHHHHHHHHHHHHCCCC
YDRGMKEANFHMLRESQMPAVLTENGFIDYREDANKLKSSSFLQSIAKSHATGVADVLEL
CCCCCCCCHHHHHHHCCCCEEEECCCCEECHHHHHHHHHHHHHHHHHHHHCCCHHHHHCC
PDGEGASQGYVEILADSLWTYNSPEWEDKAVIVHKGEVFTVIKDKFPVGDGHMYQIKSGL
CCCCCCCCCHHHHHHHHHHCCCCCCCCCCEEEEECCCEEEEEECCCCCCCCEEEEEECCE
YITANKNYVRYYTRSTSKVKSVLS
EEEECCCEEEEEECCHHHHHHHCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 1495475 [H]