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The map label for this gene is licH [H]

Identifier: 23098266

GI number: 23098266

Start: 863948

End: 865270

Strand: Reverse

Name: licH [H]

Synonym: OB0811

Alternate gene names: 23098266

Gene position: 865270-863948 (Counterclockwise)

Preceding gene: 23098272

Following gene: 23098262

Centisome position: 23.83

GC content: 37.79

Gene sequence:

>1323_bases
ATGAACGGAATAAAAATTGTCACTATAGGTGGAGGGTCAAGCTACACCCCTGAACTTATAGAAGGATTTATTAAGCGTTA
TGATGAATTACCCGTCAATGAAATCTGGCTTGTAGATATTGAAGCAGGAAAAGAAAAGTTAGAAATTGTAGGTGATCTAG
CAAAAAGAATGGTAAAAGAAGCAAATGTCCCTATCAATATTCATCTGACACTTGACCGAAAAGAAGCACTTAAGAACGCA
GATTTTGTAACCACACAATTTCGGGTTGGCCAACTGGAAGCAAGGGCAAAAGATGAGAGAATACCTTTAGAATATGGAGT
AATTGGTCAAGAAACAAATGGACCAGGTGGGTTATTTAAAGGACTTCGAACGATTCCTGTCATTCTAGATATCGTCAAAG
ATATGGAAGAACAATGTCCAAATGCTTGGTTAATTAACTTCACAAATCCTGCCGGAATGGTCACTGAAGCTGTGCTAAGA
CATAGCAATTGGAAAAAAGTTATTGGTCTGTGCAATGTTCCTATAGGGATGAAGATGATGGTTGCAAACTTTTTAGAAGT
GGAGCCACATCGAGTAGAAATAGACTTTGCTGGACTCAACCATATGGTCTTCGGTTTACATATTTATGTAGACGGAAAAG
AAACTACTTCTGATGTATTAAAACGAATGACAGAGACTAATTCCACTGGTACTATGGAAAATATTGTTGACCTGGGATGG
GAACCCTCCTTTATTGAAGCCCTAGGAGTCCTTCCATGTCCATACCATCGTTATTATTTTAAAACAGATGAGATGCTTGA
CAATGAAAAAGAAAATGCCGTATCAAGCGGTACTCGTGCAGAGGTAGTAAAAAAATTAGAAGATGAGTTATTTGAGCTGT
ATAAAGACCCGACTCTATCAATTAAACCACCTCAATTAGAGAAGCGAGGTGGAGCATACTATAGTGAGGCTGCATGTAGT
TTAATAGATTCTATTTATAATGATCGGAGAGATATTCAGCCAGTAAATACAATAAATCGTGGTGCAATTCAAAGTATCCC
TTATGAGTCTGCGGTAGAAATAAGCTGTGTGATTACAAAAGAAGGTCCAAAACCAATTATCATGGATGAGCTTCCTGTAG
CAGTTCAAGGGTTGGTCAGTCAGATCAAGTCTTTCGAAAGGATAGCGGCAGATTCCGCTGTTTCTGGTGATTATAATAAA
GCACTTTTAGCAATGACGATTAATCCCCTTGTTCCATCAGATACGATCGCTAAGAAAATTCTCAATGAAATGTTAGAAGC
ACATAAAGATTATCTGCCCAACTTTTTTGAAAAAGTCCAATAA

Upstream 100 bases:

>100_bases
AAAACATCATTCATTCACTCGCCATTTCTCTTTAGTATGTAAGTTTATTATAAATCATATTTTGATAATTTTAAATTATT
AGAAGGGATGTTTTCATTTT

Downstream 100 bases:

>100_bases
CAACGAAAAGTTCCGATCATTTGAATCTAGTGATCGGAACTTTCTCTTATTATAGAAATCTATTAATACGCAAGCGTATT
CCAAGCAGTAATAGATTCAA

Product: 6-phospho-beta-glucosidase

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 440; Mature: 440

Protein sequence:

>440_residues
MNGIKIVTIGGGSSYTPELIEGFIKRYDELPVNEIWLVDIEAGKEKLEIVGDLAKRMVKEANVPINIHLTLDRKEALKNA
DFVTTQFRVGQLEARAKDERIPLEYGVIGQETNGPGGLFKGLRTIPVILDIVKDMEEQCPNAWLINFTNPAGMVTEAVLR
HSNWKKVIGLCNVPIGMKMMVANFLEVEPHRVEIDFAGLNHMVFGLHIYVDGKETTSDVLKRMTETNSTGTMENIVDLGW
EPSFIEALGVLPCPYHRYYFKTDEMLDNEKENAVSSGTRAEVVKKLEDELFELYKDPTLSIKPPQLEKRGGAYYSEAACS
LIDSIYNDRRDIQPVNTINRGAIQSIPYESAVEISCVITKEGPKPIIMDELPVAVQGLVSQIKSFERIAADSAVSGDYNK
ALLAMTINPLVPSDTIAKKILNEMLEAHKDYLPNFFEKVQ

Sequences:

>Translated_440_residues
MNGIKIVTIGGGSSYTPELIEGFIKRYDELPVNEIWLVDIEAGKEKLEIVGDLAKRMVKEANVPINIHLTLDRKEALKNA
DFVTTQFRVGQLEARAKDERIPLEYGVIGQETNGPGGLFKGLRTIPVILDIVKDMEEQCPNAWLINFTNPAGMVTEAVLR
HSNWKKVIGLCNVPIGMKMMVANFLEVEPHRVEIDFAGLNHMVFGLHIYVDGKETTSDVLKRMTETNSTGTMENIVDLGW
EPSFIEALGVLPCPYHRYYFKTDEMLDNEKENAVSSGTRAEVVKKLEDELFELYKDPTLSIKPPQLEKRGGAYYSEAACS
LIDSIYNDRRDIQPVNTINRGAIQSIPYESAVEISCVITKEGPKPIIMDELPVAVQGLVSQIKSFERIAADSAVSGDYNK
ALLAMTINPLVPSDTIAKKILNEMLEAHKDYLPNFFEKVQ
>Mature_440_residues
MNGIKIVTIGGGSSYTPELIEGFIKRYDELPVNEIWLVDIEAGKEKLEIVGDLAKRMVKEANVPINIHLTLDRKEALKNA
DFVTTQFRVGQLEARAKDERIPLEYGVIGQETNGPGGLFKGLRTIPVILDIVKDMEEQCPNAWLINFTNPAGMVTEAVLR
HSNWKKVIGLCNVPIGMKMMVANFLEVEPHRVEIDFAGLNHMVFGLHIYVDGKETTSDVLKRMTETNSTGTMENIVDLGW
EPSFIEALGVLPCPYHRYYFKTDEMLDNEKENAVSSGTRAEVVKKLEDELFELYKDPTLSIKPPQLEKRGGAYYSEAACS
LIDSIYNDRRDIQPVNTINRGAIQSIPYESAVEISCVITKEGPKPIIMDELPVAVQGLVSQIKSFERIAADSAVSGDYNK
ALLAMTINPLVPSDTIAKKILNEMLEAHKDYLPNFFEKVQ

Specific function: Hydrolyzes phospho-beta-glucosides (Probable) [H]

COG id: COG1486

COG function: function code G; Alpha-galactosidases/6-phospho-beta-glucosidases, family 4 of glycosyl hydrolases

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the glycosyl hydrolase 4 family [H]

Homologues:

Organism=Escherichia coli, GI1788029, Length=436, Percent_Identity=54.5871559633027, Blast_Score=483, Evalue=1e-137,
Organism=Escherichia coli, GI1790560, Length=467, Percent_Identity=24.1970021413276, Blast_Score=126, Evalue=2e-30,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR019802
- InterPro:   IPR001088
- InterPro:   IPR022616
- InterPro:   IPR015955
- InterPro:   IPR016040 [H]

Pfam domain/function: PF02056 Glyco_hydro_4; PF11975 Glyco_hydro_4C [H]

EC number: =3.2.1.86 [H]

Molecular weight: Translated: 49193; Mature: 49193

Theoretical pI: Translated: 4.77; Mature: 4.77

Prosite motif: PS01324 GLYCOSYL_HYDROL_F4

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.1 %Cys     (Translated Protein)
3.2 %Met     (Translated Protein)
4.3 %Cys+Met (Translated Protein)
1.1 %Cys     (Mature Protein)
3.2 %Met     (Mature Protein)
4.3 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MNGIKIVTIGGGSSYTPELIEGFIKRYDELPVNEIWLVDIEAGKEKLEIVGDLAKRMVKE
CCCEEEEEECCCCCCCHHHHHHHHHHHHCCCCCCEEEEEECCCHHHHHHHHHHHHHHHHH
ANVPINIHLTLDRKEALKNADFVTTQFRVGQLEARAKDERIPLEYGVIGQETNGPGGLFK
CCCCEEEEEEECHHHHHCCCCEEEEEEEECHHHHHCCCCCCCEEECCEECCCCCCHHHHH
GLRTIPVILDIVKDMEEQCPNAWLINFTNPAGMVTEAVLRHSNWKKVIGLCNVPIGMKMM
HHHHHHHHHHHHHHHHHHCCCCEEEEECCCHHHHHHHHHHCCCCHHHHEECCCCCCHHHH
VANFLEVEPHRVEIDFAGLNHMVFGLHIYVDGKETTSDVLKRMTETNSTGTMENIVDLGW
HHHHHHCCCCEEEEEECCCCEEEEEEEEEECCCCCHHHHHHHHHHCCCCCCHHHHHHCCC
EPSFIEALGVLPCPYHRYYFKTDEMLDNEKENAVSSGTRAEVVKKLEDELFELYKDPTLS
CCHHHHHHCCCCCCCCCEEEECHHHHCCCHHHHHCCCHHHHHHHHHHHHHHHHHCCCCCC
IKPPQLEKRGGAYYSEAACSLIDSIYNDRRDIQPVNTINRGAIQSIPYESAVEISCVITK
CCCCCHHHCCCCHHHHHHHHHHHHHHCCCCCCCCCCCCCCCHHHCCCCCCCEEEEEEEEC
EGPKPIIMDELPVAVQGLVSQIKSFERIAADSAVSGDYNKALLAMTINPLVPSDTIAKKI
CCCCCEEECCCCHHHHHHHHHHHHHHHHHHCCCCCCCCCCEEEEEEECCCCCCHHHHHHH
LNEMLEAHKDYLPNFFEKVQ
HHHHHHHHHHHHHHHHHHCC
>Mature Secondary Structure
MNGIKIVTIGGGSSYTPELIEGFIKRYDELPVNEIWLVDIEAGKEKLEIVGDLAKRMVKE
CCCEEEEEECCCCCCCHHHHHHHHHHHHCCCCCCEEEEEECCCHHHHHHHHHHHHHHHHH
ANVPINIHLTLDRKEALKNADFVTTQFRVGQLEARAKDERIPLEYGVIGQETNGPGGLFK
CCCCEEEEEEECHHHHHCCCCEEEEEEEECHHHHHCCCCCCCEEECCEECCCCCCHHHHH
GLRTIPVILDIVKDMEEQCPNAWLINFTNPAGMVTEAVLRHSNWKKVIGLCNVPIGMKMM
HHHHHHHHHHHHHHHHHHCCCCEEEEECCCHHHHHHHHHHCCCCHHHHEECCCCCCHHHH
VANFLEVEPHRVEIDFAGLNHMVFGLHIYVDGKETTSDVLKRMTETNSTGTMENIVDLGW
HHHHHHCCCCEEEEEECCCCEEEEEEEEEECCCCCHHHHHHHHHHCCCCCCHHHHHHCCC
EPSFIEALGVLPCPYHRYYFKTDEMLDNEKENAVSSGTRAEVVKKLEDELFELYKDPTLS
CCHHHHHHCCCCCCCCCEEEECHHHHCCCHHHHHCCCHHHHHHHHHHHHHHHHHCCCCCC
IKPPQLEKRGGAYYSEAACSLIDSIYNDRRDIQPVNTINRGAIQSIPYESAVEISCVITK
CCCCCHHHCCCCHHHHHHHHHHHHHHCCCCCCCCCCCCCCCHHHCCCCCCCEEEEEEEEC
EGPKPIIMDELPVAVQGLVSQIKSFERIAADSAVSGDYNKALLAMTINPLVPSDTIAKKI
CCCCCEEECCCCHHHHHHHHHHHHHHHHHHCCCCCCCCCCEEEEEEECCCCCCHHHHHHH
LNEMLEAHKDYLPNFFEKVQ
HHHHHHHHHHHHHHHHHHCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 8990303; 9384377; 8969509 [H]