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The map label for this gene is dus2 [H]

Identifier: 23098238

GI number: 23098238

Start: 839647

End: 840627

Strand: Direct

Name: dus2 [H]

Synonym: OB0783

Alternate gene names: 23098238

Gene position: 839647-840627 (Clockwise)

Preceding gene: 23098236

Following gene: 23098239

Centisome position: 23.13

GC content: 39.45

Gene sequence:

>981_bases
ATGATAGATAATTTTTGGCGTGATTTACCACGTCCGTTTTTTGTACTTGCACCAATGGAAGATGTGACGGATGTTGTTTT
CCGTCATGTTGTGACAGAAGCAGCTAGGCCGGATGTGTTTTTTACGGAGTTTACAAATTCAGAGAGCTATTGTCATCCAG
ATGGTCGCCAAAGTGTACGCGGACGTCTGACATTTACGGAAGATGAGCAACCGATTGTCGCTCATATTTGGGGAGATAAA
CCAGAATACTTCCGACAAATGAGTATTGGTATGGCTGAAATGGGTTTTAAAGGTCTAGATATTAATATGGGATGCCCTGT
AGCAAATGTAGCAGGAAATGGAAAAGGTTCTGGTCTGATTAATCGTCCAGAGATTGCTGCAGAATTAATACAGGCAGCAA
AAGCTGGAGGTTTGCCTGTAAGTGTGAAGACAAGGCTTGGTTACACGTATATTGATGAATGGAAAGAATGGCTGAAGCAT
GTGTTGGAACAAGATATTGTCAATTTATCGATTCATCTTCGTACAAGAAAAGAAATGAGCAAAGTAGACGCTCATTGGGA
ACTTATTCCGGAGATTAAAAAACTTCGTGATGAAATTGCACCAGATACTCTATTAACGATCAATGGGGATATTCCTGATC
GTCAAACGGGTTTGAAACTCGTGGAGGAGTACGGCGTTGATGGAGTAATGATTGGACGAGGTGTGTTTAAGAATCCTTTT
GCTTTTGAAAAGGAGCAGAAAGAGCATAGCAGTCAAGAAATGCTTGATCTTTTACGTTTACATTTGGACCTTCATGATAA
ATATGACGAGTTAGAATCTCGTTCATTTAAACCACTTCATCGTTTCTTCAAGATTTATGTTAAAGGATTTAGGGGTGCAA
GTGAATTAAGAAATCAAATGATGAATACAAAATCCACTGCGGAAGTGCGCGCATTACTAGATAACTTTGAGATAGAAAAT
ACGATTGAAGTTGATCAGTAG

Upstream 100 bases:

>100_bases
TTGTTTAACTTACGGAAAAATGATATAAGTGTAAAAGAAGAAAAGATATATTGGGATAAGGGTTATTTATTACCCACAAT
GAGAGGATTGAAATGTAACA

Downstream 100 bases:

>100_bases
AAGTAATCTACTGATCACAAAAAAGGCTGAAAACATTATTCCAGCCTTTTTTGCTATATACAACGCTTGTAGAGTGAAAC
TGTTAATAGCAGTTTGAATT

Product: hypothetical protein

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 326; Mature: 326

Protein sequence:

>326_residues
MIDNFWRDLPRPFFVLAPMEDVTDVVFRHVVTEAARPDVFFTEFTNSESYCHPDGRQSVRGRLTFTEDEQPIVAHIWGDK
PEYFRQMSIGMAEMGFKGLDINMGCPVANVAGNGKGSGLINRPEIAAELIQAAKAGGLPVSVKTRLGYTYIDEWKEWLKH
VLEQDIVNLSIHLRTRKEMSKVDAHWELIPEIKKLRDEIAPDTLLTINGDIPDRQTGLKLVEEYGVDGVMIGRGVFKNPF
AFEKEQKEHSSQEMLDLLRLHLDLHDKYDELESRSFKPLHRFFKIYVKGFRGASELRNQMMNTKSTAEVRALLDNFEIEN
TIEVDQ

Sequences:

>Translated_326_residues
MIDNFWRDLPRPFFVLAPMEDVTDVVFRHVVTEAARPDVFFTEFTNSESYCHPDGRQSVRGRLTFTEDEQPIVAHIWGDK
PEYFRQMSIGMAEMGFKGLDINMGCPVANVAGNGKGSGLINRPEIAAELIQAAKAGGLPVSVKTRLGYTYIDEWKEWLKH
VLEQDIVNLSIHLRTRKEMSKVDAHWELIPEIKKLRDEIAPDTLLTINGDIPDRQTGLKLVEEYGVDGVMIGRGVFKNPF
AFEKEQKEHSSQEMLDLLRLHLDLHDKYDELESRSFKPLHRFFKIYVKGFRGASELRNQMMNTKSTAEVRALLDNFEIEN
TIEVDQ
>Mature_326_residues
MIDNFWRDLPRPFFVLAPMEDVTDVVFRHVVTEAARPDVFFTEFTNSESYCHPDGRQSVRGRLTFTEDEQPIVAHIWGDK
PEYFRQMSIGMAEMGFKGLDINMGCPVANVAGNGKGSGLINRPEIAAELIQAAKAGGLPVSVKTRLGYTYIDEWKEWLKH
VLEQDIVNLSIHLRTRKEMSKVDAHWELIPEIKKLRDEIAPDTLLTINGDIPDRQTGLKLVEEYGVDGVMIGRGVFKNPF
AFEKEQKEHSSQEMLDLLRLHLDLHDKYDELESRSFKPLHRFFKIYVKGFRGASELRNQMMNTKSTAEVRALLDNFEIEN
TIEVDQ

Specific function: Catalyzes the synthesis of dihydrouridine, a modified base found in the D-loop of most tRNAs [H]

COG id: COG0042

COG function: function code J; tRNA-dihydrouridine synthase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the dus family [H]

Homologues:

Organism=Homo sapiens, GI40807366, Length=328, Percent_Identity=21.6463414634146, Blast_Score=69, Evalue=9e-12,
Organism=Escherichia coli, GI1788462, Length=240, Percent_Identity=29.5833333333333, Blast_Score=93, Evalue=3e-20,
Organism=Escherichia coli, GI1789660, Length=287, Percent_Identity=27.1777003484321, Blast_Score=82, Evalue=4e-17,
Organism=Escherichia coli, GI145693211, Length=243, Percent_Identity=24.2798353909465, Blast_Score=77, Evalue=1e-15,
Organism=Caenorhabditis elegans, GI25144369, Length=232, Percent_Identity=26.2931034482759, Blast_Score=84, Evalue=1e-16,
Organism=Caenorhabditis elegans, GI17543114, Length=176, Percent_Identity=28.9772727272727, Blast_Score=74, Evalue=9e-14,
Organism=Saccharomyces cerevisiae, GI6323560, Length=311, Percent_Identity=25.08038585209, Blast_Score=80, Evalue=6e-16,
Organism=Drosophila melanogaster, GI19921524, Length=241, Percent_Identity=25.7261410788382, Blast_Score=75, Evalue=7e-14,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR013785
- InterPro:   IPR001269
- InterPro:   IPR018517 [H]

Pfam domain/function: PF01207 Dus [H]

EC number: NA

Molecular weight: Translated: 37442; Mature: 37442

Theoretical pI: Translated: 5.31; Mature: 5.31

Prosite motif: PS01136 UPF0034

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.6 %Cys     (Translated Protein)
3.4 %Met     (Translated Protein)
4.0 %Cys+Met (Translated Protein)
0.6 %Cys     (Mature Protein)
3.4 %Met     (Mature Protein)
4.0 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MIDNFWRDLPRPFFVLAPMEDVTDVVFRHVVTEAARPDVFFTEFTNSESYCHPDGRQSVR
CCCCHHHHCCCCEEEECCHHHHHHHHHHHHHHHHCCCCEEEEEECCCCCCCCCCCHHHHC
GRLTFTEDEQPIVAHIWGDKPEYFRQMSIGMAEMGFKGLDINMGCPVANVAGNGKGSGLI
CCEEEECCCCCEEEEECCCCHHHHHHHHCCHHHCCCCCCEEECCCCCCCCCCCCCCCCCC
NRPEIAAELIQAAKAGGLPVSVKTRLGYTYIDEWKEWLKHVLEQDIVNLSIHLRTRKEMS
CCHHHHHHHHHHHHCCCCCEEEEHHCCHHHHHHHHHHHHHHHHHHHHEEEEEEECHHHHH
KVDAHWELIPEIKKLRDEIAPDTLLTINGDIPDRQTGLKLVEEYGVDGVMIGRGVFKNPF
HHHHHHHHHHHHHHHHHHHCCCEEEEEECCCCCHHHHHHHHHHHCCCEEEECCCCCCCCC
AFEKEQKEHSSQEMLDLLRLHLDLHDKYDELESRSFKPLHRFFKIYVKGFRGASELRNQM
CCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCHHHHHHHHHHHHHCCCHHHHHHHH
MNTKSTAEVRALLDNFEIENTIEVDQ
HCCCHHHHHHHHHHCCCCCCEEECCC
>Mature Secondary Structure
MIDNFWRDLPRPFFVLAPMEDVTDVVFRHVVTEAARPDVFFTEFTNSESYCHPDGRQSVR
CCCCHHHHCCCCEEEECCHHHHHHHHHHHHHHHHCCCCEEEEEECCCCCCCCCCCHHHHC
GRLTFTEDEQPIVAHIWGDKPEYFRQMSIGMAEMGFKGLDINMGCPVANVAGNGKGSGLI
CCEEEECCCCCEEEEECCCCHHHHHHHHCCHHHCCCCCCEEECCCCCCCCCCCCCCCCCC
NRPEIAAELIQAAKAGGLPVSVKTRLGYTYIDEWKEWLKHVLEQDIVNLSIHLRTRKEMS
CCHHHHHHHHHHHHCCCCCEEEEHHCCHHHHHHHHHHHHHHHHHHHHEEEEEEECHHHHH
KVDAHWELIPEIKKLRDEIAPDTLLTINGDIPDRQTGLKLVEEYGVDGVMIGRGVFKNPF
HHHHHHHHHHHHHHHHHHHCCCEEEEEECCCCCHHHHHHHHHHHCCCEEEECCCCCCCCC
AFEKEQKEHSSQEMLDLLRLHLDLHDKYDELESRSFKPLHRFFKIYVKGFRGASELRNQM
CCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCHHHHHHHHHHHHHCCCHHHHHHHH
MNTKSTAEVRALLDNFEIENTIEVDQ
HCCCHHHHHHHHHHCCCCCCEEECCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 8969503; 9384377 [H]