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The map label for this gene is guaA

Identifier: 23098171

GI number: 23098171

Start: 760794

End: 762329

Strand: Direct

Name: guaA

Synonym: OB0716

Alternate gene names: 23098171

Gene position: 760794-762329 (Clockwise)

Preceding gene: 23098170

Following gene: 23098172

Centisome position: 20.96

GC content: 38.61

Gene sequence:

>1536_bases
ATGGAAAATAATGAGTTAGTACTTGTACTTGATTTTGGAAGTCAATACAATCAGTTAATTACACGTCGAATTAGAGAATT
TGGCGTTTATAGTGAACTTCATTCTCATAAATTAACTGCTGAAGAAATCAAAGAAATGAATCCAAAAGGAATTATTCTTT
CTGGTGGACCGCATAGTGTTTATGATGAAAATAGTTTTCGCTGTGATGAAGGTATTTTTGAATTAGGAATTCCTGTATTA
GGAATTTGTTATGGAATGCAGTTAATGTCACTTCATTATGACGGTAAGGTTGAACGCTCTAAAAACCGTGAGTATGGTAA
AGCATTGATCGAACTAGATGGAGAGCCTGTACTATTTAAAGACACTCCAAAAGGACAGACGGTTTGGATGAGTCATGGAG
ATAAAGTCACTGCAGCACCACCATCTTTCAACATTGATGCAACAAGCCCTTCTACACCAATTGCAGCAATTAGTAATGTA
GAAAAGAACTTGTATGGTGTTCAATTCCATCCAGAAGTTCGCCACACGGAATACGGAAATGATTTATTAAATCGCTTTGT
ATTCGATGTATGCGGATGTACTGGAGATTGGTCAATTGAGAACTTCATTGATATGGAAGTGGAGAAAATTCAAGAAACCG
TTGGCGATCGTAAAGTATTATGTGCGCTAAGTGGTGGAGTAGACTCCTCTGTAGTTGCTGCTTTGATTCATAAAGCGATT
GGTGATCAACTAACTTGTATTTTCGTAGATCACGGTCTTCTTCGTAAAAATGAAGGAGATGATGTCATGAAAGTATTTGC
AGAAGACTTTCAGATGAATATTATCAAAGTCGATGCAAAAGATCGTTTCCTATTTAAATTAGAGGGTGTATCTGACCCAG
AATTAAAACGTAAAATTATTGGTAATGAATTTATCTATGTATTTGATGATGAAGCATCAAAACTGAAAGATATCGACTTC
CTGGCACAAGGTACTCTATATACGGATGTTATTGAAAGTGGTACGGATACAGCGCAAACCATTAAATCTCATCACAACGT
AGGTGGATTACCAGAAGATATGCAGTTTACGCTAATCGAGCCTTTAAATACGTTATTTAAAGATGAAGTTCGTGAACTAG
GATCTCAATTAGGCGTACCTGACCGTATTGTCTGGCGCCAACCATTCCCAGGACCTGGATTAGCTATCCGTATCCTTGGT
GAAGTAACCGAAGAACATTTAGAAATCGTTCGTGAATCGGATGCAATCCTACGTGAAGAAATTGCAAAAGCGGGTCTAGA
TCGTGATATTTGGCAATACTTCACCGTCCTACCGAACATTAAAAGTGTTGGAGTAATGGGGGATGCACGTACGTATGCTC
ATACAATCGGTATCCGTGCGGTAACTTCGATTGATGGAATGACTTCCGATTGGGCACGTATCCCATGGGACGTATTAGAA
AGAATTTCTACACGTCTTGTGAATGATGTAGATAATATTAACCGCGTTGTGTATGACGTAACGAGTAAGCCGCCTGCTAC
GATTGAGTGGGAGTAG

Upstream 100 bases:

>100_bases
GATATAGTCATATACTTTCCAAGGTATACGGTATGCCTACGAAACACAAACTAATAATATCAAAGACAAGCCTATAGTAA
GTATAGATAGGAGAGCGATT

Downstream 100 bases:

>100_bases
AAGAGTGGGAATAGAATTATATAGGAAAATAGGTAAATCATTGGATTGAAGGTTATCTGTGTAGATAGGATAACTGTTCA
CCTGCAACAGTTGTATTTCA

Product: GMP synthase

Products: NA

Alternate protein names: GMP synthetase; Glutamine amidotransferase

Number of amino acids: Translated: 511; Mature: 511

Protein sequence:

>511_residues
MENNELVLVLDFGSQYNQLITRRIREFGVYSELHSHKLTAEEIKEMNPKGIILSGGPHSVYDENSFRCDEGIFELGIPVL
GICYGMQLMSLHYDGKVERSKNREYGKALIELDGEPVLFKDTPKGQTVWMSHGDKVTAAPPSFNIDATSPSTPIAAISNV
EKNLYGVQFHPEVRHTEYGNDLLNRFVFDVCGCTGDWSIENFIDMEVEKIQETVGDRKVLCALSGGVDSSVVAALIHKAI
GDQLTCIFVDHGLLRKNEGDDVMKVFAEDFQMNIIKVDAKDRFLFKLEGVSDPELKRKIIGNEFIYVFDDEASKLKDIDF
LAQGTLYTDVIESGTDTAQTIKSHHNVGGLPEDMQFTLIEPLNTLFKDEVRELGSQLGVPDRIVWRQPFPGPGLAIRILG
EVTEEHLEIVRESDAILREEIAKAGLDRDIWQYFTVLPNIKSVGVMGDARTYAHTIGIRAVTSIDGMTSDWARIPWDVLE
RISTRLVNDVDNINRVVYDVTSKPPATIEWE

Sequences:

>Translated_511_residues
MENNELVLVLDFGSQYNQLITRRIREFGVYSELHSHKLTAEEIKEMNPKGIILSGGPHSVYDENSFRCDEGIFELGIPVL
GICYGMQLMSLHYDGKVERSKNREYGKALIELDGEPVLFKDTPKGQTVWMSHGDKVTAAPPSFNIDATSPSTPIAAISNV
EKNLYGVQFHPEVRHTEYGNDLLNRFVFDVCGCTGDWSIENFIDMEVEKIQETVGDRKVLCALSGGVDSSVVAALIHKAI
GDQLTCIFVDHGLLRKNEGDDVMKVFAEDFQMNIIKVDAKDRFLFKLEGVSDPELKRKIIGNEFIYVFDDEASKLKDIDF
LAQGTLYTDVIESGTDTAQTIKSHHNVGGLPEDMQFTLIEPLNTLFKDEVRELGSQLGVPDRIVWRQPFPGPGLAIRILG
EVTEEHLEIVRESDAILREEIAKAGLDRDIWQYFTVLPNIKSVGVMGDARTYAHTIGIRAVTSIDGMTSDWARIPWDVLE
RISTRLVNDVDNINRVVYDVTSKPPATIEWE
>Mature_511_residues
MENNELVLVLDFGSQYNQLITRRIREFGVYSELHSHKLTAEEIKEMNPKGIILSGGPHSVYDENSFRCDEGIFELGIPVL
GICYGMQLMSLHYDGKVERSKNREYGKALIELDGEPVLFKDTPKGQTVWMSHGDKVTAAPPSFNIDATSPSTPIAAISNV
EKNLYGVQFHPEVRHTEYGNDLLNRFVFDVCGCTGDWSIENFIDMEVEKIQETVGDRKVLCALSGGVDSSVVAALIHKAI
GDQLTCIFVDHGLLRKNEGDDVMKVFAEDFQMNIIKVDAKDRFLFKLEGVSDPELKRKIIGNEFIYVFDDEASKLKDIDF
LAQGTLYTDVIESGTDTAQTIKSHHNVGGLPEDMQFTLIEPLNTLFKDEVRELGSQLGVPDRIVWRQPFPGPGLAIRILG
EVTEEHLEIVRESDAILREEIAKAGLDRDIWQYFTVLPNIKSVGVMGDARTYAHTIGIRAVTSIDGMTSDWARIPWDVLE
RISTRLVNDVDNINRVVYDVTSKPPATIEWE

Specific function: Catalyzes the synthesis of GMP from XMP

COG id: COG0519

COG function: function code F; GMP synthase, PP-ATPase domain/subunit

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 GMP-binding domain

Homologues:

Organism=Homo sapiens, GI4504035, Length=546, Percent_Identity=36.0805860805861, Blast_Score=298, Evalue=9e-81,
Organism=Escherichia coli, GI1788854, Length=516, Percent_Identity=53.2945736434108, Blast_Score=566, Evalue=1e-162,
Organism=Escherichia coli, GI1789760, Length=154, Percent_Identity=34.4155844155844, Blast_Score=70, Evalue=5e-13,
Organism=Caenorhabditis elegans, GI71992710, Length=579, Percent_Identity=37.8238341968912, Blast_Score=323, Evalue=1e-88,
Organism=Caenorhabditis elegans, GI71992717, Length=579, Percent_Identity=37.8238341968912, Blast_Score=323, Evalue=2e-88,
Organism=Caenorhabditis elegans, GI133901714, Length=579, Percent_Identity=37.8238341968912, Blast_Score=322, Evalue=2e-88,
Organism=Saccharomyces cerevisiae, GI6323873, Length=516, Percent_Identity=51.937984496124, Blast_Score=524, Evalue=1e-149,
Organism=Saccharomyces cerevisiae, GI6322638, Length=198, Percent_Identity=28.7878787878788, Blast_Score=66, Evalue=1e-11,
Organism=Drosophila melanogaster, GI281365319, Length=550, Percent_Identity=34.7272727272727, Blast_Score=282, Evalue=5e-76,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): GUAA_OCEIH (Q8CXK8)

Other databases:

- EMBL:   BA000028
- RefSeq:   NP_691637.1
- ProteinModelPortal:   Q8CXK8
- SMR:   Q8CXK8
- GeneID:   1016193
- GenomeReviews:   BA000028_GR
- KEGG:   oih:OB0716
- NMPDR:   fig|221109.1.peg.724
- HOGENOM:   HBG391775
- OMA:   VVNAAHS
- ProtClustDB:   PRK00074
- BioCyc:   OIHE221109:OB0716-MONOMER
- BRENDA:   6.3.5.2
- HAMAP:   MF_00344
- InterPro:   IPR006220
- InterPro:   IPR001317
- InterPro:   IPR011702
- InterPro:   IPR017926
- InterPro:   IPR000991
- InterPro:   IPR001674
- InterPro:   IPR004739
- InterPro:   IPR022955
- InterPro:   IPR022310
- InterPro:   IPR014729
- Gene3D:   G3DSA:3.40.50.620
- PRINTS:   PR00097
- PRINTS:   PR00099
- PRINTS:   PR00096
- TIGRFAMs:   TIGR00884
- TIGRFAMs:   TIGR00888

Pfam domain/function: PF00117 GATase; PF00958 GMP_synt_C; PF02540 NAD_synthase

EC number: =6.3.5.2

Molecular weight: Translated: 57371; Mature: 57371

Theoretical pI: Translated: 4.56; Mature: 4.56

Prosite motif: PS51273 GATASE_TYPE_1; PS00442 GATASE_TYPE_I

Important sites: ACT_SITE 83-83 ACT_SITE 170-170 ACT_SITE 172-172

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.2 %Cys     (Translated Protein)
2.2 %Met     (Translated Protein)
3.3 %Cys+Met (Translated Protein)
1.2 %Cys     (Mature Protein)
2.2 %Met     (Mature Protein)
3.3 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MENNELVLVLDFGSQYNQLITRRIREFGVYSELHSHKLTAEEIKEMNPKGIILSGGPHSV
CCCCCEEEEEECCCHHHHHHHHHHHHHHHHHHHHHCCCCHHHHHHCCCCEEEECCCCCCC
YDENSFRCDEGIFELGIPVLGICYGMQLMSLHYDGKVERSKNREYGKALIELDGEPVLFK
CCCCCCCCCCCHHHHCHHHHHHHHHHHHEEEECCCCCCCCCCHHHCCEEEEECCCEEEEE
DTPKGQTVWMSHGDKVTAAPPSFNIDATSPSTPIAAISNVEKNLYGVQFHPEVRHTEYGN
CCCCCCEEEEECCCEEEECCCCCCCCCCCCCCCHHHHHHHHHHEEEEEECCCCCCCCCCH
DLLNRFVFDVCGCTGDWSIENFIDMEVEKIQETVGDRKVLCALSGGVDSSVVAALIHKAI
HHHHHHHHHHHCCCCCCCHHHHHHHHHHHHHHHHCCCEEEEEECCCCCHHHHHHHHHHHH
GDQLTCIFVDHGLLRKNEGDDVMKVFAEDFQMNIIKVDAKDRFLFKLEGVSDPELKRKII
CCCEEEEEEECCCCCCCCCHHHHHHHHHHHCEEEEEEECCCEEEEEECCCCCHHHHHHHC
GNEFIYVFDDEASKLKDIDFLAQGTLYTDVIESGTDTAQTIKSHHNVGGLPEDMQFTLIE
CCCEEEEECCCCHHHHHHHHHHCCCHHHHHHHCCCCHHHHHHHHCCCCCCCCCCCEEHHH
PLNTLFKDEVRELGSQLGVPDRIVWRQPFPGPGLAIRILGEVTEEHLEIVRESDAILREE
HHHHHHHHHHHHHHHHCCCCCEEEECCCCCCCCCEEHHHHHHHHHHHHHHHHHHHHHHHH
IAKAGLDRDIWQYFTVLPNIKSVGVMGDARTYAHTIGIRAVTSIDGMTSDWARIPWDVLE
HHHCCCCHHHHHHHHHCCCCHHCCCCCCCHHHHHHHHHEEEECCCCCCCCHHCCCHHHHH
RISTRLVNDVDNINRVVYDVTSKPPATIEWE
HHHHHHHHHHHHHCEEEEEECCCCCCEEECC
>Mature Secondary Structure
MENNELVLVLDFGSQYNQLITRRIREFGVYSELHSHKLTAEEIKEMNPKGIILSGGPHSV
CCCCCEEEEEECCCHHHHHHHHHHHHHHHHHHHHHCCCCHHHHHHCCCCEEEECCCCCCC
YDENSFRCDEGIFELGIPVLGICYGMQLMSLHYDGKVERSKNREYGKALIELDGEPVLFK
CCCCCCCCCCCHHHHCHHHHHHHHHHHHEEEECCCCCCCCCCHHHCCEEEEECCCEEEEE
DTPKGQTVWMSHGDKVTAAPPSFNIDATSPSTPIAAISNVEKNLYGVQFHPEVRHTEYGN
CCCCCCEEEEECCCEEEECCCCCCCCCCCCCCCHHHHHHHHHHEEEEEECCCCCCCCCCH
DLLNRFVFDVCGCTGDWSIENFIDMEVEKIQETVGDRKVLCALSGGVDSSVVAALIHKAI
HHHHHHHHHHHCCCCCCCHHHHHHHHHHHHHHHHCCCEEEEEECCCCCHHHHHHHHHHHH
GDQLTCIFVDHGLLRKNEGDDVMKVFAEDFQMNIIKVDAKDRFLFKLEGVSDPELKRKII
CCCEEEEEEECCCCCCCCCHHHHHHHHHHHCEEEEEEECCCEEEEEECCCCCHHHHHHHC
GNEFIYVFDDEASKLKDIDFLAQGTLYTDVIESGTDTAQTIKSHHNVGGLPEDMQFTLIE
CCCEEEEECCCCHHHHHHHHHHCCCHHHHHHHCCCCHHHHHHHHCCCCCCCCCCCEEHHH
PLNTLFKDEVRELGSQLGVPDRIVWRQPFPGPGLAIRILGEVTEEHLEIVRESDAILREE
HHHHHHHHHHHHHHHHCCCCCEEEECCCCCCCCCEEHHHHHHHHHHHHHHHHHHHHHHHH
IAKAGLDRDIWQYFTVLPNIKSVGVMGDARTYAHTIGIRAVTSIDGMTSDWARIPWDVLE
HHHCCCCHHHHHHHHHCCCCHHCCCCCCCHHHHHHHHHEEEECCCCCCCCHHCCCHHHHH
RISTRLVNDVDNINRVVYDVTSKPPATIEWE
HHHHHHHHHHHHHCEEEEEECCCCCCEEECC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 12235376