Click here to switch to the map view.

The map label for this gene is nylA [H]

Identifier: 23098166

GI number: 23098166

Start: 752400

End: 753908

Strand: Direct

Name: nylA [H]

Synonym: OB0711

Alternate gene names: 23098166

Gene position: 752400-753908 (Clockwise)

Preceding gene: 23098160

Following gene: 23098167

Centisome position: 20.72

GC content: 37.24

Gene sequence:

>1509_bases
ATGAATTTTAATGAATATATATCCCATGATGCTATCGGGTTAGCGAAATTAATCAAGAACAAACAAGTACATGCCAATGA
GTTAATTAATTTGGCGTTTAATAGATTAAATGAAGTAAATGACGAATTGAATATTATTACGCATAGCCGTGAAGAACGAG
TGAAAAAAGAAGCTCTAAATGAAATAACAATTCCGAATGGCATGTTTCAAGGGGTTCCGATTTTGATGAAGAATATTTCA
CAAGCTATTGAAGGGGAGCCAATGTCATCAGGGTCTAAACTTTTAAAAGATGTTACATATAAACACGATAACTATTTTGT
TAAGAAATTTAGAGAAGCTGGTTTTTTATTTATGGGGCATACAAATACGCCAGAATTTGGTTTAAAAAATATAACAGAGC
CACAGTTATATGGAGCAACACATAATCCTTGGAATACATTACATTCGCCTGGTGGTTCAAGTGGAGGTGCGGCTGCAGCT
ATTGCTTCTGGTGTAGTGCCTTTAGCAGGTGCAAGTGATGGTGGAGGATCCATCCGTATTCCTGCATCATTTACAGGACT
TTTTGGAATAAAACCAACTCGTGGACGAACTCCAGTTGGCCCTGGGGTGGGTAGGCAGTGGCAGGGAGCTTCCATTAACT
TTGTTTTAAGTCGTTCCGTTCGTGATAGTGCAGCTATGTTAGATCAATTGCAAGTGGTAGAGCCTCATGCTGCCTTTCAG
ACACCATTATTTGCAGGCGGATATTTAAACGATATTGAAAAGTCACCGGAAAAGTCGTTGAGAATAGCATATTCTACTGA
ATCACCGGTAGGTACTCCCGTATCTGAAGACGCAAAAGAAGCAGTAAATAACTTAGTACAGTTATTAAGTAATGAAGGAC
ATGATCTTGAAGAAGTGCAAGCTCCTATTGATGGAAGGCAATTGATGAATGATTACTACATAATGAATAGTGGAGAGATG
AATGCAACTATTTTAGGTATAGAAAATATGCTTAACCGAAAATTAACTGATGATGACATGGAAATAGAGTCTTGGTTATT
ACATCGAGCAGGAGAATCGGTAAGTGCAGCAGCTTATTCACAAAGTTTGTCTTCATGGGATTTAGCAGCCGAGAAAATGG
CTTATTTTCACCAAACATATGATTTCTACATCACACCTTCAACTGCTTATTCAGCACCTAGAGTTGGGGAATTAACAATG
ACTAAAGAGAAGCAAGAGGATTATAAAGAATGCATAGAGAAGGCATCTGCTAGAGAAAAGCAATCCATTATTTATGATAT
GTTTTTACCGAGTTTAACGTACACGCCTTTTACACAACTTGCCAATTTAACTGGACAGCCTGCTATGTCATTACCACTTT
ATTTAACTGAACAAGGACTTCCTTTAGGAGTTCAGGTTATGGCAAATAAAGGAGAAGAACACCGATTACTACGATTTGCT
CGTTATCTGGAACAATCTAATTTATGGGTAAAAGTGAACGAGAATCCTTATTTTAATATATCAAATTAA

Upstream 100 bases:

>100_bases
GTTTTATTAAAATTTTATATTTTTATTATTTTAGTAGAGGCTGACTAATTTATTTAATAATGGTAGAATTTGGTTAAAGT
TGTAGGAGATGAAATAGTAG

Downstream 100 bases:

>100_bases
CATAAAATAGTAGCATTTTTCGACAATCTTATGAAAAATGCTACTATTTTTACTTTTAGGTAATAGGTTTCATTATTATC
TGTTCGAATGTATAATGAAA

Product: 6-aminohexanoate-cyclic-dimer hydrolase

Products: NA

Alternate protein names: Nylon oligomers-degrading enzyme EI [H]

Number of amino acids: Translated: 502; Mature: 502

Protein sequence:

>502_residues
MNFNEYISHDAIGLAKLIKNKQVHANELINLAFNRLNEVNDELNIITHSREERVKKEALNEITIPNGMFQGVPILMKNIS
QAIEGEPMSSGSKLLKDVTYKHDNYFVKKFREAGFLFMGHTNTPEFGLKNITEPQLYGATHNPWNTLHSPGGSSGGAAAA
IASGVVPLAGASDGGGSIRIPASFTGLFGIKPTRGRTPVGPGVGRQWQGASINFVLSRSVRDSAAMLDQLQVVEPHAAFQ
TPLFAGGYLNDIEKSPEKSLRIAYSTESPVGTPVSEDAKEAVNNLVQLLSNEGHDLEEVQAPIDGRQLMNDYYIMNSGEM
NATILGIENMLNRKLTDDDMEIESWLLHRAGESVSAAAYSQSLSSWDLAAEKMAYFHQTYDFYITPSTAYSAPRVGELTM
TKEKQEDYKECIEKASAREKQSIIYDMFLPSLTYTPFTQLANLTGQPAMSLPLYLTEQGLPLGVQVMANKGEEHRLLRFA
RYLEQSNLWVKVNENPYFNISN

Sequences:

>Translated_502_residues
MNFNEYISHDAIGLAKLIKNKQVHANELINLAFNRLNEVNDELNIITHSREERVKKEALNEITIPNGMFQGVPILMKNIS
QAIEGEPMSSGSKLLKDVTYKHDNYFVKKFREAGFLFMGHTNTPEFGLKNITEPQLYGATHNPWNTLHSPGGSSGGAAAA
IASGVVPLAGASDGGGSIRIPASFTGLFGIKPTRGRTPVGPGVGRQWQGASINFVLSRSVRDSAAMLDQLQVVEPHAAFQ
TPLFAGGYLNDIEKSPEKSLRIAYSTESPVGTPVSEDAKEAVNNLVQLLSNEGHDLEEVQAPIDGRQLMNDYYIMNSGEM
NATILGIENMLNRKLTDDDMEIESWLLHRAGESVSAAAYSQSLSSWDLAAEKMAYFHQTYDFYITPSTAYSAPRVGELTM
TKEKQEDYKECIEKASAREKQSIIYDMFLPSLTYTPFTQLANLTGQPAMSLPLYLTEQGLPLGVQVMANKGEEHRLLRFA
RYLEQSNLWVKVNENPYFNISN
>Mature_502_residues
MNFNEYISHDAIGLAKLIKNKQVHANELINLAFNRLNEVNDELNIITHSREERVKKEALNEITIPNGMFQGVPILMKNIS
QAIEGEPMSSGSKLLKDVTYKHDNYFVKKFREAGFLFMGHTNTPEFGLKNITEPQLYGATHNPWNTLHSPGGSSGGAAAA
IASGVVPLAGASDGGGSIRIPASFTGLFGIKPTRGRTPVGPGVGRQWQGASINFVLSRSVRDSAAMLDQLQVVEPHAAFQ
TPLFAGGYLNDIEKSPEKSLRIAYSTESPVGTPVSEDAKEAVNNLVQLLSNEGHDLEEVQAPIDGRQLMNDYYIMNSGEM
NATILGIENMLNRKLTDDDMEIESWLLHRAGESVSAAAYSQSLSSWDLAAEKMAYFHQTYDFYITPSTAYSAPRVGELTM
TKEKQEDYKECIEKASAREKQSIIYDMFLPSLTYTPFTQLANLTGQPAMSLPLYLTEQGLPLGVQVMANKGEEHRLLRFA
RYLEQSNLWVKVNENPYFNISN

Specific function: Specifically catalyzes the hydrolysis of 6-aminohexanoic acid cyclic dimer (1,8-diazacyclotetradecane-2,9-dione) to form the linear dimer 6-aminohexanoyl-6-aminohexanoic acid. Is inactive on 6-aminohexanoic acid oligomers (degree of polymerization 2 to 6)

COG id: COG0154

COG function: function code J; Asp-tRNAAsn/Glu-tRNAGln amidotransferase A subunit and related amidases

Gene ontology:

Cell location: Cytoplasmic

Metaboloic importance: NA

Operon status: Not Known

Operon components: None

Similarity: Belongs to the amidase family [H]

Homologues:

Organism=Homo sapiens, GI195972892, Length=197, Percent_Identity=35.5329949238579, Blast_Score=108, Evalue=1e-23,
Organism=Homo sapiens, GI222831590, Length=521, Percent_Identity=24.3761996161228, Blast_Score=89, Evalue=7e-18,
Organism=Caenorhabditis elegans, GI17543272, Length=471, Percent_Identity=24.416135881104, Blast_Score=96, Evalue=4e-20,
Organism=Caenorhabditis elegans, GI17556264, Length=311, Percent_Identity=27.0096463022508, Blast_Score=92, Evalue=8e-19,
Organism=Caenorhabditis elegans, GI17537465, Length=299, Percent_Identity=26.4214046822742, Blast_Score=86, Evalue=6e-17,
Organism=Caenorhabditis elegans, GI17556276, Length=201, Percent_Identity=32.8358208955224, Blast_Score=80, Evalue=3e-15,
Organism=Caenorhabditis elegans, GI17556278, Length=201, Percent_Identity=32.8358208955224, Blast_Score=79, Evalue=4e-15,
Organism=Caenorhabditis elegans, GI17538252, Length=188, Percent_Identity=28.7234042553192, Blast_Score=74, Evalue=2e-13,
Organism=Caenorhabditis elegans, GI71990152, Length=246, Percent_Identity=29.6747967479675, Blast_Score=71, Evalue=1e-12,
Organism=Drosophila melanogaster, GI45550774, Length=196, Percent_Identity=34.6938775510204, Blast_Score=100, Evalue=4e-21,
Organism=Drosophila melanogaster, GI24648435, Length=196, Percent_Identity=34.6938775510204, Blast_Score=99, Evalue=5e-21,
Organism=Drosophila melanogaster, GI24648437, Length=196, Percent_Identity=34.6938775510204, Blast_Score=99, Evalue=5e-21,
Organism=Drosophila melanogaster, GI24648113, Length=454, Percent_Identity=27.5330396475771, Blast_Score=98, Evalue=1e-20,
Organism=Drosophila melanogaster, GI21356731, Length=243, Percent_Identity=29.6296296296296, Blast_Score=92, Evalue=1e-18,
Organism=Drosophila melanogaster, GI24652985, Length=526, Percent_Identity=25.2851711026616, Blast_Score=89, Evalue=6e-18,
Organism=Drosophila melanogaster, GI19922090, Length=526, Percent_Identity=25.2851711026616, Blast_Score=89, Evalue=6e-18,
Organism=Drosophila melanogaster, GI24652981, Length=526, Percent_Identity=25.2851711026616, Blast_Score=89, Evalue=6e-18,
Organism=Drosophila melanogaster, GI24652983, Length=526, Percent_Identity=25.2851711026616, Blast_Score=89, Evalue=6e-18,
Organism=Drosophila melanogaster, GI161078093, Length=503, Percent_Identity=23.6580516898608, Blast_Score=86, Evalue=9e-17,
Organism=Drosophila melanogaster, GI24644968, Length=210, Percent_Identity=28.0952380952381, Blast_Score=79, Evalue=6e-15,
Organism=Drosophila melanogaster, GI24648441, Length=137, Percent_Identity=37.2262773722628, Blast_Score=79, Evalue=8e-15,
Organism=Drosophila melanogaster, GI24648439, Length=137, Percent_Identity=37.2262773722628, Blast_Score=79, Evalue=8e-15,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR000120
- InterPro:   IPR020556 [H]

Pfam domain/function: PF01425 Amidase [H]

EC number: =3.5.2.12 [H]

Molecular weight: Translated: 55553; Mature: 55553

Theoretical pI: Translated: 5.34; Mature: 5.34

Prosite motif: PS00571 AMIDASES

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.2 %Cys     (Translated Protein)
3.2 %Met     (Translated Protein)
3.4 %Cys+Met (Translated Protein)
0.2 %Cys     (Mature Protein)
3.2 %Met     (Mature Protein)
3.4 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MNFNEYISHDAIGLAKLIKNKQVHANELINLAFNRLNEVNDELNIITHSREERVKKEALN
CCCHHHHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHCCCHHEEEECCHHHHHHHHHHH
EITIPNGMFQGVPILMKNISQAIEGEPMSSGSKLLKDVTYKHDNYFVKKFREAGFLFMGH
HCCCCCCCCCCHHHHHHHHHHHHCCCCCCCHHHHHHHHHHCCCHHHHHHHHHCCEEEEEC
TNTPEFGLKNITEPQLYGATHNPWNTLHSPGGSSGGAAAAIASGVVPLAGASDGGGSIRI
CCCCCCCCCCCCCCHHCCCCCCCCHHCCCCCCCCCCHHHHHHHCCCEECCCCCCCCEEEE
PASFTGLFGIKPTRGRTPVGPGVGRQWQGASINFVLSRSVRDSAAMLDQLQVVEPHAAFQ
CCCCCEEECCCCCCCCCCCCCCCCCCCCCCCEEEEEECCHHHHHHHHHHHHHCCCCHHHC
TPLFAGGYLNDIEKSPEKSLRIAYSTESPVGTPVSEDAKEAVNNLVQLLSNEGHDLEEVQ
CCCCCCCCHHHHHCCCCCCEEEEEECCCCCCCCCCHHHHHHHHHHHHHHCCCCCCHHHHH
APIDGRQLMNDYYIMNSGEMNATILGIENMLNRKLTDDDMEIESWLLHRAGESVSAAAYS
CCCCHHHHHCCEEEEECCCCCEEEEEHHHHHCCCCCCCHHHHHHHHHHHCCCCHHHHHHH
QSLSSWDLAAEKMAYFHQTYDFYITPSTAYSAPRVGELTMTKEKQEDYKECIEKASAREK
HHHHHHHHHHHHHHHHHHCEEEEECCCCCCCCCCCCCEEECHHHHHHHHHHHHHHHHHHH
QSIIYDMFLPSLTYTPFTQLANLTGQPAMSLPLYLTEQGLPLGVQVMANKGEEHRLLRFA
HHHHHHHHHCCCCCCCHHHHHCCCCCCCCCCCEEEECCCCCEEEEEEECCCCHHHHHHHH
RYLEQSNLWVKVNENPYFNISN
HHHHHCCCEEEECCCCCEECCC
>Mature Secondary Structure
MNFNEYISHDAIGLAKLIKNKQVHANELINLAFNRLNEVNDELNIITHSREERVKKEALN
CCCHHHHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHCCCHHEEEECCHHHHHHHHHHH
EITIPNGMFQGVPILMKNISQAIEGEPMSSGSKLLKDVTYKHDNYFVKKFREAGFLFMGH
HCCCCCCCCCCHHHHHHHHHHHHCCCCCCCHHHHHHHHHHCCCHHHHHHHHHCCEEEEEC
TNTPEFGLKNITEPQLYGATHNPWNTLHSPGGSSGGAAAAIASGVVPLAGASDGGGSIRI
CCCCCCCCCCCCCCHHCCCCCCCCHHCCCCCCCCCCHHHHHHHCCCEECCCCCCCCEEEE
PASFTGLFGIKPTRGRTPVGPGVGRQWQGASINFVLSRSVRDSAAMLDQLQVVEPHAAFQ
CCCCCEEECCCCCCCCCCCCCCCCCCCCCCCEEEEEECCHHHHHHHHHHHHHCCCCHHHC
TPLFAGGYLNDIEKSPEKSLRIAYSTESPVGTPVSEDAKEAVNNLVQLLSNEGHDLEEVQ
CCCCCCCCHHHHHCCCCCCEEEEEECCCCCCCCCCHHHHHHHHHHHHHHCCCCCCHHHHH
APIDGRQLMNDYYIMNSGEMNATILGIENMLNRKLTDDDMEIESWLLHRAGESVSAAAYS
CCCCHHHHHCCEEEEECCCCCEEEEEHHHHHCCCCCCCHHHHHHHHHHHCCCCHHHHHHH
QSLSSWDLAAEKMAYFHQTYDFYITPSTAYSAPRVGELTMTKEKQEDYKECIEKASAREK
HHHHHHHHHHHHHHHHHHCEEEEECCCCCCCCCCCCCEEECHHHHHHHHHHHHHHHHHHH
QSIIYDMFLPSLTYTPFTQLANLTGQPAMSLPLYLTEQGLPLGVQVMANKGEEHRLLRFA
HHHHHHHHHCCCCCCCHHHHHCCCCCCCCCCCEEEECCCCCEEEEEEECCCCHHHHHHHH
RYLEQSNLWVKVNENPYFNISN
HHHHHCCCEEEECCCCCEECCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 2722746 [H]